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1 - 10 of 10 citations for Cyclosporin A 13C2 d4 Acetate since 2020

• Vaginal Lactobacillus fatty acid response mechanisms reveal a novel strategy for bacterial vaginosis treatment

  Meilin Zhu, et al., bioRxiv - Microbiology 2023

  Quote: ... thymine-d4 and glycocholate-d4 internal standards (Cambridge Isotope Laboratories. The cell pellets were homogenized using the QIAGEN TissueLyser II with 3mm Tungsten beads for 4 min at a frequency of 20 Hz ...
• ENDOTHELIAL GENE REGULATORY ELEMENTS ASSOCIATED WITH CARDIOPHARYNGEAL LINEAGE DIFFERENTIATION

  Ilaria Aurigemma, et al., bioRxiv - Molecular Biology 2023

  Quote: Total RNA was isolated from d2 (n. 2 biological replicates) and d4 (n. 2 biological replicates) cells with QIAzol lysis reagent (Qiagen #79306), according to the manufacturer’s protocol ...
• A spontaneous genetically-induced epiallele at a retrotransposon shapes host genome function

  Tessa M. Bertozzi, et al., bioRxiv - Genetics 2020

  Quote: ... denaturation solution (0.2M NaOH), and wash buffer (10 mM Tris-acetate, pH 7.6) using the PyroMark Q96 Vacuum Workstation (QIAGEN). Purified DNA resuspended in annealing buffer (20 mM Tris-acetate pH7.6 ...
• A nanobody suite for yeast scaffold nucleoporins provides details of the Nuclear Pore Complex structure

  Sarah A Nordeen, et al., bioRxiv - Biochemistry 2020

  Quote: Initial hits of Nup851-564-Seh1-VHH-SAN2 were obtained at 18 °C in 1 day in a 96-well sitting drop tray with a reservoir containing 1M ammonium sulfate and 0.1 M sodium acetate pH 5.0 (Protein Complex suite, Qiagen). Crystals significantly improved by changing the buffer to di-sodium succinate pH 5.5 and the addition of 4% (v/v ...
• The 3-phosphoinositide-dependent protein kinase 1 is an essential upstream activator of protein kinase A in malaria parasites

  Eva Hitz, et al., bioRxiv - Microbiology 2021

  Quote: ... Protein lysates were separated on NuPage 5-12% Bis-Tris or 3-8% Tris-Acetate gels (Novex, Qiagen) using MES running buffer (Novex ...
• Mechanisms for Electron Uptake by Methanosarcina acetivorans During Direct Interspecies Electron Transfer

  Dawn E. Holmes, et al., bioRxiv - Microbiology 2021

  Quote: ... Total RNA was extracted from cell pellets as previously described (61), and all six RNA samples (3 acetate, 3 DIET) were cleaned with the RNeasy Mini Kit (Qiagen) and treated with Turbo DNA-free DNase (Ambion) ...
• Nucleic acid sequence contributes to remodeler-mediated targeting of histone H2A.Z

  Cynthia Converso, et al., bioRxiv - Molecular Biology 2023

  Quote: ... the FT and Eluate fractions (90 µL each) were mixed with 10 µL 3 M sodium acetate and applied to a QIAquick spin column (Qiagen). Purified DNA was visualized a 1.3% agarose / 0.5x TBE gel and SYBR Green staining ...
• Membrane Bound O-Acyltransferase 7 (MBOAT7) Shapes Lysosomal Lipid Homeostasis and Function to Control Alcohol-Associated Liver Injury

  Venkateshwari Varadharajan, et al., bioRxiv - Pathology 2024

  Quote: ... liver was homogenized in 500uL of 3:1:6 isopropanol:water:ethyl acetate containing internal standard in ceramic bead tubes (Qiagen #13113-50) using the TissueLyzer II (Qiagen #9244420) ...
• Purified diets containing high levels of soluble fiber and grain-based diets promote similar gastrointestinal morphometry yet distinct microbial communities

  Elaine M. Glenny, et al., bioRxiv - Microbiology 2024

  Quote: ... DNA was precipitated with 100% ethanol and 3 M sodium acetate at -80°C for 60 minutes and then cleaned using the Qiagen QIAamp DNA Stool Mini Kit (Qiagen, Valencia, CA) per manufacturer’s instructions.
• Comprehensive mutagenesis maps the effect of all single codon mutations in the AAV2 rep gene on AAV production

  Nina K. Jain, Pierce J. Ogden, George M. Church, bioRxiv - Synthetic Biology 2023

  Quote: ... we ran the digest products on 1% Tris-acetate EDTA gels and extracted and purified the correctly sized products (Qiagen QIAQuick Gel Extraction Kit). In parallel ...