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Citations for Vector Labs :
451 - 500 of 659 citations for 5 Methylsulfamoylmethyl 1H indole 3 carboxylic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... No1.5H with a thickness of 170 μm ± 5 μm) were used in cell culture and Vectashield (Vector Laboratories) was used as mounting reagent ...
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bioRxiv - Immunology 2020Quote: ... Sections were rehydrated in PBS for 5 to 10 minutes and blocked using biotin blocking kit (Vector Laboratories) followed by incubation with 1%(v/v ...
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bioRxiv - Cell Biology 2022Quote: ... tissues were washed again in PBS (20 min x 5) and incubated with the ABC complex (Vector Labs). The antigen-antibody reaction was visualized using 3,3′-Diaminobenzidine (DAB ...
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bioRxiv - Neuroscience 2024Quote: ... Then sections were incubated in a blocking solution containing 5% normal goat serum (NGS; S-1000, Vector Laboratories), 1% BSA and 0.25% TX in TB for 60 min at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... Slides were then washed 4 × 5 min in PBS and subsequently mounted with Vectashield mounting medium (Vector Laboratories) with DAPI ...
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bioRxiv - Neuroscience 2023Quote: ... tissue sections were first blocked in either 5% normal goat serum (Cat # S-1000; Vector Laboratories, Burlingame, CA) or 5% normal donkey serum (Cat # 017-000-121 ...
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bioRxiv - Neuroscience 2023Quote: ... (pH 7.6) for 5 minutes before incubating with the secondary antibody (HRP-conjugated ImmPRESS Polymer Reagent, Vector Labs) for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Blocking for 1 h with 5% (vol/vol) normal goat or rabbit serum (NGS or NRS) (Vector Laboratories, #S-1000 ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed 4-5 times in 1X PBS and transferred into ABC Kit solution (PK4000, Vector Laboratories) containing 10µl of solution A and 10µl of solution B for 1ml of 1X PBS ...
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bioRxiv - Cell Biology 2024Quote: ... was applied for 5 minutes before final rinse in PBS and mounting with Vectashield (Vector Laboratories, Burlingame, California).
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bioRxiv - Cell Biology 2020Quote: ... The coverslips with cells were mounted on 3 cm glass bottom cell culture plates using Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA). Random fields on the coverslips were photographed using an inverted Olympus IX81 microscope (Olympus Europe Holding Gmbh ...
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bioRxiv - Neuroscience 2020Quote: ... Brain sections that contained the ventrolateral preoptic nucleus were collected and blocked in PBS containing 0.25% Triton X-100 and 3% normal donkey serum (Vector Laboratories, Burlingame, CA, USA) for 60 min at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were then washed with 0.1% PBST for 3 × 10 minutes and carefully cut into 4 quadrants and mounted on slides with DAPI Vectashield (Vector Laboratories, H-1200). For cryosections ...
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bioRxiv - Cell Biology 2021Quote: ... pelleted (400 g, 6 minutes) and resuspended in 2 to 3 drops (∼75 μL) of Vectashield Antifade Mounting Medium (Vector Laboratories, H-1000). This suspension was then mixed thoroughly by pipetting to separate clumped cells ...
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bioRxiv - Cancer Biology 2022Quote: ... antibody (1 µg/mL, 3 h; Santa Cruz, #SC-816; secondary: biotinylated goat anti-rabbit IgG, 1:200, 16 minutes; Vector labs, #PK6101), or an anti-c-MYC antibody (1:100 ...
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bioRxiv - Neuroscience 2020Quote: Sections were again washed 3×15 min with PBS and incubated in avidin-biotin complex solution (Vectastain Elite ABC kit, Vector Laboratories, Burlingame, CA) (2-hours ...
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bioRxiv - Pathology 2020Quote: ... cryoslides were washed 3 times in PBS and then slides were fluoromounted using Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA, USA).
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bioRxiv - Cell Biology 2020Quote: ... The signal was revealed using Vectastain Elite ABC-HRP kit and AEC Peroxidase (HRP) substrate 3-amino-9ethylcarbazole (VECTOR AEC Peroxidase Subtract Kit SK-4200, Vector Laboratories, Burlingame, CA). Samples were mounted using ProLong Gold antifade reagent (Life Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... The slides were then rinsed in diH2O and counterstained in a 1:3 diluted solution of Nuclear fast red (Vector labs H-3403). Counterstaining was done for no longer than 3 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then washed 3 times with PBS and mounted on slides with VECTASHIELD® Vibrance™ Antifade Mounting Medium with DAPI (Vector laboratories).
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bioRxiv - Neuroscience 2022Quote: The sections were rinsed in PB and to block non-specific antibody binding they were preincubated for 1 h at room temperature in a stock solution containing 3% normal serum of the species in which the secondary antibodies were raised (Vector Laboratories, Burlingame, CA) in PB with Triton X-100 (0.25%) ...
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bioRxiv - Cell Biology 2024Quote: ... Peroxidase was quenched by incubating them in 3% hydrogen peroxide (H2O2) for 10 min and they were then blocked with 1% normal horse serum (Vector Laboratories, SP-2001) at room temperature for 15 min ...
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bioRxiv - Molecular Biology 2023Quote: ... In the end slides were washed 3 times with 0.1%Tween in PBS and mounted with VECTASHIELD Antifade Mounting Medium with DAPI (Vector Laboratories, H-1200-10). The images were acquired using Zeiss LSM800 confocal microscope and analyses using Fiji software 86 and visualized and deposited using OMERO 87 (project ID:12920).
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bioRxiv - Bioengineering 2022Quote: ... Retinas were then washed 3 times in PBS at RT and incubated with polyclonal rabbit LM opsin and S opsin antibody (1/300; Vector Laboratories, Peterborough, UK) for 48 hours at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... the slides were incubated with secondary antibodies for 1 hour and washed again 3 times with PBS and mounted with Vectaschield containing DAPI (Vector Laboratories, H-1500).
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bioRxiv - Neuroscience 2023Quote: ... 0.3% Triton X-100 (24 hours, 37°C) followed by peroxidase-conjugated secondary antibody (1:200 biotinylated anti-rabbit Vector Labs #BA-1000) and incubated in Elite ABC solution ...
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bioRxiv - Neuroscience 2024Quote: ... for 30 seconds to block endogenous fluorescence signal before washing in PBS (3 x 2 mins) and cover slipped and mounting with Vectashield antifade mounting medium with DAPI (H1900, Vector Laboratories, Peterborough, UK).
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bioRxiv - Neuroscience 2024Quote: ... slides were washed with PBS and incubated for at least an hour with blocking buffer containing 3% normal goat serum (NGS; Vector Laboratories, Burlingame, CA) and 0.4% Triton X-100 (Sigma Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... and after 3 more washes with PBS the coverslips were mounted with Vectashield solution (mounting medium with DAPI, Vector Laboratories Inc., Burlingame, CA). Evaluation was done using a Nikon 80i or Bio-rad confocal microscope ...
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bioRxiv - Molecular Biology 2021Quote: ... each for 5 min using a 42°C hot plate) before being mounted with VECTASHIELD with DAPI (Vector Labs) onto Superfrost Plus microscopy slides (VWR) ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were blocked with DPBS containing 5% (w/v) normal goat serum (Vector Laboratories, Burlingame, CA, United States), 5% (w/v ...
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bioRxiv - Cell Biology 2021Quote: ... sectioned (5 um) and processed for immunohistochemical analysis post heat-mediated antigen retrieval at pH 6.0 (Vector Labs, USA) except sections stained with anti-SOD1 antisera that were subject to antigen retrieval at pH 9.0 (Vector Labs ...
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bioRxiv - Biophysics 2020Quote: ... 1.6 kb) or control nontelomeric DNA (noTel, 4.1 kb) using the 5’ EndTag Labeling DNA/RNA Kit (Vector Laboratories). The biotinylation of the pT270 and noTel fragments was verified using AFM imaging of DNA samples in the presence of streptavidin-coated quantum dots (strep-QDs ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were incubated overnight with Dolichos biflorus lectin conjugated to Tetramethylrhodamine-5-(and 6)-isothiocyanate (DBA-TRITC, Vector Lab) at 10 μg/mL diluted in blocking buffer at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Peanut Agglutinin conjugated with rhodamine was used at 1:1000 to identify cones (Vector Laboratories, catalog # RL-1072-5). Secondary antibodies ...
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bioRxiv - Immunology 2020Quote: ... coverslips were washed 5 times with 1×PBS and mounted with VECTASHIELD antifade mounting medium (Vector Laboratories, CA, USA). Nuclear intensity of at least 15 infected cells was measured for each experiment and coverslip.
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bioRxiv - Neuroscience 2021Quote: ... and 1000 mM) or 500 mM KCl in 0.1 Trizma buffer with 5 - 10% neurobiotin (Vector Labs, Birmingame, USA). They were then beveled from an initial resistance of 40 - 150 MΩ to 30 - 40 MΩ using a rotary beveller (Sutter BV-10) ...
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bioRxiv - Microbiology 2021Quote: ... Cell nuclei were labeled with Hoechst 1:10,000 for 5 minutes and coverslips were mounted using Vectashield (Vector Laboratories). Slides were imaged using a Leica DM6000 Widefield microscope.
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bioRxiv - Cell Biology 2021Quote: ... washed three times with PBS at RT for 5 min and finally mounted with VECTASHIELD mounting medium (Vector Laboratories). Primary antibodies were rabbit antibodies against DDX6 (Bethyl ...
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bioRxiv - Molecular Biology 2024Quote: ... and washed two times for 5 min in MQ before mounting with Vectashield mounting media (Vector Laboratories, #H-1000). Lipid content was quantified using Qupath version 0.2.2 ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were washed three times with PBS and then blocked with normal horse serum (NHS; 5%; Vector Laboratories) for 1h at RT or overnight at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... 5 % of Potter-dissociated whole brain homogenate was labelled with rhodamine-conjugated Dolichos Biflorus Agglutinin (Vector Laboratories RL-1032). Cysts were counted using an inverted fluorescence microscope with a 20X objective ...
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bioRxiv - Cell Biology 2023Quote: ... After washing for 5 min three times the samples were mounted with antifade mounting medium (Vector Laboratories, H-1700). The samples were imaged on Leica TCS SP8 STED 3X CW 3D microscope using HC PL APO 93x/1.30 motCORR STED WHITE (glycerol ...
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bioRxiv - Cell Biology 2022Quote: ... 5 µg/ml) and rhodamine-conjugated Ulex Europaeus Agglutinin I (UEA I) (RL-1062, Vector Laboratories, 10 µg/ml) was applied on the slides ...
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bioRxiv - Genomics 2023Quote: ... each for 5 min using a 42°C hot plate) before being mounted with VECTASHIELD plus DAPI (Vector Labs) onto Superfrost Plus microscopy slides (VWR) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The sections were then washed for 5 minutes in PBS and stained with DAB Peroxidase Substrate Solution (Vector Labs), for 3 minutes under the microscope ...
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bioRxiv - Genetics 2024Quote: ... The chromosomes were counterstained with 5 μg/mL of 4,6-diamidino-2-phenylindole (DAPI) in Vectashield (Vector Laboratories, USA) then observed under a BX43 phase-contrast microscope or a BX-53 fluorescence microscope (both from Olympus ...
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bioRxiv - Genetics 2024Quote: ... 6-diamidine-2-phenylindole dihydrochloride in 2XSSC) for 5 min and then mounted in Vectashield H-1000 (Vector Laboratories). Samples were imaged using a fluorescent Nikon microscope equipped with a cooled CoolSnap CCD camera (Photometrics) ...
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bioRxiv - Plant Biology 2024Quote: ... 4’,6-diamidino-2-phenylindole (DAPI) was diluted to 5 μg/mL in Vectashield antifade mounting media (Vector Laboratories).
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bioRxiv - Neuroscience 2022Quote: ... This was followed by an incubation with biotinylated goat anti-rabbit secondary antibody (1:200 in 3% NGS + 0.1% Triton X-100; Vector Labs, Burlingame, CA; Lot# 140893). Next ...