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Citations for Mabtech :
1 - 49 of 49 citations for 9 10 12 13 tetrahydroxyoctadecanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Streptavidin–HRP (Mabtech, Sweden, Cat: 3310-9) was diluted 1:1000 in PBS containing 1% BSA and added 100μl/well ...
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bioRxiv - Immunology 2021Quote: ... followed by streptavidin-HRP (3310-9-1000, Mabtech AB). The plates were developed with 1-Step Ultra TMB-ELISA Substrate Solution (34028 ...
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bioRxiv - Immunology 2021Quote: ... and visualization by Streptavidin horseradish peroxidase (HRP) (MABTECH Cat# 3310-9-1000).
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bioRxiv - Immunology 2020Quote: ... and incubated for 1 hour with streptavidin-HRP (diluted 1:100 with D-PBS, Mabtech, 3310-9-1000), and then washed again ...
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bioRxiv - Immunology 2023Quote: ... The CD40L-biotin was detected by adding a 1:1,000 dilution of Streptavidin-HRP (Mabtech, 3310-9-1000) and the signal was developed by addition of TMB substrate (BioLegend) ...
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bioRxiv - Immunology 2024Quote: ... Plates were washed 6x times with PBST and plated with streptavidin-HRP conjugate (1:1000 dilution; catalogue #3310-9-1000, Mabtech) for 1 hour at room temperature in the dark ...
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bioRxiv - Immunology 2020Quote: ... Plates were substrated for 8-12 minutes using 0.5µM filtered 3’3’5’5’-Tetramethylbenzidine (TMB substrate) (Mabtech, 3652-F10) and the plates were evaluated with an AID automated microplate ELISpot reader and associated software (AID ELISpot Version 7.0).
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bioRxiv - Immunology 2023Quote: ... TMB solution (MABTECH, 3651-10) was added followed by washing with deionized water after spots became obvious ...
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bioRxiv - Immunology 2023Quote: ... ELISpot plates (Mabtech, #3654-TP-10) were pre-wet with 15 μl/well of freshly prepared 35% ethanol for < 1 min at RT ...
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bioRxiv - Immunology 2021Quote: ... and IL-10 (MABTECH, 3432-4APW-2) ELISpotPLUS plates as well as Mouse IFNγ ELISpotPRO plates (MABTECH ...
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bioRxiv - Immunology 2020Quote: ... ALP-conjugated Streptavidin (Mabtech, Sweden, 3310-10) was added at 1:1000 ...
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bioRxiv - Immunology 2020Quote: ... and precipitating TMB substrate (Mabtech, #3651-10). Wells were activated with 15%(v/v ...
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bioRxiv - Immunology 2021Quote: ... ALP-conjugated streptavidin (Mabtech, Sweden, 3310-10) was added at 1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... IL-23 and IL-10 (MabTech AB), as well as IL-18 ...
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bioRxiv - Immunology 2023Quote: ... Plates were finally washed again with PBS+0.05% Tween-20 and spots were developed for 10 minutes with TMB substrate (Mabtech, 3651-10). Plates were rinsed with water and dried before counting the spots with EliSpot Reader ELR04 SR (AIDAutoimmun Diagnostika GmbH ...
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bioRxiv - Immunology 2021Quote: ELISpot assays were performed with mouse IFN-γ and IL-4 ELISpot PLUS kits according to the manufacturer’s instructions (3321-4HPT-10 and 3311-4HPW-10, Mabtech, Herndon, VA, USA). A total of 2,5 × 105 or 4 x 105 splenocytes from female mice were seeded per well for the IFN-γ and IL-4 tests respectively and ex vivo stimulated either with the 1:1 mix of the RBD overlapping peptides from the B.1.1.7 (alpha variant ...
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bioRxiv - Cancer Biology 2021Quote: ... coated with IFN-γ capture antibody (10 mg/mL; Mabtech) and incubated overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... the Monkey IFN-γ ELISpotPLUS kit (MABTECH 3421M-APT-10) was used as directed by the manufacturer ...
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bioRxiv - Immunology 2023Quote: ... and Monkey IL17A ELISpotPLUS Kit (Mabtech, Cat #3520M-4APW-10) were used following manufacturer’s instructions with 2×105 freshly isolated PBMC added to each well ...
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bioRxiv - Immunology 2022Quote: ... The 96-well ELISpot plates (MabTech, Cat# 3654-TP-10) were pre-wetted with 35% ethanol and washed five times with sterile water plus 1 time with PBS ...
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bioRxiv - Immunology 2021Quote: ... or monoclonal anti-human CD3 (Mabtech, Sweden, Cat. 3420-2AST-10) at a 1:1000 dilution for positive control wells ...
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bioRxiv - Immunology 2021Quote: Monkey IFN-γ ELISpotPro plates (Mabtech, Sweden, Cat#3421M-2APW-10) were prepared according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... we used antigen-specific IFN-γ ELISpot kits (MABTECH, 3321-4HPT-10) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... wells were washed and incubated with Streptavidin-ALP (Mabtech, Cat. 3310-10) for 1 hour ...
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bioRxiv - Immunology 2020Quote: ... Monkey IFN-γ ELISpotPro (alkaline phosphatase) plates (Mabtech, Sweeden, Cat#3421M-2APW-10) were blocked for a minimum of 2 hours with RPMI 1640 (Corning) ...
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bioRxiv - Immunology 2022Quote: ... IFNγ ELISPOT was performed using the Mouse IFNγ ELISPOTPLUS kit (MABTECH product # 3321-4HPW-10). ELISPOT plates were analyzed using ImmunoSpot CTL plate reader (CTL ImmunoSpot S6 Entry Analyzer ...
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bioRxiv - Immunology 2024Quote: ... then followed by addition of 100 µL/well of ELISpot substrate (catalogue #3651-10; Mabtech). Plates were incubated in the dark for up to 25 minutes while the spot formation was closely monitored ...
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bioRxiv - Immunology 2022Quote: ... we developed the spots by adding 70 µL/well of BCIP/NBT-plus substrate (Mabtech #3650-10) to the wells ...
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bioRxiv - Immunology 2022Quote: ... Anti-IFN-γ coating antibody (10 µg/ml in PBS; clone 1-D1K; Mabtech, Nacka Strand, Sweden) was then added and the plates incubated overnight at 4 °C ...
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bioRxiv - Immunology 2020Quote: ELISpot plates were coated with 10 μg/mL anti-human IFN-γ coating antibody (Mabtech, clone 1-D1K) and incubated overnight at 4C° ...
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bioRxiv - Immunology 2021Quote: ... cryopreserved PBMCs from study subjects were stimulated for 5 days with polyclonal B cell stimulation (1μg/mL R848 and 10 ng/mL rIL-2, Mabtech) in R10 media (RPMI 1640 with 10% FCS ...
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bioRxiv - Immunology 2023Quote: ... ELISpot assay plates were prepared and processed as per the manufacturer’s instructions (3321-4HPT-10, Mabtech Inc., Cincinnati, OH). ELISpots were enumerated by machine (CTL S6 Entry M2 ...
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bioRxiv - Immunology 2022Quote: ... PBMC were thawed and cultured at 37°C and 5% CO2 in the presence of 1 μg/mL R848 and 10 ng/mL of recombinant interleukin (IL) 2 (both from Mabtech).
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bioRxiv - Immunology 2022Quote: ... Splenocytes were counted and 250,000 cells were plated per well into pre-covered plates and stimulated overnight with 10 μg/mL SARS-CoV-2 (S1) peptide pool (Mabtech) or medium as negative control or PHA 2 μg/mL as positive experimental control ...
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bioRxiv - Microbiology 2022Quote: ... all at 10 µg/ml except for the CEF pool (CEF extended peptide pool for human CD8 T cells, Mabtech) used at 5 µg/ml and cultured overnight at 37°C ...
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bioRxiv - Immunology 2023Quote: ... plates were developed using reagents and following procedures and incubation periods described in the pig IFNγ ELISpot Plus kit (3130-4HPW-10, Mabtech). Spots were counted under a dissection microscope (Leica GZ6) ...
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bioRxiv - Immunology 2023Quote: ... Commercially available ELISPOT plates for evaluation of IL-4 (MuIL4, Immunospot, Cellular Technology Limited) and IFNg (MuIFNg, Immunospot, Cellular Technology Limited; cat# 3110-4APW-10, rabbit, MabTech) were used ...
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bioRxiv - Immunology 2023Quote: ... Wells were then washed again and spots were developed with filtered substrate solution BCIP/ NBT-plus (Mabtech, Cat. 3650-10). When distinct spots were visible ...
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bioRxiv - Immunology 2019Quote: ... was coated overnight at 4 °C with 10 μg ml−1solution of anti-IFN-γ monoclonal antibody (Mabtech clone 1-D1K) in a buffer solution of 0.1 M Na2CO3 ...
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bioRxiv - Biochemistry 2020Quote: ... serum samples were centrifuged at 10000 rpm for 20 minutes at 4 °C and supernatants were analyzed by human ApoA-I ELISA (3710-1HP-10, Mabtech Inc.), as well as by denaturant and native western blot (procedure is described in the Supplementary Information file).
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bioRxiv - Immunology 2022Quote: Incubate the array with 10 μg/mL biotinylated anti-human IFNγ 7-B6-1 (MABTECH AB, Stockholm, Sweden, Cat #3420-6-250) and then 12 μg/mL AF647-Streptavidin (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2021Quote: ... splenocytes or lung leukocytes were stimulated with 1 μg/ml R848 and 10 ng/ml recombinant human IL-2 (Mabtech In, OH). Millipore ELISPOT plates (Millipore Ltd ...
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bioRxiv - Microbiology 2021Quote: ... pulsed with WT1328-348 (10 µg/ml) followed by measurement of specific cytokine production using the human IFN-γ ELISpot kit (Mabtech AB) and ELIPHOTO Counter (Minerva Tech).
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bioRxiv - Immunology 2022Quote: ... were pre-wet with 30% ethanol for 60 seconds and then coated with 100 µl primary anti-IFN-γ antibody solution (10 µg/ml of 1-D1K coating antibody from Mabtech, Cincinnati, OH) OVN at 4°C ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 antigen RBD01-dn5B was diluted to 80 mg/mL and used to coat wells of PVDF plates (Mabtech, #3654-WP-10) according to the assay kit protocol ...
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bioRxiv - Immunology 2024Quote: ... were pre-wet with 30% ethanol for 60 seconds and then coated with 100 µl primary anti-IFN-γ antibody solution (10 µg/ml of 1-D1K coating antibody from Mabtech, Cincinnati, OH) OVN at 4°C ...
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bioRxiv - Immunology 2021Quote: ... washed with sterile PBS and coated overnight at 4 °C with anti–IFN-γ antibody (10 µg/ml in PBS; clone 1-D1K; Mabtech, Nacka Strand, Sweden). Prior to use ...
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bioRxiv - Microbiology 2024Quote: ... The diluted plasma was determined to determine IFN-γ levels using a commercial monkey IFN-γ ELISApro kit (catalog no. M4210M-1HP-10, Mabtech AB, Sweden), containing mAb MT126L ...
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bioRxiv - Immunology 2020Quote: ... were pre-wet with 70% methanol and then coated with 100 µl primary anti-IFN-γ antibody solution (10 µg/ml of 1-D1K coating antibody from Mabtech in PBS, pH 7.4, V-E4) OVN at 4°C ...