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Citations for Sanquin :
1 - 27 of 27 citations for 7 11 Dimethyldodeca 4 6 10 trien 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... were stimulated as described [6] in flat-bottom 96 well plates with a soluble anti-CD3 IgE subclass (T3/4.E, Sanquin, Amsterdam ...
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bioRxiv - Immunology 2020Quote: 10×106 cells/ml CD4+CD45RA+ T cells were cultured for 3 days in the presence of 1.5 µg/ml plate bound αCD3 (CLB-T3/4.E, 1XE Sanquin) and 1 µg/ml soluble αCD28 (CLB-CD28/1 ...
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bioRxiv - Cancer Biology 2024Quote: Monocytes were seeded at a density of 1 x 10 cells per well in 96-well plates and incubated to mature for 6 days in RPMI-1640 medium containing 10% pooled human serum (Sanquin), 1% (v/v ...
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bioRxiv - Immunology 2024Quote: ... 6% human serum (Sanquin, Amsterdam, the Netherlands), 200 mM L-glutamine ...
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bioRxiv - Immunology 2020Quote: ... CD8+ T cell clones recognizing peptides derived from the endogenously expressed proteins USP11 and SSR1(7, 8) were expanded using a standard feeder mix in IMDM supplemented with 5% human serum (Sanquin) and 5% FCS(9).
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bioRxiv - Immunology 2020Quote: ... Plates were again washed five times and incubated for one hour with horseradish peroxidase-conjugated mouse-anti-human-IgG (1 µg/ ml, clone MH16-1, Sanquin Reagents). After a final five-times wash ...
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bioRxiv - Immunology 2023Quote: Cytokines in cell-free supernatants were measured using enzyme-linked immunosorbent assay (ELISA) for IL-6 (Sanquin), TNF-α (Diaclone ...
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bioRxiv - Immunology 2020Quote: ... CD4+CD45RA+ T cells were activated for 5-6 days with 1.5 µg/ml plate bound αCD3 (Sanquin) and 1 µg/ml soluble αCD28 (Sanquin ...
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bioRxiv - Microbiology 2021Quote: ... and 3-5% human blood type O red blood cells (RBCs) (Sanquin, the Netherlands) at 37°C in 3% O2 ...
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bioRxiv - Immunology 2022Quote: ... and soluble anti-CD28 (15E8, 1 µg/mL, Sanquin (10)) ...
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bioRxiv - Immunology 2020Quote: ... coated with 1.5 µg/ml αCD3 (CLB-T3/4.E, 1XE from Sanquin, Amsterdam, The Netherlands) and cultured in RPMI 1640 medium (Gibco ...
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bioRxiv - Immunology 2019Quote: ... were pre-coated overnight at 4°C with 4μg/mL rat α-mouse IgG2a (MW1483, Sanquin) in PBS ...
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bioRxiv - Immunology 2020Quote: ... coated with 0.5 – 1.5 µg/ml αCD3 (CLB-T3/4.E, 1XE from Sanquin, Amsterdam, The Netherlands) and cultured with 70 ng/ml – 1 µg/ml soluble αCD28 (CLB-CD28/1 ...
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bioRxiv - Immunology 2022Quote: ... Cells were expanded using agonistic mAbs against CD3 (clone CLB-T3/4.E, IgE isotype, Sanquin, 0.1 μg/ml) and CD28 (clone CLB-CD28/1 ...
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bioRxiv - Genomics 2021Quote: ... 10 ml of blood was obtained from 9 consenting anonymous healthy blood donors (HBDs; 5 females and 4 males) via the Dutch National blood bank (Sanquin) in CellSave tubes ...
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bioRxiv - Immunology 2023Quote: ... Purified CD4+ T cells were seeded at 0.5*10^6 cells/ml in plates coated with 0.5 μg/ml anti-CD3 (CLB-T3/4.E, 1XE) and 70 ng/ml soluble anti-CD28 (CLB-CD28/1, 15E8; both from Sanquin). Cells were cultured in a total volume of 1 ml consisting of medium only ...
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bioRxiv - Immunology 2023Quote: ... Treg cells (105 cells/well) were added and the medium was supplemented with soluble anti-CD3ε [25] (T3/4.E, Sanquin). T3/4.E is able to stimulate Tresp cells directly in soluble form [25] ...
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bioRxiv - Immunology 2020Quote: ... were coated overnight with anti-IgG1 or anti-IgG4 (2 µg/ml clones MH161-1, MH161-1, MH164-4 respectively, Sanquin Reagents) in PBS ...
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bioRxiv - Neuroscience 2021Quote: Blood samples of 10 healthy controls were obtained from the Dutch blood bank (Sanquin, https://www.sanquin.nl/en) (Supplementary Table 1) ...
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bioRxiv - Cancer Biology 2021Quote: ... 2011) where TEGs were cultured in ‘TEG culture medium’ (RPMI-Glutamax supplemented with 2,5 – 10 % human serum (Sanquin), 1% pen/strep and 0.5M beta-2-mercaptoethanol ...
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bioRxiv - Bioengineering 2019Quote: ... and a subsequent incubation with phycoerythrin-conjugated streptavidin (10 min) which was diluted in high performance ELISA (HPE) buffer (Sanquin). Data acquisition was executed using a FLEXMAP 3D system controlled with xPONENT 4.1 software (Luminex ...
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bioRxiv - Bioengineering 2020Quote: ... the supernatants were consecutively incubated with antibody-conjugated MagPlex microspheres (1 h), biotinylated antibodies (1 h), and streptavidin-phycoerythrin (10 min, diluted in high performance ELISA (HPE) buffer (Sanquin)) ...
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bioRxiv - Immunology 2021Quote: Monocytes were seeded at a density of 100 000 cells per well in 96-well plates and were maturated for six days in Roswel Park Memorial Institute-1640 (RPMI-1640) medium containing 10% pooled human serum (Sanquin), 1% (v/v ...
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bioRxiv - Immunology 2019Quote: ... peripheral blood and uterine Tregs and Tconv were washed in PBS and resuspended in basic medium with 10% human AB serum (Sanquin). Previously isolated and frozen healthy donor (HC ...
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bioRxiv - Immunology 2022Quote: ... followed by 1 h incubation with biotinylated antibodies and 10 min incubation with phycoerythrin-conjugated streptavidin in high performance ELISA buffer (HPE, Sanquin, Hambug, Germany). Data acquisition was performed with FLEXMAP 3D equipment in combination with xPONENT software (version 4.3 ...
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bioRxiv - Bioengineering 2022Quote: ... followed by 1 h incubation with biotinylated antibodies and 10 min incubation with phycoerythrin-conjugated streptavidin in high performance ELISA buffer (HPE, Sanquin, Hamburg, Germany). Data acquisition was performed with FLEXMAP 3D equipment in combination with xPONENT software (version 4.3 ...
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bioRxiv - Immunology 2021Quote: ... diluted in PBS for 10 min at RT and Fc-receptors were subsequently blocked using 5% human serum (HS; Sanquin Blood bank, Amsterdam, The Netherlands) for 45 min at RT ...