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Citations for Alfa Aesar :
251 - 278 of 278 citations for tert Butyl 10 oxo 4 9 diazaspiro 4.5 decane 4 carboxylate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... for 10 min at room temperature and the slides mounted with a 90% glycerol (Alfa Aesar), 0.5% (w/v ...
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bioRxiv - Cell Biology 2023Quote: Differentiated BMECs at day 10 were analysed with the Dil-ac-LDL dye (J65597, Alfa Aesar). Culture medium was aspirated then incubated in medium containing 12 μg/mL Dil-ac-LDL for 4 h at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were then diluted with 10 µL of 4x reducing Laemmli SDS sample loading buffer (Alfa Aesar), boiled at 95° C for 5 min ...
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bioRxiv - Biochemistry 2020Quote: ... Cryoprotection was achieved by washing in reservoir solution containing 10% of (R,R)-2,3-butandiol (Alfa Aesar) for a few minutes prior to flash-cooling in liquid nitrogen.
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bioRxiv - Microbiology 2023Quote: ... 10 mM NaNO3 (195-02545; FUJIFILM Wako, Japan) and 0.01% L-tryptophan (A10230; Alfa Aesar, United States) was prepared as a general incubation medium ...
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bioRxiv - Cell Biology 2023Quote: ... cells were fixed for 10 minutes with room temperature (RT) 3.5% PFA (paraformaldehyde, pH 6.7; Alfa Aesar). Cells were then washed 3 x with Tris buffered saline (TBS ...
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bioRxiv - Immunology 2022Quote: ... RNA (stored in RNAse-free water) was diluted in 10 mM citrate buffer at pH 3.0 (Alfa Aesar). The lipids and RNA were mixed using the NanoAssemblr Ignite instrument (Precision Nanosystems ...
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bioRxiv - Cancer Biology 2020Quote: ... 20 μL of sample or standard was taken and 10 μL of internal standard (valeric acid, Alfa Aesar, UK) added prior to the addition of 5 μL 15% percholoric acid ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were incubated with 10 μM of the blocking agent 7-bromoheptanol (Merrill et al. 2019) (Alfa Aesar, H54762), for 2 h ...
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bioRxiv - Microbiology 2023Quote: ... The strain was maintained on yeast extract-peptone-dextrose (YPD) solid medium (10 g/l yeast extract [Alfa Aesar, United States of America] ...
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bioRxiv - Biochemistry 2021Quote: ... Bound proteins (‘output’) were eluted by boiling the beads (95 °C, 10 minutes) with 1x Laemelli sample buffer (Alfa Aesar) containing 30 mM DTT ...
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bioRxiv - Biochemistry 2021Quote: ... Bound proteins (‘output’) were eluted by boiling the beads for 10 min at 95 °C with 1.5 × Laemelli sample buffer (Alfa Aesar) containing 30 mM DTT ...
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bioRxiv - Microbiology 2021Quote: ... Bacterial strains carrying plasmids were maintained using the corresponding antibiotics at the indicated concentrations: 10 µg mL−1 of thiamphenicol (Tm; Alfa Aesar), 50 µg mL−1 of kanamycin (Kan ...
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bioRxiv - Microbiology 2020Quote: ... were impregnated with 5 μl of a freshly made stock antibiotic solution of either 40 mg/ml streptomycin sulfate (MilliporeSigma) in sterile distilled water or 10 mg/ml oxytetracycline hydrochloride (Alfa Aesar) in sterile distilled water to reach a concentration of 200 μg for streptomycin and 50 μg for oxytetracycline in each disc ...
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bioRxiv - Bioengineering 2021Quote: EPCs were cultured in serum-free medium for 12 h and then incubated with 10 µg/ml Dil-Ac-LDL (Alfa Aesar) for 5 h at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... Non-specific antigens were blocked with 10% normal goat serum in TBS containing 0.3% Triton X-100 (Alfa Aesar, Haverhill, MA). Slides were then incubated with the respective primary antibody (Supplemental Table S2 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were pelleted at 200g for 5 minutes and re-suspended in serum free RPMI media and cross-linked for 10 minutes on a rocker by adding 16% methanol-free paraformaldehyde (Alfa Aesar, 43368) (final concentration 1%) ...
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bioRxiv - Microbiology 2021Quote: ... Pellets were resuspended in 800 µL TE buffer (10 mM Tris pH 8.0, 1 mM EDTA) + 15 mg/mL lysozyme (Alfa Aesar J60701-06) and incubated at 37°C for 16 hours ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were lysed in the digitonin lysis buffer (1% digitonin [Sigma-Aldrich D141], 137 mM NaCl, 10% w/v glycerol [Alfa Aesar 38988] ...
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bioRxiv - Microbiology 2022Quote: ... the effect of viscosity on synovial fluid-induced aggregation was assessed by imaging 1-hour after incubation in 10% BSF supplemented with increasing concentrations of high molecular weight hyaluronic acid sodium salt (1.5-1.8MDa) (Alfa Aesar, Haverhill, MA, USA) in PBS ...
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bioRxiv - Microbiology 2022Quote: ... with additions of carbohydrate and NH4Cl (25 and 10 mM, respectively) or purines (uric acid, Sigma-Aldrich cat. # U0881, 1 mg/ml; adenine, Alfa Aesar cat ...
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bioRxiv - Neuroscience 2022Quote: ... were pulled to a shank length of approximately 0.6 cm (5-8 MΩ) using a Narishige PC-10 puller (Tokyo, Japan) and filled with 2% Pontamine sky blue dye (Alfa Aesar, Haverhill, MA) dissolved in sodium acetate (0.5 M ...
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bioRxiv - Neuroscience 2024Quote: ... dissected retinas were flash frozen at −80°C for 10 minutes and then lysed in 100 µL of RIPA buffer (Alfa Aesar, Cat# J63306) + protease inhibitor cocktail (GenDepot Cat# P3100-001) ...
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bioRxiv - Biochemistry 2020Quote: ... two sequential 24-hour pre-cultures were grown on 1 % ethanol minimal medium (EMM) media containing 15 and 10 nM β-estradiol (Alfa Aesar, Th. Geyer), respectively ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were lysed at RT for 10 min using freshly prepared lysis buffer: RIPA buffer with EDTA and EGTA (Alfa Aesar/Thermo Fisher Scientific) + 5% SDS + 2x cOmplete protease inhibitor cocktail (Roche) ...
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bioRxiv - Bioengineering 2021Quote: ... VHHs were enzymatically biotinylated in their BAP tag by incubating 1 mg of purified VHHs with 10 µM of ATP (Alfa Aesar, Haverhill, MA, Cat#CAAAJ61125-09), 100 µM of D-(+)-biotin (VWR ...
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bioRxiv - Microbiology 2022Quote: ... Biotin was conjugated to the probes in a click reaction with 0.4 mg cleared lysate in 180 μL PBS and 20 μL 10X click master mix (1 mM azido-PEG3-biotin [Alfa Aesar], 10 mM tris(2-carboxyethyl)phosphine hydrochloride ...
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bioRxiv - Microbiology 2021Quote: ... cells were transferred from rich YPD medium (20 g/L Bacto Peptone from BD, 10 g/L yeast extract from Alfa Aesar, 20 g/L glucose from Fisher) to the defined YSM medium (1.7□g/L yeast nitrogen base without amino acids and without ammonium sulfate from BD Difco ...