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Citations for Constant Systems :
1 - 15 of 15 citations for Phosphatase Inhibitor Cocktail 2 Tubes 100X since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... pH 7.5) supplemented with Protease Inhibitor Cocktail and then broken using a cell disruptor (Constant Systems Limited). The lysates were centrifuged at 12,000 g for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... 20 mM imidazole) supplemented with protease inhibitor cocktail (cat. PIC002.1, BioShop) and lysed using a benchtop cell disruption unit (Constant Systems Ltd.). Affinity chromatography (HIS-Select™ Nickel Affinity Gel ...
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bioRxiv - Plant Biology 2022Quote: ... Cells were resuspended in phosphate buffer pH 7.0 (2 mL for 1 mL pellet) containing 300 μl of protease inhibitor 1x (Constant systems Ltd. United Kingdom).
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bioRxiv - Cell Biology 2020Quote: ... 1 mM TCEP and protease inhibitors) and lysed using ultrasonic homogenizer (Constant Systems Ltd). The proteins were first purified by affinity chromatography using TALON metal affinity resin (TaKaRa) ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.5 mM TCEP and protease inhibitors) and lysed using a cell disrupter (Constant Systems Ltd). The lysate was cleared by centrifugation for 45 min at 48.000 xg and loaded onto a HisTrap FF 5-ml column (GE Healthcare) ...
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bioRxiv - Plant Biology 2019Quote: ... 0.2% v/v TWEEN20 and EDTA-free protease inhibitor tablets and lysed using a cell disruptor (Constant Systems Ltd) at 14,000 psi ...
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bioRxiv - Cell Biology 2022Quote: ... cracked in a cell disruptor (Constant Systems; 2 cycles at 25 kpsi), and clarified by centrifugation (45 min ...
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bioRxiv - Molecular Biology 2021Quote: ... supplemented with 50 μg/mL DNase I and EDTA-free protease inhibitors) and lysed by passage through a cell disruptor at 24 kPSI (Constant Systems). Lysate was cleared by centrifugation (39,000 × g ...
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bioRxiv - Microbiology 2020Quote: One protease inhibitor tablet and DNase were added to the completely thawed cell samples before being lysed using a cell disrupter (Constant Systems) at 23 kilopounds per square inch (KPSI) ...
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bioRxiv - Biophysics 2023Quote: ... 2 mM CaCl2 through a French press cell disrupter at 25,000 psi (Constant Systems Ltd.) followed by centrifugation at 20,000 x g where the clear lysate was subsequently loaded onto a 5 mL Ni-NTA Sepharose column (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were harvested 2-3 hours later and lysed using a cell disrupter (Constant Systems Ltd.). Proteins were purified from inclusion bodies using Nickel affinity chromatography on prepacked HisTrap FF columns (Cytiva ...
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bioRxiv - Biochemistry 2024Quote: ... 2 mM β-mercaptoethanol) and disrupted by high pressure using a One Shot Cell Disruptor (Constant Systems) at 1.9 kbar ...
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bioRxiv - Plant Biology 2020Quote: ... 5 mM EDTA before being lysed at 2°C with an automatic cell pressure lysis machine at 0.3 KBar (Constant Systems) in homogenization buffer (50 mM Tris adjusted to pH 8.0 with MES ...
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bioRxiv - Plant Biology 2021Quote: ... in the presence of 5 mM EDTA and lysed by 1-2 passes through a cell disrupter operated at 35-37 kpsi (TS-Series 0.75 kW; Constant Systems). Membranes were collected from the suspension by centrifugation at 200,000 x g for 90 mins (45Ti rotor ...
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bioRxiv - Microbiology 2021Quote: ... The cell suspensions were incubated with DNase I (50 μg ml-1) and MgCl2 (2 mM) for 10 min at room temperature before extraction with a continuous-flow cell disruptor (Constant Systems) at 277 K and a pressure between 17-18 KPSI ...