-
No products found
because this supplier's products are not listed.
Karen. M. Marshall, et al.,
bioRxiv - Bioengineering 2023
Quote:
... the scaffolds seeded with C2C12 cells were moved to a 24 well plate and 1.5 mL of media was added (basal with 2% FCS ± 5 µg of BMP-2 (3.33 µg/mL BMP-2). Culture was continued for 4 days and ALP staining performed on the 5th day from cell seeding ...
-
No products found
because this supplier's products are not listed.
Zhimin Huang, et al.,
bioRxiv - Bioengineering 2023
Quote:
... (2) block sections in 5% normal goat serum (SouthernBiotech) and 0.3% Triton-X solution in PBS for 1h at room temperature ...
-
No products found
because this supplier's products are not listed.
Shreyas Krishnan, Richard L. Cryberg,
bioRxiv - Genetics 2019
Quote:
... 5 μL FailSafe 2× PCR premix G (EpiCentre, Madison, Wisconsin), approximately 25 ng genomic DNA template ...
-
No products found
because this supplier's products are not listed.
Adele Xu, et al.,
bioRxiv - Genetics 2022
Quote:
... 5-ethynyl-2’-deoxyuridine (EdU) (Click Chemistry Tools 1381, Scottsdale, AZ) was added to a final concentration of 10 uM ...
-
No products found
because this supplier's products are not listed.
Shinya Ito, et al.,
bioRxiv - Neuroscience 2020
Quote:
We used 2–5 month-old CBA/CaJ (The Jackson Laboratory, 000654) mice of each sex ...
-
No products found
because this supplier's products are not listed.
Anthony R. Anzell, et al.,
bioRxiv - Developmental Biology 2024
Quote:
... 5% CO2 in endothelial growth medium 2 (EGM2, consisting of endothelial basal medium-2 supplemented with growth factors; Promocell) and 1X antibiotic-antimycotic (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Rayan Khaddaj-Mallat, et al.,
bioRxiv - Neuroscience 2021
Quote:
Pericytes viability was assessed using the 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay according to the manufacturer’s procedure (Cell Signaling Technology). Cells were plated at the appropriate concentration in 96-well plates under different experimental conditions ...
-
No products found
because this supplier's products are not listed.
Ana Domingo-Muelas, et al.,
bioRxiv - Neuroscience 2022
Quote:
... 2+5 DIV differentiation samples were imaged using a Nikon ECLIPSE Ni-U microscope (Nikon) with a Zyla 4.2 sCMOS camera (Andor) ...
-
No products found
because this supplier's products are not listed.
Benjamin Demaree, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... 2 million cells were added to a 5 mL DNA LoBind tube (Eppendorf, cat. no. 0030108310), centrifuged at 400 x g for 4 min ...
-
No products found
because this supplier's products are not listed.
Sayantan Roy, Bing Wang, Yuan Tian, Qian Yin,
bioRxiv - Biochemistry 2023
Quote:
... 5 mM MgCl2 and 2 mM Tris (2-carboxyethyl) phosphine (TCEP, GoldBio).
-
No products found
because this supplier's products are not listed.
Ana Cláudia Raposo, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... 5-(hydroxymethyl)-2′-deoxycytidine-2H3 (#H946632, Toronto Research Chemicals). MS2 data for 5hmC ...
-
No products found
because this supplier's products are not listed.
Sherman Qu, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... [15N]5-2-deoxyadenosine was obtained from Cambridge Isotope Laboratories ...
-
No products found
because this supplier's products are not listed.
Juan Yang, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Sulfo-Cyanine 3 Azide (2-5 uM final, Lumiprobe, #D1330), and fresh Sodium Ascorbate (100 mM final ...
-
No products found
because this supplier's products are not listed.
Thi Tuyet Trinh Phan, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... MTT (3-(4,5-dimethylthiazol-2-yl-2, 5-diphenyl tetrazolium bromide)) was purchased from Alfa Aesar (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Samuel Hume, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... and using 5 μg anti-NUCKS1 antibody (ProteinTech 12023-2-AP) or 5 μg normal rabbit IgG (SantaCruz sc2027) ...
-
No products found
because this supplier's products are not listed.
Dillon S. McDevitt, et al.,
bioRxiv - Neuroscience 2019
Quote:
... recording electrodes (2-5 MΩ; borosilicate glass capillaries (WPI #1B150F-4) pulled on a horizontal puller from Sutter Instruments (model P-97) ...
-
No products found
because this supplier's products are not listed.
Dougald M. Monroe, et al.,
bioRxiv - Biochemistry 2022
Quote:
... 4 μM lipid vesicles (Avanti Polar Lipids PS:PC:PE 3:2:5), and FV (0 ...
-
No products found
because this supplier's products are not listed.
Jose Ernesto Canton-Josh, et al.,
bioRxiv - Neuroscience 2022
Quote:
... 2-5 mW) delivered through a 60X water-immersion objective (Olympus, Tokyo, Japan) with CoolLED PE4000 widefield illumination (CoolLED Ltd. ...
-
No products found
because this supplier's products are not listed.
Hooi Ting Hu, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Cells (5 × 105) were seeded into a well of culture-insert 2 well (ibidi, 80209). The cells were then cultured for 7 days without replacement of the medium and visualized using crystal violet staining.
-
No products found
because this supplier's products are not listed.
Yoichi Araki, et al.,
bioRxiv - Neuroscience 2020
Quote:
... or Spinning disk confocal microscopes controlled by axiovision software (Carl Zeiss; Fig. 2, 3, and 5). Following 5-10 min of baseline recording ...
-
Prepared to contain higher collagenase and caseinase activities. A dialyzed, lyophilized powder.
Cat# LS005282,
1 gm, $246.00
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Luke R. Perreault, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Minced tissue underwent 3-5 serial digestions in collagenase type 2 (Worthington Biochemical Corp, Lakewood, NJ) in sterile PBS with 20 mM glucose ...
-
No products found
because this supplier's products are not listed.
Andrew G. Shuparski, et al.,
bioRxiv - Immunology 2021
Quote:
... cells were incubated for 5 mins with 2% (vol/vol) normal mouse and normal rat serum (Jackson ImmunoResearch Laboratories), prior to staining with a 2X master mix containing fluorophore-labeled antibodies at pre-titrated concentrations in Brilliant Violet Staining Buffer (BD Biosciences) ...
-
No products found
because this supplier's products are not listed.
Alexandros Sfikas, et al.,
bioRxiv - Cell Biology 2019
Quote:
... siRNA 2 5’ GCCCUAUCCCUUUACGUCA (Eurogentec). Dharmacon ONTarget plus SMARTpool ...
-
No products found
because this supplier's products are not listed.
Karol Estrada, et al.,
bioRxiv - Genetics 2020
Quote:
... cells were then incubated in DMEM containing 0.75 mM IBMX (3-isobutyl-1-methylxanthine) (Enzo life sciences) for 15 min ...
-
No products found
because this supplier's products are not listed.
Preksha Gupta, et al.,
bioRxiv - Bioengineering 2023
Quote:
... Rainbow Calibration particles (RCP-60-5-2, RCP-60-5-5) were obtained from Spherotech. FITC and PE stained beads were part of the three color BD CaliBRITE kit (BD Biosciences 340486) ...
-
No products found
because this supplier's products are not listed.
Yang Wang, et al.,
bioRxiv - Microbiology 2024
Quote:
... and IL-5 (Mabtech, 3391-4HPW-2) precoated ELISpot kits according to the manufacturer’s instructions ...
-
Butyl isobutyl phthalate (BIP), isolated from the rhizoid of Laminaria japonica, is a...
Cat# S0553, SKU# S0553-25mg,
25mg, $87.00
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Ece Ergir, et al.,
bioRxiv - Cell Biology 2022
Quote:
... supplemented with IWP-2 (5 μM, Selleck chemicals). On day 4 ...
-
No products found
because this supplier's products are not listed.
Yi Juin Liew, et al.,
bioRxiv - Neuroscience 2021
Quote:
Tungsten microelectrodes (FHC, impedance: 2-5 MΩ, 75 µm in diameter) were used in thalamus and cortex to isolate single units that were responding to a single primary whisker on the contralateral side of the face ...
-
No products found
because this supplier's products are not listed.
Shristi Baral, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Borosilicate glass pipettes (Sutter Instrument BF100-58-10, 2-5 MΩ) were pulled using a laser micropipette puller (Sutter Instrument Model P-2000) ...
-
No products found
because this supplier's products are not listed.
Yiran Li, et al.,
bioRxiv - Genomics 2020
Quote:
... 2 □1 water and 5□1 MyTaq™ HS Mix (Bioline). RT-PCR was performed in the following conditions ...
-
No products found
because this supplier's products are not listed.
Daniel R. Lu, et al.,
bioRxiv - Immunology 2019
Quote:
... with 5 μg purified anti-mouse IL-2 antibody (JES6-1, BioXcell) for 30 min at 37°C (molar ratio for IL2:anti-IL-2 is 2:1) ...
-
No products found
because this supplier's products are not listed.
Allie Lipshutz, et al.,
bioRxiv - Neuroscience 2022
Quote:
... angle 0°) under a combined isoflurane anesthetic (2-5%, SomnoSuite, Kent Scientific) and meloxicam analgesic (3 mg/kg ...
-
No products found
because this supplier's products are not listed.
Yoon-Hee Jung, et al.,
bioRxiv - Genomics 2022
Quote:
Adult (2-5 months old) CD-1 IGS mice (Charles River Lab, USA) were used for all experiments ...
-
No products found
because this supplier's products are not listed.
Sneha Damal Villivalam, et al.,
bioRxiv - Cell Biology 2020
Quote:
... 600 ng of denatured DNA was incubated with 2 mg of 5-methylcytosine (5-mC) monoclonal antibody ([33D3] Diagenode, Cat No # C15200081) in IP buffer (0.1% SDS ...
-
No products found
because this supplier's products are not listed.
Amanuel Tafessu, et al.,
bioRxiv - Genomics 2022
Quote:
... 5 μg of antibody and 2 µg of spike-in antibody (Active Motif 61686) were bound to 50 μl protein A or protein G Dynabeads (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Melanie A. MacMullan, et al.,
bioRxiv - Immunology 2022
Quote:
... Beads were then reconstituted in 200 µL elution buffer 2 (EB2, 5% ACN, 0.3M NH4OH) and incubated for 1 hour on a ThermoMixer at 45°C ...
-
No products found
because this supplier's products are not listed.
Michihito Sasaki, et al.,
bioRxiv - Microbiology 2022
Quote:
... followed by permeabilization with 0.5% Triton X-100 in PBS for 5 min and staining with anti-SARS-CoV-2 nucleocapsid rabbit monoclonal antibody (GTX635679, GeneTex) in 25% Block Ace (KAC ...
-
No products found
because this supplier's products are not listed.
Neus Pedraza, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... filtered at 2 kHz and digitized at 5 kHz using Digidata 1440A interface with pClamp 10 software (Molecular Devices Corporation). Series resistance was typically 5–20 MΩ ...
-
No products found
because this supplier's products are not listed.
Grace I. Borlee, et al.,
bioRxiv - Microbiology 2021
Quote:
... supplemented with 100 nM of 2-chloro-adenosine-5’-O-monophsphate (2-Cl-5’-AMP, Axxora, LLC) for internal standardization ...
-
No products found
because this supplier's products are not listed.
Houjian Zhang, et al.,
bioRxiv - Neuroscience 2022
Quote:
... 5-ethynyl-2’-deoxyuridine (EdU) (APExBIO, Cat. No. B8337) was injected intraperitoneally at a concentration of 50 mg/kg body weight and mice were euthanized by cervical dislocation 12 or 24 h later.
-
No products found
because this supplier's products are not listed.
Przemyslaw Jarzebowski, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Mice were anesthetized with isoflurane (5% induction, 1-2% maintenance, Abbott Ltd, Maidenhead, UK) mixed with oxygen as carrier gas (flow rate 1.0-2.0 L.min-1 ...
-
No products found
because this supplier's products are not listed.
Rowyn C Liebau, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... Membranes were then blocked for 1-2 hours in 5% powdered milk (Amresco, M203-10G-10PK) in PBST ...
-
No products found
because this supplier's products are not listed.
Giorgia Fedele, et al.,
bioRxiv - Cell Biology 2022
Quote:
... 5 U/mL Heparin and 150 μg/mL Endothelial Cell Growth Factor on 2% gelatin-coated dishes (Euroclone). Human astrocytes (HA ...
-
No products found
because this supplier's products are not listed.
Francesca Querques, et al.,
bioRxiv - Cell Biology 2021
Quote:
... human TCEAL4 transcript variant 1) and pCMV6-TCEAL4 isoform-2 (CAT#: SC335597, NM_001300901, human TCEAL4 transcript variant 5) were both from Origene. TCEAL4 isoform-1 was cloned from pCMV6-TCEAL4-MYC-FLAG with the Gateway cloning system into pDONR223 and then into the destination vector pEGFP_GW ...
-
No products found
because this supplier's products are not listed.
Jiaqi Zhu, Yue Su, Young Tang,
bioRxiv - Molecular Biology 2022
Quote:
... The membrane was blocked by 5% skim milk and incubated with ACE-2 Antibody (1:2,000, Novus Biologicals, CO, USA), 6xHN Polyclonal Antibody (1:2000 ...
-
No products found
because this supplier's products are not listed.
Evan M. Hess, et al.,
bioRxiv - Neuroscience 2022
Quote:
... supplemented with 5% fetal bovine serum/5% horse serum (Atlanta Biologicals), Glutamax (Gibco) ...
-
No products found
because this supplier's products are not listed.
Diane Maurice, et al.,
bioRxiv - Immunology 2023
Quote:
... then cultured with 20ng/ml recombinant IL-2 for an additional 5 days (rmIL-2, Immunotools). CD8 effector cells were mixed with CFSE-stained EL4 target cells ...
-
No products found
because this supplier's products are not listed.
Varun Tiwari, et al.,
bioRxiv - Biophysics 2020
Quote:
... 5 μg/μL leupeptin and 2% n-dodecyl-β-D-maltopyranoside (DDM, Anatrace), mixed using a Dounce homogenizer and incubated for ~16 hr at 4°C with agitation ...
-
No products found
because this supplier's products are not listed.
Tyler Fair, et al.,
bioRxiv - Genetics 2023
Quote:
... resuspended in v3.1 medium supplemented with 2 μM thiazovivin and 5 μg/ml polybrene (Mirus, MIR 6620), transduced with the hDel-v1 or hDel-v2 lentiviral sgRNA library at a target infection rate of 25% ...
-
No products found
because this supplier's products are not listed.
Saffron T.G. Whitta, et al.,
bioRxiv - Microbiology 2024
Quote:
... Plate was then incubated at 37°C + 5% CO2 for 2 hours before 60% of the wells’ supernatant was carefully transferred into a black-well plate (Greiner, #655076). A VarioskanTM LUX plate reader (Thermo Fisher Scientific #VL0000D0 ...