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Citations for Cayman Chemical :
401 - 450 of 468 citations for 5 Bromo 2 bromo difluoro methyl 1H benzimidazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... zebrafish embryos were placed per well on 12-well plates containing 3 mL embryo water plus 5 μM or 7 μM Erdafitinib (JNJ-42756493, Item No. 21813, Cayman Chemical); or 5 μM or 20 μM BCI (hydrochloride ...
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bioRxiv - Cancer Biology 2022Quote: ... MDA-231 or BT-549 cells were treated with 2 μM NVP-BKM120 (Cayman Chemical, CAS #944396-07-0), 5 μM of Afuresertib (GSK2110183 ...
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bioRxiv - Immunology 2021Quote: ... the membrane was incubated overnight at 4°C with the primary antibodies anti-COX-2 (1:200, Cayman Chemical) and anti-β-actin loading control (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... 20 µL 10 mM N-[6-(biotinamido)hexyl]-3′-(2′-pyridyl dithio)propionamide (biotin-HPDP, Cayman Chemical Company, 16459) in DMSO was added to each sample ...
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bioRxiv - Cancer Biology 2022Quote: ... Arp 2/3 complex inhibitor (CK-666≤200μM) and CDC42 inhibitor (ML-141≤20μM) were obtained from Cayman Chemical. The PI3K pathway inhibitor (LY294002≤100μM) ...
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Characterization of an evolutionarily distinct bacterial ceramide kinase from Caulobacter crescentusbioRxiv - Biochemistry 2023Quote: ... we performed the reaction under identical conditions using 1-NBD-decanoyl-2-decanoyl-sn-Gly (NBD-DAG) (Cayman Chemical) as the substrate ...
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bioRxiv - Microbiology 2023Quote: ... MTT (3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide) assay was performed using an MTT assay kit (Cayman Chemical). Cells in 96-well plates were treated with DMSO or desired chemical inhibitors at different concentrations and incubated for desired time ...
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bioRxiv - Biochemistry 2023Quote: Thin films were prepared by dissolving 1-palmitoyl-2-oleoyl-sn-glycero-3-PC (POPC) and cholesterol (Cayman Chemical) in chloroform to create 50 mg/mL solutions that were partitioned into amber glass vials ...
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bioRxiv - Microbiology 2024Quote: ... The following reagents were added at the indicated concentrations unless otherwise noted: 2-deoxyglucose [10 mM (Cayman Chemical Company)] ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were treated with Erastin(10 μM) for 6 or 18 hours or RSL3(2 μM)(Cayman Chemicals) for 2 hours in the presence and absence of Liproxstatin (1 μM) ...
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bioRxiv - Biochemistry 2024Quote: ... Crystals of the dPCoA complex were obtained by adding 0.8 µL 6.7 mg mL-1 CarI TAD Δhelix protein containing 2 mM dPCoA (Cayman Chemical) to 0.4 µL 100 mM bis-tris propane pH 8.5 ...
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bioRxiv - Neuroscience 2022Quote: We validated our physiologically compatible THC solution (1:1:18 THC:cremophor:saline at 2.5, 5, or 10 mg/kg of THC; Cayman Chemicals, Ann Arbor, MI, USA) by measuring plasma levels of THC and two THC metabolites (11-hydroxy-delta-9-THC and 11nor-9carboxy delta-9-THC ...
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bioRxiv - Molecular Biology 2021Quote: ... A metabolic labeling protocol was followed[6] and the experimental HEK293 cells were incubated in the presence of 40µM unphosphorylated 4-thiouridine (Cayman Chemical, CAY16373-5). Total RNA was harvested from the cells using Trizol (Ambion ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The experiment was stopped by the addition of 5% trichloroacetic acid (TCA) and the concentration of cGMP was measured using the cGMP enzyme immuno assay (EIA) kit (Cayman Chemical Company).
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bioRxiv - Immunology 2022Quote: ... MAIT cells were stimulated by APCs pulsed 3 h with 5-OP-RU as previously described (83) or with 30 µM 6,7-dimethyl-8-Ribityllumazine (Cayman Chemical Cat#23370).
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bioRxiv - Biochemistry 2023Quote: ... cell lysates were incubated for 5 min with competitor (Arachidonic acid, docosanoic acid, Cayman Chemical 9000338, or 8-HETE, Cayman Chemical 34340) at 37°C before addition of other substrates and initiation with NADPH.
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bioRxiv - Biochemistry 2023Quote: Purified human PPP1R15A single repeat peptides (UK2997, UK3098) were labelled with 10-fold molar concentration of fluorescein-5-malamide (16383, Cayman Chemical Company) in the presence of 1 mM TCEP at 4 °C overnight ...
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bioRxiv - Biochemistry 2024Quote: Glioblastoma samples were prepared with the following matrix application protocol: 100 µL of a 5 mg/mL deuterated tryptophan (D5-Trp) solution (Cayman Chemicals, 34829) in ACN/H2O (50:50 ...
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bioRxiv - Bioengineering 2021Quote: 1-Palmitylthio-2-palmitoylamido-1,2-dideoxy-sn-glycero-3-phosphorylcholine (thioetheramide-PC) was purchased from Cayman Chemical (Ann Arbor, Michigan). Hydrogenated soy phosphatidylcholine (HSPC) ...
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bioRxiv - Cancer Biology 2022Quote: ... using a glutathione assay kit based on Ellman’s reagent (DTNB, 5,5′-dithio-bis-2-nitrobenzoic acid) according to the manufacturer’s protocol (Cayman Chemical, t#703002). Data are presented as DNTB fluorescence normalized to total protein ...
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bioRxiv - Cell Biology 2020Quote: ... PLD assay reagent amplex red 10-Acetyl-3,7-dihydroxyphenoxazine and 2-dioctanoyl-sn-glycero-3-phosphocholine (C8-PC) were purchased from Cayman Chemical. Horseradish peroxidase and choline oxidase were purchased from VWR ...
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Activation of innate immune cGAS-STING pathway contributes to Alzheimer’s pathogenesis in 5×FAD micebioRxiv - Neuroscience 2022Quote: Level of 2′3′-cGAMP within neural cell lysates after oligomeric Aβ42 treatment was measured by 2′3′-cGAMP competitive ELISA kit (501700, Cayman Chemical) accordingly to the manufacture’s instruction.
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bioRxiv - Developmental Biology 2022Quote: ... cohorts were pre-treated for 2 hours in mTOR inhibitor (Torin, final concentration 100 nM in fish water, Cayman Chemical). For experiments using rapamycin (LC Labs ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cells were then transferred to a fresh 96 well plate and diluted to 1.0 × 106 cells per ml in hTCM+IL-2 and 0.05 µM of the small molecule inhibitor TSA (Cayman Chemical). The 96 well plate was then spun at 200xg for 7 min and incubated in a tissue culture incubator overnight ...
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bioRxiv - Physiology 2024Quote: ... The uptake of fluorescent 2-NBDG was assessed according to manufacturer’s instructions (Cayman Chemicals - Glucose Uptake Cell-Based Assay Kit) on a Synergy HTX plate reader (BioTek).
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bioRxiv - Plant Biology 2023Quote: ... Approximate 80 μg of total protein was used for biotin labeling in the presence of 0.4 mM thiol-reactive N-[6-(Biotinamido) hexyl]-3’-(2’-pyridyldithio) propionamide (biotin-HPDP; Cayman Chemical). The sample was then incubated for 60 min at 25°C with mild shaking in the dark followed by acetone precipitation and cleanup ...
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bioRxiv - Immunology 2024Quote: ... while the remaining cells were subjected to the same regimen with 2 µM vemurafenib and an additional 1.5 nM cobimetinib (GDC-0973, Cayman Chemical) until a final concentration of 200 nM cobimetinib was attained ...
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bioRxiv - Biochemistry 2024Quote: ... and 15 μL of 10X SARS-CoV-2 Main Protease (recombinant) solution (Cayman Chemical, Item No. 701963, Batch 0673677-1) were added in triplicate ...
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bioRxiv - Biochemistry 2024Quote: ... and 1-stearoyl-2-hydroxy-sn-glycero-3-phosphocholine (18:0 LPC) were purchased from Cayman Chemical (Ann Arbor, MI) and dissolved in ethanol ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated overnight at 4°C in 2% normal goat serum together with mouse anti-nitrotyrosine (1:150; Cayman Chemical). Slides were then washed and incubated in goat anti-mouse biotin secondary antibody (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... followed by overnight incubation at 4°C with the following primary antibodies prepared in antibody diluent (TBS solution with 0.02% Triton X-100 plus 1% bovine serum albumin and 2% normal goat serum): mouse anti-adropin (Cat. No. 14117, 1:200; Cayman Chemical), rat anti-CD31 (Cat ...
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bioRxiv - Pathology 2021Quote: ... cells media was changed to DMEM containing 2% charcoal striped and dialyzed media with 0.1 uM of T0901317 and/or 1uM of LG100268 (noted LG268) (Cayman Chemical, USA). After overnight treatment ...
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bioRxiv - Biochemistry 2021Quote: ... media was replaced with DMEM containing 2% FBS and labelled with 30 μM 17-ODYA (17-Octadecynoic Acid,34450, Cayman Chemicals) or DMSO for 3hr at 37°C incubator ...
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bioRxiv - Cancer Biology 2020Quote: ... inhibitors were pre-incubated with MB solution 2 h before irradiation: Fer-1 (Cayman Chemical, Ann Arbor, Michigan, EUA, 1 µM); Nec-1 (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... and seeded at a density of 500,000 cells per well of a matrigel-coated 6-well plate in iPS-Brew XF supplemented with 2 uM Thiazovivin ROCK inhibitor (Cayman Chemical). Definitive endoderm was derived using STEMdiff™ Definitive Endoderm Kit (STEMCELL Technologies ...
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bioRxiv - Cancer Biology 2022Quote: ... Etomoxir and the KAT2A specific inhibitor cyclopentylidene-[4-(4’-chlorophenyl)thiazol-2-yl)hydrazone (CPTH2)79 were purchased from Cayman Chemical, dissolved in ethanol and used at concentrations of 50 and 2 μM ...
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bioRxiv - Synthetic Biology 2023Quote: Thin films were prepared by dissolving 1mM 1-palmitoyl-2-oleoyl-sn-glycero-3-PC (POPC) and 1 mM cholesterol (Catalog No. 15102, Catalog No. 9003100, Cayman Chemical) in chloroform or dichloromethane in glass vials ...
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bioRxiv - Immunology 2024Quote: ... Cells were then washed in PBS and resuspended at 1×106 cells/mL in fresh media and pre-treated for 2 hours with 0.5mM Glutathione ethyl ester (Et-GSH, Cayman Chemical, 14953) or 50nM UAMC-3203 (Cayman Chemical ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Leukotriene D4 (LTD4, Cat# 20310) and 2-Methylthioadenosine diphosphate (2MeS-ADP, Cat# 21230) were purchased from Cayman Chemical (Ann Arbor, Michigan). HAMI 3379 was purchased from MedChemExpress (Monmouth Junction ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were trypsinized and plated at 40% confluence on fibronectin-coated cover glass in regular GM containing 1μM ERK1/2 inhibitor (Cayman Chemicals; SCH772984). Subsequently ...
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bioRxiv - Molecular Biology 2020Quote: ... Suspensions were centrifuge at 3,000 g for 5 min and the cAMP levels in supernatants were measured using the cAMP EIA Kit (Cayman Chemical, Ann Arbor, MI, US) according to the manufacturer’s instructions ...
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Ventral tegmental area glutamate neurons mediate the nonassociative consequences of traumatic stressbioRxiv - Neuroscience 2021Quote: ... Baseline firing in response to current injections was collected for 5 min followed by a 10 min bath application of 10 μM clozapine (Cayman Chemical, Ann Arbor, MI). The average number of action potentials generated during five consecutive sweeps was used to quantify baseline activity and the aver-age of five consecutive sweeps during clozapine application was used to quantify firing during hM4Di receptor activation ...
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bioRxiv - Biophysics 2022Quote: ... were induced along the PM of cultured HeLa cells by adding 10-15% ethanol (25) or 5 µM cytochalasin D (Cayman Chemical, Ann Arbor, MI) to the cell growth medium and incubating for ∼15 min at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2021Quote: ... neurons were washed for 3 h and incubated for 30 min (37 °C, 5% CO2) with either 25 µM spermine-NONOate (a nitric oxide donor; Cayman Chemical, cat. no. 82150) or 25 µM sulpho-NONOate (control compound ...
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bioRxiv - Neuroscience 2023Quote: ... Mice were then injected 30 minutes prior to behavioral experiments intraperitoneally with 5 mg/kg of the iDREADD agonist clozapine-n-oxide (CNO; Cayman Chemical, #34233-69-7) and returned to their homecage until behavioral testing ...
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bioRxiv - Neuroscience 2024Quote: ... C18:2-d4, C20:4-d11, C20:5-d5, C22:0-d43, C22:6-d5, C24:0-d4; 10 ng each; Cayman Chemical, Ann Arbor MI). HPLC measurements were performed using an Agilent 1290 HPLC system with binary pump ...
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bioRxiv - Developmental Biology 2022Quote: ... (gift from Jeremy Nathans (41)) or C3H10T1/2-STF (ATCC CCL-226) cells were treated with 1 µM LGK-974 (Cayman Chemical 14072) for 48 h to inhibit endogenous WNT secretion ...
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bioRxiv - Microbiology 2020Quote: Neutralizing assay was performed using SARS-CoV-2 Neutralizing Antibody Detection ELISA Kit (Cayman Chemical, Ann Arbor, MI, USA; Item No. 502070) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2022Quote: ... were immobilized in a PDMS microfluidic olfactory chip [58,59] and exposed to either a control buffer or a stimulus buffer containing 100mM 2-AG (Cayman Chemical Co. #62160). The required volume of 2-AG required to make 100mM solution was dissolved in 0.1% ethanol before being added to S-Basal buffer ...
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bioRxiv - Physiology 2023Quote: ... The reaction proceeded for 2 hours at 37°C in presence of either 300 nM MS-275 (Cayman Chemical, Ann Arbor, MI) or vehicle (0.01% DMSO) ...