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Citations for Takara Bio :
1 - 41 of 41 citations for pNFkB DD tdTomato since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: hCEC were transduced with pHAGE-DD-KNL1Mut-dCas9 and a sgRNA vectors and DD-KNL1Mut-dCas9 was stabilized with 100 nM Shield-1 (Takara, #632189) for 9 days ...
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bioRxiv - Neuroscience 2020Quote: A TDTOMATO fluorophore (Clontech) was subcloned into a pCAG backbone to generate the pCAG-TDTOMATO plasmid ...
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bioRxiv - Neuroscience 2020Quote: ... and tdTomato (Takara, 632496) were used at 1:5,000 ...
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bioRxiv - Cell Biology 2023Quote: ... tdTomato (Takara Bio, 632496), Dystrophin (DSHB ...
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bioRxiv - Cell Biology 2020Quote: ... Shield-1 ligand for stabilization of DD-tagged proteins was purchased from Takara Bio (Cat # 632189 ...
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bioRxiv - Cell Biology 2021Quote: ... Lentivirus (pLVX myristoylated tdTomato, Clontech) transduction of keratinocytes utilized virus obtained from the Northwestern University SBDRC ...
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bioRxiv - Cell Biology 2024Quote: ... anti-tdTomato (Takara, Cat.# 632496), anti-K14 (Biolegend ...
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bioRxiv - Developmental Biology 2024Quote: ... anti-tdTomato (Takara, Cat.# 632496), anti-K14 (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... and then the expression construction of CMV-tdTomato was amplified from pCMV-tdTomato vector (Clontech, 632534) to inserted downstream of HA-R by GeneArt Gibson Assembly (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: ... A plasmid encoding tdTomato was obtained from Takara Bio (#632533 ...
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bioRxiv - Neuroscience 2020Quote: ... pN1-EGFP or pC1-tdTomato (Clontech; Mountain View, CA) were mixed with AMPAR-expressing plasmids at a base pair ratio of 1:7 ...
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bioRxiv - Microbiology 2020Quote: ... pEGFP-N1 and pCMV-TdTomato were obtained from Clontech.
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bioRxiv - Cell Biology 2023Quote: HeLa cells and hippocampal neurons were transfected with plasmids encoding FM4 recombinant receptors for 15 h and then treated with 2 μM DD-Solubilizer (TakaraBio/Clontech) to induce the release of the receptors from the ER into the secretory trafficking ...
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bioRxiv - Microbiology 2020Quote: ... pEGFP-N1 and pCMV-TdTomato were commercially purchased from Clontech. Codon-optimized SARS-CoV-2 S was a kind gift from Dr ...
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bioRxiv - Cell Biology 2024Quote: ... 1:1000 anti-tdtomato rabbit primary antibody (#632496 from Takara), 1:1000 anti β-tubulin mouse primary antibody ...
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bioRxiv - Genomics 2023Quote: ... and tdTomato fluorescent protein using in-Fusion cloning (Takara Bio). The 5-plasmid system includes a packaging vector (pHAGE-H2B-NanoLuc-T2A-tdTomato) ...
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bioRxiv - Immunology 2021Quote: ... Both guides were cloned into pGuide-it-tdTomato (TaKaRa Bio Europe) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2020Quote: A lentiviral plasmid encoding a transcriptional reporter for CREB activity was generated by PCR amplification of a 2xCRE promoter-driven GFP N-terminally tagged with the ProteoTuner destabilization domain (CRE-DD-GFP, Takara Bio, Cat #631085) and Gibson cloning into the FUGW lenti-vector backbone (Addgene ...
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bioRxiv - Neuroscience 2022Quote: ... we used primary polyclonal rabbit anti-tdTomato from Takara (Cat no. 632496) and secondary anti-rabbit goat Cy3 (Cat no ...
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bioRxiv - Developmental Biology 2023Quote: K14-HA-Fzd6-tdTomato constructs were generated by using In-Fusion (Takara) cloning to first add the tdTomato tag from pBluescript (Stratagene ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-dsRed (cross-reacts with TdTomato, 1:1,000, Clontech, Cat#632496), mouse anti-FAT36,7 (1:200) ...
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bioRxiv - Neuroscience 2024Quote: ... QUAS-tdTomato-NLS plasmid was cloned using Infusion HD Cloning Kit (Takara) and sent to Genetivision Corporation for injection ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were transfected using a lentiviral vector containing tdTomato (rLV. EF1. tdTomato9, Clontech), according to the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit Living Colors anti-DsRed (used for mCherry, tdTomato; 1:1000; Takara Bio, 632496); goat anti-RFP (used for mCherry ...
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bioRxiv - Cell Biology 2024Quote: ... (1:1000 anti GAPDH) (1:1000 anti-TdTomato rabbit primary antibody #632496 from Takara).
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Constitutive and conditional epitope-tagging of endogenous G protein coupled receptors in DrosophilabioRxiv - Neuroscience 2023Quote: Myristoylated TdTomato (myr::TdTom) was labeled with 1:500 rabbit anti-DsRed (Takara Cat# 632496, RRID:AB_10013483)
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bioRxiv - Neuroscience 2023Quote: ... Native tdTomato fluorescence destroyed by combined ISH protocol was recovered by rabbit anti-DsRed (1:1000, Takara Bio Clontech # 632496 ...
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bioRxiv - Developmental Biology 2023Quote: ... The amplicon was then inserted into the pUASz-tdTomato vector using the In-fusion cloning technique (Takara) following digestion with AgeI ...
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bioRxiv - Cell Biology 2022Quote: ... 5um sections from paraffin blocks were used for immunofluorescence staining with the following primary antibodies: tdTomato (dsRed Mouse: Takara Biosystems 632392 ...
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bioRxiv - Genomics 2024Quote: ... The cells were infected with prepackaged lentiviral particles (constitutive reporter vector expressing tdTomato fluorescent protein gene driven by EF1a promoter (Takara) at MOI of 20 (Stock ...
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bioRxiv - Neuroscience 2024Quote: ... PCR-amplified and gel-purified Homology Box A and iCre-P2A-TdTomato were inserted into iTV vector by In-Fusion (Clontech) and transformed into DH10B bacteria ...
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bioRxiv - Bioengineering 2024Quote: ... The fibroblasts were fluorescently labeled by transduction with lentiviral particles for fluorescent whole-cell labeling with tdTomato expression (Takara 0037VCT). A solution of polybrene and lentivirus at 20 multiplicities of infection (MOI ...
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bioRxiv - Synthetic Biology 2021Quote: ... pCR-U6-gRNA-TRE3G-TdTomato and -Nluc were generated by replacing the miniCMV promoter of lenti sgRNA(MS2)_puro-miniCMV-TdTomato/Nluc with the TRE3G promoter from pTRE3G (Takara, #631173) using Gibson Assembly and inserting into pCR Blunt II-TOPO ...
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bioRxiv - Neuroscience 2024Quote: ... pCAG-EGFP-WPRE and pCAG-floxedSTOP-tdTomato-WPRE (Egawa and Yawo, 2019) using In-Fusion® Snap Assembly Master Mix (Takara) or NEBuilder® HiFi DNA Assembly Master Mix (NEB) ...
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bioRxiv - Cell Biology 2022Quote: ... 5um sections from paraffin blocks were used for immunofluorescence staining with the following primary antibodies: tdTomato (dsRed Mouse: Takara Biosystems 632392, Rabbit: Takara Biosystems 632496), GFP (Abcam 6673) ...
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bioRxiv - Neuroscience 2023Quote: ... Native tdTomato fluorescence destroyed by combined ISH protocol was recovered by rabbit anti-DsRed (1:1000, Takara Bio Clontech # 632496, RRID: AB_10013483) antibody and switched to the green channel using an Alexa Fluor 488 secondary ...
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bioRxiv - Cell Biology 2023Quote: The cDNA of sorted tdTomato positive hair cells was synthesized using PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time, RR047A, Takara Bio). Synthesized cDNA was subsequently mixed with qPCR primers and TB Green Premix Ex Taq (Tli RNase H Plus ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were directed against IBA1 (rabbit-anti-iba1 WAKO chemicals 019-19741 1:1000) and tdTomato (mouse-anti-dsRed Takara 632392 1:1000). Secondary antibodies were goat anti-rabbit or anti-mouse conjugated with Alexa dyes 488 and 568 (1:1000) ...
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bioRxiv - Bioengineering 2023Quote: ... T-2A-H2B-tdTomato was generated by replacing the EGFP fragment with H2B-tdTomato using the In-Fusion® HD Cloning Kit (Catalog #639648, Takara, Kusatsu, Japan), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... the PLIN2 cDNA was inserted on the 5’ end of pME-tdTOMATO using In-Fusion HD Cloning Plus (Takara, Mountain View, USA, Catalog #638920). Gateway cloning using the Gateway LR Clonase Enzyme mix (Thermo Fisher ...
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bioRxiv - Developmental Biology 2021Quote: ... Sections were blocked in 5% goat serum then incubated with primary antibody at 4°C overnight (anti-dsRed for tdTomato (632496, 1:1000; Takara Bio, Mountain View, CA, USA), anti-SP7 (ab22552 ...