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Citations for Lonza :
451 - 500 of 611 citations for tert Butyl 10 oxo 4 9 diazaspiro 4.5 decane 4 carboxylate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... and 10 μg plasmid DNA in a volume of 25-30 μL of AD1 Solution (Lonza Bioscience) was put as a drop on the slice ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10% FBS (Hyclone) and Microvascular Endothelial Growth Medium growth factor kits (Lonza Cat# CC-4147). HLMVECs between passages 5 and 9 were used for experiments ...
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bioRxiv - Bioengineering 2024Quote: ... Suspensions were centrifuged for 10 minutes at 300g and digested with ACK lysing buffer (Lonza, Walkerville, VA). The cells were then washed with PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Both bone marrow and mammary gland were treated with ACK Lysing Buffer (10-548E; Lonza, Basel, Switzerland) to lyse red blood cells ...
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bioRxiv - Microbiology 2023Quote: ... BHK-21 Clone 13 (ATCC number CCL-10) were cultured in Glasgow Minimum essential medium (GMEM, Lonza) supplemented with 10% (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... pH 7.3) containing 10 μg DNA and transfected by electroporation using an AMAXA Nucleofector® Device (Lonza) with program “X-001 free choice” ...
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bioRxiv - Developmental Biology 2024Quote: ... For BMP stimulation cells were transferred into serum-free medium X-VIVO 10 (Lonza, Walkersville, Maryland, USA). After 24 h in the serum-free medium treatment with BMP ligands was performed for 48 h as described above for HSPCs.
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bioRxiv - Genomics 2024Quote: ... and for pre-clinical and clinical-scale up experiments we used X-VIVO 10 (Lonza, 04-743Q) supplemented with 100 ng/mL of SCF ...
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bioRxiv - Genomics 2024Quote: ... All media was supplemented with 10% Fetal Bovine Serum (FB12999102, FisherScientific) and 1% Penicillin-Streptomycin (Lonza,17602E). Cells were cultured at 37℃ with 5% CO2 in 10 cm tissue culture dishes until confluent.
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bioRxiv - Cancer Biology 2020Quote: ... and 10 ng/mL basic fibroblast growth factor or MEGM™ culture medium (Lonza, cat. no. CC-3150), completed as per manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... and stained with 10 × SYBR Green I (SYBR® Green I Nucleic Acid Stain; Lonza, Rockland, ME, USA) in 1×TAE buffer for 3 min ...
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bioRxiv - Bioengineering 2022Quote: ... VERO cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 2 mM glutamine (Lonza). Cells were dislodged ...
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bioRxiv - Genomics 2020Quote: ... MEFs were cultured in MEF media composed of Dulbecco’s Modified Eagle medium (DMEM) supplemented with 10% FBS (Lonza), 100ug/mL Primocin (Invivogen ...
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bioRxiv - Physiology 2022Quote: ... Media is removed and the cell pellet is resuspended in 3mL of ACK lysis buffer (Lonza 10-548E) for 2 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... Electrophoresis of 10 µl of RT-PCR products was performed using 3% agarose (SeaKem LE Agarose by Lonza) with molecular ladder gel containing 0.5 µg/ml ethidium bromide in x0.5 tris-acetate-ethylenediaminetetraacetic acid (TAE ...
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bioRxiv - Molecular Biology 2023Quote: ... cell lines were maintained in high glucose Dulbecco’s modified Eagle’s medium (DMEM) by adding 10% fetal bovine serum (FBS, Lonza), 2 mM L-glutamine ...
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bioRxiv - Pathology 2023Quote: ... and the cell pellet was resuspended in 10 mL of Endothelial Cell Growth Medium-2 (EGM-2, Lonza). The cell suspension was plated into a 75 cm2 tissue culture treated flask and cultured in a 5% CO2 incubator at 37°C ...
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bioRxiv - Immunology 2023Quote: ... The neutrophil pellet was then cleared of remaining red blood cells using ACK lysis buffer (Lonza #10-548E). For subsequent experiments ...
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bioRxiv - Genomics 2023Quote: ... MEFs were cultured in MEF media composed of Dulbecco’s Modified Eagle medium (DMEM) supplemented with 10% FBS (Lonza), 100 μg/mL Primocin (Invivogen) ...
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bioRxiv - Immunology 2023Quote: ... 1999) cultured in I10 media (Iscove’s modified Dulbecco’s Medium, 10% FBS, 1:1000 MycoZap; Thermo Fisher and Lonza) supplemented with 100 ng/mL IL-21 (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: ... program A-30 with 10 µg of plasmid DNA and 1 µg of eGFP control plasmid (Amaxa, Lonza) was used ...
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bioRxiv - Immunology 2024Quote: ... 10-fold serial dilutions of viral supernatant in serum-free Dulbecco’s Modified Eagle Medium (DMEM; Lonza, #12-614Q) were overlaid on Vero E6-TMPRSS2-hACE2 confluent monolayers and adsorbed for one hour at 37°C and 5% carbon dioxide (CO2) ...
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bioRxiv - Immunology 2024Quote: ... 1-10 million of pre-activated P14 cells were resuspended in 20 μl of P4 Nucleofection Buffer (Lonza). This cell suspension was then mixed with sgRNA/Cas9 and then incubated at room temperature for 2 mins ...
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bioRxiv - Biophysics 2021Quote: ... 10 pmol of the cholesterol-modified reverse primer and 1μL 20X SYBR® Green I Nucleic Acid Stain (Lonza) were mixed with the KAPA2G Fast HotStart ReadyMix in a total volume of 20 μL ...
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bioRxiv - Developmental Biology 2021Quote: HEK293T/17 cells (ATCC CRL-11268) were grown in Dulbecco’s modified Eagle medium (Hyclone) supplemented with 10% fetal bovine serum (Lonza) and penicillin-streptomycin (Gibco) ...
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bioRxiv - Bioengineering 2022Quote: NK-92 cell line was acquired from the American Type Culture Collection and maintained in X-VIVO 10 (Lonza) supplemented with 20% FBS and 0.02 mM folic acid (Millipore Sigma ...
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bioRxiv - Immunology 2021Quote: ... density gradient centrifugation (20 min, 800g) and culture for 12-18 h in X-VIVO 10 TM medium (Lonza) supplemented with gentamicin ...
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Dynamic chromatin organization and regulatory interactions in human endothelial cell differentiationbioRxiv - Developmental Biology 2022Quote: ... and re-seeded onto 0.2% gelatin-coated 10-cm dishes with 5.0 × 105 cells per dish in Endothelial Cell Growth Medium (EGM; Lonza) containing 20 ng/mL VEGF ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were maintained in culture for the indicated time in the following medium: X-VIVO 10 (Lonza, BE04-380Q) supplemented with 20% BIT 9500 Serum Substitute (StemCell technologies ...
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bioRxiv - Biochemistry 2020Quote: HEK293T cells were maintained in DMEM (Dulbecco’s modified Eagle’s high glucose medium) supplemented with 10% (v/v) FBS (foetal bovine serum) and 1% antibiotic/antimycotic (Lonza) in a humidified incubator with 10% CO2.
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bioRxiv - Biochemistry 2021Quote: ... DNA (10 μg) was transfected into SmOx P9 cells using a 4D nucleofector system (Lonza Group AG, Basel, Switzerland) with program FI-115 ...
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bioRxiv - Cell Biology 2021Quote: ... were grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% (v/v) fetal bovine serum (FBS) and 1X penicillin/streptomycin/amphotericin B (Lonza) at 37°C with 5% CO2 ...
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bioRxiv - Cell Biology 2022Quote: ... together with a single-stranded donor (ssODN) were delivered into mito-GFP1-10 cells using the Nucleofector system (Lonza). Cell line constitutively expressing mito-mCherry-GFP1-10 was generated by transducing ARPE-19 cells with lentiviral mito-mCherry-GFP1-10 and enriched for mCherry positive cells via cell sorting ...
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bioRxiv - Immunology 2022Quote: ... PBMC were seeded at 1.5×106 cells per cm2 in X-VIVO 10 media (Cat. No. BE04-743Q, Lonza) +1% penicillin-streptomycin (Cat ...
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bioRxiv - Genetics 2022Quote: ... were grown in 10cm/10ml dish with the Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and MycoZapTM Plus-CL (Lonza). Cells were transfected with pJC_GAA100 plasmid by using JetPRIME® (Polyplus-transfection ...
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bioRxiv - Cancer Biology 2024Quote: ... Red blood cell lysis was performed for 5 min at room temperature in ACK lysing buffer (Lonza, #10-548E). For flow cytometry ...
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bioRxiv - Bioengineering 2024Quote: hMSC were plated at 15,000 cells/well in 24-well plates and grown overnight in a growth medium containing 10% FBS (Lonza). When cells reach 80% confluency ...
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bioRxiv - Bioengineering 2024Quote: hMSC were plated at 15,000 cells/well in 24-well plates and grown overnight in a growth medium containing 10% FBS (Lonza). Once cells reached 80% confluency ...
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bioRxiv - Microbiology 2023Quote: ... Size fractionation for RACE studies was performed by running 10 µg of hot phenol-isolated RNA on a 1.25% MOPS agarose gel (Lonza) in 1x MOPS buffer with the Riboruler low range RNA ladder (Thermo ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% tetracycline-free and heat inactivated fetal bovine serum (FBS) and 100 U/mL penicillin/streptomycin (Lonza). Cell lines were grown at 37 °C in a humidified atmosphere of 5% CO2 and were tested for absence of mycoplasma contamination.
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bioRxiv - Biochemistry 2023Quote: ... DNA (10 μg) was transfected into SmOx P9 cells using a 4D nucleofector system (Lonza Group AG, Basel, Switzerland) with program FI-115 ...
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bioRxiv - Developmental Biology 2023Quote: ... containing 10 μg of the plasmid DNA and transfected using the program DS-112 of the 4D-nucleofector (Lonza). NSCs were harvested 48 hours post-nucleofection ...
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bioRxiv - Immunology 2023Quote: moDCs were cultured with allogenic naïve CD4+ T cells in a 1:10 ratio in complete IMDM (IMDM (BE12- 722F, Lonza) with 100 U/mL penicillin ...
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bioRxiv - Immunology 2023Quote: ... one plate was prepared with 100 µl IMDM/Glutamax/ 10% FBS containing 2× MycoZap Plus-PR (Lonza #VZA-2021), 100 ng/ml human IL-2 (GoldBio #1110-02-50) ...
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bioRxiv - Cell Biology 2023Quote: ... Viral particles were formulated into either X-VIVO™ 10 or X-VIVO™ 15media (Lonza, Walkersville, MD, USA) or phosphate buffered saline containing 1% human serum albumin (Grifols Biologicals ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 10% (v/v) fetal bovine serum (FBS; Biowest, S181B) and 0.2% (v/v) MycoZap (Lonza, VZA-2012) in a humidified atmosphere of 5% CO2 at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... Size fractionation for RACE studies was performed by running 10 µg of hot phenol-isolated RNA on a precast 1.25% MOPS agarose gel (Lonza) in 1x MOPS buffer (Quality Biological ...
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bioRxiv - Microbiology 2024Quote: ... The final CoMe mix was filtered sterile using a 0.22 μm filter and supplemented with 10 ng/ml EGF (CC-4107, Lonza) and 1 ug/ml hydrocortisone (H0888 ...
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bioRxiv - Bioengineering 2024Quote: ... The cells (passages between 10-12) were cultured in growth medium (FBM™ Fibroblast Growth Basal Medium (Lonza, Germany) supplemented with 1% penicillin−streptomycin (P/S ...
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bioRxiv - Biophysics 2024Quote: ... Human mesenchymal stem cells (hMSCs, ATCC) were cultured in DMEM with 10% FBS and differentiated with osteogenic medium (Lonza) on day 2 post-seeding ...