Labshake search
Citations for Alfa Aesar :
1 - 36 of 36 citations for Lysozyme C LYZ Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: For treatment with lysozyme (1 mg/mL, Alfa Aesar) or proteinase K (Roche) ...
-
bioRxiv - Microbiology 2019Quote: ... the cell pellet obtained from initial centrifugation was incubated at 37°C for 1 hour with 300 rpm mixing in 250 μL 3 mg/mL lysozyme (Alfa Aesar, Haverhill, MA) in Tris-EDTA buffer (Sigma-Aldrich ...
-
bioRxiv - Synthetic Biology 2021Quote: ... in-gel using Novex Colloidal Coomassie stain against a hen egg white lysozyme (HEWL; Alfa Aesar) standard ...
-
bioRxiv - Microbiology 2021Quote: ... Pellets were resuspended in 800 µL TE buffer (10 mM Tris pH 8.0, 1 mM EDTA) + 15 mg/mL lysozyme (Alfa Aesar J60701-06) and incubated at 37°C for 16 hours ...
-
bioRxiv - Microbiology 2022Quote: ... Upon thawing, pellets were resuspended in 800 µl TE buffer (10mM Tris pH 8.0, 1mM EDTA) + 15mg/mL lysozyme (Alfa Aesar J60701-06) and incubated at 37 °C for 16h ...
-
bioRxiv - Microbiology 2023Quote: ... Upon thawing, pellets were resuspended in 800 µl TE buffer (10mM Tris pH 8.0, 1mM EDTA) + 15mg/mL lysozyme (Alfa Aesar J60701-06) and incubated at 37 °C for 16h ...
-
bioRxiv - Neuroscience 2024Quote: ... (C) (+)-Citronellal (∼96% Alfa Aesar), (D ...
-
bioRxiv - Cancer Biology 2019Quote: ... human DiI-VLDLs (1 mg protein/mL; Alfa Aesar Chemicals), LPL from bovine milk (Sigma-Aldrich ...
-
bioRxiv - Bioengineering 2019Quote: ... either human recombinant myelin basic protein (Alfa Aesar, cat. #AAJ66051LB0) fluorescently labeled with Vybrant DiD (Molecular Probes ...
-
bioRxiv - Cell Biology 2023Quote: ... Mitomycin C was purchased from Alfa Aesar (Cat # J63193). Carmustine was purchased from MedChemExpres (Cat # HY-13585/CS-2935) ...
-
bioRxiv - Immunology 2020Quote: ... for 4 hours at 37 °C in TBS (Alfa Aesar) buffer containing 2 mM CaCl2 ...
-
bioRxiv - Biochemistry 2019Quote: ... Acetylated LDL derived from human plasma was purchased from Alfa Aesar (Haverhill, MA) RNEasy Mini kit was purchased from Qiagen (Hilden ...
-
bioRxiv - Developmental Biology 2022Quote: ... the cells were incubated with 10 µg/mL mitomycin C (J63193, Alfa Aesar) in culture media at 37 □ for two hours ...
-
bioRxiv - Genomics 2022Quote: ... Cells were treated with DNA intercalating agent Mitomycin C (MMC, Alfa Aesar J63193MA) reconstituted in DMSO at a final concentration of 10ug/ml ...
-
bioRxiv - Microbiology 2022Quote: ... 1-mL aliquots of de-lipidated human serum (J65516, BT-931, Alfa Aesar, Haverhill, MA, USA) were stored at −20 °C and thawed only once prior to use ...
-
bioRxiv - Cancer Biology 2020Quote: ... Residual red blood cells were removed using RBC Lysis Buffer for Human (Alfa Aesar, cat. # J62990) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: ... Samples were heated to 95°C for 5 minutes in Laemmli buffer (Alfa Aesar), separated on a Novex 4-20% Tris-glycine gel (ThermoFisher Scientific ...
-
bioRxiv - Cell Biology 2022Quote: ... then replaced immediately with 37°C-prewarmed cytoskeleton buffer containing 4% paraformaldehyde (Alfa Aesar 43368) and fixed cells for 15 min ...
-
bioRxiv - Microbiology 2020Quote: ... the DNA was resuspended in 100 μL of warmed (65 C) Elution Buffer (Alfa Aesar, Massachusetts) and stored at −20 C.
-
bioRxiv - Cell Biology 2021Quote: ... cells were incubated at 37°C for 30 minutes in 5 μM propidium iodide (Alfa Aesar) and 1:1500 Hoechst 33342 (Molecular Probes ...
-
bioRxiv - Neuroscience 2020Quote: ... and then fixed overnight at 4°C in 4% paraformaldehyde (Alfa Aesar, Thermo Fisher Scientific, UK), followed by several washes in 0.1M Phosphate buffered saline (PBS ...
-
bioRxiv - Cell Biology 2023Quote: ... and then hybridized overnight at 37°C in hybridization buffer (10% formamide, 10% dextran sulphate (J62787.18, Alfa Aesar), 250nM smFISH probe(s) ...
-
bioRxiv - Cell Biology 2023Quote: ... at 37°C for the indicated durations at the following concentrations: bafilomycin A1 (BafA1; 0.1 µg/mL; Alfa Aesar), carbonyl cyanide m-chlorophenyl hydrazine (CCCP ...
-
bioRxiv - Cancer Biology 2019Quote: ... and cryopreserved at 10-15×106 cells/cryovial (RPMI-1640, Corning #15-040-CV; 20% human AB serum, Valley Biomedical #HP1022; 10% DMSO, Alfa Aesar #42780).
-
bioRxiv - Biochemistry 2021Quote: ... Bound proteins (‘output’) were eluted by boiling the beads (95 °C, 10 minutes) with 1x Laemelli sample buffer (Alfa Aesar) containing 30 mM DTT ...
-
bioRxiv - Biochemistry 2021Quote: ... Bound proteins (‘output’) were eluted by boiling the beads for 10 min at 95 °C with 1.5 × Laemelli sample buffer (Alfa Aesar) containing 30 mM DTT ...
-
bioRxiv - Cell Biology 2021Quote: ... washed twice with phosphate buffered saline (PBS) at 37°C and fixed with 4% paraformaldehyde (PFA) in PBS (Alfa Aesar) at 37°C ...
-
bioRxiv - Immunology 2020Quote: ... BL21 cells were spun down (3000 RPM, 30 min, 4 °C) and resuspended in Tris-buffered saline (TBS, Alfa Aesar) containing cOmplete™ protease inhibitor cocktail (Sigma Millipore) ...
-
bioRxiv - Biochemistry 2019Quote: ... UK) for 2 mins at 37°C prior to the addition of calcium chloride (25 μl, 50 mM; Alfa Aesar, UK). The time taken for clot formations to occur (an upper maximal of 2 mins was imposed ...
-
bioRxiv - Synthetic Biology 2021Quote: ... The supernatants were collected by centrifugation at 3,200 x g at 4°C for 30 min and the pH was adjusted to the same value as fresh media 5N KOH (Alfa Aesar) with the Mettler Toledo InLab Micro pH electrode ...
-
bioRxiv - Cell Biology 2021Quote: Yeast cultures were grown to an OD600 of 0.4-0.6 at 30°C and then pre-treated with 100 mM hydroxyurea (Alfa Aesar, Tewksbury, MA) for 2 hours at 30°C ...
-
bioRxiv - Bioengineering 2022Quote: ... The supernatant was transferred to a new bottle and stored at 4 °C until use. N,N’-methylenebis(acrylamide) (BIS; catalog no. J66710) was purchased from Alfa Aesar. Ammonium persulfate (APS ...
-
bioRxiv - Biochemistry 2023Quote: ... Samples were then boiled for 8 min at 90 °C after addition of 4×reducing Laemmli SDS sample loading buffer (Alfa Aesar) and ran on precast 4−20% Criterion TGX gels (Bio-Rad).
-
bioRxiv - Synthetic Biology 2023Quote: ... dendrorhous type strain CBS 6938 (ATCC 96594) was cultured at 21 °C in YPD (YEP, Sunrise Science, 1877-1KG plus 20 g/L glucose, Alfa Aesar, A16828) media for all experiments ...
-
bioRxiv - Microbiology 2021Quote: ... all dishes were incubated at 37°C with 5% CO2 before being washed with sterile PBS and fixed with 4% paraformaldehyde (Alfa Aesar, Tewksbury, MA). Confocal laser scanning microscopy (CLSM ...
-
bioRxiv - Biochemistry 2021Quote: ... LD membrane proteins and control proteins were synthesised in parallel either in the presence or absence of ER-derived dog pancreatic microsomes at 30°C for 7 min and further translation initiation was blocked with 0.1 mM aurintricarboxylic acid (Alfa Aesar, cat. number A15905). Samples were incubated at 30°C for a further 8 min ...