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Citations for Sanquin :
1 - 42 of 42 citations for 7 chloro 5 methylpyrazolo 1 5 A pyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... CD8+ T cell clones recognizing peptides derived from the endogenously expressed proteins USP11 and SSR1(7, 8) were expanded using a standard feeder mix in IMDM supplemented with 5% human serum (Sanquin) and 5% FCS(9).
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bioRxiv - Immunology 2022Quote: ... 5% Human Serum (HS) (Sanquin), and 25 mM HEPES (Gibco ...
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The tumour microenvironment shapes dendritic cell plasticity in a human organotypic melanoma culturebioRxiv - Immunology 2019Quote: ... supplemented with 5% human serum (Sanquin), to sustain survival of immune cells in the reconstructed microenvironment (day 19) ...
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bioRxiv - Genomics 2020Quote: 5 male PBMC (Sanquin, Amsterdam, the Netherlands) and 3 placenta samples were a kind gift from the Department of Immunohematology and Blood Transfusion ...
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bioRxiv - Immunology 2022Quote: ... 5% human serum (Sanquin, Amsterdam, The Netherlands) and 100 IU/mL recombinant human IL-2 (Novartis ...
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bioRxiv - Immunology 2023Quote: ... 5% heat inactivated human serum (HS, Sanquin), 5% FCS and antibiotics supplemented with 120 U/mL IL-2 (Peprotech) ...
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bioRxiv - Immunology 2024Quote: 1-3×106 cells were cultured in 24-well plates at 37°C and 5% CO2 in 20/80 T-cell mixed media (Miltenyi) containing 5% human serum (HS) (Sanquin), 5% FBS ...
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bioRxiv - Immunology 2020Quote: ... T cell culture medium consisted of 5% human serum (Sanquin), 5% FCS ...
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bioRxiv - Microbiology 2023Quote: ... CHIKV virus stock (5 × 108 TCID50/mL) and full human blood (Sanquin) was 1:2 mixed to make the infectious blood meal with a final virus titer of 1.7× 108 TCID50/ml ...
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bioRxiv - Microbiology 2024Quote: ... Parasites were cultured in 5% human RBCs type O (Sanquin, the Netherlands) at 37 °C with 3 % O2 and 4 % CO2 ...
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bioRxiv - Immunology 2020Quote: ... plates were washed 5 times with PBS containing 0.02% Tween-20 (PBS; Sanquin Diagnostics) using an ELISA washer (Biotek ...
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bioRxiv - Microbiology 2021Quote: ... and 3-5% human blood type O red blood cells (RBCs) (Sanquin, the Netherlands) at 37°C in 3% O2 ...
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bioRxiv - Immunology 2022Quote: ... at a density of 0.8×106/mL in IMDM supplemented with 5% heat-inactivated human serum (Sanquin), 5% FBS and 100 IU/mL recombinant human (rh ...
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bioRxiv - Immunology 2020Quote: ... CD4+CD45RA+ T cells were activated for 5-6 days with 1.5 µg/ml plate bound αCD3 (Sanquin) and 1 µg/ml soluble αCD28 (Sanquin ...
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bioRxiv - Immunology 2019Quote: Human T cells were cultured in T-cell mixed media (Miltenyi) supplemented with 5% heat-inactivated human serum (Sanquin) and fetal bovine serum (FBS ...
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bioRxiv - Genomics 2021Quote: ... 10 ml of blood was obtained from 9 consenting anonymous healthy blood donors (HBDs; 5 females and 4 males) via the Dutch National blood bank (Sanquin) in CellSave tubes ...
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bioRxiv - Cancer Biology 2022Quote: ... 2-3 wells containing 0.5-1 x106 live cells from the tissue digest were cultured for 10-13 days in 24 wells plates in 20/80 T-cell mixed media (Miltenyi) containing 5% human serum (HS) (Sanquin), 5% FBS ...
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bioRxiv - Immunology 2019Quote: ... 2-3 wells containing 0,5-1*106 live cells from the tissue digest were cultured for 10-13 days in 24 wells plates in 20/80 T-cell mixed media (Miltenyi) containing 5% human serum (HS) (Sanquin), 5% FBS ...
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bioRxiv - Immunology 2021Quote: ... diluted in PBS for 10 min at RT and Fc-receptors were subsequently blocked using 5% human serum (HS; Sanquin Blood bank, Amsterdam, The Netherlands) for 45 min at RT ...
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bioRxiv - Immunology 2020Quote: ... and 1 µg/ml soluble αCD28 (CLB-CD28/1, 15E8 Sanquin) in the presence or absence of milk EVs or EV depleted control ...
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bioRxiv - Immunology 2020Quote: ... and cultured with 70 ng/ml – 1 µg/ml soluble αCD28 (CLB-CD28/1, 15E8 from Sanquin), RPMI 1640 medium (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... CD63/Lamp3 (EM 1:20, #M1544, Sanquin), claudin 3 (IF 1:200 ...
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bioRxiv - Immunology 2020Quote: ... and 1 µg/ml soluble αCD28 (Sanquin) in medium ...
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bioRxiv - Bioengineering 2020Quote: ... the supernatants were consecutively incubated with antibody-conjugated MagPlex microspheres (1 h), biotinylated antibodies (1 h), and streptavidin-phycoerythrin (10 min, diluted in high performance ELISA (HPE) buffer (Sanquin)) ...
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bioRxiv - Immunology 2020Quote: ... and IgG-DyLight 650 (clone MH16-1, Sanquin Reagents ...
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bioRxiv - Immunology 2020Quote: ... were coated overnight with anti-IgG1 or anti-IgG4 (2 µg/ml clones MH161-1, MH161-1, MH164-4 respectively, Sanquin Reagents) in PBS ...
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bioRxiv - Immunology 2020Quote: ... Plates were again washed five times and incubated for one hour with horseradish peroxidase-conjugated mouse-anti-human-IgG (1 µg/ ml, clone MH16-1, Sanquin Reagents). After a final five-times wash ...
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bioRxiv - Cancer Biology 2021Quote: ... Granzyme B-PE (1:200, clone GB11, Sanquin Amsterdam). To detect cytokine production ...
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bioRxiv - Bioengineering 2022Quote: ... human plasma-derived holotransferrin (1 mg/mL; Sanquin; Netherlands), and heparin (5 U/mL ...
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bioRxiv - Immunology 2022Quote: ... and CD28 (clone CLB-CD28/1, Sanquin, 0.2 μg/ml) in solution ...
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bioRxiv - Immunology 2022Quote: ... and soluble anti-CD28 (15E8, 1 µg/mL, Sanquin (10)) ...
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bioRxiv - Immunology 2022Quote: ... and stimulated with plate-bound anti-CD3 (16A9, 1 µg/mL, Sanquin) and soluble anti-CD28 (15E8 ...
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bioRxiv - Immunology 2024Quote: ... Anti-CD103-BV711 (Ber-ACT8) and anti-Hobit-Alexa647 (Sanquin-Hobit/1) were purchased from BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... and anti-IgG (clone G18-145, BD Bioscience; clone MH16-1, Sanquin Reagents). Samples were measured on LSRII and analyzed using Flowjo software (Treestar).
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bioRxiv - Cell Biology 2020Quote: ... Sections were blocked for 30 min with 1% bovine serum albumin (BSA; Sanquin, The Netherlands) in tris-buffered saline (TBS) ...
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bioRxiv - Immunology 2019Quote: ... Platelets were re-pelleted and resuspended in 1 mL SSP+ (Storage solution for platelets, Sanquin, Amsterdam, the Netherlands). Platelet count and platelet activation were determined by flow cytometry (FACS Calibur ...
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bioRxiv - Immunology 2021Quote: ... biotinylated recombinant antibodies that were able to bind the antigen were detected with 1/10,000 poly-HRP-conjugated streptavidin (Sanquin) diluted in PTA buffer (100 μL/well ...
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bioRxiv - Neuroscience 2021Quote: ... Plates were washed and incubated at room temperature for 30 minutes with horseradish peroxidase-conjugated streptavidin (strep-HRP; 1:10000 Streptavidin Poly HRP, Sanquin, Amsterdam ...
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bioRxiv - Immunology 2023Quote: ... Purified CD4+ T cells were seeded at 0.5*10^6 cells/ml in plates coated with 0.5 μg/ml anti-CD3 (CLB-T3/4.E, 1XE) and 70 ng/ml soluble anti-CD28 (CLB-CD28/1, 15E8; both from Sanquin). Cells were cultured in a total volume of 1 ml consisting of medium only ...
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bioRxiv - Neuroscience 2021Quote: ... Plates were washed and incubated at room temperature for 30 minutes with horseradish peroxidase-conjugated streptavidin (strep-HRP; 1:10000 Streptavidin Poly HRP, Sanquin, Amsterdam, dilution in High Performance ELISA Buffer, HPE, Sanquin, Amsterdam). Plates were washed and 100 ul of substrate solution ...
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bioRxiv - Immunology 2022Quote: ... plates were washed five times with PBS-T and incubated with 1 µg/ml anti-human IgG-horseradish-peroxidase (HRP) (clone: MH16.1, Sanquin, Amsterdam, the Netherlands). After washing ...
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bioRxiv - Immunology 2020Quote: ... FcγRIII were immunoprecipitated from the neutrophil cell lysate using a mouse anti-CD16 monoclonal IgG2a antibody (Ref M9087, Clone CLB-FcR gran/1, 5D2, Sanquin, Amsterdam, The Netherlands). Prior to usage ...