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Citations for Sanquin :
1 - 43 of 43 citations for Dengue Virus Serotype 3 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... CHIKV virus stock (5 × 108 TCID50/mL) and full human blood (Sanquin) was 1:2 mixed to make the infectious blood meal with a final virus titer of 1.7× 108 TCID50/ml ...
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bioRxiv - Microbiology 2021Quote: ... and 3-5% human blood type O red blood cells (RBCs) (Sanquin, the Netherlands) at 37°C in 3% O2 ...
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bioRxiv - Immunology 2020Quote: 10×106 cells/ml CD4+CD45RA+ T cells were cultured for 3 days in the presence of 1.5 µg/ml plate bound αCD3 (CLB-T3/4.E, 1XE Sanquin) and 1 µg/ml soluble αCD28 (CLB-CD28/1 ...
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bioRxiv - Immunology 2020Quote: ... Anti-RBD and anti-NP IgG antibodies were measured in EDTA plasma samples at 100-1200 fold dilutions using ELISA as previously described.11 Plates were coated with RBD or N protein and specific IgG antibodies were detected using anti-human IgG (MH16, Sanquin).
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bioRxiv - Immunology 2019Quote: Human T cells were cultured in T-cell mixed media (Miltenyi) supplemented with 5% heat-inactivated human serum (Sanquin) and fetal bovine serum (FBS ...
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bioRxiv - Immunology 2023Quote: ... Treg cells (105 cells/well) were added and the medium was supplemented with soluble anti-CD3ε [25] (T3/4.E, Sanquin). T3/4.E is able to stimulate Tresp cells directly in soluble form [25] ...
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bioRxiv - Cancer Biology 2021Quote: ... Peripheral blood mononuclear cells (PBMCs) were obtained from Sanquin Blood bank (Amsterdam ...
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bioRxiv - Immunology 2020Quote: ... T cell culture medium consisted of 5% human serum (Sanquin), 5% FCS ...
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bioRxiv - Immunology 2022Quote: ... PB-NK cells were obtained from buffy coats of healthy donors (Sanquin) after written informed consent according to the Declaration of Helsinki and all experimental protocols concur to institutional guidelines ...
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bioRxiv - Immunology 2022Quote: ... The cells were thawed in medium with 71% Human Serum (HS; Sanquin), 0.03% DNAse and 0.1%MgCL2 and were washed (at 300 g for 15 minutes ...
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bioRxiv - Immunology 2022Quote: ... Antigen-specific T cells were detected using PE-conjugated s598 tetramers (Sanquin Reagents) after an incubation of 20 min at 37 °C ...
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bioRxiv - Bioengineering 2020Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from blood of healthy donors (Sanquin) using standard Ficoll (GE Healthcare ...
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bioRxiv - Bioengineering 2022Quote: Peripheral blood mononuclear cells (PBMCs) were isolated from human peripheral blood buffy coats (Sanquin, Eindhoven ...
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The tumour microenvironment shapes dendritic cell plasticity in a human organotypic melanoma culturebioRxiv - Immunology 2019Quote: Peripheral blood mononuclear cells (PBMCs) were isolated from buffy coats obtained from healthy volunteers (Sanquin) and purified via centrifugation over a Ficoll density gradient (Axis-Shield ...
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bioRxiv - Immunology 2020Quote: Peripheral blood mononuclear cells (PBMCs) were obtained from whole blood of healthy donors (from Sanquin Institute Amsterdam or from GSK Stevenage Blood Donation Unit ...
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bioRxiv - Cancer Biology 2020Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from healthy donor buffy coats (Sanquin, Amsterdam, the Netherlands) by density gradient centrifugation using Lymphoprep (Stem cell technologies ...
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bioRxiv - Cancer Biology 2024Quote: Peripheral blood mononuclear cells (PBMCs) were isolated from buffy coats obtained from healthy blood donors (Sanquin). Written informed consent for research use of donated blood was obtained by the Sanquin blood bank ...
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bioRxiv - Cell Biology 2019Quote: Dendritic cells were derived from peripheral blood monocytes isolated from a buffy coat (Sanquin, Nijmegen, The Netherlands) (de Vries et al. ...
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bioRxiv - Cell Biology 2023Quote: Dendritic cells were derived from peripheral blood monocytes isolated from a buffy coat (Sanquin, Nijmegen, The Netherlands) (de Vries et al ...
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bioRxiv - Immunology 2023Quote: Dendritic cells were derived from peripheral blood monocytes isolated from a buffy coat (Sanquin, Nijmegen, The Netherlands) 37 ...
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bioRxiv - Immunology 2023Quote: Cytokines in cell-free supernatants were measured using enzyme-linked immunosorbent assay (ELISA) for IL-6 (Sanquin), TNF-α (Diaclone ...
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bioRxiv - Cell Biology 2023Quote: Peripheral blood mononuclear cells (PMBCs) were isolated from buffy coats obtained from healthy donors (Sanquin, The Netherlands) by density gradient centrifugation with Ficoll-Paque PLUS (GE Healthcare) ...
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bioRxiv - Immunology 2024Quote: ... Human healthy donor peripheral blood mononuclear cells (PBMC) were isolated from buffy coats (Sanquin, Amsterdam, the Netherlands) using Ficoll-Paque according to the manufactureŕs protocol ...
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bioRxiv - Immunology 2023Quote: Human cDC2s were isolated from peripheral blood mononuclear cells derived from healthy individuals (Sanquin, Nijmegen, the Netherlands) using the MACS CD1c+ isolation kit (130-119-475 ...
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bioRxiv - Genomics 2021Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from buffy coats from healthy volunteers (Sanquin Bloodbank, Nijmegen, the Netherlands). Isolation was performed using Ficoll-Paque ...
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bioRxiv - Bioengineering 2022Quote: Peripheral blood mononuclear cells (PBMCs) were isolated from a human peripheral blood buffy coat (Sanquin, Eindhoven, The Netherlands) of one healthy donor after written informed consent per the Declaration of Helsinki and according to the institutional guidelines ...
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bioRxiv - Immunology 2020Quote: ... CD4+CD45RA+ T cells were activated for 5-6 days with 1.5 µg/ml plate bound αCD3 (Sanquin) and 1 µg/ml soluble αCD28 (Sanquin ...
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bioRxiv - Microbiology 2021Quote: ... Fresh human serum and human red blood cells (RBC) were obtained from the Dutch National Blood Bank (Sanquin Amsterdam ...
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bioRxiv - Cell Biology 2023Quote: Peripheral blood mononuclear cells (PBMCs) were isolated from human peripheral blood buffy coats of two healthy donors (Sanquin, Eindhoven ...
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bioRxiv - Bioengineering 2019Quote: Human peripheral blood mononuclear cells (hPBMCs) were isolated from two different buffy coats of healthy donors (purchased from Sanquin Blood Supply Foundation ...
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bioRxiv - Immunology 2022Quote: ... Cells were expanded using agonistic mAbs against CD3 (clone CLB-T3/4.E, IgE isotype, Sanquin, 0.1 μg/ml) and CD28 (clone CLB-CD28/1 ...
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bioRxiv - Immunology 2019Quote: Peripheral mononuclear blood cells (PBMC) were isolated from peripheral blood or buffy coats of healthy individuals collected by Sanquin Blood Supply (Amsterdam ...
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bioRxiv - Genomics 2024Quote: ... peripheral blood mononuclear cells (PBMCs) were isolated from buffy coats of anonymous blood bank donors (Sanquin, Amsterdam, The Netherlands) by Ficoll paque (Apotheek LUMC ...
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bioRxiv - Genomics 2024Quote: ... peripheral blood mononuclear cells (PBMCs) were isolated from buffy coats of anonymous blood bank donors (Sanquin, Amsterdam, The Netherlands) by Ficoll paque (Apotheek LUMC ...
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bioRxiv - Microbiology 2020Quote: ... Fresh human serum and red blood cells (RBC) for these experiments were obtained from the Dutch National Blood Bank (Sanquin Amsterdam ...
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bioRxiv - Cancer Biology 2022Quote: ... 2-3 wells containing 0.5-1 x106 live cells from the tissue digest were cultured for 10-13 days in 24 wells plates in 20/80 T-cell mixed media (Miltenyi) containing 5% human serum (HS) (Sanquin), 5% FBS ...
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bioRxiv - Immunology 2019Quote: ... 2-3 wells containing 0,5-1*106 live cells from the tissue digest were cultured for 10-13 days in 24 wells plates in 20/80 T-cell mixed media (Miltenyi) containing 5% human serum (HS) (Sanquin), 5% FBS ...
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bioRxiv - Immunology 2024Quote: 1-3×106 cells were cultured in 24-well plates at 37°C and 5% CO2 in 20/80 T-cell mixed media (Miltenyi) containing 5% human serum (HS) (Sanquin), 5% FBS ...
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bioRxiv - Immunology 2022Quote: Total B-cells were isolated from Buffy coat from healthy volunteers with written consent and ethics comity approval (Sanquin, the Netherlands) as described in Supplementary text ...
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bioRxiv - Immunology 2020Quote: ... IFN-γ or GM-CSF release in the cell culture supernatant was measured using ELISA according to the manufacturer’s protocol (Sanquin and BioLegend, respectively).
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bioRxiv - Immunology 2020Quote: ... Cytokine content of cell free supernatants was determined using standard sandwich ELISA according to the manufacturer’s instructions (IFN-γ and GM-CSF; Sanquin and Biolegend). Some target cells were pre-incubated for 2 days in the presence or absence of UGCG inhibitors.
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bioRxiv - Immunology 2022Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from Buffy coats from healthy donors after written informed consent (Sanquin Blood Bank, Nijmegen, the Netherlands). Samples were anonymized to safeguard donor privacy ...
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bioRxiv - Immunology 2020Quote: ... FcγRIII were immunoprecipitated from the neutrophil cell lysate using a mouse anti-CD16 monoclonal IgG2a antibody (Ref M9087, Clone CLB-FcR gran/1, 5D2, Sanquin, Amsterdam, The Netherlands). Prior to usage ...