Quote:
... membranes were stripped at 4°C in agitation using Antibody stripping buffer 1X for 10 minutes (Gene Bio-Application). Protein bands were quantified using ImageJ software ...
Anna M. Jermakowicz, et al.,bioRxiv - Cancer Biology 2023
Quote:
... Cells were rinsed with PBS and then lysed with the Western Lysis Buffer (1:10, PhosphoSolutions, Aurora, CO, USA) supplemented with protease/phosphatase inhibitor cocktail (1:100 ...
Jessica Lacoste, et al.,bioRxiv - Cell Biology 2023
Quote:
... capillary columns were pulled with a laser puller and packed in-house with 10–12 cm C18 (Reprosil-Pur 120 C18-AQ, 3 μm, Dr. Maisch HPLC GmbH) in methanol ...