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Citations for Constant Systems :
1 - 38 of 38 citations for 7 Oxa 12 thia spiro 5.6 dodecan 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Lysis was performed using the One-shot cell disrupter at 1.5 Kbar (One shot model, Constant Systems Ltd). Lysates were centrifuged for 30 min at 30000 x g in 4 °C and the resulting supernatants were gently mixed with Ni-NTA Agarose beads (Qiagen ...
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bioRxiv - Cell Biology 2022Quote: ... the pellet was resuspended in one volume of RP buffer and passed through a French press (One shot cell disruptor, Constant Systems LTD, Daventry, UK) at 2.2 Kpsi ...
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bioRxiv - Cell Biology 2024Quote: ... the pellet was resuspended in one volume of RP buffer and passed through a French press (One shot cell disruptor, Constant Systems LTD, Daventry, UK) at 2.2 Kpsi ...
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bioRxiv - Microbiology 2021Quote: ... Cells were disrupted twice using a One-Shot disrupter (Constant Systems). The lysate was then centrifuged for 15 minutes at 20,000 rcf ...
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bioRxiv - Microbiology 2022Quote: ... passed through a One-Shot cell disruptor (Constant systems, Northants, UK) at 30 kPsi ...
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bioRxiv - Microbiology 2019Quote: ... and lysed using the One Shot cell disruption system (Constant Systems, Ltd). Clarified lysates were immunoprecipitated overnight at 4°C using ANTI-FLAG M2 affinity gel (Sigma) ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were passed one time through a French press (Constant Systems LTD) at 4°C with 2.2 kpsi of pressure ...
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bioRxiv - Microbiology 2022Quote: ... and lysed twice using a One Shot cell disrupter (Constant Systems, Inc.) at 27 kpsi and 4 °C ...
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bioRxiv - Biochemistry 2024Quote: ... 5mM β-mercaptoethanol) and homogenized utilizing One Shot Cell Disruptor (Constant Systems) operating at 1.0 kbar ...
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bioRxiv - Microbiology 2022Quote: ... Cells were lysed by passage through a One-Shot cell disrupter (Constant Systems) at 0.83 kbar ...
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bioRxiv - Plant Biology 2021Quote: ... and lysed using the One Shot constant cell disruption system (Constant Systems Limited, U.K.) at 2.4□kbar ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The resuspended cells were lysed using the One-Shot Cell Disrupter (Constant Systems, UK) following which the lysate was incubated for 30 minutes at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... The resuspended cells were ruptured by the One-Shot Cell Disruptor (Constant Systems, UK) and the lysate was incubated for 1 hour shaking at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were lysed at 30 kpsi using the One-Shot cell lyser (Constant Systems Ltd.) and upon addition of EDTA-free cOmplete™ protease inhibitor cocktail (Roche) ...
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bioRxiv - Microbiology 2023Quote: ... and lysed using the One Shot Constant Cell Disruption System (Constant Systems Limited, United Kingdom) at 2.4 kbar ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were harvested by centrifugation and lysed by One shot cell disrupter (Constant Systems Ltd.) with a pressure of 2 kbar in lysis buffer (1 M NaCl ...
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bioRxiv - Microbiology 2023Quote: ... cells were homogenized using a cell homogenizer at 30kpsi (One Shot Model; Constant Systems LTD).
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bioRxiv - Molecular Biology 2024Quote: ... The cells were disrupted at 20 kPsi (one shot) in Cell Disruptor (Constant Systems Ltd.) and centrifuged (20000 × g ...
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bioRxiv - Biochemistry 2019Quote: ... Cells were lysed using a cell disruptor in one-shot mode (Constant Systems, Daventry, United Kingdom) and lysates were clarified by spinning for 30 min at 16,000 RPM in a Sorvall SS-34 rotor at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... Cells were lysed using a cell disruptor in one-shot mode (Constant Systems, Daventry, United Kingdom) and lysates were clarified by spinning for 30 min at 16,000 RPM in a Sorvall SS-34 rotor at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... Cells were lysed using a cell disruptor in one-shot mode (Constant Systems, Daventry, United Kingdom) and lysates were clarified by spinning for 30 min at 16,000 RPM in a Sorvall SS-34 rotor at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... Cells were lysed using a cell disruptor in one-shot mode (Constant Systems, Daventry, United Kingdom) and lysates were clarified by spinning for 30 min at 16,000 RPM in a Sorvall SS-34 rotor at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Cells in this resuspension were lysed by two passes through a One-Shot cell disruptor (Constant Systems) at 23 kpsi and the cell debris was removed by centrifugation (40,000 x g at 4°C for 30 min) ...
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bioRxiv - Physiology 2023Quote: ... cells were lysed at 19 kPa using a cell disruption one-shot (OS) system (Constant Systems LTD). 100 µL of the cell lysate (equivalent to approximately 40 million cells ...
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bioRxiv - Biochemistry 2024Quote: ... 2 mM β-mercaptoethanol) and disrupted by high pressure using a One Shot Cell Disruptor (Constant Systems) at 1.9 kbar ...
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Uptake of monoaromatic hydrocarbons during biodegradation by FadL channel-mediated lateral diffusionbioRxiv - Microbiology 2020Quote: ... pH 8) and ruptured by one passage at 23,000 psi through a cell disrupter (Constant Systems 0.75 kW). Total membranes were obtained by ultracentrifugation in a Beckman 45Ti rotor for 45 mins at 42,000 rpm at 4°C ...
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Uptake of monoaromatic hydrocarbons during biodegradation by FadL channel-mediated lateral diffusionbioRxiv - Microbiology 2020Quote: ... pH 8] and ruptured by one passage at 23,000 psi through a cell disrupter (Constant Systems 0.75 kW). Inclusion bodies and cell debris were collected by centrifugation at 17,000 × g for 20 mins ...
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bioRxiv - Microbiology 2021Quote: ... Cells were broken by one pass through a cell disruptor (Constant Systems 0.75 kW operated at 23 kpsi), centrifuged at 42,000 rpm for 45 minutes at 4 °C (45Ti rotor ...
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Mechanism of the ANT-mediated transport of fatty acid anions across the inner mitochondrial membranebioRxiv - Biophysics 2022Quote: ... To isolate inclusion bodies we centrifuged cells disrupted by high-pressure cell disrupter One Shot (Constant Systems Limited, Daventry, UK) at 1 kbar ...
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bioRxiv - Microbiology 2020Quote: Cells containing pET19b-Rv97 were re-suspended in Buffer C (52) plus 0.5 mM PMSF and lysed in a One Shot Disrupter (Constant Systems, Inc.). After centrifugation (21,000 x g ...
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bioRxiv - Biochemistry 2022Quote: ... Cell pellets containing different subcomplexes were mixed in defined ratio (see below) and lysed at 4°C with a one-shot cell disruptor (Constant Systems). The lysate was clarified by centrifugation at 30 000 g for 30 min at 18°C ...
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bioRxiv - Microbiology 2022Quote: ... Thawed cell pellets were supplemented with an additional 1 mM PMSF and lysed using a One Shot homogeniser (Constant Systems Ltd) at 20 KPSI at 4°C and then centrifuged at 19 000 RPM in a JA-25.50 rotor (Beckman Coulter ...
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bioRxiv - Microbiology 2020Quote: ... Bacterial pellets were resuspended in 1/50th of the original culture volume and lyzed using a cell disruptor (One shot model, Constant System Inc.). Proteins were purified by affinity chromatography using a GSTrap HP affinity column (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... at 4°C and loaded 3 times in a CellD disrupter (Constant Systems). The lysate was centrifuged for 15min at 12000 x g at 4°C to remove cell debris and the supernatant was centrifuged again for 1h at 100000 x g at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... The bacteria were harvested by centrifugation and lysed using a One-Shot cell disrupter following the manufacturer’s instructions (Constant Systems Ltd., Daventry, United Kingdom). Cell lysates were cleared by centrifugation ...
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bioRxiv - Microbiology 2023Quote: ... Cell lysis was performed by 3 successive passages in a cell disrupter (Constant Systems Ltd) at 2.5 kbar pressure ...
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bioRxiv - Microbiology 2023Quote: ... and lysed by 3 passages at 2.5 kBar using a cell disrupter (Constant Systems Ltd). Clarification of the lysate was then performed by two centrifugations of the extract at 22,000 g for 20 min at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were harvested 2-3 hours later and lysed using a cell disrupter (Constant Systems Ltd.). Proteins were purified from inclusion bodies using Nickel affinity chromatography on prepacked HisTrap FF columns (Cytiva ...