Labshake search
Citations for Constant Systems :
1 - 21 of 21 citations for 6 tert Butyl 4 chlorothieno 2 3 d pyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... at 4°C and loaded 3 times in a CellD disrupter (Constant Systems). The lysate was centrifuged for 15min at 12000 x g at 4°C to remove cell debris and the supernatant was centrifuged again for 1h at 100000 x g at 4°C ...
-
bioRxiv - Biochemistry 2021Quote: ... Cells were harvested 2-3 hours later and lysed using a cell disrupter (Constant Systems Ltd.). Proteins were purified from inclusion bodies using Nickel affinity chromatography on prepacked HisTrap FF columns (Cytiva ...
-
bioRxiv - Biophysics 2021Quote: ... and the cells broken with a TS5/48/AE/6□A cell disrupter (Constant Systems) at 31,000□psi at 4°C ...
-
bioRxiv - Microbiology 2023Quote: ... Cell lysis was performed by 3 successive passages in a cell disrupter (Constant Systems Ltd) at 2.5 kbar pressure ...
-
bioRxiv - Microbiology 2023Quote: ... and lysed by 3 passages at 2.5 kBar using a cell disrupter (Constant Systems Ltd). Clarification of the lysate was then performed by two centrifugations of the extract at 22,000 g for 20 min at 4°C ...
-
bioRxiv - Microbiology 2023Quote: ... pH 7.6 sodium phosphate buffer (supplemented with 300 mM NaCl and DNase) and lysed using the high-pressure cell disrupter (Constant Systems), by running the sample twice under 1.9 kbar with subsequent cell fractionation using ultracentrifugation ...
-
bioRxiv - Biochemistry 2021Quote: ... Cells were broken with a cell disrupter with 4 cycles at 35 kPsi (Constant Systems) and cellular debris was removed by centrifugation at 90 000 x g for 20 min at 4°C (45 Ti rotor ...
-
bioRxiv - Cell Biology 2022Quote: ... cracked in a cell disruptor (Constant Systems; 2 cycles at 25 kpsi), and clarified by centrifugation (45 min ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were harvested by centrifugation (6,000 g, 10 min, 4°C) and lysed using a French-press (Constant Systems) at 36,000 ps ...
-
bioRxiv - Biophysics 2023Quote: ... 2 mM CaCl2 through a French press cell disrupter at 25,000 psi (Constant Systems Ltd.) followed by centrifugation at 20,000 x g where the clear lysate was subsequently loaded onto a 5 mL Ni-NTA Sepharose column (GE Healthcare ...
-
bioRxiv - Biochemistry 2023Quote: ... 30 mM Imidazole and 0.02 mg/ml DNase I and broken with a Constant cell disruptor at 4 °C and 25 kPsi (Constant Systems). The cell lysate was clarified by centrifugation for 30 minutes at 30,000 x g ...
-
bioRxiv - Microbiology 2021Quote: ... cells were resuspended in lysis buffer (see below) at 4°C before lysis using a TS series cell disruptor (Constant Systems) at 24 kpsi ...
-
bioRxiv - Microbiology 2022Quote: ... cells were resuspended in lysis buffer (see below) at 4°C before lysis using a TS series cell disruptor (Constant Systems) at 24 kpsi ...
-
bioRxiv - Biochemistry 2022Quote: ... Cell pellets containing different subcomplexes were mixed in defined ratio (see below) and lysed at 4°C with a one-shot cell disruptor (Constant Systems). The lysate was clarified by centrifugation at 30 000 g for 30 min at 18°C ...
-
bioRxiv - Biochemistry 2024Quote: ... in a ratio of 7g/100 ml and lysed at 4 °C and 25 kPsi using a Cell Disruptor (Constant System). Cell debris was removed by centrifugation for 30 minutes at 13,000 × g ...
-
bioRxiv - Biochemistry 2024Quote: ... 2 mM β-mercaptoethanol) and disrupted by high pressure using a One Shot Cell Disruptor (Constant Systems) at 1.9 kbar ...
-
bioRxiv - Molecular Biology 2023Quote: ... cell pellets were lysed at 4°C using high-pressure homogenization at 25 Kpsi (1 Kpsi = 69 _ 103 MPa) (Constant System Ltd, UK) in lysis buffer [50 mM Tris-HCl pH:7.5 ...
-
bioRxiv - Plant Biology 2020Quote: ... 5 mM EDTA before being lysed at 2°C with an automatic cell pressure lysis machine at 0.3 KBar (Constant Systems) in homogenization buffer (50 mM Tris adjusted to pH 8.0 with MES ...
-
bioRxiv - Plant Biology 2021Quote: ... in the presence of 5 mM EDTA and lysed by 1-2 passes through a cell disrupter operated at 35-37 kpsi (TS-Series 0.75 kW; Constant Systems). Membranes were collected from the suspension by centrifugation at 200,000 x g for 90 mins (45Ti rotor ...
-
bioRxiv - Microbiology 2021Quote: ... The cell suspensions were incubated with DNase I (50 μg ml-1) and MgCl2 (2 mM) for 10 min at room temperature before extraction with a continuous-flow cell disruptor (Constant Systems) at 277 K and a pressure between 17-18 KPSI ...
-
bioRxiv - Plant Biology 2022Quote: ... Cells were resuspended in phosphate buffer pH 7.0 (2 mL for 1 mL pellet) containing 300 μl of protease inhibitor 1x (Constant systems Ltd. United Kingdom).