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• Global proteomic profiling of primary macrophages during M. tuberculosis infection identifies TAX1BP1 as a mediator of autophagy targeting

  Jonathan M Budzik, et al., bioRxiv - Microbiology 2019

  Quote: Immunostaining for LC3 was performed with blocking and permeabilization buffer (0.3% Triton X-100, 2% bovine serum albumin) and anti-LC3 (clone 2G6, Nanotools #0260-100/LC3-2G6) at a dilution of 1:200 for 3 hours at room temperature ...
• Enhanced autophagic-lysosomal activity and increased BAG3-mediated selective macroautophagy as adaptive response of neuronal cells to chronic oxidative stress

  Debapriya Chakraborty, et al., bioRxiv - Cell Biology 2019

  Quote: ... LC3B (Nanotools, 0260-100), LC3B (Sigma ...
• NECAB2 participates in an endosomal pathway of mitochondrial stress response at striatal synapses

  Diones Bueno, et al., bioRxiv - Neuroscience 2023

  Quote: ... 1:100 (NanoTools, Teningen, Germany), anti-PDI ...
• Protein content and lipid profiling of isolated native autophagosomes

  Daniel Schmitt, et al., bioRxiv - Biochemistry 2021

  Quote: ... Primary antibody: MAP1LC3B (Nanotools, 0260-100). Secondary antibody ...
• ATG9 vesicles comprise the seed membrane of mammalian autophagosomes

  Taryn J. Olivas, et al., bioRxiv - Cell Biology 2022

  Quote: ... clone 2G6 (Nanotools; 0260100; mouse; 1:100), anti-ATG9A (Abcam ...
• A conserved ATG2-GABARAP interaction is critical for phagophore closure

  Mihaela Bozic, et al., bioRxiv - Cell Biology 2019

  Quote: ... anti-LC3B (clone 5F10 Nanotools, 0231-100/LC3-5F10) and anti-GABARAP (Abcam ...
• Temporal inhibition of autophagy reveals segmental reversal of aging with increased cancer risk

  Liam Cassidy, et al., bioRxiv - Cancer Biology 2019

  Quote: ... Anti-LC3 (Nanotools, LC3-5F10 0231-100, 1:400).
• B cell-intrinsic requirement for WNK1 kinase in T cell-dependent antibody responses

  Darryl Hayward, et al., bioRxiv - Immunology 2021

  Quote: ... injection of anti-CD62L (Mel-14, 100 µg/mouse; Nanotools) in combination with Alexa Flour 633-conjugated anti-PNAd (MECA-79 ...
• KANSL1 Deficiency Causes Neuronal Dysfunction by Oxidative Stress-Induced Autophagy

  Katrin Linda, et al., bioRxiv - Neuroscience 2020

  Quote: ... mouse anti-LC3 (1:500; NanoTools 0231-100/LC3-5F10); rabbit anti-LAMP1 (1:200 ...
• KANSL1 Deficiency Causes Neuronal Dysfunction by Oxidative Stress-Induced Autophagy

  Katrin Linda, et al., bioRxiv - Neuroscience 2020

  Quote: ... for LC3 probing (1:200; NanoTools 0231-100/LC3-5F10), PVDF membrane (BioRad) ...
• Targeted proteomics addresses selectivity and complexity of protein degradation by autophagy

  Alexandre Leytens, et al., bioRxiv - Cell Biology 2024

  Quote: ... anti-LC3 (Nanotools, 0231-100/LC3-5F10; Novus, NB100-2220), anti-TAX1BP1 (Sigma ...
• p27 regulates the autophagy-lysosomal pathway via the control of Ragulator and mTOR activity in amino acid deprived cells

  Ada Nowosad, et al., bioRxiv - Cell Biology 2020

  Quote: ... Mouse anti LC3B (0231-100/LC3-5F10) was purchased from NanoTools. Secondary antibodies against whole Ig or Ig light-chain conjugated to horseradish peroxydase or Cyanine-2 and −3 were purchased from Jackson ImmunoResearch ...
• Genome-wide screen reveals Rab12 GTPase as a critical activator of pathogenic LRRK2 kinase

  Herschel S. Dhekne, et al., bioRxiv - Biochemistry 2023

  Quote: ... Mouse anti-total Rab10 (0680–100/Rab10-605B11, Nanotools or ab104859, Abcam), Rabbit anti-pS106 Rab12 (ab256487 ...
• Leucine Rich Repeat Kinase 2 is not required for lysozyme expression in intestinal Paneth cells

  Anna Tasegian, et al., bioRxiv - Physiology 2024

  Quote: ... The total Rab10 mouse antibody was from NanoTools (0680–100/ Rab10-605B11). Rabbit monoclonal antibody for LRRK2 phospho-Ser935 (#UDD2 ...
• An unconventional autophagic pathway that inhibits ATP secretion during apoptotic cell death

  Elena Terraza-Silvestre, et al., bioRxiv - Cell Biology 2024

  Quote: ... Primary antibodies were: anti-LC3 (1/200, 5F10, mouse mAb, NanoTools 0231-100) or anti-AU (1/1000 ...
• Endogenous Rab29 does not impact basal or nigericin and monensin stimulated LRRK2 pathway activity

  Alexia F. Kalogeropulou, et al., bioRxiv - Neuroscience 2020

  Quote: ... The mouse monoclonal anti-Rab10 total antibody was purchased from Nanotools (#0680–100/Rab10-605B11) and used at a final concentration of 1 μg/ml ...
• Mitophagy contributes to alpha-tocopheryl succinate toxicity in GSNOR-deficient hepatocellular carcinoma

  Salvatore Rizza, et al., bioRxiv - Cancer Biology 2019

  Quote: ... incubated for 1 h in blocking solution and then over night with an anti-Microtubule-associated proteins 1A/1B light chain 3B (anti-LC3, 0231-100, Nanotools) and anti-TOM20 (Santa Cruz Biotechnology ...
• VE-PTP participates in vasculogenic mimicry by preventing autophagic degradation of VE-cadherin

  Daniel Delgado-Bellido, et al., bioRxiv - Cancer Biology 2019

  Quote: ... we performed western blot of LC3-I and its proteolytic derivative LC3-II (18 and 16 kDa, respectively) using a monoclonal antibody against LC3 (NanoTools, clone 5F10, Ref 03231-100/LC3-5F10).