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Citations for Constant Systems :
1 - 19 of 19 citations for 6H Furo 2 3 g 3 benzazepine 2 ethyl 7 8 9 10 tetrahydro 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Cells were harvested 2-3 hours later and lysed using a cell disrupter (Constant Systems Ltd.). Proteins were purified from inclusion bodies using Nickel affinity chromatography on prepacked HisTrap FF columns (Cytiva ...
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bioRxiv - Microbiology 2023Quote: ... at 4°C and loaded 3 times in a CellD disrupter (Constant Systems). The lysate was centrifuged for 15min at 12000 x g at 4°C to remove cell debris and the supernatant was centrifuged again for 1h at 100000 x g at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... The cell suspensions were incubated with DNase I (50 μg ml-1) and MgCl2 (2 mM) for 10 min at room temperature before extraction with a continuous-flow cell disruptor (Constant Systems) at 277 K and a pressure between 17-18 KPSI ...
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bioRxiv - Microbiology 2023Quote: ... Cell lysis was performed by 3 successive passages in a cell disrupter (Constant Systems Ltd) at 2.5 kbar pressure ...
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bioRxiv - Microbiology 2023Quote: ... and lysed by 3 passages at 2.5 kBar using a cell disrupter (Constant Systems Ltd). Clarification of the lysate was then performed by two centrifugations of the extract at 22,000 g for 20 min at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... cracked in a cell disruptor (Constant Systems; 2 cycles at 25 kpsi), and clarified by centrifugation (45 min ...
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bioRxiv - Microbiology 2023Quote: ... Cells were harvested by centrifugation (6,000 g, 10 min, 4°C) and lysed using a French-press (Constant Systems) at 36,000 ps ...
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bioRxiv - Biophysics 2023Quote: ... 2 mM CaCl2 through a French press cell disrupter at 25,000 psi (Constant Systems Ltd.) followed by centrifugation at 20,000 x g where the clear lysate was subsequently loaded onto a 5 mL Ni-NTA Sepharose column (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... 2 mM β-mercaptoethanol) and disrupted by high pressure using a One Shot Cell Disruptor (Constant Systems) at 1.9 kbar ...
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bioRxiv - Molecular Biology 2022Quote: ... pH 8) using a Celldisrupter TS 0.75 (Constant Systems) at 1350 bar.
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bioRxiv - Plant Biology 2020Quote: ... 5 mM EDTA before being lysed at 2°C with an automatic cell pressure lysis machine at 0.3 KBar (Constant Systems) in homogenization buffer (50 mM Tris adjusted to pH 8.0 with MES ...
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bioRxiv - Plant Biology 2021Quote: ... in the presence of 5 mM EDTA and lysed by 1-2 passes through a cell disrupter operated at 35-37 kpsi (TS-Series 0.75 kW; Constant Systems). Membranes were collected from the suspension by centrifugation at 200,000 x g for 90 mins (45Ti rotor ...
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bioRxiv - Plant Biology 2022Quote: ... Cells were resuspended in phosphate buffer pH 7.0 (2 mL for 1 mL pellet) containing 300 μl of protease inhibitor 1x (Constant systems Ltd. United Kingdom).
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Uptake of monoaromatic hydrocarbons during biodegradation by FadL channel-mediated lateral diffusionbioRxiv - Microbiology 2020Quote: ... pH 8) and ruptured by one passage at 23,000 psi through a cell disrupter (Constant Systems 0.75 kW). Total membranes were obtained by ultracentrifugation in a Beckman 45Ti rotor for 45 mins at 42,000 rpm at 4°C ...
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Uptake of monoaromatic hydrocarbons during biodegradation by FadL channel-mediated lateral diffusionbioRxiv - Microbiology 2020Quote: ... pH 8] and ruptured by one passage at 23,000 psi through a cell disrupter (Constant Systems 0.75 kW). Inclusion bodies and cell debris were collected by centrifugation at 17,000 × g for 20 mins ...
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bioRxiv - Biophysics 2024Quote: ... 10% glycerol) using a continuous flow cell disruptor (Constant Systems Ltd.). Lysate was centrifuged at 15.000xg for 30 min ...
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bioRxiv - Molecular Biology 2020Quote: ... 15 mM Imidazole (pH 7.8) and 10% glycerol) using a Constant Cell disruptor (Constant Systems) with a pressure of 25 kPsi ...
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bioRxiv - Biophysics 2022Quote: ... the cells were resuspended in 50 mM sodium phosphate buffer (pH 7.4) supplemented with 300 mM NaCl and 10% glycerol and lysed in the French press (Constant systems). Then the proteins were purified from the lysate with the Ni2+-NTA column of ÄKTA Pure (GE healthcare) ...
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bioRxiv - Cell Biology 2023Quote: ... The cell pellets were resuspended in 50 mM sodium phosphate buffer (pH 7.4) supplemented with 300 mM NaCl and 10% glycerol and lysed by cell homogenization (Constant systems). Then the proteins were purified from the cell lysate through Ni2+-NTA affinity column (ÄKTA Pure ...