-
No products found
because this supplier's products are not listed.
Shayan Shamipour, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... Prior to start of imaging 6.5 ml culture medium with 1 μg/ml 17alpha,20beta-Dihydroxy-4-pregnen-3-one (DHP, diluted in 100% Ethanol, Sigma) containing drug/Lysotracker was added on top of the solidified LMP agarose ...
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No products found
because this supplier's products are not listed.
Fabian M. Meyer, et al.,
bioRxiv - Microbiology 2023
Quote:
... cells were resuspended in 25 µl PBS together with 0.25 µl of 5 mM 3-[[(7-Hydroxy-2-oxo-2H-1-benzopyran-3-yl) carbonyl]amino]-D-alanine hydrocholoride (HADA) (Tocris) dissolved in DMSO ...
-
No products found
because this supplier's products are not listed.
Vamsee D Myneni, et al.,
bioRxiv - Immunology 2024
Quote:
... or N′-((4-oxo-4H-chromen-3-yl) methylene) nicotinohydrazide (200μM-Cayman chemicals; Cat#15784) before EPO stimulation ...
-
No products found
because this supplier's products are not listed.
Qinjian Li, et al.,
bioRxiv - Biochemistry 2021
Quote:
... 2-[6-(4’-hydroxy) phenoxy-3H-xanthen-3-on-9-yl] benzoate (HPF) from Molecular Probes® and Horse radish peroxidase (HRP ...
-
No products found
because this supplier's products are not listed.
Johnson Ung, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... 20 μL of [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] (MTS) (Promega, #G3582) were added and incubated at 37°C for 3 hours ...
-
No products found
because this supplier's products are not listed.
Ludovica Iovino, et al.,
bioRxiv - Neuroscience 2022
Quote:
... were incubated for 10 min at 37 °C in the presence of 10 µM 2-amino-5,6,7,8-tetrahydro-4-(4-methoxyphenyl)-7-(naphthalen-1-yl)-5-oxo-4H-chromene-3-carbonitrile (UCPH-101; Abcam 120309, UK), a specific excitatory amino acid transporter 1 (EAAT1/Glast ...
-
No products found
because this supplier's products are not listed.
Krzysztof Mikołajczyk,
bioRxiv - Biochemistry 2024
Quote:
... and untransfected cells were cultured for 2 weeks in a complete medium containing 3 μM N-[(1R,2R)-1- (2,3-dihydro-1,4-benzodioxin-6-yl)-1-hydroxy-3-pyrrolidin-1-ylpropan-2-yl]nonanamide (Genz-123346) (Merck, Darmstadt, Germany), a glucosylceramide synthase inhibitor [26].
-
No products found
because this supplier's products are not listed.
Carlos J. Garcia, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Authentic standards of 3,7-Dihydroxy-5-cholan-24-oic Acid (chenodeoxycholic acid) and 3-Hydroxy-11-oxo-5-cholan-24-oic Acid (3-oxo-chenodeoxycholic acid) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). The N-(3α,7α,12α-trihydroxy-5β-cholan-24-oyl)-glycine (glycocholic acid) ...
-
No products found
because this supplier's products are not listed.
Blanca Salazar-Sarasua, et al.,
bioRxiv - Plant Biology 2021
Quote:
... in paraffin-embedded sections (8µm) and color was detected with 5-bromo-4-chloroindol-3-yl phosphate/nitrateblue tetrazolium (BCIP/NBT) (Roche).
-
No products found
because this supplier's products are not listed.
Katja Hartstock, et al.,
bioRxiv - Biochemistry 2022
Quote:
... 1× CutSmart buffer (50 mM potassium acetate, 20 mM Tris-acetate, 10 mM magnesium acetate,100 μg/mL BSA, pH 7.9, NEB), 20 U RiboLock ...
-
No products found
because this supplier's products are not listed.
Eva Hitz, et al.,
bioRxiv - Microbiology 2021
Quote:
... Protein lysates were separated on NuPage 5-12% Bis-Tris or 3-8% Tris-Acetate gels (Novex, Qiagen) using MES running buffer (Novex ...
-
No products found
because this supplier's products are not listed.
Fabien Thery, et al.,
bioRxiv - Cell Biology 2021
Quote:
... 3-8% Criterion XT tris- acetate gel (#3450130, Biorad) or 4-15% Criterion TGX gel (#5671083 ...
-
No products found
because this supplier's products are not listed.
Gina Partipilo, et al.,
bioRxiv - Bioengineering 2024
Quote:
... disodium;4-[3-pyridin-2-yl-6-(4- sulfonatophenyl)-1,2,4-triazin-5-yl]benzenesulfonate hydrate (Ferrozine, VWR) All media components were autoclaved or sterilized using 0.2 μm PES filters.
-
No products found
because this supplier's products are not listed.
Jonathan Pini, et al.,
bioRxiv - Cell Biology 2020
Quote:
... 20 ng/ml Interleukin-3 (IL-3, Peprotech) and 20 ng/ml IL-6 (Peprotech) ...
-
No products found
because this supplier's products are not listed.
Max Meyrath, et al.,
bioRxiv - Pharmacology and Toxicology 2024
Quote:
... 5-oxo-eicosatetraenoic acid and prostaglandin from Santa Cruz, Leukotriene and PAF from Tocris ...
-
No products found
because this supplier's products are not listed.
Ana Popovic, et al.,
bioRxiv - Microbiology 2023
Quote:
... and counter stained with saturated 5% uranyl acetate (EMS), followed by Reynold’s lead citrate (EMS) ...
-
No products found
because this supplier's products are not listed.
Yoshiki Matsuda, et al.,
bioRxiv - Neuroscience 2020
Quote:
... 341F (5’-CCTACGGGNGGCWGCAG-3’) and 805R (5’-GACTACHVGGGTATCTAATCC-3’) or (2) 341F (5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG-3’) and 806R (5’-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGGACTACHVGGGTWTCTAAT-3’) (TaKaRa Bio, Shiga, Japan). The second PCR was performed to add the index sequences for Illumina sequencing with barcode sequences ...
-
No products found
because this supplier's products are not listed.
Huan Du, et al.,
bioRxiv - Neuroscience 2021
Quote:
... targeted to mouse PGRN exon 1 (oligos with 5’-cacc gGCTCCCTGGGAGGCATCTGG-3’ and 5’-aaac CCAGATGCCTCCCAGGGAGCc-3’ or 5’-cacc gCGGACCCCGACGCAGGTAGG-3’ and 5’-aaac CCTACCTGCGTCGGGGTCCGc-3’ were ligated to pLenti-CRISPRv2 (Addgene)) or Cas9 only ...
-
No products found
because this supplier's products are not listed.
Yasuhiro Oda, et al.,
bioRxiv - Microbiology 2024
Quote:
... 20 mM acetate and 0.3% Difco Agar Noble (BD) that were held in a 60°C water bath ...
-
No products found
because this supplier's products are not listed.
Taijin Lan, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... 8-oxo-dG (R&D systems, #4354-MC-050), γ-H2AX (Cell Signaling ...
-
No products found
because this supplier's products are not listed.
Senthil T. Kumar, et al.,
bioRxiv - Neuroscience 2020
Quote:
... at 10-20 μg/mL concentration in 10 mM acetate solution (GE Healthcare) at pH 4.5 to reach a final surface ligand density of around 2000-4000 response units (RUs) ...
-
No products found
because this supplier's products are not listed.
Marco S. Kaiser, et al.,
bioRxiv - Physiology 2021
Quote:
... 3’ and 5’ adaptors (Illumina) were ligated and the resulting product was reverse transcribed to generate cDNA by PCR ...
-
No products found
because this supplier's products are not listed.
Felix Scharte, Rico Franzkoch, Michael Hensel,
bioRxiv - Microbiology 2022
Quote:
... Grids were post-stained for 30 min with 2% uranyl acetate (Roth) and 20 min with 3% lead citrate (Leica). TEM images were acquired using a Zeiss Leo 912 Omega (Zeiss ...
-
No products found
because this supplier's products are not listed.
Hae Young Ko, et al.,
bioRxiv - Cancer Biology 2022
Quote:
Mouse orthotopic xenograft models (4-5 mice per each group) were intravenously injected with 14C-acetate (3 μCi, PerkinElmer) in 200 μL saline and perfused with 4% paraformaldehyde (PFA ...
-
No products found
because this supplier's products are not listed.
Dan Xia, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Mobile phase A consisted of water with 10 mM ammonium acetate + 5 μM medronic acid (Agilent), pH 9 ...
-
No products found
because this supplier's products are not listed.
Syed-Rehan A. Hussain, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... The selected sgRNA with additional 20 bp RP-loop [5’TCTCCCTGAGCTTCAGGGAG-3’] at the 5’ end of guide RNA was custom synthesized by Genscript, cloned into plasmid pUC57 with unique restriction sites (Pcil ...
-
No products found
because this supplier's products are not listed.
Saumya Saurabh, et al.,
bioRxiv - Microbiology 2021
Quote:
... 20 µLof (3-Aminopropyl)triethoxysilane (APTES, Vector labs) was added to each well ...
-
No products found
because this supplier's products are not listed.
Tarek E. Moustafa, et al.,
bioRxiv - Bioengineering 2024
Quote:
20 µm Polystyrene beads (18329-5, Polysciences, USA) were embedded in NOA73 (NOA73 ...
-
No products found
because this supplier's products are not listed.
Miranda L. Curtiss, et al.,
bioRxiv - Immunology 2022
Quote:
50μg biotinylated NS1 protein or 50μg (4-hydroxy-3-nitrophenyl)-acetyl(15)-OVA (NP-OVA) was adsorbed to 100μg alhydrogel alum (InVivoGen) in a total of 200μL per mouse for 30 min at room temperature ...
-
No products found
because this supplier's products are not listed.
Liam K. R. Sharkey, et al.,
bioRxiv - Microbiology 2023
Quote:
... The proteins were spin concentrated to 5 mg / ml (3 kDa MWCO, vivaspin 20, Sartorius), aliquoted ...
-
No products found
because this supplier's products are not listed.
Johannes Textor, et al.,
bioRxiv - Immunology 2023
Quote:
... 20 ng/mL IL-3 (Biolegend), 50 ng/mL IL-6 (Biolegend) ...
-
No products found
because this supplier's products are not listed.
Yili Zhang, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Intracellular recordings from MNs were made with 10-20 MΩ sharp electrodes filled with 3 M potassium acetate connected to an Axoclamp 2-B amplifier (Molecular Devices). Data acquisition and analyses of resting potential ...
-
No products found
because this supplier's products are not listed.
Amy Collins, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Hydroxy-HIF-1α (Pro564) (#3434, Cell Signaling Technologies), VHL (#68547 ...
-
No products found
because this supplier's products are not listed.
Thomas Parmentier, et al.,
bioRxiv - Neuroscience 2022
Quote:
... and Neurotrophin-3 (NT-3; 20 ng/ml, Stemcell technologies) and the culture medium was changed every 3-4 days ...
-
No products found
because this supplier's products are not listed.
Yunlong Cao, et al.,
bioRxiv - Genomics 2023
Quote:
... cytosines in 87bp DNA fragment were modified with hydroxy-methylation by PCR amplification with 5-hydroxymethyl-dCTP supplement (ZYMO). The hydroxy-methylated DNA fragment was combined with equal amounts of CpG methylated pUC19 DNA (NEB ...
-
No products found
because this supplier's products are not listed.
Nicholas B. Chamberlain, Ted Hackstadt,
bioRxiv - Microbiology 2022
Quote:
... AM readings were performed by measuring fluorescence at 340/11 nm and 380/20 nm excitation and 508/20 emission every 20 seconds for 5 minutes using a Cytation 5 (BioTek). The solution was replaced with Ca2+-free Ringer’s solution containing either 2 μM thapsigargin (TG ...
-
No products found
because this supplier's products are not listed.
Chang-Bin Jing, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... or either of two different gRNAs targeting exon 3 coding sequences of human TET2 (TET2-gRNA #1: 5’-TGGAGAAAGACGTAACTTC-3’ and TET2-gRNA #2: 5’-TCTGCCCTGAGGTATGCGAT-3’) were transfected with Nucleofector (Lonza). After transduction ...
-
No products found
because this supplier's products are not listed.
Jae-Hyun Yoon, et al.,
bioRxiv - Microbiology 2020
Quote:
... supplemented with 5% (DMEM5) and 20% (DMEM20) fetal bovine serum (FBS, Corning) at 37°C for 2 days in 5% CO2 ...
-
No products found
because this supplier's products are not listed.
Nioosha Nekooie-Marnany, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... Cy-3 or Cy-5 (Jackson Immunoresearch Laboratories), and processed for DAPI or Hoechst staining to visualize cells’ nuclei before mounting in ImmuMount medium (Shandon) ...
-
No products found
because this supplier's products are not listed.
Johanna Tüshaus, et al.,
bioRxiv - Neuroscience 2020
Quote:
... conditioned media was collected and filtered through Spin-X 0.45 µM cellulose acetate centrifuge tube filter (#8163, Costar) and stored at −20°C in protein Lobind tubes (Eppendorf) until further usage ...
-
No products found
because this supplier's products are not listed.
John K. Mich, et al.,
bioRxiv - Neuroscience 2023
Quote:
... the following primers were used: (5’-GGTTTCCCGCAGAACCTGAA-3’) and (5’-CCATCGCTCGACCAGTTTAGT-3’) (Jackson Laboratories)
-
No products found
because this supplier's products are not listed.
Kamal Mandal, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... and 5 mg of C18 solid phase (3 μm, Durashell, Phenomenex). The HpHt column was sequentially washed with a series of 3 different solvents/solutions namely methanol ...
-
No products found
because this supplier's products are not listed.
Suha Naffar-Abu Amara, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... 3 and 5 and counted using a particle counter (Z1; Beckman Coulter, Inc.). Experiments were carried out in triplicate ...
-
No products found
because this supplier's products are not listed.
Jérôme Wahis, et al.,
bioRxiv - Neuroscience 2023
Quote:
... cells were washed with PBS (3 x 5 minutes) and mounted on standard microscope glass slides using DAPI-Fluoromount G (SouthernBiotech, 0100-20). Antibodies and dilutions used were as follows ...
-
No products found
because this supplier's products are not listed.
Simon Maksour, et al.,
bioRxiv - Neuroscience 2024
Quote:
... and 20 ng/mL NT-3 (Miltenyi Biotec, #130-093-973) from day 34 ...
-
No products found
because this supplier's products are not listed.
Tao Sun, et al.,
bioRxiv - Bioengineering 2021
Quote:
Male BALB/c mice (Charles River Laboratories 20-25 g, n=3) were used to evaluate the ability to interface the cervical vagus ...
-
No products found
because this supplier's products are not listed.
Aniruddha Das, et al.,
bioRxiv - Neuroscience 2022
Quote:
... A 3×5 mm2 craniotomy was drilled (Omnidrill35, World Precision Instruments) over an area covering the monocular and binocular primary visual (V1m and V1b ...
-
No products found
because this supplier's products are not listed.
Srinjoy Sil, Jef D Boeke, Liam J Holt,
bioRxiv - Cell Biology 2022
Quote:
... 5×5 large images were acquired with 20% overlap using the ND Acquisition feature NIS Elements software (Nikon) and were stitched together using the SiR-DNA channel.
-
Prepared to contain higher collagenase and caseinase activities. A dialyzed, lyophilized powder.
Cat# LS005282,
1 gm, $246.00
Ask
Katherine L. Leiby, et al.,
bioRxiv - Bioengineering 2022
Quote:
... and then inflated via the trachea with 2-3 mL of pre-warmed (37°C) enzyme solution (1 mg/mL collagenase/dispase [Roche], 3 U/mL elastase [Worthington], 20 U/mL DNase [Worthington] in DMEM). The heart and large airways were dissected away and the remaining lung tissues and enzyme solution were collected and transferred to a shaking 37°C water bath at 75 rpm for 20 min ...
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No products found
because this supplier's products are not listed.
Jingu Lee, et al.,
bioRxiv - Neuroscience 2021
Quote:
... A cerebral microinfarction was induced for 3-5 seconds by 561nm laser illumination with 60X objective lens (LUMFLN60XW, NA 1.1, Olympus) after intravenous injection of 100μl Rose Bengal (15mg/ml ...