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Citations for PerkinElmer :
1 - 44 of 44 citations for Legionella pneumophila antigen native extract since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... and underwent antigen retrieval in antigen retrieval buffer (AR9, PerkinElmer) in a 95LC water bath ...
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bioRxiv - Microbiology 2021Quote: ... Gag p24 antigen concentration was measured with the Alliance HIV-1 p24 Antigen ELISA kit (Perkin Elmer).
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bioRxiv - Microbiology 2022Quote: ... by measuring the Gag p24 antigen concentration with the Alliance HIV-1 p24 Antigen ELISA kit (Perkin Elmer).
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bioRxiv - Microbiology 2022Quote: ... Concentration of HIV p24 antigen in each stock was determined by a p24 antigen capture kit (PerkinElmer, Waltham, MA, USA) and the concentration of total virus proteins were determined by a BCA protein assay kit (Thermo Fisher ...
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bioRxiv - Genomics 2020Quote: ... Antigen retrieval proceeded in AR6 buffer (AR600, PerkinElmer) at 100 °C for 23 min in a pressure cooker ...
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bioRxiv - Cancer Biology 2022Quote: ... Slides underwent antigen retrieval in AR9 Buffer (PerkinElmer, AR900250ML) for 1 min (100% Power ...
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bioRxiv - Microbiology 2022Quote: ... HIV-1 replication activity was determined by measuring p24 antigen levels in the culture supernatants using the p24 antigen capture assay (PerkinElmer, Boston, MA, USA) (23).
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bioRxiv - Immunology 2022Quote: ... Levels of p24 antigen in supernatant were quantified by p24 ELISA (PerkinElmer) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2019Quote: ... Antigen detection was performed using Tyramide signal amplification (Perkin Elmer-TSA-Plus Fluorescein) according to manufacturer’s instruction ...
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bioRxiv - Immunology 2022Quote: ... antigens were visualized using a Western Lightning Plus-ECL (Perkin Elmer, 50-904-9323).
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bioRxiv - Evolutionary Biology 2021Quote: ... cDNAs containing the mutated sites 155E->Q and 190E->Q and the native 155E190E cDNA were then sequenced on an ABI 3130 sequencer with the BigDye v.3.1 (Perkin Elmer) terminator chemistry to verify the sequences before overexpression.
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bioRxiv - Immunology 2019Quote: ... 14 and 21 for HIV-1 p24 antigen levels by ELISA (Perkin Elmer, Boston, MA).
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bioRxiv - Bioengineering 2021Quote: ... Heat-induced antigen retrieval (HIER) was carried out for all sections in AR6 buffer (AR6001KT, PerkinElmer), pH = 6.00 ...
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bioRxiv - Microbiology 2020Quote: ... Virus stocks were analyzed for HIV-1 p24 antigen by the AlphaLISA HIV p24 kit (PerkinElmer). Virus stocks contained approximate 200 ng/ml of HIV p24 protein.
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bioRxiv - Immunology 2023Quote: Serum samples were serially diluted and transferred onto an antigen-coated and blocked SpectraPlate-96 (PerkinElmer) plate ...
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bioRxiv - Cancer Biology 2021Quote: ... Antigen-antibody complexes were detected with West Pico Chemi luminescent substrate using standard manufactures protocol (Perkin Elmer). Imaging of all western blots was performed using an UVP BioImager 600 system equipped with VisionWorks Life Science software (UVP ...
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bioRxiv - Cell Biology 2019Quote: ... radioactivity in cell extracts was determined with MicroBeta2 liquid scintillation spectrometer (Perkin Elmer, Italy). Protein content in monolayers was determined directly in the filters using a modified Lowry procedure [21] ...
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bioRxiv - Immunology 2020Quote: ... The integrated signal density corresponding to labeled antigen or antibody in each LN was calculated using LivingImage (PerkinElmer) or ImageJ software and plotted using GraphPad Prism 8 ...
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bioRxiv - Microbiology 2021Quote: Concentration of HIV in each stock was determined by a p24 antigen capture kit (PerkinElmer, Waltham, MA, USA).
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bioRxiv - Plant Biology 2022Quote: ... The radioactivity in cell extracts were counted using liquid scintillation counting (Tri-Carb 2910TR, PerkinElmer). [3H]IAA retention is presented as residual radioactivity measured at 10 min relative to 0 min ...
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bioRxiv - Microbiology 2023Quote: The extracts were transferred to a 96-well microtiter dish (Black Isoplate®, Perkin Elmer) and left to evaporate ...
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bioRxiv - Immunology 2022Quote: ... Multiplex-immunofluorescence or confocal laser scanning microscopy imaging were performed after antigen retrieval with epitope retrieval buffer (PerkinElmer Inc.). Slides were incubated sequentially for 1h with the following antibodies ...
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bioRxiv - Immunology 2021Quote: ... The SARS-CoV-2 spike protein antigen (2.0µg/mL) was directly adsorbed on half-area 96-well OptiPlate (96W HB, Perkin Elmer) microplates for 2h at 37°C in a humidified incubator.
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bioRxiv - Microbiology 2019Quote: ... were infected with viral particles of Makona GP-A82 or V82 pseudoviruses in a 48 well plate using the same amount of p24 antigen quantified by AlphaLISA (AL291C:500 assay; PerkinElmer). After 48 h of incubation cells were washed twice with PBS and lysed for luciferase assay ...
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bioRxiv - Immunology 2020Quote: ... and then re-fixed in formalin for 15 minutes followed by antigen retrieval as per the manufacturer’s instructions (Perkin Elmer). Blocking was performed for 10 minutes ...
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bioRxiv - Pathology 2023Quote: ... the p24 core antigen was detected directly in the viral supernatant with a HIV-1 p24 ELISA kit (Perkin Elmer), as specified by the supplier ...
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bioRxiv - Microbiology 2022Quote: ... an equivalent of 300 ng of Gag p24 was lysed in ELISA lysis buffer containing 1% Triton (Alliance HIV-1 p24 Antigen ELISA kit, Perkin Elmer) and protease inhibitors for 30 min on ice ...
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bioRxiv - Plant Biology 2020Quote: ... The absorbance of chlorophyll containing acetone extract was taken immediately using spectrophotometer (Perkin Elmer, USA, Lambda-25) at 663 and 645 nm ...
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bioRxiv - Microbiology 2020Quote: ... The concentration of HIV-1 Gag p24 antigen in supernatants was determined with HIV-1 p24 ELISA kit (PerkinElmer, Inc. Waltham, MA). To analyze HIV-1 Env expression ...
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bioRxiv - Genetics 2022Quote: ... The physical titer of vector preparations was measured using the HIV-1 Gag p24 antigen immunocapture assay kit (PerkinElmer, Waltham, MA, USA) and expressed as p24 ng/ml ...
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bioRxiv - Cell Biology 2020Quote: ... Extracts (15 μg protein) were electrophoresed on SDS-PAGE and then transferred to polyvinylidene difluoride membranes (Perkin Elmer). The membranes were incubated overnight at 40C on rocker with primary antibodies in 5% milk dissolved in 1x PBS tween ...
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bioRxiv - Molecular Biology 2023Quote: One µL of each extract was injected in a Clarus 690 gas chromatograph (Perkin Elmer Inc., Wellesley, MA) coupled to a Clarus SQ8T MS instrument operating in full scan mode and using EI (70 eV) ...
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bioRxiv - Plant Biology 2019Quote: ... 200 uL from the methanol:chloroform extract was then combined with 5 mL of scintillation fluid (HIONIC FLUOR, Perkin Elmer). The remaining extract was removed from the residual biomass and water (2 mL ...
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bioRxiv - Evolutionary Biology 2021Quote: ... frigida male CHC extract was injected splitless into a gas chromatograph (GC) Clarus 500 (Perkin Elmer, Shelton, CT, USA) equipped with an Elite-5 column (30 m × 0.25 mm× 0.25 µm ...
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bioRxiv - Microbiology 2022Quote: ... 10 μL of lipid extracts were mixed with 2 mL of Ultima Gold™ scintillation fluid (Perkin Elmer, USA), and radioactive counts were measured using a MicroBeta2® scintillation counter (Perkin Elmer ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: The GC-MS analysis of the effective extracts was performed using Clarus 680 GC (Perkin Elmer. U. S. A) and the GC interfaced to a mass spectrometer (GC-MS ...
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bioRxiv - Genomics 2023Quote: ... The metabolite extract was transferred into scintillation vials with 5 mL Insta-Gel Plus scintillation cocktail (Perkin Elmer #601339).
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bioRxiv - Microbiology 2021Quote: ... The level of HIV-1 replication was determined by measuring p24 antigen in the culture supernatant using a kit (Perkin Elmer Inc, Shelton, CT, USA).
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bioRxiv - Microbiology 2020Quote: ... Extracts were analyzed by gas chromatography coupled with mass spectrometry (Clarus SQ 8T GC/ Mass Spectrometer, Perkin Elmer, Walthman, MA, USA). The NIST mass-spectral library identifications were confirmed with chemical standards when available (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were harvested after 48 hr transfection and nuclear extracts were prepared as previously described.78 The two strands of short probe was labeled with [γ-32P]-adenosine triphosphate (PerkinElmer). After that ...
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bioRxiv - Plant Biology 2020Quote: Quantification of constituents in extracts was performed using a GC-FID system comprising a Perkin Elmer Autosystem XC (Perkin Elmer, Melbourne, Australia) fitted with a low polarity Zebron ZB-5 (30 m × 250 μm i.d. ...
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bioRxiv - Molecular Biology 2023Quote: ... 6 ng/ µl recombinant TopBP1 protein or buffer were added to TopBP1 immunodepleted interphase Xenopus egg extract containing 50 nCi/µl α32P-dCTP (Perkin Elmer NEG513A250UC). To start the replication reaction the egg extracts were supplemented with 5 ng sperm DNA (kindly provided by the lab of O ...
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bioRxiv - Molecular Biology 2023Quote: DNA methylation of replicating sperm or erythrocyte nuclei in egg extract was assayed by the incorporation of 3H-SAM (S-[methyl-3H]-adenosyl-L-methionine; Perkin Elmer, NET155H). Demembranated sperm nuclei were prepared as published previously (86) ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA libraries were prepared from RNA extracts with a minimum RNA integrity number (RIN) of 9.5 using the NEXTFLEX® Combo-Seq mRNA/miRNA Kit (PerkinElmer®, Waltham, MA). Libraries were quantified using a KAPA Library Quantification Kit (Roche Sequencing and Life Science ...