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Citations for Lonza :
1 - 40 of 40 citations for Etchant 001 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... program X-001 on the Nucleofector 2b (Lonza). Puromycin selection was performed with 2ug/ml for Calu6 and H2228 or 4ug/ml for H520 ...
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bioRxiv - Cell Biology 2022Quote: ... Nucleofection (Nucleofector kit V, Lonza, program V-001) following the manufacturer’s instructions was used for overexpression experiments in THP-1 cells ...
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bioRxiv - Immunology 2023Quote: ... using a Nucleofector 2b (Lonza, program Y-001). After nucleofection ...
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bioRxiv - Molecular Biology 2020Quote: ... with program X-001 of the Amaxa Nucleofector (Lonza). Following each transfection ...
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bioRxiv - Cell Biology 2022Quote: ... following the X-001 protocol provided by Amaxa Nucleofector (Lonza). After 24 h ...
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bioRxiv - Immunology 2023Quote: ... plasmids using the X-001 program on a Nucleofector (Lonza) and rested overnight in T cell medium (Lonza) ...
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bioRxiv - Cancer Biology 2024Quote: ... with programme X-001 using a Nucleofector-2b device (Lonza). Construct expression and ectopic protein localisation was confirmed using fluorescence microscopy.
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bioRxiv - Immunology 2024Quote: ... Cells underwent nucleofection with RNP complexes using program X-001 (Lonza). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... U2OS cells were nucleo-transfected (Amaxa nucleofector program U2OS X-001, Lonza) with Amaxa kit V ...
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bioRxiv - Microbiology 2020Quote: ... and the X-001 program or using the Amaxa 4D-Nucleofector (Lonza) and the FI-115 programme (for the Cytomix and P3 solutions respectively ...
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bioRxiv - Immunology 2023Quote: ... Cells were electroporated using the X-001 program on a Nucleofector (Lonza) using a mouse T cell nucleofection kit (Lonza ...
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bioRxiv - Microbiology 2021Quote: ... Transfection was carried out using program X-001 of the Amaxa Nucleofector IIb (Lonza) electroporator in 2 mm gap cuvettes ...
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bioRxiv - Microbiology 2022Quote: ... Transfection was carried out using program X-001 of the Amaxa Nucleofector IIb (Lonza) electroporator in 2 mm gap cuvettes ...
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bioRxiv - Cell Biology 2024Quote: ... Transfections were performed with Amaxa Nucleofactor IIb (Lonza Cologne AG, Germany, program X-001) using BTX electroporation cuvettes (45-0125) ...
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bioRxiv - Cell Biology 2021Quote: ... Constructs were transiently transfected by nucleofection in U2OS cells (Amaxa nucleofector program U2OS X-001, Lonza) as described previously40.
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bioRxiv - Biochemistry 2020Quote: ... 50 mM HEPES pH 7.3) using the X-001 program of an Amaxa Nucleofector II (Lonza, Switzerland) (Burkard et al. ...
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bioRxiv - Microbiology 2020Quote: ... Nucleofection was performed using the program X-001 for Jurkat T-cells (Amaxa™ Nucleofector™ II, Lonza). Cells were cultured in RPMI-1640 medium media supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2023Quote: ... and nucleofected with 5 μg of a plasmid using program X-001 in the Nucleofector IIb device (Lonza) according to the manufacturer’s procedure ...
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bioRxiv - Immunology 2023Quote: ... or KSQ-001 cells were thawed and rested in serum-free X-VIVO15 media (Lonza, Cat# 04-418Q) overnight ...
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bioRxiv - Immunology 2021Quote: ... 2×106 cells were nucleofected with 2μg of pcDNA3.1+ plasmid per reaction (Lonza-AMAXA program X-001, Nucleofector 2b). 24 hours after transfection ...
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bioRxiv - Biophysics 2021Quote: ... The cell suspension was transferred to an electroporation cuvette and electroporated using program X-001 on an Amaxa Nucleofector II (Lonza). Cells in the cuvette were mixed with 300 μl of DMEM ...
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bioRxiv - Genetics 2022Quote: ... Libraries were electroporated into K562 cells in 100ul volumes using the Amaxa system (Program X-001, Neucleofactor kit V, Lonza). We performed 6 independent replicates with each replicate consisting of ∼2×108 cells ...
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bioRxiv - Microbiology 2022Quote: ... 3 to 4×107 cells were transfected with 3 to 8 μg of DNA in 100 μl bloodstream form transfection buffer (Schumann Burkard, 2011) using program Z-001 on the Amaxa Nucleofector (Lonza). Limiting dilutions of the parasites were prepared in 48-well plates ...
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bioRxiv - Microbiology 2022Quote: ... transfection was performed with 3.5 μg of PF16eYFPNeo introduced into 108 cells using a Human T-cell kit and IIb Nucleofector set to program X-001 (Lonza). Other transfections described in this work used the same conditions except with Tb-BSF buffer (90 mM sodium phosphate ...
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bioRxiv - Molecular Biology 2022Quote: ... The suspension was transferred to an electroporation cuvette and electroporated using the Q-001 program on an Amaxa Nucleofector II (Lonza). The cells were recovered from the cuvette ...
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bioRxiv - Immunology 2023Quote: ... The transfection was carried out by nucleofection using Amaxa® Cell Line Nucleofector Kit V (Program X-001, Lonza, USA). At 24 h after transfection ...
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bioRxiv - Cell Biology 2023Quote: ... 2005) using Amaxa Basic Parasite Nucleofector Solution 1 using the X-001 program of an Amaxa Nucleofector II (Lonza, Switzerland). Clones were selected by growth in medium containing phleomycin (2.5 μg/ml ...
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bioRxiv - Molecular Biology 2022Quote: ... 50 µl sterilised DNA was mixed with 5E6 cells submerged in 200 µl of 1.25x Tb-BSF buffer (final transfection volume 250 µl with 1x Tb-BSF buffer) and transfected using one pulse with program X-001 on an Amaxa Nucleofector IIb (Lonza). Cells were recovered in M199 culture medium as specified above and 8-16 hours post transfection the required selection drug was added ...
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bioRxiv - Molecular Biology 2023Quote: ... All this volume was transferred to an electrolytic cuvette and transfection was performed using the X-001 program of Amaxa Nucleofector instrument (LONZA). The culture was maintained in M199 at 26°C before selection with the appropriate drugs (16μg/mL hygromycin-B ...
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bioRxiv - Molecular Biology 2023Quote: ... Parasites were then subjected to one pulse using X-001 program in the Amaxa Nucleofector 2b (Lonza Cologne AG, Germany). Transfected cells were cloned after 6 h in 24-well dishes with the appropriate selective drugs (2.5 μg/ml of G-418 ...
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bioRxiv - Immunology 2024Quote: ... 5x1e6 cells were transfected with 5μg/plasmid DNA/million cells using a LONZA electroporator (program X-001) and LONZA electroporation kit for primary mouse T cells (VPA-1006; LONZA) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells and DNA were resuspended in 250 μl of phosphate buffer and electroporated using the program X-001 (Amaxa Nucleofector IIb; Lonza). Parasites were recovered in M199 overnight in M199 with 10 % additional FBS ...
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bioRxiv - Immunology 2024Quote: ... siRNAs were added to 2 µM and electroporation was performed using the program U-001 on the 3D-Nucleofector (Lonza). The siRNAs targeting Clever-1 ...
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bioRxiv - Molecular Biology 2022Quote: ... and then mixed with 5 μg purified sgRNA expression vector or the empty vector as a negative control before nucleofection using program Q-001 of a Nucleofector™ 2b (Lonza). To enrich transfectants ...
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bioRxiv - Cell Biology 2024Quote: ... brucei 13-90 cells were electroporated with 10 µg of linearised plasmid DNA using the Amaxa Basic Parasite Nucleofector Kit 1 and Nucleofector II device (program X-001) (Lonza, Switzerland).
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bioRxiv - Cell Biology 2023Quote: ... TC28a2 reporter lines were generated using an identical manner to HEK293T except DNA was introduced using electroporation using the X-001 program on the Nucleofector 2b (Lonza, AAB-1001) in combination with Nucleofector Kit V (Lonza ...
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bioRxiv - Microbiology 2021Quote: ... electroporated with 10 μg of linearised plasmid DNA or PCR product using the Amaxa Basic Parasite Nucleofector Kit 1 and Nucleofector II device (Lonza, Switzerland, program X-001).
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bioRxiv - Cancer Biology 2021Quote: ... cells were also transfected with electroporation using Amaxa™ Nucleofector Kit R (Cat.no.VCA-100) and the Nucleofector™II device (program W-001, Lonza Group AG, Cologne, Germany) as suggested by the manufacturer.
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bioRxiv - Immunology 2024Quote: ... For transient transfection 1e6 Jurkat cells were transfected with 2 μg DNA using the LONZA electroporator (program X-001) and Nucleofector Kit V (Lonza, catalog no. VCA-1003) 24 h prior to imaging ...
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bioRxiv - Synthetic Biology 2024Quote: ... The cell/DNA suspension was transferred into the certified Nucleofector® cuvette and the V-001 program of Nucleofector® 2b Device (Lonza, AAB-1001) was applied ...