Labshake search
Citations for Constant Systems :
1 - 14 of 14 citations for ESM 1 Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2022Quote: ... 1 mM DTT) and cells lysed by 1 cycle of French Press at 25 kPsi (Constant Systems Ltd MC Cell Disrupter). The lysate was centrifuged at 4 °C and 20000 g for 30 min and the supernatant applied to a gravity flow column with 4 mL pre-equilibrated Ni-NTA Sepharose resin (GE Healthcare) ...
-
bioRxiv - Cell Biology 2020Quote: ... 1 mM TCEP and protease inhibitors) and lysed using ultrasonic homogenizer (Constant Systems Ltd). The proteins were first purified by affinity chromatography using TALON metal affinity resin (TaKaRa) ...
-
bioRxiv - Microbiology 2023Quote: ... 1% NP-40) and then disrupted by French press system (Constant Systems Ltd, Daventry, UK). The lysed cells were centrifuged at 12,000g ...
-
bioRxiv - Molecular Biology 2021Quote: ... 5% glycerol) supplemented with 1 mM of PMSF and disrupted using Cell Disruptor CF (Constant Systems). The lysate was cleared by centrifugation ...
-
bioRxiv - Biochemistry 2020Quote: ... and 1 mM MgSO4) before lysing them by three passages through a Constant cell disruptor (Constant Systems) at 39 000 psi ...
-
bioRxiv - Neuroscience 2020Quote: ... 1 mM PMSF) and lysed by cell disruption (Constant systems LTD 0.75KW, two passes with 25K Psi) at 4 °C ...
-
bioRxiv - Biochemistry 2022Quote: ... 1 mM β-mercaptoethanol (pH 7.5)) and lysed with a cell disruptor (Constant Systems, Daventry Northants, UK)) ...
-
bioRxiv - Biochemistry 2022Quote: ... 1 mM β-mercaptoethanol (Buffer A) and lysed with a cell disruptor (Constant Systems, Daventry Northants, UK). The supernatant was loaded onto HisTrapTM HP 5 ml column (GE healthcare ...
-
bioRxiv - Plant Biology 2021Quote: ... in the presence of 5 mM EDTA and lysed by 1-2 passes through a cell disrupter operated at 35-37 kpsi (TS-Series 0.75 kW; Constant Systems). Membranes were collected from the suspension by centrifugation at 200,000 x g for 90 mins (45Ti rotor ...
-
bioRxiv - Microbiology 2021Quote: ... The cell suspensions were incubated with DNase I (50 μg ml-1) and MgCl2 (2 mM) for 10 min at room temperature before extraction with a continuous-flow cell disruptor (Constant Systems) at 277 K and a pressure between 17-18 KPSI ...
-
bioRxiv - Microbiology 2022Quote: ... Thawed cell pellets were supplemented with an additional 1 mM PMSF and lysed using a One Shot homogeniser (Constant Systems Ltd) at 20 KPSI at 4°C and then centrifuged at 19 000 RPM in a JA-25.50 rotor (Beckman Coulter ...
-
bioRxiv - Microbiology 2020Quote: ... Bacterial pellets were resuspended in 1/50th of the original culture volume and lyzed using a cell disruptor (One shot model, Constant System Inc.). Proteins were purified by affinity chromatography using a GSTrap HP affinity column (GE Healthcare ...
-
bioRxiv - Plant Biology 2022Quote: ... Cells were resuspended in phosphate buffer pH 7.0 (2 mL for 1 mL pellet) containing 300 μl of protease inhibitor 1x (Constant systems Ltd. United Kingdom).
-
bioRxiv - Molecular Biology 2023Quote: ... cell pellets were lysed at 4°C using high-pressure homogenization at 25 Kpsi (1 Kpsi = 69 _ 103 MPa) (Constant System Ltd, UK) in lysis buffer [50 mM Tris-HCl pH:7.5 ...