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Citations for Sanquin :
1 - 41 of 41 citations for Corticosterone ELISA Kit 5 Whole Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... In a regular 96 ELISA flat bottom plate 15% normal human serum (Sanquin, Amsterdam, The Netherlands) was added to the liposome mixture (R1 from the kit ...
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bioRxiv - Immunology 2020Quote: ... plates were washed 5 times with PBS containing 0.02% Tween-20 (PBS; Sanquin Diagnostics) using an ELISA washer (Biotek ...
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bioRxiv - Cell Biology 2021Quote: ... were isolated from whole blood from healthy donors (Sanquin), stored O/N at RT ...
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bioRxiv - Immunology 2020Quote: ... CD4+CD45RA+ T cells were activated for 5-6 days with 1.5 µg/ml plate bound αCD3 (Sanquin) and 1 µg/ml soluble αCD28 (Sanquin ...
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bioRxiv - Cancer Biology 2022Quote: ... 2-3 wells containing 0.5-1 x106 live cells from the tissue digest were cultured for 10-13 days in 24 wells plates in 20/80 T-cell mixed media (Miltenyi) containing 5% human serum (HS) (Sanquin), 5% FBS ...
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bioRxiv - Immunology 2019Quote: ... 2-3 wells containing 0,5-1*106 live cells from the tissue digest were cultured for 10-13 days in 24 wells plates in 20/80 T-cell mixed media (Miltenyi) containing 5% human serum (HS) (Sanquin), 5% FBS ...
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bioRxiv - Immunology 2020Quote: ... and incubated with culture supernatants (diluted in high-performance ELISA buffer, Sanquin Reagents). Plates were again washed five times and incubated for one hour with horseradish peroxidase-conjugated mouse-anti-human-IgG (1 µg/ ml ...
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bioRxiv - Immunology 2020Quote: Peripheral blood mononuclear cells (PBMCs) were obtained from whole blood of healthy donors (from Sanquin Institute Amsterdam or from GSK Stevenage Blood Donation Unit ...
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bioRxiv - Neuroscience 2023Quote: Peripheral whole blood from 24 age-matched healthy controls (age >55 years) was obtained from Sanquin Rotterdam (NVT0585.00 Mantel ...
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bioRxiv - Cancer Biology 2023Quote: ... PE-conjugated streptavidin was diluted in high performance ELISA buffer (HPE) (Sanquin, Amsterdam, The Netherlands). The Biorad FlexMAP3D (Biorad laboratories ...
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bioRxiv - Cancer Biology 2023Quote: ... PE-conjugated streptavidin was diluted in high performance ELISA buffer (HPE) (Sanquin, Amsterdam, The Netherlands). The Biorad FlexMAP3D (Biorad laboratories ...
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bioRxiv - Immunology 2023Quote: Cytokines in cell-free supernatants were measured using enzyme-linked immunosorbent assay (ELISA) for IL-6 (Sanquin), TNF-α (Diaclone ...
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The transcription factor RUNX2 drives the generation of human NK cells and promotes tissue residencybioRxiv - Immunology 2022Quote: ... after which supernatant was collected and analysed with IFN-γ ELISA assay (PeliKine-Tool Set, Sanquin, Amsterdam, The Netherlands).
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bioRxiv - Immunology 2022Quote: ... 5% Human Serum (HS) (Sanquin), and 25 mM HEPES (Gibco ...
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bioRxiv - Bioengineering 2019Quote: ... and a subsequent incubation with phycoerythrin-conjugated streptavidin (10 min) which was diluted in high performance ELISA (HPE) buffer (Sanquin). Data acquisition was executed using a FLEXMAP 3D system controlled with xPONENT 4.1 software (Luminex ...
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bioRxiv - Bioengineering 2020Quote: ... the supernatants were consecutively incubated with antibody-conjugated MagPlex microspheres (1 h), biotinylated antibodies (1 h), and streptavidin-phycoerythrin (10 min, diluted in high performance ELISA (HPE) buffer (Sanquin)) ...
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The tumour microenvironment shapes dendritic cell plasticity in a human organotypic melanoma culturebioRxiv - Immunology 2019Quote: ... supplemented with 5% human serum (Sanquin), to sustain survival of immune cells in the reconstructed microenvironment (day 19) ...
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bioRxiv - Neuroscience 2021Quote: ... Plates were washed and incubated at room temperature for 30 minutes with horseradish peroxidase-conjugated streptavidin (strep-HRP; 1:10000 Streptavidin Poly HRP, Sanquin, Amsterdam, dilution in High Performance ELISA Buffer, HPE, Sanquin, Amsterdam). Plates were washed and 100 ul of substrate solution ...
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bioRxiv - Immunology 2022Quote: ... followed by 1 h incubation with biotinylated antibodies and 10 min incubation with phycoerythrin-conjugated streptavidin in high performance ELISA buffer (HPE, Sanquin, Hambug, Germany). Data acquisition was performed with FLEXMAP 3D equipment in combination with xPONENT software (version 4.3 ...
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bioRxiv - Immunology 2020Quote: ... IFN-γ or GM-CSF release in the cell culture supernatant was measured using ELISA according to the manufacturer’s protocol (Sanquin and BioLegend, respectively).
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bioRxiv - Immunology 2020Quote: ... Cytokine content of cell free supernatants was determined using standard sandwich ELISA according to the manufacturer’s instructions (IFN-γ and GM-CSF; Sanquin and Biolegend). Some target cells were pre-incubated for 2 days in the presence or absence of UGCG inhibitors.
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bioRxiv - Bioengineering 2022Quote: ... followed by 1 h incubation with biotinylated antibodies and 10 min incubation with phycoerythrin-conjugated streptavidin in high performance ELISA buffer (HPE, Sanquin, Hamburg, Germany). Data acquisition was performed with FLEXMAP 3D equipment in combination with xPONENT software (version 4.3 ...
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bioRxiv - Genomics 2020Quote: 5 male PBMC (Sanquin, Amsterdam, the Netherlands) and 3 placenta samples were a kind gift from the Department of Immunohematology and Blood Transfusion ...
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bioRxiv - Immunology 2022Quote: ... 5% human serum (Sanquin, Amsterdam, The Netherlands) and 100 IU/mL recombinant human IL-2 (Novartis ...
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bioRxiv - Immunology 2023Quote: ... 5% heat inactivated human serum (HS, Sanquin), 5% FCS and antibiotics supplemented with 120 U/mL IL-2 (Peprotech) ...
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bioRxiv - Immunology 2024Quote: 1-3×106 cells were cultured in 24-well plates at 37°C and 5% CO2 in 20/80 T-cell mixed media (Miltenyi) containing 5% human serum (HS) (Sanquin), 5% FBS ...
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bioRxiv - Immunology 2022Quote: ... and stimulated with plate-bound anti-CD3 (16A9, 1 µg/mL, Sanquin) and soluble anti-CD28 (15E8 ...
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bioRxiv - Immunology 2020Quote: ... T cell culture medium consisted of 5% human serum (Sanquin), 5% FCS ...
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bioRxiv - Microbiology 2023Quote: ... CHIKV virus stock (5 × 108 TCID50/mL) and full human blood (Sanquin) was 1:2 mixed to make the infectious blood meal with a final virus titer of 1.7× 108 TCID50/ml ...
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bioRxiv - Microbiology 2024Quote: ... Parasites were cultured in 5% human RBCs type O (Sanquin, the Netherlands) at 37 °C with 3 % O2 and 4 % CO2 ...
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bioRxiv - Microbiology 2021Quote: ... and 3-5% human blood type O red blood cells (RBCs) (Sanquin, the Netherlands) at 37°C in 3% O2 ...
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bioRxiv - Immunology 2019Quote: ... 24-well plates were pre-coated overnight with 0.5 μg/mL rat α-mouse IgG2a (clone MW1483, Sanquin) at 4 °C ...
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bioRxiv - Immunology 2020Quote: 10×106 cells/ml CD4+CD45RA+ T cells were cultured for 3 days in the presence of 1.5 µg/ml plate bound αCD3 (CLB-T3/4.E, 1XE Sanquin) and 1 µg/ml soluble αCD28 (CLB-CD28/1 ...
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bioRxiv - Immunology 2020Quote: ... Anti-RBD and anti-NP IgG antibodies were measured in EDTA plasma samples at 100-1200 fold dilutions using ELISA as previously described.11 Plates were coated with RBD or N protein and specific IgG antibodies were detected using anti-human IgG (MH16, Sanquin).
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bioRxiv - Immunology 2023Quote: ... were stimulated as described [6] in flat-bottom 96 well plates with a soluble anti-CD3 IgE subclass (T3/4.E, Sanquin, Amsterdam ...
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bioRxiv - Cancer Biology 2024Quote: Monocytes were seeded at a density of 1 x 10 cells per well in 96-well plates and incubated to mature for 6 days in RPMI-1640 medium containing 10% pooled human serum (Sanquin), 1% (v/v ...
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bioRxiv - Immunology 2022Quote: ... at a density of 0.8×106/mL in IMDM supplemented with 5% heat-inactivated human serum (Sanquin), 5% FBS and 100 IU/mL recombinant human (rh ...
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bioRxiv - Immunology 2019Quote: Human T cells were cultured in T-cell mixed media (Miltenyi) supplemented with 5% heat-inactivated human serum (Sanquin) and fetal bovine serum (FBS ...
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bioRxiv - Genomics 2021Quote: ... 10 ml of blood was obtained from 9 consenting anonymous healthy blood donors (HBDs; 5 females and 4 males) via the Dutch National blood bank (Sanquin) in CellSave tubes ...
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bioRxiv - Immunology 2020Quote: ... CD8+ T cell clones recognizing peptides derived from the endogenously expressed proteins USP11 and SSR1(7, 8) were expanded using a standard feeder mix in IMDM supplemented with 5% human serum (Sanquin) and 5% FCS(9).
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bioRxiv - Immunology 2021Quote: ... diluted in PBS for 10 min at RT and Fc-receptors were subsequently blocked using 5% human serum (HS; Sanquin Blood bank, Amsterdam, The Netherlands) for 45 min at RT ...