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Citations for Constant Systems :
1 - 29 of 29 citations for 6 1 2 4 TRIAZOL 1YL PURINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... in the presence of 5 mM EDTA and lysed by 1-2 passes through a cell disrupter operated at 35-37 kpsi (TS-Series 0.75 kW; Constant Systems). Membranes were collected from the suspension by centrifugation at 200,000 x g for 90 mins (45Ti rotor ...
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bioRxiv - Molecular Biology 2023Quote: ... cell pellets were lysed at 4°C using high-pressure homogenization at 25 Kpsi (1 Kpsi = 69 _ 103 MPa) (Constant System Ltd, UK) in lysis buffer [50 mM Tris-HCl pH:7.5 ...
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bioRxiv - Biophysics 2021Quote: ... and the cells broken with a TS5/48/AE/6□A cell disrupter (Constant Systems) at 31,000□psi at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... The cell suspensions were incubated with DNase I (50 μg ml-1) and MgCl2 (2 mM) for 10 min at room temperature before extraction with a continuous-flow cell disruptor (Constant Systems) at 277 K and a pressure between 17-18 KPSI ...
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bioRxiv - Plant Biology 2022Quote: ... Cells were resuspended in phosphate buffer pH 7.0 (2 mL for 1 mL pellet) containing 300 μl of protease inhibitor 1x (Constant systems Ltd. United Kingdom).
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bioRxiv - Microbiology 2023Quote: ... at 4°C and loaded 3 times in a CellD disrupter (Constant Systems). The lysate was centrifuged for 15min at 12000 x g at 4°C to remove cell debris and the supernatant was centrifuged again for 1h at 100000 x g at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... pH 7.6 sodium phosphate buffer (supplemented with 300 mM NaCl and DNase) and lysed using the high-pressure cell disrupter (Constant Systems), by running the sample twice under 1.9 kbar with subsequent cell fractionation using ultracentrifugation ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were broken with a cell disrupter with 4 cycles at 35 kPsi (Constant Systems) and cellular debris was removed by centrifugation at 90 000 x g for 20 min at 4°C (45 Ti rotor ...
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bioRxiv - Cell Biology 2022Quote: ... cracked in a cell disruptor (Constant Systems; 2 cycles at 25 kpsi), and clarified by centrifugation (45 min ...
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bioRxiv - Microbiology 2023Quote: ... Cells were harvested by centrifugation (6,000 g, 10 min, 4°C) and lysed using a French-press (Constant Systems) at 36,000 ps ...
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bioRxiv - Biophysics 2023Quote: ... 2 mM CaCl2 through a French press cell disrupter at 25,000 psi (Constant Systems Ltd.) followed by centrifugation at 20,000 x g where the clear lysate was subsequently loaded onto a 5 mL Ni-NTA Sepharose column (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... 30 mM Imidazole and 0.02 mg/ml DNase I and broken with a Constant cell disruptor at 4 °C and 25 kPsi (Constant Systems). The cell lysate was clarified by centrifugation for 30 minutes at 30,000 x g ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were harvested 2-3 hours later and lysed using a cell disrupter (Constant Systems Ltd.). Proteins were purified from inclusion bodies using Nickel affinity chromatography on prepacked HisTrap FF columns (Cytiva ...
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bioRxiv - Microbiology 2021Quote: ... cells were resuspended in lysis buffer (see below) at 4°C before lysis using a TS series cell disruptor (Constant Systems) at 24 kpsi ...
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bioRxiv - Microbiology 2022Quote: ... cells were resuspended in lysis buffer (see below) at 4°C before lysis using a TS series cell disruptor (Constant Systems) at 24 kpsi ...
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bioRxiv - Biochemistry 2022Quote: ... Cell pellets containing different subcomplexes were mixed in defined ratio (see below) and lysed at 4°C with a one-shot cell disruptor (Constant Systems). The lysate was clarified by centrifugation at 30 000 g for 30 min at 18°C ...
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bioRxiv - Biochemistry 2024Quote: ... in a ratio of 7g/100 ml and lysed at 4 °C and 25 kPsi using a Cell Disruptor (Constant System). Cell debris was removed by centrifugation for 30 minutes at 13,000 × g ...
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bioRxiv - Biochemistry 2024Quote: ... 2 mM β-mercaptoethanol) and disrupted by high pressure using a One Shot Cell Disruptor (Constant Systems) at 1.9 kbar ...
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bioRxiv - Plant Biology 2020Quote: ... 5 mM EDTA before being lysed at 2°C with an automatic cell pressure lysis machine at 0.3 KBar (Constant Systems) in homogenization buffer (50 mM Tris adjusted to pH 8.0 with MES ...
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bioRxiv - Biophysics 2022Quote: ... 1 mM DTT) and cells lysed by 1 cycle of French Press at 25 kPsi (Constant Systems Ltd MC Cell Disrupter). The lysate was centrifuged at 4 °C and 20000 g for 30 min and the supernatant applied to a gravity flow column with 4 mL pre-equilibrated Ni-NTA Sepharose resin (GE Healthcare) ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM TCEP and protease inhibitors) and lysed using ultrasonic homogenizer (Constant Systems Ltd). The proteins were first purified by affinity chromatography using TALON metal affinity resin (TaKaRa) ...
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bioRxiv - Microbiology 2023Quote: ... 1% NP-40) and then disrupted by French press system (Constant Systems Ltd, Daventry, UK). The lysed cells were centrifuged at 12,000g ...
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bioRxiv - Molecular Biology 2021Quote: ... 5% glycerol) supplemented with 1 mM of PMSF and disrupted using Cell Disruptor CF (Constant Systems). The lysate was cleared by centrifugation ...
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bioRxiv - Biochemistry 2020Quote: ... and 1 mM MgSO4) before lysing them by three passages through a Constant cell disruptor (Constant Systems) at 39 000 psi ...
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bioRxiv - Neuroscience 2020Quote: ... 1 mM PMSF) and lysed by cell disruption (Constant systems LTD 0.75KW, two passes with 25K Psi) at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... 1 mM β-mercaptoethanol (pH 7.5)) and lysed with a cell disruptor (Constant Systems, Daventry Northants, UK)) ...
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bioRxiv - Biochemistry 2022Quote: ... 1 mM β-mercaptoethanol (Buffer A) and lysed with a cell disruptor (Constant Systems, Daventry Northants, UK). The supernatant was loaded onto HisTrapTM HP 5 ml column (GE healthcare ...
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bioRxiv - Microbiology 2022Quote: ... Thawed cell pellets were supplemented with an additional 1 mM PMSF and lysed using a One Shot homogeniser (Constant Systems Ltd) at 20 KPSI at 4°C and then centrifuged at 19 000 RPM in a JA-25.50 rotor (Beckman Coulter ...
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bioRxiv - Microbiology 2020Quote: ... Bacterial pellets were resuspended in 1/50th of the original culture volume and lyzed using a cell disruptor (One shot model, Constant System Inc.). Proteins were purified by affinity chromatography using a GSTrap HP affinity column (GE Healthcare ...