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1 - 10 of 10 citations for Primary Human Cell since 2019

• Cholesterol metabolism drives regulatory B cell function

  Jack A. Bibby, et al., bioRxiv - Immunology 2020

  Quote: ... Human: Peripheral blood mononuclear cells were isolated by density gradient centrifugation using Lymphoprep (Alere Technologies). Fresh CD19+ B cells and CD4+ T cells were subsequently isolated by magnetic cell sorting ...
• Glycan-specific IgM is critical for human immunity to Staphylococcus aureus

  Astrid Hendriks, et al., bioRxiv - Microbiology 2023

  Quote: ... Human neutrophils were freshly isolated using Polymorphprep (Alere technologies) per manufacturer instructions ...
• Streptococcus pneumoniae evades host cell phagocytosis and limits host mortality through its cell wall anchoring protein PfbA

  Masaya Yamaguchi, et al., bioRxiv - Microbiology 2019

  Quote: ... Human neutrophils and monocytes were prepared using Polymorphprep (Alere Technologies AS, Oslo, Norway), according to the manufacturer’s instructions ...
• COVID-19 patients upregulate toll-like receptor 4-mediated inflammatory signaling that mimics bacterial sepsis

  Kyung Mok Sohn, et al., bioRxiv - Immunology 2020

  Quote: Human PBMCs from healthy volunteers were isolated from heparinized venous blood using Ficoll-Hypaque (Lymphoprep; Alere technologies, Oslo, Norway) as described previously (37) ...
• Involvement of ILC1-like innate lymphocytes in human autoimmunity, lessons from alopecia areata

  Rimma Laufer Britva, et al., bioRxiv - Immunology 2022

  Quote: Autologous human PBMCs were isolated from heparinized whole blood from healthy donors by Lymphoprep density gradient centrifugation (Alere Technologies, Norway). Cells were frozen for further assays (70% FBS ...
• Siglec-1 on dendritic cells mediates SARS-CoV-2 trans-infection of target cells while on macrophages triggers proinflammatory responses

  Daniel Perez-Zsolt, et al., bioRxiv - Microbiology 2021

  Quote: Peripheral blood mononuclear cells were obtained with a Ficoll-Hypaque gradient (Alere Technologies AS) from blood donors and monocyte populations (>90% CD14+ ...
• Anthrax intoxication reveals that ER-Golgi membrane contact sites control the formation of plasma membrane lipid nanodomains

  Muhammad U. Anwar, et al., bioRxiv - Cell Biology 2022

  Quote: DRMs were prepared from RPE1 cells as described previously using OptiPrep (Alere Technologies #1114542) gradients as previously published (Abrami et al. ...
• Actin-regulated Siglec-1 nanoclustering influences HIV-1 capture and virus-containing compartment formation in dendritic cells

  Enric Gutiérrez-Martínez, et al., bioRxiv - Cell Biology 2022

  Quote: Peripheral blood mononuclear cells (PBMC) were obtained with a Ficoll-Hypaque gradient (Alere Technologies AS) from HIV-1-seronegative donors ...
• Simultaneous inhibition of human CD4 and 4-1BB biogenesis suppresses cytotoxic T lymphocyte proliferation

  Elisa Claeys, et al., bioRxiv - Immunology 2021

  Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from buffy coats (Red Cross Belgium) by density gradient centrifugation using Lymphoprep (Alere Technologies AS) and HetaSep (STEMCELL Technologies ...
• Early steps of multi-receptor viral interactions dissected by high-density, multi-color single molecule mapping in living cells

  Nicolas Mateos, et al., bioRxiv - Biophysics 2023

  Quote: Human immature dendritic cells (imDCs) were obtained from peripheral blood mononuclear cells (PBMC) from HIV-1 seronegative donors using a Ficoll-Hypaque gradient (Alere Technologies AS). The monocyte population was selected by adherence on a T75cm2 flask for 1 hour ...