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Citations for Constant Systems :
1 - 13 of 13 citations for 5 NITRO BENZO B THIOPHENE 2 CARBOXYLIC ACID METHYL ESTER since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... cracked in a cell disruptor (Constant Systems; 2 cycles at 25 kpsi), and clarified by centrifugation (45 min ...
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bioRxiv - Molecular Biology 2021Quote: ... 5% glycerol) supplemented with 1 mM of PMSF and disrupted using Cell Disruptor CF (Constant Systems). The lysate was cleared by centrifugation ...
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bioRxiv - Biophysics 2023Quote: ... 2 mM CaCl2 through a French press cell disrupter at 25,000 psi (Constant Systems Ltd.) followed by centrifugation at 20,000 x g where the clear lysate was subsequently loaded onto a 5 mL Ni-NTA Sepharose column (GE Healthcare ...
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bioRxiv - Plant Biology 2021Quote: ... The cells were disrupted at 5 kpsi once using the MC Cell Disruptor (Constant Systems Ltd, Northants, UK). The disrupted cells (0.5 mg Chl in 4 mL ...
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bioRxiv - Biophysics 2023Quote: ... 5 mM ý-mercaptoethanol (bME)) using a French pressure cell disrupter (25 kpsi; Constant Systems Ltd, Daventry, UK), and the lysate was cleared by centrifugation at 20,000xg for 45 min at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were harvested 2-3 hours later and lysed using a cell disrupter (Constant Systems Ltd.). Proteins were purified from inclusion bodies using Nickel affinity chromatography on prepacked HisTrap FF columns (Cytiva ...
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bioRxiv - Biochemistry 2024Quote: ... 2 mM β-mercaptoethanol) and disrupted by high pressure using a One Shot Cell Disruptor (Constant Systems) at 1.9 kbar ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were resuspended in membrane resuspension buffer (75mM Tris pH 8.0, 150 mM NaCl, and 5% glycerol) and then lysed using a cell disruptor (Constant Systems Ltd) at 28 ...
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bioRxiv - Biochemistry 2021Quote: ... 5% glycerol and lyzed at a pressure of 2400 bar in a CF high-pressure homogenizer (Constant Systems Ltd., Daventry United Kingdom). The total extract of proteins was then centrifuged for 20 min at 20,000 g and the soluble fraction loaded on an affinity chromatography with 2 ml of NiNTA resin (Sigma-Aldrich Merck ...
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bioRxiv - Plant Biology 2020Quote: ... 5 mM EDTA before being lysed at 2°C with an automatic cell pressure lysis machine at 0.3 KBar (Constant Systems) in homogenization buffer (50 mM Tris adjusted to pH 8.0 with MES ...
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bioRxiv - Plant Biology 2021Quote: ... in the presence of 5 mM EDTA and lysed by 1-2 passes through a cell disrupter operated at 35-37 kpsi (TS-Series 0.75 kW; Constant Systems). Membranes were collected from the suspension by centrifugation at 200,000 x g for 90 mins (45Ti rotor ...
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bioRxiv - Microbiology 2021Quote: ... The cell suspensions were incubated with DNase I (50 μg ml-1) and MgCl2 (2 mM) for 10 min at room temperature before extraction with a continuous-flow cell disruptor (Constant Systems) at 277 K and a pressure between 17-18 KPSI ...
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bioRxiv - Plant Biology 2022Quote: ... Cells were resuspended in phosphate buffer pH 7.0 (2 mL for 1 mL pellet) containing 300 μl of protease inhibitor 1x (Constant systems Ltd. United Kingdom).