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Citations for Lonza :
1 - 41 of 41 citations for Saporin Goat Polyclonal affinity purified Alexa488 labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... Purified cells resuspended in EGM-2 media (Lonza) supplemented with 25 ng/ml VEGF165 (PEPROTECH) ...
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bioRxiv - Neuroscience 2024Quote: ... Linear fragments were purified by 1% agarose (Lonza) gel electrophoresis and subsequent QIAquick™ gel extraction (Qiagen) ...
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bioRxiv - Immunology 2021Quote: Purified monocytes were cultured in RPMI1640 (Gibco or Lonza) medium containing 5% FBS (Mediatech) ...
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bioRxiv - Molecular Biology 2019Quote: ... Amplicons were then purified using a 2% agarose (Lonza) gel and the QIAquick Gel Extraction Kit (QIAGEN) ...
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bioRxiv - Cell Biology 2021Quote: ... Amplicons were purified using FlashGels (Lonza, Allendale, NJ, USA) and purified PCR products were used as templates for subsequent PCR amplification ...
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bioRxiv - Microbiology 2019Quote: ... 3 µl of the labeled culture was spotted on glass cover slide and covered with 1.5 % (w/v) SeaKem LE agarose (Lonza) pad in water and visualized by epifluorescence microscopy ...
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bioRxiv - Molecular Biology 2022Quote: ... Following transfection of purified schizonts using an AMAXA nucleofector 4D (Lonza) and P3 reagent ...
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bioRxiv - Bioengineering 2022Quote: ... and gel purified from 0.8% SeaPlaque GTG Agarose (Lonza, Rockland, ME) using a phenol:chloroform:isoamyl alcohol extraction method followed by overnight precipitation at −20°C in 100% ethanol ...
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bioRxiv - Immunology 2019Quote: ... Bone marrow cells were purified using Lymphocyte Separation Medium (Lonza Bioscience) diluted to 90% in DPBS ...
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bioRxiv - Cell Biology 2019Quote: To induce expression of fluorescently labeled proteins DCs were transfected according to manufacturer guidelines using nucleofector kit for primary T cells (Amaxa, Lonza Group). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... then 106 purified schizonts were resuspended in Nucleofector solution (Lonza, NJ, USA) containing 10 μg of linearized pY003V1S plasmid ...
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bioRxiv - Microbiology 2022Quote: ... purified by phenol-chloroform precipitation then resuspended in P3 (Lonza, V4XP-3024) transfection buffer at a concentration of 1ug/ul.
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bioRxiv - Molecular Biology 2021Quote: ... Purified DNA was separated on a 3% NuSieve GTG agarose gel (Lonza #50081). The gel band corresponding to the size of dinucleosomal DNA (∼250 to 350 bp ...
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bioRxiv - Genomics 2020Quote: ... Purified DNA was separated on a 3% NuSieve GTG agarose gel (Lonza #50081). The gel band corresponding to the size of dinucleosomal DNA (∼250 to 350bp ...
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Comprehensive profiling of antibody responses to the human anellome using programmable phage displaybioRxiv - Immunology 2022Quote: ... Vector arms were gel-purified in 1% SeaPlaque agarose (Lonza, Cat No. 50105) in 1X TAE buffer.
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bioRxiv - Molecular Biology 2023Quote: ... Dinucleosomes were purified and excised from a 3% NuSieve GTG agarose gel (Lonza #50081) using Zymoclean Gel DNA Recovery Kit (Zymo #D4008) ...
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bioRxiv - Molecular Biology 2023Quote: ... All PCR products and double-stranded DNAs were purified with agarose gels (Lonza, 50004) and the GeneJet Gel Extraction Kit (Thermo Fisher ...
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bioRxiv - Bioengineering 2019Quote: ... and gel purified using Seakem GTG agarose (for nucleic acid recovery) (Lonza, cat no. 50070), agarose dissolving buffer (ABD ...
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bioRxiv - Immunology 2022Quote: ... Purified B cells were then cultured at 0.5 ×106 cells/mL in RPMI 1640 (Lonza) containing penicillin (100 IU/ml) ...
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bioRxiv - Microbiology 2022Quote: ... Mature schizonts were purified by a Nycodenz gradient and transfected using Amaxa electroporation system (Lonza). Transfected merozoites were injected into the tail vein of a BALB/c mouse ...
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bioRxiv - Molecular Biology 2023Quote: ... Dinucleosome-sized DNA fragments were purified and excised from a 3% NuSieve GTG agarose gel (Lonza) using the Zymoclean Gel DNA Recovery Kit (Zymo Research) ...
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bioRxiv - Cancer Biology 2024Quote: ... 3ug of purified EVs were electroporated in 20μL of SF buffer (#V4XC-2032, Lonza, Basel, Switzerland) with 0.4μg of CRE-expressing plasmid (Addgene #172433 ...
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bioRxiv - Bioengineering 2019Quote: ... The cells were transfected with purified linear pFSC005 using the Nucleofector® 2b electroporator (Lonza, Morristown, NJ), following the Lonza Amaxa® Cell Line Nucleofector® Kit V optimized transfection protocol for Jurkat Clone E6-1 cells ...
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bioRxiv - Neuroscience 2022Quote: ... RNA components (IDT) and purified Cas9 (UCB Macrolab) were nucleofected in the SH- SY5Y cell line (Lonza 4D-Nucleofector core unit ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA was prepared using ethanol precipitation and electroporation of purified schizonts was carried out using Nucleofactor technology (Lonza). Transfection mixtures were then injected intravenously into a mouse ...
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bioRxiv - Cancer Biology 2023Quote: ... Human T cells were purified with EasySepTM beads and resuspended in Human T Cell Nucleofector™ Solution (Lonza). For each cuvette ...
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bioRxiv - Immunology 2021Quote: ... The siRNA duplexes were used to transfect purified human naïve B cells using the Human B Cell NucleofectorTM Kit (VPA-1001, LONZA). Transfected B cells were then stimulated with CpG ODN 2395 plus IL-2 and IL-21 for 96 h before genomic DNA extraction for analysis of Sμ-σδand Sμ-Sγ1 DNA recombination ...
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bioRxiv - Synthetic Biology 2021Quote: ... The product was purified and 2 µg were added to the RNP and diluted in 100 µL P3 nucleofection buffer (Lonza). This mixture was nucleofected with 2×106 stimulated human primary T cells using the 4D-Nucleofector (Lonza ...
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bioRxiv - Molecular Biology 2020Quote: ... CD34+ cells were purified from the apheresis PBMC collection via immunoabsorption and cultured overnight in at 37°C in X-VIVOTM 10 (Lonza) supplemented with 1% HSA (Baxter ...
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bioRxiv - Genetics 2020Quote: ... All nucleofections were performed using 106 freshly-purified CD4 T cells in 100 μl nucleofection buffer using a Nucleofector 2b device (Lonza), based on previous optimisation studies (data not shown) ...
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bioRxiv - Microbiology 2020Quote: ... 2019): The plasmid was linearized using PsiI-V2 and purified using phenol-chloroform precipitation and resuspended in P3 solution (Lonza). Successful linearization was confirmed using agarose-gel electrophoresis ...
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bioRxiv - Neuroscience 2020Quote: Primary human cortical astrocytes purified from 17-18 weeks gestation human fetuses of indeterminate sexes were used in this experiment (Lonza). Astrocytes were expanded using the astrocyte growth medium bulletkit according to the manufacturer’s instructions at 37°C ...
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bioRxiv - Microbiology 2021Quote: Purified SARS-CoV-2 RBD and SED (Spike ectodomain) were diluted in DPBS (without Ca and Mg, Lonza, 17-512F) and coated onto 96-well plates (MaxiSorp - ThermoFisher or High binding - Greiner Bio-one ...
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bioRxiv - Genomics 2023Quote: ... purified Percoll-enriched mature schizonts (∼20 μl packed volume) were suspended in 100 μl of P3 primary cell solution (Lonza) containing 60 μg of linearized repair plasmid DNA (repair plasmid 1 and 2 separately in two separate transfection experiments ...
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bioRxiv - Microbiology 2022Quote: ... Residual endotoxins in the purified phage preparations were determined by the Limulus Amebocyte Lysate (LAL) Kinetic-QCL Kit (Lonza, Basel, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Percoll-purified (82) late-schizont-stage parasites were transfected with 50 µg of plasmid DNA using Amaxa Nucleofector 2b (Lonza, Switzerland) as previously described(83) ...
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bioRxiv - Immunology 2021Quote: ... The purified CD19+ B cells were cultured in the chemical-defined and serum-free X’VIVO 15 medium (Lonza, Walkersville, MD, USA), in the absence or presence of 100 U/ml penicillin ...
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bioRxiv - Molecular Biology 2022Quote: ... Percoll-purified (70) parasites at late schizont stage were transfected with 50 μg plasmid DNA using Amaxa Nucleofector 2b (Lonza, Switzerland) as previously described (78) ...
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bioRxiv - Cell Biology 2023Quote: ... Percoll-purified [131] parasites at late schizont stage were transfected with 50 µg plasmid DNA using Amaxa Nucleofector 2b (Lonza, Switzerland) as previously described [135] ...
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bioRxiv - Synthetic Biology 2022Quote: ... were ligated to the target fragments and full-length ligation products were purified via gel electrophoresis (2% MetaPhor agarose, Lonza, Basel, Switzerland). Purity and concentration of extracted fragments were determined using capillary electrophoresis (Fragment Analyser ...
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bioRxiv - Immunology 2023Quote: ... The levels of endotoxin in the purified protein samples were assessed using the QCL-1000 Limulus amebocyte lysate system (Lonza, Basel, Switzerland), and it was found that the recombinant proteins had endotoxin levels of ∼ 4 pg/µg ...