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Citations for Rockland :
1 - 39 of 39 citations for ssc mir 376a 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... The RT-PCR products were separated on 2% agarose gel (NuSieve; FMC, Rockland, ME, USA) and visualized by Syber Green (Takara) ...
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bioRxiv - Genetics 2023Quote: ... Mycoplasma was tested using the MycoAlert detection kit (Lonza, Rockland, ME). Cell identity was confirmed using short tandem repeats by PCR profiling ...
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bioRxiv - Neuroscience 2019Quote: ... The incubated membranes were washed three times for 10 min in TBST at RT and incubated for 2 hours at room temperatured with secondary antibodies (Rockland, IRDye Goat anti-mouse 800nm ...
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bioRxiv - Microbiology 2022Quote: ... Both cell lines were regularly monitored for mycoplasma contamination using the MycoAlert mycoplasma detection kit (Lonza Rockland). Primary CD4+ T cells were cultured in RPMI 1640 containing L-glutamine supplemented with 100 U/ml penicillin ...
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bioRxiv - Physiology 2022Quote: ... GFP detection antibody (Cat# 600-401-215, Rockland) was added to the diluted 1:1000 in 0.1% BSA in PBS-T ...
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bioRxiv - Physiology 2023Quote: ... GFP detection antibody (Cat# 600-401-215, Rockland) was added to the diluted 1:1000 in 0.1% BSA in PBS-T ...
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bioRxiv - Microbiology 2023Quote: ... goat anti-GFP to enhance YFP detection (Rockland), and donkey anti-goat Alexa Fluor 488-conjugate (Invitrogen) ...
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bioRxiv - Neuroscience 2020Quote: ... All cells were routinely tested for mycoplasma contamination with MycoAlert Mycoplasma Detection Kit (Lonza, Rockland, ME, USA, Cat N° LT07-318). Chemicals and cell culture reagents were purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Microbiology 2020Quote: ... cell lines were authenticated using STR profiling (Eurofins Genomics) and monitored for mycoplasma contamination using the MycoAlert mycoplasma detection kit (Lonza Rockland, USA). Cells were cultured at 37 °C and 5 % CO2 in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Bioengineering 2019Quote: ... with horseradish peroxidase detection and 3,3’,5,5’-tetramethylbenzidine (TMB, Rockland Immunochemicals, Boyertown, PA) as the substrate 7 ...
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bioRxiv - Microbiology 2022Quote: ... For the detection of FLAG-conjugated proteins we used rabbit anti-DYKDDDDK antibody (Rockland Immunochemicals ...
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Mesenchymal-specific Alms1 knockout in mice recapitulates key metabolic features of Alström SyndromebioRxiv - Physiology 2023Quote: ... Detection of brain mTomato (anti-RFP, rabbit, 1:1000, 600-401-379, Rockland Immunochemicals, USA) and mGFP (anti-GFP ...
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bioRxiv - Immunology 2023Quote: 1×106 cells were washed with two times with PBS (Rockland) and then resuspended in 1ml of 250nM cisplatin and PBS (Fluidigm ...
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bioRxiv - Bioengineering 2022Quote: ... The detection antibody was a peroxidase-conjugated goat anti-human IgG Fc antibody (Rockland 709-1317), used at a 1:5000 dilution in 1% milk in PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary antibodies for infrared fluorescence detection were donkey anti-rabbit-IRDye® 800CW (Rockland, 611-731-127, 1:5000). For infrared fluorescent detection ...
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bioRxiv - Cell Biology 2019Quote: Albumin was measured using a competitive enzyme-linked immunosorbent assay with horseradish peroxidase detection and 3,3’,5,5’-tetramethylbenzidine (TMB, Rockland Immunochemicals, Boyertown, PA) as the substrate (10) ...
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bioRxiv - Microbiology 2022Quote: ... The membranes were washed with TTBS and incubated with 1:5,000 goat anti-mouse HRP for 1 h at RT (Rockland 610-1302) in 5% milk-TTBS ...
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bioRxiv - Microbiology 2022Quote: ... plates were washed three times with 100 μL 3,3′,5,5′-Tetramethylbenzidine (TMB; Rockland, Cat# TMBM-100) using the plate washer and incubated at room temperature for 15 min ...
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bioRxiv - Microbiology 2020Quote: ... HRP activity was detected with a chemiluminescence detection system (Fotodyne Imaging system, USA.) by using PicoMax sensitive chemiluminescence HRP substrate (Rockland Immunochemicals, USA.).
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bioRxiv - Immunology 2022Quote: ... slides were again aspirated and probed with a mixture of fluorescent secondary antibodies that would be used for peptide binding detection consisting of Rabbit Anti-Human IgG DyLight™ 800 (Rockland Immunochemicals), Alexa Conjugated Goat Anti-Human IgA 680 (Jackson Immuno Research Labs ...
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bioRxiv - Molecular Biology 2023Quote: ... Secondary antibodies used for chemiluminescence-based detection were 1:1,000 rat monoclonal anti-mouse Ig HRP (Clone eB144, Mouse TrueBlot ULTRA, Rockland #18-8817-30), 1:10,000 goat anti-rabbit IgG HRP-linked antibody (Cell Signaling Technology ...
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bioRxiv - Developmental Biology 2022Quote: ... the PVDF membranes were incubated for 1 h at RT with corresponding secondary antibodies (1:2000, donkey anti-rabbit-horseradish peroxidase, Rockland, Philadelphia, USA) and were subjected to LAS 3000 imaging workstation (Fujifilm ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR samples were analyzed on 2% agarose gels (Lonza Rockland, ME), 1X TAE ...
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bioRxiv - Neuroscience 2020Quote: ... followed by 2h at RT with the chicken IgY anti-Neurofilament medium chain (NF-M; 1:2000; Rockland Immunochemicals; cat# 212-901-D84) and the mouse IgG1 anti-synaptic vesicular protein 2 (SV2 ...
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bioRxiv - Cell Biology 2019Quote: ... Larvae were washed five times in PBSTD and secondary Dylight 488 donkey anti-mouse IgG antibody (Rockland Immunochemicals) was used at 1:250 in block buffer overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR samples were visualized on 1.5% SeaKem LE agarose (Lonza; Rockland, ME) gels containing 0.5x GelRed (Biotium ...
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bioRxiv - Genomics 2021Quote: ... Blots were then washed 3 times for 3 minutes rotating in PBST and probed with an HRP-conjugated anti-Rabbit secondary antibody (Rockland Trueblot ...
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bioRxiv - Cell Biology 2019Quote: ... Larvae were washed 5 times in IB and incubated with secondary Dylight 488 donkey anti-mouse IgG antibody (Rockland Immunochemicals) at 1:250 in IB for 4 hours at RT or 4°C overnight ...
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bioRxiv - Neuroscience 2019Quote: ... The next day the membranes were washed 3 times with TBST and incubated for 1 h with secondary Trueblot antibodies (Rockland). Proteins were detected by enhanced chemiluminescence reagents (Thermo Fisher Scientific).
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bioRxiv - Immunology 2022Quote: ... The membranes were washed 3 times with PBST and probed with the secondary HRP-conjugated goat anti-human IgG secondary (Rockland) antibody for 1 hour at room temperature ...
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bioRxiv - Immunology 2020Quote: ... The samples were then washed three times with CyFACS and fixed overnight at 4°C in 100 μl of freshly prepared 2% PFA in PBS (Rockland). Samples were then washed twice with Intracellular Fixation & Permeabilization Buffer (eBioscience ...
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bioRxiv - Cell Biology 2019Quote: ... Samples were washed 3 times in PBS at room temperature for 5 minutes each and secondary Dylight 488 donkey anti-rabbit (Rockland Immunochemicals) or Alexa Fluor 568 goat anti-rabbit IgG antibodies (Invitrogen ...
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bioRxiv - Genomics 2021Quote: ... the membrane was washed four times with 1xTBS 0.1% Tween-20 for 5min and then incubated with goat-anti mouse IgG IRDye680® (Rockland cat # 610144002) and goat anti-rabbit IgG IRDye800® (Rockland cat # 611132122 ...
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bioRxiv - Microbiology 2024Quote: ... The PCR product presence was confirmed with gel electrophoresis using the FlashGel® System (Lonza, Rockland, ME, USA). PCR products were then purified with the HighPrep® PCR kit (MagBio Genomics ...
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bioRxiv - Physiology 2019Quote: ... The PCR products were mixed with loading dye and loaded on to a 1.0% SeaKem® GTG® agarose (FMC BioProducts, Rockland ME, USA) containing ethidium bromide ...
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bioRxiv - Genetics 2020Quote: ... All cell lines were tested for mycoplasma with MycoAlertTM Plus kit (Lonza, Rockland, ME) and were mycoplasma-free cells ...
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bioRxiv - Cancer Biology 2021Quote: ... All the cell lines were tested for mycoplasma using the MycoAlertTM Plus kit (Lonza, Rockland, ME, USA) and were mycoplasma-free.
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bioRxiv - Molecular Biology 2022Quote: ... and the protein concentration in each sample was determined using a BCA protein assay kit (Pierce, Rockland, IL, USA). These protein samples were separated using 10% SDS–PAGE and transferred to nitrocellulose membranes (Millipore ...
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bioRxiv - Microbiology 2023Quote: ... TNF-alpha and IL-10 in serum were determined using bead-based Multi-Plex kits (MILLIPLEX® Mouse Cytokine/Chemokine Magnetic Bead Panel 96-Well Plate Assay, Merck KGaA, Cat. No. MCYTOMAG-70K-03, Rockland, MA, USA). In order to remove particulates ...