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Citations for Vector Labs :
501 - 550 of 659 citations for 5 Methylsulfamoylmethyl 1H indole 3 carboxylic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... The sections were then washed 3 times and treated with avidin-biotin complex solution (VECTASTAIN® Elite ABC-HRP Kit, Vector Lab, #PK-6100) for 90 min at RT ...
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bioRxiv - Neuroscience 2021Quote: ... Following one (cell lines) or 3 days expression (primary hippocampal cultures) cells were fixed using paraformaldehyde and coverslips mounted using Vectashield (Vector Laboratories, Burlingame, CA, USA) with or without DAPI (cell lines) ...
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bioRxiv - Physiology 2022Quote: ... cells were washed 3 times with PBS on ice and bound biotinyl tyramide was visualized with fluorescent streptavidin (Vector Laboratories, Inc., Burlingame, CA, USA) at 1:1000 in PBS for 30 minutes at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... then reacted for visualization of peroxidase with nickel-cobalt intensified diaminobenzidine for 3 - 8 minutes (DAB Peroxidase substrate kit SK-4100, Vector Laboratories, Burlingame, CA, USA). Brain slices were washed between each incubation step using TBS (3 × 10 minutes ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and incubated for 2-3 hours with biotinylated anti-rabbit secondary antibody (1:450 for DeltaFosB and 1:600 for c-Fos, Vector Laboratories, Burlingame, CA, USA). Finally ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed in PBS 3 more times (10 min, room temperature) and mounted to microscope slides using VECTASHIELD Antifade Mounting Medium with DAPI (Vector Laboratories, cat. # H-1200-10). Sections were imaged on a Nikon A1R confocal microscope at the Tufts University Center for Neuroscience Research Microscopy Core ...
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bioRxiv - Neuroscience 2024Quote: ... Area X (n = 4) and (ii) HVC (n = 3; [14]. The sections were rinsed in PBS, subjected to antigen retrieval (Vector Laboratories, H-3300, pH 6) and treated with 2N HCl ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were washed with PBS (3 x 10 min) and incubated with biotinylated goat anti-rabbit secondary antibody (1:600, Vector Laboratories, Cat# BA-1000, RRID:AB_2313606), diluted in 1% NGS in PBS with Triton X-100 ...
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bioRxiv - Neuroscience 2024Quote: ... 3 x 10min PBS washes were followed by mounting in VECTASHIELD® HardSet™ mounting medium with DAPI (Vector Labs, UK, Cat# H-1500-10).
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bioRxiv - Cell Biology 2020Quote: ... washed three times for 5 min in PBST and mounted in Vectashield mounting medium with DAPI (H-1200; Vector Laboratories). Primary antibodies used for immunofluorescence were ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were washed three times for 5 min in PBT and mounted in VectaShield medium (Vector Laboratories, cat#H-1000). Images were acquired on a Zeiss LSM 710 confocal microscope using a 25X immersion objective (Zeiss ...
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bioRxiv - Developmental Biology 2021Quote: ... sections were blocked with 5% donkey serum (and Mouse-on-Mouse block as necessary from Vector Laboratories, Inc., Burlingame, CA) and incubated overnight at 4°C with an antibody against either Perlecan (1:50 ...
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bioRxiv - Immunology 2021Quote: ... The eOD-60mer was affinity purified by incubating with Galanthus Nivalis Lectin-conjugated agarose beads (AL-1243-5, Vector Laboratories) overnight under gentle agitation at 4°C and eluted with lectin elution buffer containing 1 M Methyl a-D-mannopyranoside (M6882 ...
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bioRxiv - Physiology 2020Quote: ... the sections were stained using the avidin-biotinylated-peroxidase ABC kit followed by a 5-min treatment with 3,3’-diaminobenzidine (DAB, SK-4100, Vector Laboratories). Stained sections were observed and imaged on a Nikon E-1000 microscope (Japan ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed in 1x PBS three times for 5 min and mounted with Vectashield antifade mounting medium (Vector Laboratories).
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bioRxiv - Neuroscience 2020Quote: ... Slices were washed three times in PBT for 5 minutes then incubated in biotinylated anti-rabbit IgG (Vectastain, Vector Laboratories) at a concentration of 1:200 in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... brain sections were blocked for 1 h at room temperature in 5% normal goat serum (NGS; Vector Laboratories, Burlingame, CA) diluted in 0.01 M PBS/0.1% Triton-X permeabilization buffer ...
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bioRxiv - Immunology 2022Quote: ... washed in PBS+0.1% Tween 20 for 5 min three times and mounted with mounting media containing DAPI (Vector Laboratories). Fluorescent images were captured using BZ-X810 Inverted Microscope (Keyence).
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bioRxiv - Immunology 2022Quote: ... The cells were washed and stained for 30 min at 4°C with 5 μg/ml FITC- WGA (Vector Laboratories). After the wash ...
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bioRxiv - Molecular Biology 2020Quote: ... brain sections were blocked for 1 h at room temperature in 5% normal goat serum (NGS; Vector Laboratories, Burlingame, CA) diluted in 0.01 M PBS/0.1% Triton-X permeabilization buffer ...
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Noradrenergic alpha-2A receptor activation suppresses courtship vocalization in male Japanese quail.bioRxiv - Animal Behavior and Cognition 2021Quote: ... The sections were then washed with PBS three times for 5 min and incubated with avidin-biotin complex reagent at a dilution based on the manufacturer’s recommendations (Vector Laboratories) for 30 min at RT ...
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bioRxiv - Physiology 2020Quote: ... incubated in 4’6’-diamidino-2-phenylindole (DAPI) (1:1000) in TBS for 5 minutes then mounted in Vectashield (Vector Laboratories). Fluorescent images were captured using a Nikon A1R-s confocal laser scanning microscope at the BCM Integrated Microscopy Core and processed with the Nikon Perfect Focus System (Nikon Corporation ...
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bioRxiv - Neuroscience 2020Quote: ... brain sections were blocked for 1 h at room temperature in 5% normal goat serum (NGS; Vector Laboratories, Burlingame, CA) diluted in 0.01 M PBS/0.1% Triton-X permeabilization buffer ...
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bioRxiv - Neuroscience 2021Quote: ... brain sections were blocked for 1 h at room temperature in 5% normal goat serum (NGS; Vector Laboratories, Burlingame, CA) diluted in 0.01 M PBS/0.1% Triton-X permeabilization buffer ...
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bioRxiv - Genetics 2021Quote: ... the sections were washed 3 times in PBS for 5 minutes each and mounted with Vectashield mounting medium containing 4,6-diamidino-2-phenylindole (DAPI, Vector Laboratories) and sealed with nail polish ...
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bioRxiv - Immunology 2021Quote: ... Tissue sections were fixed with ice cold acetone for 5 min and blocked with Avidin/Biotin Blocking Kit (Vector laboratories) and Protein block (Protein block (Dako ...
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bioRxiv - Neuroscience 2022Quote: ... endogenous peroxidase activity was blocked with 0.3 % H2O2 for 10 min and sections were blocked with 5 % normal goat serum (Vector Labs) in PBS for 1 h at RT ...
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bioRxiv - Physiology 2022Quote: ... EDL and TA sections were stained with 1:1000 fluorescein wheat germ agglutinin (WGA; 5 μg/mL; Vector Laboratories Ltd.) for 10 min ...
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bioRxiv - Neuroscience 2024Quote: ... incubated in secondary antibody in 5% NGS for at least 2 overnights and mounted using Vectashield (Vector Laboratories H-1000). After each step ...
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bioRxiv - Neuroscience 2024Quote: ... After an hour of cool-down slides were washed with PBS and incubated with blocking buffer (1% BSA, 5% FBS, 0.01 % Triton-X 100 in 1x PBS) followed by Avidin/Biotin blocking (Vector laboratories). Slides were incubated overnight at 4°C with primary antibodies (see Supplementary Table 4 ...
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bioRxiv - Neuroscience 2024Quote: ... brain sections were blocked for 1 h at room temperature in 5% normal goat serum (NGS; Vector Laboratories, Burlingame, CA) diluted in 0.01 M PBS/0.1% Triton-X permeabilization buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were washed in TBS and incubated for 5 minutes in 3,3′-Diaminobenzidine (DAB) (SK-4105, Vector laboratories, CA, USA). Slides were rinsed in water ...
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bioRxiv - Plant Biology 2023Quote: ... slides were washed 3 x 5 min in 1 x PBS and mounted in Vectashield mounting medium with 1.5 μg/mL DAPI (Vector Laboratories). Antibodies and dilutions used in this study are reported in Supplementary Table 3 ...
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bioRxiv - Genetics 2023Quote: ... cells were washed with PBS three times for 5 minutes and mounted with Vectorshield mounting medium with DAPI (Vector Laboratories). Images were captured by the Leica fluorescence microscope ...
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bioRxiv - Immunology 2023Quote: ... and then run slowly over a column with agarose-bound Galanthus nivalis lectin (Vector Laboratories cat no. AL-1243-5) at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... Excess DAPI was washed off with 0.1% Triton X-100/PBS for 5 min and samples mounted with VECTASHIELD (Vector Labs). Images were acquired using Leica TCS SP8 Confocal Microscope ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were blocked with 5% normal goat serum followed by an Avidin/Biotin blocking kit (Vector Labs, Burlingame, CA, USA) and then incubated overnight at room temperature with the primary antibody ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were incubated with 2 µg/mL biotinylated lectins from Vector Labs (VVA B-1235-2, PNA B-1075-5) in conditions as previously described ...
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bioRxiv - Molecular Biology 2024Quote: ... according to the manufacturer’s protocol before samples were stained with DAPI (1 µg/ml, 5 min) and mounted in Vectashield (Vector Laboratories). Images were collected with a Leica SP5 II confocal microscope using LAS-AF software (v2.7.3.9723) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Sections were washed in 1XPBS for 5 minutes and incubated for 20 minutes with prediluted normal blocking serum (Vector Labs). The sections were incubated for 30 minutes with 60μl Yap/Taz primary antibody (8418 ...
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bioRxiv - Neuroscience 2024Quote: ... A final wash in PBS (for 5 minutes x2) was completed and then 3,3-diaminobenzidine tetrahydrochloride (DAB) (Vector Laboratories, UK) was applied to help visualize the antibody (left for 3 minutes) ...
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bioRxiv - Immunology 2024Quote: ... diluted 1:1000 in 5% nonfat dry milk/TBS-T or goat anti-rabbit IgG-HRP (Vector Laboratories #PI-1000) diluted 1:20,000 in 5% nonfat dry milk/TBS-T – for 1 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed three times in PBS for 5 min and mounted using Vectashield® medium (Vector Laboratories, Burlingame, CA) and coverslips ...
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bioRxiv - Cancer Biology 2024Quote: ... This was followed by incubation for 5 minutes with a quencher (Vector TrueView Autofluorescence Quenching Kit #SP-8400, Vector Labs) to decrease autofluorescence ...
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bioRxiv - Cell Biology 2020Quote: ... 0.5% TritonX-100 in PBS) for 30 min at RT followed by another blocking using M.O.M kit (Vector Laboratories BMK-2202). Primary antibodies (mouse anti-MYH3 ...
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bioRxiv - Cell Biology 2020Quote: ... The organoids were centrifuged at 500 x g for 5 min and resuspended in 30 μL Vectashield (Vector Labs; H-1000) using a cut pipet tip and pipetted onto a coverslide ...
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bioRxiv - Genetics 2021Quote: ... slides were washed 3 times in PBS-T for 5 min each and incubated with the biotinylated horse anti-mouse IgG antibody (AK-5002, Vector laboratories) for 30 min ...
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bioRxiv - Biochemistry 2021Quote: ... and treated with or without PNGase F was assayed by lectin blotting using Concanavalin A (Con A) (Cat. B-1005-5, Vector Laboratories). A total of 10 μg protein each were electrophoresed in a 12% SDS-PAGE gel and transferred onto PVDF membranes at a constant of 300 mA for 2 h ...
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bioRxiv - Genetics 2021Quote: ... then blocked with 5% (v/v) horse serum (ImmPRESS HRP Anti-Rabbit IgG [Peroxidase] Polymer Detection Kit; Vector labs; MP-7401). Sections were incubated with rabbit anti-TCFL5 (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... DNMT3A and DNMT3B was visualized in cross-section (5 µm) of paraffin-embedded tissues using specific primary antibodies and a Vectastain Elite ABC Kit (Vector Laboratories) or AlexaFluor 488-conjugated secondary antibodies (Invitrogen) ...