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Citations for Takara Bio :
1 - 15 of 15 citations for pcDNA5 FRT since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... with 1.6 µg WT or mutant pcDNA5/FRT/TO Opti-hERG and 0.4 µg peGFP (Clontech, France) for fluorescence-based cell selection in microscopy24 ...
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bioRxiv - Cell Biology 2024Quote: ... France) and was used to clone mitoTRPV1 (pcDNA5/FRT-mitoTRPV1) using the Infusion Cloning method (Takara Bio). First ...
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bioRxiv - Cancer Biology 2021Quote: ... whereas a TOPBP1 fragment corresponding to residues 2-1523 was amplified from pCDNA5-FRT/TO-LacR-FLAG-TopBP1 and cloned into pGBKT7 (Clontech/Takara) to create a fusion with the GAL4 DNA binding domain using the NdeI and XmaI sites ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA encoding HsPINK1 was inserted into the BamHI site of pcDNA5/FRT/TO CMVd3 vector (pcDNA5d3, see (17)) using the In-Fusion HD Cloning Kit (Takara). For stable expression ...
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bioRxiv - Cell Biology 2024Quote: ... was inserted into pcDNA5-FRT/TO to generate pcDNA/TO/Dyn1-K44A-GFP using the In-Fusion cloning kit (Takara), and the following primers ...
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bioRxiv - Cancer Biology 2021Quote: ... whereas a TOPBP1 fragment corresponding to residues 2-1523 was amplified from pCDNA5-FRT/TO-LacR-FLAG-TopBP1 and cloned into pGBKT7 (Clontech/Takara) to create a fusion with the GAL4 DNA binding domain using the NdeI and XmaI sites ...
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bioRxiv - Cell Biology 2022Quote: ... The PCR products were subcloned into pcDNA5 FRT/TO HA FLAG Hu Jaw1 or pcDNA5 FRT/TO Hu Jaw1 (digested with HpaI/XhoI) using an In-Fusion HD Cloning Kit (#Z9648N; TaKaRa, Kusatsu, Japan), resulting in pcDNA5 FRT/TO HA FLAG Hu Jaw1 opsin ...
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bioRxiv - Cell Biology 2022Quote: ... The PCR products were subcloned into the pcDNA5 FRT/TO HA FLAG Ms Jaw1 (digested with HpaI/BamHI) using an In-Fusion HD Cloning Kit (#Z9648N; TaKaRa, Kusatsu, Japan). Similarly ...
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bioRxiv - Cell Biology 2022Quote: ... and the DNA fragments were ligated to the pcDNA5 FRT/TO HA FLAG vector (digested with the same enzymes) using a DNA Ligation Kit (#6023; TaKaRa, Kusatsu, Japan), resulting in pcDNA5 FRT/TO HA FLAG Hu sec11a and pcDNA5 FRT/TO HA FLAG Hu sec11c ...
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bioRxiv - Cell Biology 2022Quote: ... The PCR products were subcloned into the pcDNA5 FRT/TO HA FLAG Ms Jaw1 (digested with AfeI/BamHI) using an In-Fusion HD Cloning Kit (#Z9648N; TaKaRa, Kusatsu, Japan) to swap the luminal region of Jaw1 to that of KASH proteins ...
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bioRxiv - Cell Biology 2022Quote: ... and the DNA fragments were ligated into the pcDNA5 FRT/TO HA FLAG vector (digested with same enzymes) using a DNA Ligation Kit (#6023; TaKaRa, Kusatsu, Japan), resulting in pcDNA5 FRT/TO HA FLAG Ms Jaw1 PA and pcDNA5 FRT/TO HA FLAG Ms Jaw1 NIDR PA ...
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bioRxiv - Cell Biology 2022Quote: ... The pcDNA5 FRT/TO HA FLAG Hu AASS was then digested with BamHI/ApaI and the DNA fragment was ligated into the pcDNA5 FRT/TO vector using a DNA Ligation Kit (#6023; TaKaRa, Kusatsu, Japan), resulting in pcDNA5 FRT/TO Hu AASS ...
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bioRxiv - Neuroscience 2020Quote: ... a series of FRT-FP plasmids were constructed (Fig. S1) using the mammalian expression backbone pCMV-N1 (Clontech). FRT-F13 ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by the E.coli xthA gene (coding Exonuclease III) cloned after the second FRT site via in-fusion cloning (Takara). Subsequently ...
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bioRxiv - Developmental Biology 2021Quote: ... was constructed by inserting a gBlock encoding sfGFP followed by an SV40 polyA sequence and a FRT-flanked ampicillin cassette in place of EGFP in the pEGFP-C1 plasmid (Clontech). For kcne4 and ndufa4l2a BACs ...