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Citations for Evrogen :
1 - 35 of 35 citations for SARS CoV 2 Spike Glycoprotein S1 RBD His Tag CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: The RBD nucleotide sequence of SARS-CoV-2 Wuhan-Hu-1 isolate (Genbank accession number MN908947, from 319 to 545 aa) was synthesized (Evrogen, Russia) and cloned into the pCEP4 mammalian expression vector (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... The DNA fragment encoding the RBDv1 ORF with Kozak consensus sequence and C-terminal c-myc and 6xHis tags were obtained by PCR using primers AD-COV-AbsF and AD-RBD-myc6HNheR (listed in Table 1) and Tersus polymerase mix (Evrogen, Moscow, Russia). Synthetic oligo’s ...
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bioRxiv - Biophysics 2021Quote: ... with the mutation C85A and C-terminal 6×His tag) was obtained as a synthetic gene from Evrogen (Russia) and introduced into the pET11 expression vector (Novagen ...
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bioRxiv - Neuroscience 2020Quote: ... Tag-blue fluorescent protein (Tag-BFP, Evrogen), Nlgn3Δ ...
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bioRxiv - Microbiology 2020Quote: ... Primers (S1 Table) were purchased from Evrogen. Plasmids and genetic modifications of the E ...
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bioRxiv - Cell Biology 2020Quote: ... Rabbit anti-Tag(CGY)FP (Evrogen) was used at 1:1000 to detect mTagGFP2 ...
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bioRxiv - Cancer Biology 2021Quote: ... The RFP tag is a monomeric TagRFP from Evrogen.
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A quantitative tri-fluorescent yeast two-hybrid system: from flow cytometry to in-cellula affinitiesbioRxiv - Biochemistry 2019Quote: 3) The coding sequence of the Tag-BFP (from pTag_BFP-Actin, Evrogen) borded with 2 XhoI sites ...
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bioRxiv - Cell Biology 2023Quote: ... and anti-Tag(CGY)FP (1:2000 in 0.5% milk TBST, 4°C o/n, Evrogen AB121). Blots were then incubated with IRDye 800/680 conjugated antibodies (1:10000 in 5% milk TBST ...
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A quantitative tri-fluorescent yeast two-hybrid system: from flow cytometry to in-cellula affinitiesbioRxiv - Biochemistry 2019Quote: ... The coding sequence for Tag-RFP was subsequently introduced in the EcoRI site through PCR from pTag_RFP-Actin (Evrogen), using the primers primSB_0003 and 0004 ...
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bioRxiv - Neuroscience 2023Quote: ... was used to visualize LC neurons and rabbit anti-red fluorescent protein to visualise the fRed tag (tRFP, 1:1000, Evrogen). For visualisation of GRABNE2m expression in the hippocampus ...
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bioRxiv - Cell Biology 2019Quote: ... Membranes were incubated overnight with rabbit anti-tagRFP (which recognise also tagBFP) or rabbit anti-tag(CGY)FP primary antibodies (both from Evrogen, Milan, Italy) at 1:5000 in PBST with 0.5% non-fat dry milk ...
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bioRxiv - Cell Biology 2021Quote: ... Blots were incubated overnight at 4°C with primary antibodies targeting Tag(CGY)FP (1:2000 dilution in 1% milk, Evrogen, Cat#: AB121), HaloTag (1:1000 dilution in 0.5% milk ...
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bioRxiv - Microbiology 2021Quote: ... 2 μl 10x Taq Turbo buffer (Evrogen), 0.25 mM dNTPs ...
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bioRxiv - Biophysics 2019Quote: Monomeric photoswitchable cyan fluorescence protein 2 (PS-CFP2, Evrogen) was C-terminally equipped with an AVI-tag (GLNDIFEAQKIEWHE ...
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bioRxiv - Immunology 2023Quote: Monomeric photoswitchable cyan fluorescence protein 2 (PS-CFP2, Evrogen) featuring an unpaired cysteine residue and C-terminally extended with an AVI-tag (GLNDIFEAQKIEWHE ...
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bioRxiv - Genomics 2020Quote: ... was performed using the Trimmer-2 cDNA normalisation kit (Evrogen) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... 2: rabbit anti-tRFP (1:1,000 dilution, polyclonal, Evrogen, AB233). The following secondary antibodies were used ...
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bioRxiv - Cell Biology 2019Quote: ... or DMEMEGFP-2 anti-bleaching live cell visualization medium (Evrogen, #MCK02), both supplemented with 10% FBS and GlutaMAX-I (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... medium was replaced with live-cell visualization medium DMEMgfp-2 (Evrogen) supplemented with 10 % FBS ...
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bioRxiv - Cell Biology 2021Quote: ... the medium was replaced by live-cell visualization medium DMEMgfp-2 (Evrogen) supplemented with 10% FBS and 2 mM L- glutamine ...
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bioRxiv - Immunology 2021Quote: ... medium was replaced by live-cell visualization medium DMEMgfp-2 (Evrogen, cat. #MC102) supplemented with 10% FBS ...
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bioRxiv - Immunology 2023Quote: ... medium was replaced by live-cell visualization medium DMEMgfp-2 (Evrogen, cat. #MC102) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2019Quote: ... Media was exchanged to DMEMGFP-2 anti-bleaching live cell visualization medium (Evrogen # MCK02) supplemented with 10% FBS and GlutaMAX (Gibco #35050061 ...
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bioRxiv - Molecular Biology 2021Quote: ... the resulting product was cleaned from 2% agarose gel by Cleanup Standard Kit (Evrogen), digested by MluI and HindIII and ligated into pMV306/MluI ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were imaged in DMEM gfp-2 anti-bleaching live-cell imaging medium (Evrogen) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Systems Biology 2019Quote: ... Tissue-specific cDNA libraries were created using the Mint-2 cDNA Synthesis Kit (Evrogen, Russia) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was generated from total RNA (2 μg) by using MMLV reverse transcriptase (Evrogen, Russia). Reverse transcription PCR reaction conditions were as follows ...
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bioRxiv - Genomics 2023Quote: ... 100 ng of amplified products was mixed with 2 μL of 10X DSN buffer (Evrogen) and H2O to 20 μL ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 μg of total RNA and a reaction mixture with MMLV reverse transcriptase (Evrogen, Russia) were used ...
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bioRxiv - Molecular Biology 2020Quote: ... The cDNA library was then normalized using the Trimmer-2 cDNA normalization kit (Evrogen, Moscow, Russia).
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bioRxiv - Genomics 2023Quote: ... After 24-48 hours the coverslips were mounted in the GFP-imaging medium (DMEM-GFP-2, Evrogen) with rutin in a temperature-controlled chamber with CO2 and imaged on an inverted OMX Deltavision microscope in time-lapse mode ...
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bioRxiv - Systems Biology 2020Quote: Cells were plated on 25 mm diameter coverslips (0.17 mm thick) in non-fluorescent media (DMEM gfp-2 with rutin; Evrogen). Coverslips were mounted in a temperature-controlled chamber with CO2 and imaged on an inverted OMXv3 Deltavision microscope in time-lapse mode ...
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bioRxiv - Genomics 2020Quote: ... with cDNAs were incubated at 68 °C for 2 minutes before adding pre-warmed DSN buffer mix with 1 Units of DSN enzyme (Evrogen). The reaction then performed at 68 °C for 20 minutes ...
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bioRxiv - Genomics 2023Quote: ... The libraries were sequenced on an Illumina NovaSeq 6000 platform (S Prime flow cell) with 2 × 150 bp paired-end reads (Evrogen Joint Stock Company, Russia).