-
No products found
because this supplier's products are not listed.
Yuan Weng, et al.,
bioRxiv - Cell Biology 2023
Quote:
N-[4-(7-diethylamino-4-methyl-3-coumarinyl)phenyl]maleimide (CPM, Sigma) was dissolved in DMSO at 4 mg/ml as stock solution and diluted 20 times using a buffer containing 25 mM HEPES ...
-
No products found
because this supplier's products are not listed.
Pojeong Park, et al.,
bioRxiv - Neuroscience 2020
Quote:
... 4-[(2S)-2-[(5-isoquinolinylsulfonyl)methylamino]-3-oxo-3-(4-phenyl-1-piperazinyl)propyl] phenyl isoquinolinesulfonic acid ester (KN-62; Tocris and HelloBio); D-AP5 (HelloBio) ...
-
No products found
because this supplier's products are not listed.
Nathaniel L. Hepowit, et al.,
bioRxiv - Cell Biology 2022
Quote:
... For the bulk endocytosis experiments using the 4-[6-[4-(diethylamino)phenyl]-1,3,5-hexatrien-1-yl]-1-[3-(triethylammonio)propyl]-pyridiniumbromide dye (FM 4-64, Invitrogen), the cells were prepared as previously described [41] ...
-
No products found
because this supplier's products are not listed.
Ivona Mateska, et al.,
bioRxiv - Immunology 2022
Quote:
... 4-Hydroxynonenal (4-HNE, 1:200; Abcam, # ab48506), Alexa Fluor 555 donkey anti-rabbit (1:300 ...
-
No products found
because this supplier's products are not listed.
Rachel D. Kelly, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Pdcd-4 (B-4, Santa Cruz, 1:1000), and Rabbit α-tubulin (ab15246 ...
-
No products found
because this supplier's products are not listed.
Ashwin Narayanan, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... concentrations was assessed using reduction in WST-1 (4-[3-(4-Iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]- 1,3-benzene Disulfonate) to water-soluble formazan (Roche, France). Cells were seeded in 96-well plates at 2 × 104 cells/well and treated with the different NAM concentrations at 37°C ...
-
No products found
because this supplier's products are not listed.
John Ong, et al.,
bioRxiv - Bioengineering 2024
Quote:
... 10μM of 2-(4-Morpholinyl)-8-phenyl-4 H-1-benzopyran-4-one (Ly294002: Promega, #V120A), 50ng/ml of Activin A (Stemcell Technologies ...
-
No products found
because this supplier's products are not listed.
Romain Vaineau, et al.,
bioRxiv - Immunology 2024
Quote:
... CTLA-4 (BV605, 1/40, BioLegend), PD-1 (PE-Cy5.5 ...
-
No products found
because this supplier's products are not listed.
Kevin C. Courtney, et al.,
bioRxiv - Neuroscience 2021
Quote:
... and 1,2-dioleoyl-sn-glycero-3-phospho-(1′-myo-inositol-4′,5′- bisphosphate) (PI(4, 5)P2) were obtained from Avanti Polar Lipids. HEPES was from Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Liang Wang, et al.,
bioRxiv - Cell Biology 2023
Quote:
... #4: 5’-CCGGTTTAGCTGAAGATTCAA-3’ (SI00443779, Qiagen), GTPBP10 siRNA 5’-TTGCGTGTTGTTCAGAAAGTA-3’ (SI04308647 ...
-
No products found
because this supplier's products are not listed.
Calida Mrabti, et al.,
bioRxiv - Physiology 2024
Quote:
... NEL105001EA).Antibodies: mouse OCT-3/4 (C-10) (1:3000, Cell Signaling, 13969); mouse β-Actin (AC-74 ...
-
No products found
because this supplier's products are not listed.
Harikumar R. Suma, Pierre Stallforth,
bioRxiv - Microbiology 2024
Quote:
... The chromatic mutants were mixed in different ratios (1:1, 1:3, 3:1) and the mixtures were fixed using 4% paraformaldehyde (Sigma−Aldrich/Merck, Germany) for 4 h at 4°C.
-
No products found
because this supplier's products are not listed.
Alexander Stanton, et al.,
bioRxiv - Genetics 2024
Quote:
... and 1:300 OCT 3/4 primary antibody (BD Biosciences 611202) at 4C for 1 hour ...
-
No products found
because this supplier's products are not listed.
Angela Castoldi, et al.,
bioRxiv - Immunology 2020
Quote:
... or 20 ng ml−1 IL-4 (Peprotech; MI(L-4)) ...
-
No products found
because this supplier's products are not listed.
Takahiro Hiono, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... β1-4 galactosidase (Agilent Technologies), or double digestion with Sialidase A and β-galactosidase ...
-
No products found
because this supplier's products are not listed.
Michelle Reed, et al.,
bioRxiv - Neuroscience 2022
Quote:
... ARL13B (1 hour 4% PFA,1:350, ProteinTech); IFT57 (10 min 4% PFA ...
-
No products found
because this supplier's products are not listed.
Ana Monfort-Vengut, et al.,
bioRxiv - Cancer Biology 2024
Quote:
WNK1 depletion by CRISPR-Cas9 editing was done by using two independent gRNAs targeting Exon-1 (5’-CGCCGACGCTGTGACCGGC-3’) and Exon-4 (5’-ACTTACACTGGTCACGCGA-3’) cloned into pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W backbone vector (Addgene #67974). TET-ON-Cas9 expressing cells were infected and BFP-positive cell FACS sorted ...
-
No products found
because this supplier's products are not listed.
José A. Noriega-Prieto, et al.,
bioRxiv - Neuroscience 2020
Quote:
... or the IGF-1R antagonist 7-[cis-3-(1-azetidinylmethyl)cyclobutyl]-5-[3-(phenylmethoxy)phenyl]-7H-pyrrolo[2,3-]pyrimidin-4-amine (NVP-AEW 541, 40 μM; Cayman Chemicals) plus IGF-1 were infused into the IL ...
-
No products found
because this supplier's products are not listed.
Dounia Dems, et al.,
bioRxiv - Bioengineering 2020
Quote:
... Fmoc-(4-amino)benzoic acid and Fmoc-(4-aminomethyl)benzoic acid were purchased from VWR and Chem-Impex International Inc. ...
-
No products found
because this supplier's products are not listed.
Michaela Kreitmeier, et al.,
bioRxiv - Evolutionary Biology 2021
Quote:
... and 1×Buffer 4 (NEB). The reaction was stopped (6 mM EGTA ...
-
No products found
because this supplier's products are not listed.
Xi Cheng, et al.,
bioRxiv - Neuroscience 2024
Quote:
... followed by 4% PFA (1:4 diluted with 1× PBS from Paraformaldehyde 16% Aqueous Solution, Electron Microscopy Sciences) to pre-fix the brain ...
-
No products found
because this supplier's products are not listed.
Pratim Chowdhury, et al.,
bioRxiv - Cell Biology 2024
Quote:
... were diluted 1:5 in 10% polyvinyl alcohol (Mowiol 4-88, 17951, Polysciences Inc.) in MilliQ water and spun-coat onto plasma-cleaned coverslips (#1.5H ...
-
No products found
because this supplier's products are not listed.
Mingji Li, et al.,
bioRxiv - Synthetic Biology 2021
Quote:
Supernatant or periplasmic fractions were diluted 3:1 in 4× Laemmli sample buffer (Bio-Rad) and were boiled at 100 °C for 10 min ...
-
No products found
because this supplier's products are not listed.
Lea Zillich, et al.,
bioRxiv - Neuroscience 2024
Quote:
... OCT3/4 (1:500, R&D Systems), SMA (1:400 ...
-
No products found
because this supplier's products are not listed.
Anastasia Selyutina, et al.,
bioRxiv - Microbiology 2020
Quote:
... supplemented with 5 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (Corning, Corning, NY, USA), 50 μg/ml penicillin/streptomycin (Corning) ...
-
No products found
because this supplier's products are not listed.
Mengmeng Jin, et al.,
bioRxiv - Neuroscience 2024
Quote:
... in a feeder free fashion and passaged every 4∼5 days at a 1:4∼1:6 ratio using ReLeSR (Stemcell technologies) as described previously 88,95 ...
-
No products found
because this supplier's products are not listed.
Christophe Royer, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... 3 to 4 week old CD-1 females (Charles River UK) were injected intraperitoneally with 5 IU of PMSG (Intervet ...
-
No products found
because this supplier's products are not listed.
Shiri Levy, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Oct 4 (Novus Biologicals, NB11- 1:150), P16 (Invitrogen MA5-14260 ...
-
No products found
because this supplier's products are not listed.
Jorge Diego Martin-Rufino, et al.,
bioRxiv - Genomics 2024
Quote:
... 4°C for 1:30h (Beckman Coulter SW32Ti). The pellet was ultimately resuspended with StemSpanTM SFEM-II media (Stemcell Technologies ...
-
No products found
because this supplier's products are not listed.
Justin E. Silpe, et al.,
bioRxiv - Microbiology 2021
Quote:
... 40 μg mL−1 5-Bromo-4-Chloro-3-Indolyl-beta-D-Galactosidase (X-gal, Takara Bio), and 500 μM isopropyl beta-D-1-thiogalactopyranoside (IPTG ...
-
No products found
because this supplier's products are not listed.
Bui Ba Han, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... and 4 μg ml-1 puromycin (InvivoGen), and grown at 37 °C in an atmosphere containing 5% CO2 ...
-
No products found
because this supplier's products are not listed.
Jonas Gockel, et al.,
bioRxiv - Genomics 2024
Quote:
... 4 °C with acceleration and deceleration set to 1 (Eppendorf 5910 Ri, Rotor S-4×400).
-
No products found
because this supplier's products are not listed.
Christine S. Hopp, et al.,
bioRxiv - Immunology 2020
Quote:
Columns of 1 ml Protein G sepharose 4 fast flow (GE Healthcare) were prepared and equilibrated with 5 volumes of binding buffer (0.02 M sodium phosphate ...
-
No products found
because this supplier's products are not listed.
Yiqiong Liu, et al.,
bioRxiv - Neuroscience 2024
Quote:
... for 1 day at 4 °C: rabbit anti-cFos (1:2000, Synaptic systems, #226003), chicken anti-GFP (1:2000 ...
-
No products found
because this supplier's products are not listed.
Luke F. Nunnelly, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... and St18 (1:1000) were incubated in 5% NDS/PBS overnight at 4°C and secondary antibodies (Jackson Immunoresearch, 1:1000) diluted in 0.01% Triton X-100/PBS for 2 hours at room temperature ...
-
No products found
because this supplier's products are not listed.
Elvin Wagenblast, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Between 1×10^3 – 5×10^4 cells were re-suspended in 20μl Buffer P3 (Lonza) per reaction and quickly added to the Eppendorf tube containing the CRISPR/Cas9 gRNA RNP complex ...
-
No products found
because this supplier's products are not listed.
Da-Qiao Ding, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... Fluorescently labeled RNA was obtained by replacing 1/4 volume of CTP with Cyanine 3-CTP (Cy3-CTP) or Cyanine 5-CTP (Cy5-CTP) (PerkinElmer NEL581001EA) in the in vitro transcription reaction.
-
No products found
because this supplier's products are not listed.
Zhiyuan Hu, et al.,
bioRxiv - Developmental Biology 2024
Quote:
... rabbit anti-Elavl3/4 antibody 1:200 (GTX128365, GeneTex), and chicken anti-GFP 1:200 (ab13970 ...
-
No products found
because this supplier's products are not listed.
Helen Barbas, et al.,
bioRxiv - Neuroscience 2024
Quote:
... and preblocked for 1 h at 4°C in 5% normal goat serum (NGS; Vector Laboratories), 5% bovine serum albumin (BSA ...
-
No products found
because this supplier's products are not listed.
Agnieszka Lukaszewicz, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... libraries from amplicons 1–4 were mixed with 5% PhiX DNA control library (Illumina) and sequenced ...
-
No products found
because this supplier's products are not listed.
Wenjian Lv, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Cells were passaged (1:12) every 3-4 days using StemMACS Passaging Solution XF (Miltenyi Biotec) and Y-27632 dihydrochloride (Santa Cruz ...
-
No products found
because this supplier's products are not listed.
Maria D.H. Alcorn, et al.,
bioRxiv - Microbiology 2024
Quote:
... Female 4-week-old CD-1 mice were purchased commercially (Jackson Laboratories). Virus inoculations were performed under anesthesia that was induced and maintained with isoflurane ...
-
No products found
because this supplier's products are not listed.
Rebecca E. Wagner, et al.,
bioRxiv - Cell Biology 2024
Quote:
... RFP (1:1000, Rockland 600-4-1-379), KI67 (1:400 ...
-
No products found
because this supplier's products are not listed.
Sadie P. Antine, et al.,
bioRxiv - Microbiology 2023
Quote:
... phage 1/4 Gad1 was used and synthesized by Genscript. Gad1 and related homologs were cloned into the pSG-thrC-Phspank vector40 and transformed to DH5α competent cells ...
-
No products found
because this supplier's products are not listed.
Hannah Crane, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... Wells were incubated with 160□l of primary antibody diluted in 3% BSA for 1 hour at room temperature or overnight at 4°C as follows: Senataxin (1:1000 dilution, Bethyl, A301 – 104A or 105A), USP11 (1:500 dilution ...
-
No products found
because this supplier's products are not listed.
Jiefu Wang, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Tissues were fixed with 4% PFA for 1 h prior to vibratome (Leica) sectioning at 100 μm ...
-
No products found
because this supplier's products are not listed.
Steven Tau, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... Plates were imaged every 3-4 d on a Cytation 5 (BioTek) with 4x magnification and phase contrast ...
-
No products found
because this supplier's products are not listed.
Leticia Peris, et al.,
bioRxiv - Neuroscience 2021
Quote:
... at 4°C overnight and 1 h with appropriate secondary antibodies (LI-COR Biosciences). Image acquisition was performed with an Odyssey infrared imaging system (LI-COR Biosciences ...
-
No products found
because this supplier's products are not listed.
Lara Taniguchi, et al.,
bioRxiv - Neuroscience 2024
Quote:
... Borosilicate glass pipettes (3–4 MΩ, Molecular Devices) filled with internal solution (295–305 mOsm ...
-
No products found
because this supplier's products are not listed.
Walden Li, Ryan M. Wyllie, Paul A. Jensen,
bioRxiv - Microbiology 2020
Quote:
... Strains carrying the pLacZ plasmid appear blue when plated on 0.008% X-gal (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside) (Zymo Research, CA, USA). X-gal concentrations above 0.008% appear to inhibit growth of S ...