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Citations for Takara Bio :
1 - 46 of 46 citations for 18 0 PE square since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... and oligo(T)18 primers (Takara). Relative transcript amounts were measured by SYBR-green quantitative PCR (qPCR ...
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ARL3 Mediates BBSome Ciliary Turnover by Promoting Its Outward Diffusion through the Transition ZonebioRxiv - Cell Biology 2021Quote: ... and oligo(T)18 primers (Takara). ARL3 cDNA and cDNAs encoding the six BBS proteins BBS1 ...
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bioRxiv - Plant Biology 2020Quote: ... total RNA isolated from Col-0 plants (RNAiso Plus; Takara) was reverse transcribed (iScript™ cDNA Synthesis Kit ...
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bioRxiv - Molecular Biology 2023Quote: ... 18 µl of Stellar (Takara Bio Europe SAS 636766) chemically competent bacteria were transformed using 2 µl of the ligation reaction ...
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bioRxiv - Cancer Biology 2023Quote: Cells were treated with 0-500 ng/mL doxycycline (Takara Bio) 48 h prior sample collection ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with 0-500 ng/mL doxycycline (Takara Bio) and imaged in an Incucyte Live-Cell Analysis system (Sartorius ...
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bioRxiv - Neuroscience 2023Quote: ... DCX: doublecortin (C-18) goat polyclonal IgG (1:200, Takara Bio, Cat# 632496). NeuN ...
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TOR Inhibition Enhances Autophagic Flux and Immune Response in Tomato Plants Against PSTVd InfectionbioRxiv - Plant Biology 2024Quote: ... (dT)18 and random primers (PerfectReal Time, Takara Bio Inc., Otsu, Shiga, Japan) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... approximately 0.6 million HEK239T cells were transfected with 1 μg pcLdGITRD-3-κB vector in each well of a 12-well culture dish and treated with varying concentrations (0, 0.5, 1.0, 2.5, 4.0 and to 5.0 μM) of Shield1 (#632189, Takara Clontech). After 48 h of transfection ...
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bioRxiv - Plant Biology 2023Quote: ... with 150-bp paired-end (PE) reads by Takara Bio ...
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bioRxiv - Microbiology 2020Quote: ... approximately 0.6 million HEK239T cells were transfected with 1 μg pcLdGITRD-3-κB vector in each well of a 12-well culture dish and treated with varying concentrations (0, 0.5, 1.0, 2.5, 4.0 and to 5.0 μM) of Shield1 (#632189, Takara Clontech). After 48 h of transfection ...
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bioRxiv - Cell Biology 2021Quote: ... 3 h and 0 h before extracting total RNA by MiniBEST Universal RNA Extraction Kit (Takara). The abundances of the interest genes were detected measured in each time point by real-time quantitative PCR (qPCR ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was extracted from Col-0 and mutant seedlings using an RNAiso Plus kit (Takara). Complementary DNAs (cDNA ...
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bioRxiv - Molecular Biology 2022Quote: ... a DNA fragment of AT1G13390 was amplified from Col-0 genomic DNA using CloneAmp HiFi PCR Premix (Clontech) with primers listed in Table S7 ...
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bioRxiv - Genomics 2022Quote: ... and post-capture PCR amplification was performed for 16-18 cycles using PrimeSTAR GXL Polymerase (Takara) at an annealing temperature of 60°C.
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bioRxiv - Physiology 2023Quote: ... Slides were incubated for 18 hours at room temperature in anti-dsRed antibody (TaKaRa, 1:2000) in 0.1M PB containing 0.3% Triton X-100 and 1% donkey serum ...
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bioRxiv - Neuroscience 2022Quote: ... Target sequences were amplified by 18–30 cycles of PCR using Ex-Taq™ (Takara Bio). For semiquantitative PCR ...
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bioRxiv - Molecular Biology 2022Quote: ... was used for oligo(dT)18-primed cDNA synthesis according to the reverse transcription protocol (TaKaRa). The resulting cDNA was subjected to relative quantitative PCR using the SYBR Premix Ex Taq kit (TaKaRa ...
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bioRxiv - Plant Biology 2020Quote: ... the promoter region of GELP38pro was PCR-amplified from Col-0 genomic DNA and cloned into linearized p1R4-ML:XVE (Siligato et al., 2016) with KpnI enzyme by Infusion (Takara) cloning to produce the inducible GELP38proXVE promoter clone ...
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bioRxiv - Cell Biology 2021Quote: ... pLL5.0-EGFP-HRasC20 was generated by insertion of EGFP-HRasC20 fragment which was amplified from pEGFP-F (#6074-1; Clontech) by PCR into pLL5.0 vector ...
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bioRxiv - Molecular Biology 2022Quote: ... Aliquots of 2.0 ug of the total RNA were collected for cDNA synthesis by using Clontech Oligo dT cDNA synthesis kit (Takara Bio USA ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted from pre-metastatic (0 hrs APF) and post-metastatic (24 hrs APF) Scrib knockdown pupae using TRI reagent (TAKARA) as per manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... with 7.0 µg of pLVX-TetOne-Puro-hMDA5 vector in 600 µl sterile water with Lenti-X Packaging Single Shots (Takara). 16 h post-transfection ...
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bioRxiv - Developmental Biology 2023Quote: ... 18-20 μg of indicated plasmids were transfected in HEK293T cells using the CalPhos Mammalian Transfection Kit (Takara). After 48h ...
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bioRxiv - Developmental Biology 2021Quote: A library scale transformation was performed on each of Dmef2-HIS + 22-Twist and tinman-HIS + 22-Twist strains using a 0-6 hr Drosophila embryonic library (a gift of L. Pick) according to the manufacturer’s instructions (Clontech PT3024-1). Transformations were plated on 150mm plates containing 12.5mM 3-AT ...
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bioRxiv - Biochemistry 2023Quote: ... The fusion between the core of the AGA1 promoter (-150 to 0) and the STL1 upstream activation sequence (-800 to -163) was generated by In-Fusion cloning (Takara Bio) into a plasmid containing the 24xPP7 stem loops ...
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bioRxiv - Genomics 2022Quote: The genomic DNA of Mir-0 accession (ID 8337) was used as a template (2 ng) for amplification using PrimeSTAR® GXL DNA Polymerase (TaKaRa), in a 40-µl PCR reaction with 0.3 µM primers OP009 and OP010 ...
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bioRxiv - Plant Biology 2022Quote: ... 1 μg total RNA was used for cDNA synthesis by oligo(dT)18 primer according to the manufacturer’s protocol (Takara). The resulting cDNA was subjected to relative quantitative PCR using the ChamQ™ SYBR qPCR Master Mix (Vazyme Biotech Co.,Ltd ...
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bioRxiv - Genomics 2022Quote: ... prior to another round of PCR amplification for 16-18 cycles using either LA-Taq or PrimeSTAR GXL (Takara) at an annealing temperature of 60℃.
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bioRxiv - Neuroscience 2023Quote: ... differentiated HUES9 hESCs (Passage 12-18; RRID:CVCL_0057) were plated on 12-well iMatrix-511 (Cat.No. T303; Takara Bio Inc) coated plates at a density of approximately 3 x 106 cells/well in Synaptojuice medium (adapted from 124 ...
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bioRxiv - Microbiology 2021Quote: ... HEK293T cells grown in 10 ml of medium (3.0 x 105 cells/ml) were transfected with 10 µg of pCAGGS-NP (1-450) using TransIT-293 Reagent (Takara, Shiga, Japan). Three days post-transfection ...
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bioRxiv - Plant Biology 2023Quote: ... and ARR1 (2070 bp) was generated using cDNA of Col-0 as template and ligated into T-vector pMD20 (Takara Bio, Japan). All the primers used for cloning were listed in Table S1 ...
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bioRxiv - Molecular Biology 2024Quote: ... filtered through a 0.45µM PES filter and concentrated with Lenti-X Concentrator (Takara, 631232) according to the manufacturer’s manual.
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bioRxiv - Microbiology 2021Quote: ... The parasite load in the livers and HepG2 cells was evaluated by Taqman-PCR with primers and probes for 18S rRNA and GAPDH following the manufacturer’s instructions of Premix Ex Taq™ (Probe qPCR) (TAKARA). For the SYBR quantitative PCR assay ...
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bioRxiv - Genomics 2021Quote: ... Libraries from 4-cell embryos were also prepared using 18-30 nt gel purified RNA using the SMARTer smRNA-Seq Kit (Clontech) and NEXTflex-Small-RNA-Seq (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... ISG20 expression was routinely induced upon incubation of cells for 18 hours with 1.5 μg/mL of doxycycline (Takara Bio, cat. 631311).
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bioRxiv - Plant Biology 2023Quote: ... and RNA-seq analysis (150 bp PE reads, Novaseq 6000 platform, by Takara Bio; Table S2) as described above.
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bioRxiv - Genetics 2024Quote: Two μg of total RNA was reverse transcribed into cDNA templates using RNA to cDNA EcoDry™ Premix kit including both random hexamer and oligo(dT)18 primers (Takara Bio, 639548). KAPA SYBR® FAST qPCR Master Mix (2X ...
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bioRxiv - Molecular Biology 2023Quote: ... The recombinant AAV2 genome rAAVeCFPrep was constructed by replacing the mCherry coding sequence in the plasmid pAAVtCR (18) with the ECFP coding sequence from pECFP (Clontech, Mountain View, CA, USA). Purified recombinant AAV2 vectors ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The rRNA genes (18S, ITS, 28S) were amplified by polymerase chain reaction (PCR) using the TaKaRa Ex Taq polymerase kit (TaKaRa Bio-medicals, Seoul, Korea). PCR cycling parameters and primer information were followed as in Shazib et al ...
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bioRxiv - Microbiology 2023Quote: ... The amplicon was inserted into SalI- and FseI-digested pE-Akk via In-Fusion Snap Assembly (Takara Bio). The correct sequence of pE_Akk_10880 was confirmed Nanopore sequencing (Plasmidsaurus).
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bioRxiv - Biophysics 2023Quote: ... VLP-containing supernatant was sterile filtered through a 0.45 µm PES filter and fiftyfold enriched using LentiX concentrator following the manufacturer’s protocol (Takara). The VLPs were diluted 1:1 with PBS and stained at a final concentration of 300 nM Flipper or 100 nM NR12S or NR12A before imaging in μ-Slides (18 well glass bottom ...
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bioRxiv - Biochemistry 2022Quote: ... one microgram of total RNA was employed to obtain the corresponding cDNA target sequences using an oligo(dT)18 primer and the PrimeScript RT reagent kit (Perfect Real Time, Takara Bio Inc., Otsu, Shiga, Japan), following the manufacturer’s directions ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was filtered through a 0.45μm PES filter and the viral particles concentrated 100 times using Lenti-X™ Concentrator (Takara) before storage at −80 ...
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bioRxiv - Cancer Biology 2024Quote: KTC1 and PE121410 cells with RBM10 loss-of- function mutations were used to generate dox-inducible RBM10 expressing cells (KTC-RBM10 and PE-RBM10) by transducing the pLVX-Tet-On Advanced vector system (Clontech) with RBM10 cDNA (NM_005676 ...
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bioRxiv - Microbiology 2024Quote: ... the DNA was submitted to sequencing (∼20 million PE reads) via library preparation using the ThruPLEX library preparation kit (Takara, Shiga, Japan), and 150 bp PE sequencing on the Illumina NovaSeq platform (see chapter “Sequencing strategies”).