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Citations for Takara Bio :
1 - 24 of 24 citations for pET28a DsRed2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... and the plasmid encoding DsRed2 (Clontech), cloned into a pCAX vector ...
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bioRxiv - Neuroscience 2023Quote: ... and rabbit anti-dsRed2 (1:1000, Clontech). The following secondary antibodies were used ...
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bioRxiv - Neuroscience 2022Quote: ... The 86 bp insert of Mito-DsRed2 (Clontech) was subcloned at the NheI and BsrGI sites of the pCL6EGwo vector using standard recombinant DNA technology (pCL6-Mito-DsRed2) ...
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bioRxiv - Microbiology 2023Quote: ... pET28a(+) (Takara Bio Inc.) or pGEX6P1 (Cytiva ...
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bioRxiv - Cell Biology 2020Quote: The mitochondrial targeting sequence fused with the 5’-end of DsRed2 (MTS-DsRed2) was digested from the pDsRed2-Mito vector (Clontech Laboratories, Inc., Palo Alto, CA, USA) with restriction enzymes and inserted into the pMXs-puro retroviral vector (Cell Biolabs ...
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bioRxiv - Neuroscience 2020Quote: ... we replaced the EGFP fluorophore from pMH4-hsyn-ER-EGFP with DsRed2 (Clontech). For testing expression of MyoVa-mEmerald ...
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bioRxiv - Microbiology 2021Quote: ... pET28a (TAKARA BIO, Clontech Laboratories, CA, USA) to generate pET28a:SufR ...
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bioRxiv - Microbiology 2021Quote: ... pET28a (TAKARA BIO, Clontech Laboratories, CA, USA) to generate pET28a:SufR ...
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bioRxiv - Immunology 2023Quote: ... The human J chain was replaced with the DsRed2 gene of pDsRedN1 (Takara Bio, Shiga, Japan). The pIgR gene was inserted into the pEF-Myc-His vector (ThermoFisher Scientific ...
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bioRxiv - Immunology 2020Quote: The pIRES-DsRed2 and pDsRed2-C1 vectors used to express various human aquaporin constructs were from Clontech. The human pCMV6-AC-AQP9-GFP expression vector was from Origene ...
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bioRxiv - Cell Biology 2023Quote: pET28a-Halotag-CNA35 was created using InFusion HD Assembly (Takara) by replacement of the eGFP coding sequence within pET28a-eGFP-CNA3546 (Addgene ...
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bioRxiv - Microbiology 2021Quote: abTse3-His was cloned into vector pET28a by In-Fusion (Takara Bio). E ...
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bioRxiv - Molecular Biology 2021Quote: ... a 659-bp DNA fragment of DsRed2 was amplified from 1 ng of pDsRed2-N1 plasmid (Clontech, Mountain View, CA, USA) with primers (5′-TAATACGACTCACTATAGGGCGTGCACTCGTACACTGAGG-3′ and 5′-TAATACGACTCACTATAGGGTCATCACCGAGTTCATGCG-3′) ...
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bioRxiv - Bioengineering 2022Quote: The transfer vector pABpaR2pX was constructed by inserting a DsRed2 reporter gene driven by pag promoter(46) (p-pag) into the EcoRV restriction enzyme site of pBacPAK8 (Clontech Laboratories, Inc.). cDNAs encoding HA7 and NA9 were synthesized by GenScript ...
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bioRxiv - Biochemistry 2022Quote: ... coli and cloned into pET28a using In-Fusion cloning (Takara Bio Europe, France).
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bioRxiv - Biochemistry 2020Quote: ... The product was cloned into pET28a plasmid using In-Fusion PCR Cloning Kit (Clontech), with an N-terminal hexahistidine (His6 ...
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bioRxiv - Molecular Biology 2024Quote: ... Genes and the pET28a vector were amplified using the PrimeSTAR GXL DNA Polymerase (Takara). The NEBuilder® HiFi DNA Assembly Master Mix (NEB ...
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bioRxiv - Bioengineering 2023Quote: ... The linearized pET28a vector and mfl fragment were ligated using the In-Fusion HD Cloning Kit (Clontech, Takara, Japan), and the ligated product was then transformed into E ...
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bioRxiv - Bioengineering 2023Quote: ... The linearized pET28a vector and mfl fragment were ligated using the In-Fusion HD Cloning Kit (Clontech, Takara, Japan), and the ligated product was then transformed into E ...
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bioRxiv - Biochemistry 2020Quote: ... The PCR product was cloned into pET28a digested with restriction enzymes NotI and NdeI using InFusion HD Cloning (Takara Bio). Both cloning strategies introduce a hexahistidine tag followed by a thrombin cleavage sequence ...
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bioRxiv - Molecular Biology 2020Quote: ... was amplified using specific primers (forward: 5’-AAGGAGATATACATATGCTCTCCGAAATGGTGGAAGAAG-3’; reverse:5’-GTGCGGCCGCAAGCTTTTATTTCTTTTTGTTGGTGGTCTG-3’) and cloned into pET28a using an InFusion HD Cloning Kit (Takara Bio USA) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... was amplified using the hetL pET28 fw and hetL pET28 rv infusion primers and cloned into the XhoI and NcoI restriction sites of the pET28a expression plasmid using the In-Fusion technology (Takara In-Fusion® HD Cloning kit).
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bioRxiv - Molecular Biology 2020Quote: A codon-optimized synthetic gene corresponding to the full length nucleocapsid protein (Thermo GeneArt, Regensburg, Germany) was cloned into a modified pET28a vector using the In-Fusion cloning kit (Takara Bio Saint-Germain-en-Laye, France). The resulting plasmid encoded for an N-terminal His6 tag followed by thrombin and tobacco etch virus cleavage sequences upstream of the full-length nucleocapsid protein sequence ...
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bioRxiv - Immunology 2023Quote: ... were generated by gene synthesis (Azenta Life Sciences, Burlington, MA) and cloned into NdeI-XhoI-digested pET28a by In-Fusion cloning (Takara Bio USA, Inc., San Jose, CA), according to the manufacturer’s instructions ...