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Citations for PerkinElmer :
1 - 50 of 50 citations for Human ADH5 siRNA since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... transfection complex was prepared by mixing 50 nM of SMARTPool ON-TARGETplus Human siRNAs (horizon, PerkinElmer) with 3 μl of Lipofectamine RNAiMAX transfection reagent (ThermoFisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... ON-TARGETplus Human NFE2L2 siRNA SMARTPool and ON-TARGETplus Non-targeting Control Pool (Horizon Discovery, Perkin Elmer) were used ...
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bioRxiv - Biochemistry 2021Quote: ... an optimized set of 4 small interfering RNAs (siRNAs; SMARTPool) targeted against human Furin were purchased from Horizon Discoveries (Perkin Elmer, Lafayette, LA, USA) and transfections of HeLa cells were carried out using INTERFERin (PolyPlus ...
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bioRxiv - Molecular Biology 2021Quote: ... siRNAs and shRNAs were purchased from Dharmacon (PerkinElmer, Waltham, MA) and Santa Cruz Biotechnology (Dallas ...
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bioRxiv - Cell Biology 2024Quote: The siRNA library was spotted in 384-well black μClear plates (PerkinElmer). siRNAs were transiently transfected at a final concentration of 25 nM per well using 0.25 μl Lipofectamine RNAiMAX reagent in 7.25 μl of OptiMEM medium ...
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bioRxiv - Neuroscience 2021Quote: siRNA was dispensed into Geltrex-coated 96-well CellCarrier Ultra plates (Perkin Elmer) at a final concentration of 30 nM using the Echo 555 acoustic liquid handler (Labcyte) ...
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bioRxiv - Neuroscience 2024Quote: ... reverse transfection of siRNA and miRNA was performed in 96-well (PerkinElmer, 6055308) or 384-well (Greiner ...
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bioRxiv - Cancer Biology 2023Quote: ... Human IL-2 concentration was measured with the AlphaLISA human IL2 Kit (PerkinElmer) and data collected on an Envision Plate Reader (PerkinElmer) ...
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bioRxiv - Molecular Biology 2024Quote: siRNA-transfected or Dexamethasone-synchronized cells were plated in 96-well Cell Carrier Ultra plates (Perkin Elmer). At the end of DSB inducing treatment ...
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bioRxiv - Cancer Biology 2022Quote: ... 2.5μl of 500nM siGENOME siRNA smart pool and 2.5μl Opti-MEM per well was added into CellCarrier Ultra 384 well plates (Perkin Elmer). 4.95μl Opti-MEM was incubated with 0.05μl Dharmafect IV for 5min ...
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bioRxiv - Cancer Biology 2024Quote: ... 2.5µl of 500nM siGENOME siRNA and 2.5µl Opti-MEM per well were added into CellCarrier Ultra 384-well plates (Perkin Elmer). 4.95µl Opti-MEM was incubated with 0.05µl Dharmafect IV for 5 minutes ...
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bioRxiv - Immunology 2023Quote: ... using anti-human IFN-γ capture antibody (Pharmingen, 551221), biotinylated anti-human IFN-γ detection antibody (Pharmingen, 554550) and DELFIA Europium-labelled streptavidin (PerkinElmer), for fluorescent detection and quantification.
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bioRxiv - Cell Biology 2021Quote: ... 500nl of 2 µM siRNAs (20nM final concentration) were distributed on 6 different 384-well imaging plates (CellCarrier Ultra, Perkin Elmer), as described in Table 3 ...
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bioRxiv - Cell Biology 2022Quote: ... and 150 nl of each diluted siRNA were spotted at the bottom of 384-well CellCarrier Ultra imaging plates (PerkinElmer, #6057300) using the ECHO525 acoustic liquid dispenser (Labcyte) ...
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bioRxiv - Physiology 2022Quote: Gene silencing was performed according to the Lipofectamine RNAiMAX® transfection protocol using 25 nM SMART pool ON-TARGETplus siRNAs (PerkinElmer, USA/UK ...
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bioRxiv - Molecular Biology 2023Quote: HEK293T cells were seeded and 24 hours later transfected with 50 nM of siPOOLs targeting RPL24 or non-targeting siPOOLs as control (ON-TARGETplus siRNA, Horizon, Perkin Elmer) using HiPerFect transfection reagent (301705 ...
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bioRxiv - Neuroscience 2023Quote: ... Alpha-LISA kits specific for human Aβ1–X (AL288C, PerkinElmer) and Aβ1–42 (AL276C ...
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bioRxiv - Neuroscience 2020Quote: The AlphaLISA human frataxin detection kit (PerkinElmer, catalogue # AL322HV/C/F) was scaled down to a white opaque 384-well plate format (PerkinElmer ...
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bioRxiv - Cell Biology 2021Quote: Primary human hepatocytes were seeded in 96-well black CellCarrier plates (PerkinElmer) at a density of 5×103 cells per well ...
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bioRxiv - Immunology 2021Quote: Human fibroblasts cells were seeded on CellCarrier-96well Ultra microplate (Perkin Elmer) and incubated for at least 24 hours in growth media ...
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bioRxiv - Biochemistry 2023Quote: 5x104 human neutrophils were seeded in a black 96-well plate (PerkinElmer) in HyClone HBSS +Ca ...
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bioRxiv - Cell Biology 2021Quote: The human glioblastoma U87 MG-Red-FLuc (Bioware Brite, PerkinElmer, Waltham, Massachusetts, USA) was the authorized cell line in all experiments ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Multispectral microscopy of the human biopsies was performed using a Vectra 3.0 (PerkinElmer) equipped with 20x and 40x objectives ...
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bioRxiv - Immunology 2020Quote: ... Bound IgG was detected with Eu-N1 Anti-Human IgG (Perkin Elmer, 1244-330). using DELFIA-TRF system (Perkin Elmer).
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bioRxiv - Genomics 2021Quote: Human primary AMSCs and PHH were plated in 96-well CellCarrier plates (Perkinelmer #6005550). AMSCs were differentiated for 14 days and high content imaging was performed at day 0 ...
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bioRxiv - Cell Biology 2021Quote: Human Fibroblasts Cells were seeded on CellCarrier-384 or 96well Ultra microplate (Perkin Elmer) and incubated for at least 24 h in growth media ...
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bioRxiv - Neuroscience 2020Quote: ... mammalian CHO K1 cells stably overexpressing apo-aequorin and human Gα16 (ES-000-A24, PerkinElmer) were transiently transfected with Ce-npr-4a/pcDNA3.1 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... CHO cells stably expressing the human CB1 receptor were obtained from PerkinElmer (#ES-110-C) and utilized for western blots (CB1-CHO-C2 ...
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bioRxiv - Immunology 2023Quote: ... for labeling human lymphocytes with or without inhibitors and for the [3H]-Leu (Perkin Elmer) ribosome transit analysis ...
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bioRxiv - Cell Biology 2024Quote: Human islets were dissociated and plated as above in 96 well plates (Perkin Elmer, 6055302) and were infected with MOI 1 of lentivirus expressing shRNA for 48 hours before drug treatment ...
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bioRxiv - Biochemistry 2021Quote: ... Fully-haploid engineered HAP1 (eHAP) human cells (Essletzbichler et al., 2014) were obtained from Horizon (now PerkinElmer), and were cultured in complete IMDM medium (ThermoFisher # 12440-053 ...
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bioRxiv - Genomics 2021Quote: All fluorescence imaging on human kidney tissue was performed using the Opera Phenix High-Content Screening System (PerkinElmer). 5mm cryosections were placed into individual wells of 24-well plates and the entire specimen initially imaged for DAPI at 5X ...
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bioRxiv - Immunology 2024Quote: ... supernatants were collected and IL-1β levels were measured using the Human IL-1β HTRF kit (Cisbio – PerkinElmer), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: The level of MMP-9 in plasma was measured with an AlphaLISA kit for the human protein (#AL3138, PerkinElmer). AlphaLISA signals were measured using an Ensight Multimode Plate Reader (PerkinElmer).
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bioRxiv - Genomics 2021Quote: ... Insulin was quantified using the Insulin (human) AlphaLISA Detection Kit and the EnSpire Alpha Plate Reader (both Perkin Elmer) based on 1:10 and 1:200 dilutions for supernatant and insulin content ...
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bioRxiv - Immunology 2024Quote: ... Clone 2C.4 was screened against human cardiac myosin and the β-adrenergic receptor (βAR) enriched membranes (Perkin Elmer) by ELISA ...
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bioRxiv - Genomics 2021Quote: Two-color FISH experiments were performed using human fosmid (n=2) or BAC (n=2) clones directly labelled by nick-translation with Cy3-dUTP (PerkinElmer) and fluorescein-dUTP (Enzo ...
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bioRxiv - Immunology 2020Quote: ... during the final 6 hours (murine MLR) or 15 hours (human MLR) of culture was measured by a TopCount (PerkinElmer).
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bioRxiv - Biochemistry 2023Quote: [125 I]-Bolton-Hunter labeled Transforming Growth Factor- β1 (Human, Recombinant) (catalog NEX267) was obtained from Perkin Elmer (Waltham, MA) This product was however discontinued in 2019 ...
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bioRxiv - Bioengineering 2023Quote: ... overexpressing the human FGFR1c together with β-klotho was plated into 384-well micro-titer plates (Perkin Elmer, Cat. #6007480) and incubated at 37°C ...
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bioRxiv - Cell Biology 2024Quote: Human islets were infected with adenovirus as described above and were imaged in 96 well imaging plates (Perkin Elmer, 6055302) using Opera Phenix Plus High Throughput Confocal and images were analyzed using the Harmony High Content Imaging and Analysis software.
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bioRxiv - Immunology 2024Quote: ... diluted 1:40 in assay diluent 5 and then IL-1β levels were measured using the Human IL- 1β HTRF kit (Cisbio – PerkinElmer), following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Radioligand binding assays were performed with membranes prepared from cells expressing human CB2 or CB1 or mouse CB2 receptor using [3H]-CP55940 (Perkin Elmer) as a radioligand ...
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bioRxiv - Immunology 2020Quote: We stained 5-µm-thick formalin-fixed paraffin-embedded sections from human xenograft tumors (UMSCC-1) using Opal multiplex kits (PerkinElmer/Akoya), for a panel of DAPI ...
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bioRxiv - Microbiology 2023Quote: Apical wash fractions collected pre- and post-infection were used to detect Lactate dehydrogenase activity and IL-8 concentrations using human LDH and IL-8 Alphalisa Kits (Perkin Elmer). ELISA Kits for the detection of human TNFα ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... M311A and S315A mutations were introduced in a plasmid coding for the human V2R sequence fused at its N-terminus to the enzyme-based self- labeling SNAP-tag (pRK5-SNAP vector, PerkinElmer Revvity). For each mutation ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cells were transfected 24 h later with the pRK5 plasmid coding for the human V2R (wild-type or mutant) fused at its N-terminus to the SNAP-tag (pRK5-SNAP vector, PerkinElmer Revvity). Transfections were performed with X-tremeGENE 360 (Merck) ...
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bioRxiv - Immunology 2024Quote: ... Five-micrometer serial sections were stained with a human-specific anti-COXIV antibody and scanned using the Vectra® Polaris Automated Imaging System (PerkinElmer, USA) at 20×magnification ...
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bioRxiv - Systems Biology 2024Quote: ... The NPCs were cultured in a 12-well dish to confluency in NPC media and then dissociated using Accutase for 5 minutes at 37°C to single cell resolution and plated on 5 µg/mL human laminin and 5µg/mL fibronectin-coated 384 well plates (Perkin Elmer Cat #6057308) at 12,000 cells/well in NPC media and 10 µM ROCKi ...
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bioRxiv - Cell Biology 2021Quote: ... hand-picked human islets were dispersed in accutase for 10 min at 37°C and plated in 96-well plates (Perkin Elmer Inc., Waltham, MA) treated with Poly-D-Lysine Hydrobromide (Sigma-Aldrich ...