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Citations for CisBio :
1 - 49 of 49 citations for 7 Bromo 2 methylimidazo 4 5 c pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... cAMP concentrations were measured from cell lysates after cells were incubated for 5 minutes with synthetic agonists (10 μM) for FFAR2 (4-CMTB) or FFAR3 (AR420626) using the HTRF cAMP Dynamic 2 immunoassay kit (CisBio). Fluorescence was measured with a PHERAstar FSX plate reader (BMG Labtech ...
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bioRxiv - Biophysics 2024Quote: ... Labeling with the Tb-cryptate for the LRET experiments was carried out by incubating the β-arrestin 1 with 2 equivalents of Lumi-4 Tb maleimide (CisBio) overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Eu-Protein A (5 nM, Cisbio), Streptavidin-Alexa Fluor 647 (6.25 nM ...
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bioRxiv - Immunology 2022Quote: ... Eu-Protein A (5 nM, Cisbio), Streptavidin-Alexa Fluor 647 (6.25 nM ...
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bioRxiv - Cell Biology 2021Quote: ... 16.7 mM glucose and 45mM KCl treatments were collected and insulin amount determined using a human insulin HTRF assay (Cisbio). Cells infected with lentivirus were incubated for 6 days prior to GSIS assay ...
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bioRxiv - Microbiology 2022Quote: ... 4 µl of 16× SAH-d2 conjugate solution (Cisbio) was added ...
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bioRxiv - Bioengineering 2021Quote: ... 4 μl of 16X SAH-d2 conjugate solution (CisBio) was added ...
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bioRxiv - Biochemistry 2021Quote: ... 4 μl of 16X SAH-d2 conjugate solution (CisBio) was added ...
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bioRxiv - Bioengineering 2021Quote: ... 2 μl Detection Buffer 1 (CisBio) was immediately added to the reaction mixture ...
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bioRxiv - Biochemistry 2021Quote: ... 2 μl Detection Buffer 1 (CisBio) was immediately added to the reaction mixture ...
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bioRxiv - Bioengineering 2021Quote: ... to 49 parts Detection Buffer 2 (CisBio).
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bioRxiv - Biochemistry 2021Quote: ... to 49 parts Detection Buffer 2 (CisBio).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... onto the C-terminus end of the SNAP-GLP-1R vector (Cisbio), followed by site-directed mutagenesis of the GLP-1R stop codon ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... onto the C-terminus end of the SNAP-GLP-1R vector (Cisbio), followed by site-directed mutagenesis of the GLP-1R stop codon ...
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bioRxiv - Cell Biology 2022Quote: ... onto the C-terminus end of the SNAP-GLP-1R vector (CisBio), followed by site-directed mutagenesis of the GLP-1R stop codon ...
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bioRxiv - Neuroscience 2023Quote: ... phospho- and total ERK1/2 were determined using the two-plate adherent cell protocol (Phosph-ERK1/2 kit, Cisbio), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The reaction was quenched by the addition of 2 µl of 5M followed by the addition of 2 µl Detection Buffer 1 (Cisbio) to the reaction mixture ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... HTRF detection reagents (cAMP Dynamic 2 kit, Cisbio) were added at the end of the incubation ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... ERK1/2 phosphorylation was determined by HTRF (Cisbio Phospho-ERK [Thr202/Tyr204] cellular kit ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cAMP was quantified by HTRF (cAMP Dynamic 2, Cisbio), and responses were normalised to that of forskolin ...
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bioRxiv - Cell Biology 2021Quote: ... Supernatants and lysates were stored at -30°C prior to HTRF assay (Mouse ultrasensitive, Cisbio).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cAMP was then assayed by HTRF (Cisbio cAMP Dynamic 2) using a Spectramax i3x plate reader (Molecular Devices) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cAMP was then assayed by HTRF (Cisbio cAMP Dynamic 2) and concentration-response curves constructed with 3-parameter curve fitting in Prism 8.0.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and cAMP determined by HTRF (cAMP Dynamic 2 kit, Cisbio) using a Spectramax i3x plate reader (Molecular Devices).
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bioRxiv - Neuroscience 2021Quote: ... which carried a C-terminal N-acyl-benzimidazolinone (Nbz) moiety for thioester formation and was synthesized by CisBio. Desulfurization of the ligation products yielded the corresponding Httex1 proteins with a ligation scar (Q18A) ...
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bioRxiv - Pathology 2022Quote: Cardiomyocytes cAMP concentration was assessed with cAMP dynamic 2 kit (Cisbio, Saclay, France), according to the manufacturer instructions.
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bioRxiv - Physiology 2022Quote: ... GLP-1R or GIPR using the cAMP Dynamic 2 cAMP kit (Cisbio, France) as per manufacturer’s instructions.
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bioRxiv - Biochemistry 2024Quote: ... The 5 µl of reaction mixture was transformed to HTRF 96 well low volume plates (Cisbio) and the consumption of ATP was measured with ADP-Glo Kinase Assay kit (Promega Corporation ...
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bioRxiv - Cell Biology 2022Quote: ... followed by lysis and cAMP HTRF immunoassay (cAMP Dynamic 2, 62AM4PEB, Cisbio, Codolet, France) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... After incubation for 1 hour at 37°C the IP1 accumulation was stopped by addition of 40 μL CisBio IP-One Tb HTRF Kit (CisBio, 62IPAPEC) lysis buffer ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and cAMP was determined by homogenous time-resolved fluorescence (HTRF, cAMP Dynamic 2 kit, Cisbio).
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bioRxiv - Cell Biology 2023Quote: ... 2 mM EGTA) and insulin content was measured by insulin ultra-sensitive assay kit (Cisbio). Within an experiment ...
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bioRxiv - Cell Biology 2022Quote: ... The samples were stored at -20°C until the insulin concentration was determined using an Insulin Ultra-Sensitive HTRF Assay kit (62IN2PEG, Cisbio, Codolet, France) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2022Quote: ... A 36 μl aliquot of this lysate was subsequently transferred to a 384 well plate containing 4 μl of the antibody solution (1:50 dilution of anti-ELP1 d2(CisBio) and anti-ELP1 K(CisBio ...
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bioRxiv - Cell Biology 2020Quote: Intracellular cAMP and IP1 was determined by HTRF (cAMP Dynamic 2 and IP-one, respectively (CisBio)) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... After stimulation cells were lysed and cAMP was assayed by immunoassay (Cisbio HTRF cAMP Dynamic 2).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ERK1/2 phosphorylation in HEK293 FPR cell systems was measured with the HTRF immunoassay from Cisbio, as described previously [37] ...
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bioRxiv - Cell Biology 2022Quote: ... dispersed islets were lysed and cAMP assayed by HTRF immunoassay (cAMP Dynamic 2, 62AM4PEB, Cisbio, Codolet, France).
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bioRxiv - Bioengineering 2021Quote: ... 16X SAH-d2 was prepared by adding one part SAH-d2 to 15 parts Detection Buffer 2 (CisBio).
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bioRxiv - Biochemistry 2021Quote: ... 16X SAH-d2 was prepared by adding one part SAH-d2 to 15 parts Detection Buffer 2 (CisBio). After 5 minutes ...
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bioRxiv - Neuroscience 2022Quote: The accumulation of cAMP was determined using a cAMP dynamic 2 kit (Cisbio, Bioassays, Bagnols sur-Cèze, France). Synaptosomes were incubated for 1 h at 37 ºC (0.67 mg/ml ...
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bioRxiv - Systems Biology 2024Quote: ERK1/2 phosphorylation in HEK293 cells was measured with the HTRF (Homogeneous Time-Resolved Fluorescence) immunoassay from Cisbio, as described previously [23] ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 5,6,7,8-tetrahydro-6-[(2-phenylethyl)propylamino]) derivative labeled with a red fluorescent probe (PPHT-red) was obtained from Cisbio Bioassays (Bagnolssur-Cèze ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and homogenous time-resolved fluorescence (HTRF) reagents for cAMP (cAMP Dynamic 2) and insulin (Insulin High Range Assay) measurement were obtained from Cisbio. SNAP-Surface probes were obtained from New England Biolabs ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Resuspended cells were stimulated at 37°C in their respective serum-free medium in 96-well half area opaque white plates before addition of HTRF lysis buffer and detection reagents (cAMP Dynamic 2 kit, Cisbio). The duration of stimulation and inclusion or not of the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were then lysed using the supplied lysis buffer and assayed for ERK1/2 phosphorylation using the phospho-ERK (Thr202/Tyr204) Cellular Assay Kit (Cisbio). Plates were read using a Mithras LB 940 multimode microplate reader (Berthold Technologies ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Lumi4-Tb-labelled ACE2 cells were incubated with d2-labelled anti-FLAG tag antibody (2 μg/mL, 1h at room temperature; 61FG2DLF, Cisbio Bioassays), followed by addition of non-labelled RBD (5 nM ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Changes in intracellular cellular cAMP were determined using a modified 2-step High-Throughput Time Resolved Fluorescence (HTRF) cAMP kit (Cisbio Bioassay, Codolet, France). Confluent HEK or EnSC cultures in 6-well plates were down-regulated prior to stimulation and assayed in Krebs-Henseleit Buffer (KHB ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ERK1/2 phosphorylation was measured using the homogeneous time resolved fluorescence (HTRF)® Phospho-ERK (T202/Y204) Cellular Assay Kit (Cisbio Bioassays, Codolet, France) two-plate format in accordance with the manufacturer’s instructions ...