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Citations for Qiagen :
1 - 48 of 48 citations for CRISPR Screening since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The initial screening was performed using commercially available screening kits from Qiagen and Molecular Dimensions in 96-well plate format using sitting-drop technique ...
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bioRxiv - Biochemistry 2020Quote: ... screening PACT Suite and JCSG Suite (Qiagen), JBScreen Classic 1-4 and 6 (Jena Bioscience ...
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bioRxiv - Microbiology 2023Quote: ... screening PACT Suite and JCSG Suite (Qiagen), JBScreen Classic 1-4 and 6 (Jena Bioscience ...
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bioRxiv - Microbiology 2021Quote: ... for screenings or exoEasy Maxi Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocols.
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bioRxiv - Biochemistry 2023Quote: ... commercial screening solutions (Emerald Biosystems, Hampton Research, and Qiagen), and the sitting-drop vapor-diffusion technique (drop ...
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bioRxiv - Developmental Biology 2024Quote: ... run by QIAxcel DNA Screening Kit (QIAGEN, Cat. No. 929004), detected by High Performance Nucleic Acid Analyzer (eGENE HDA-GT12 ...
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bioRxiv - Zoology 2020Quote: ... The PCR products were analyzed with the QIAxcel DNA Screening Kit (Qiagen) to confirm successful amplification ...
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bioRxiv - Biochemistry 2020Quote: ... Crystallization screening was performed at 4°C with JCSG core I (Qiagen) using the sitting drop vapor diffusion method ...
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bioRxiv - Biochemistry 2020Quote: ... 14 and 22 mg/mL for initial screening using commercially available screens (Qiagen) by sitting-drop vapor diffusion using a Crystal Gryphon robot (Art Robbins Instruments) ...
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bioRxiv - Biochemistry 2023Quote: ... 25 %(w/v) PEG 2000 MME from PEGs I suite screening plate (Qiagen). Then ...
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bioRxiv - Biochemistry 2020Quote: Purified CrTRXz was tested for crystallization on commercial sparse-screening conditions (Qiagen, Hilden Germany) of the Joint Center for Structural Genomics screens [97] with a mixture of 30 nL protein and 30 nL precipitant equilibrated against a reservoir volume of 30 µL mother liquor ...
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bioRxiv - Bioengineering 2020Quote: ... Crystallization screening was performed in multiple 96-condition spare matrix suites available from Qiagen or Hampton Research ...
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bioRxiv - Microbiology 2022Quote: ... and amplicons were separated in QIAxcell Advanced using the QX DNA Screening Kit (Qiagen).
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bioRxiv - Microbiology 2022Quote: ... and amplicons were separated in QIAxcell Advanced using the QX DNA Screening Kit (Qiagen). The primers used for the MLST study are listed on Supplementary Table S2 ...
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GFI1 cooperates with IKAROS/IKZF1 to activate gene expression in T-cell acute lymphoblastic leukemiabioRxiv - Biochemistry 2021Quote: ... and with IKAROS or control CRISPR RNP knockout using the RNeasy Mini kit (Qiagen) and RNase- Free DNase Set (Qiagen) ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was isolated from depleted cells and CRISPR clones using RNAeasy kit (Qiagen) and treated with TURBO DNA-free™ DNase I kit (Invitrogen Ambion ...
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bioRxiv - Immunology 2024Quote: ... DNA was extracted from CRISPR edited cells using DNsasy Blood & Tissue Kit (QIAGEN, 69506). DNA samples were PCR amplified using KOD Hot Start DNA Polymerase (MilliporeSigma ...
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bioRxiv - Biochemistry 2021Quote: ... Initial conditions were identified in 96-well sparse matrix screening trays (Qiagen and Hampton Research) dispensed by an Art Robbins Instruments Phoenix liquid-handling robot ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR products were visualised using a QIAxcel DNA screening kit in a QIAxcel instrument (Qiagen).
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bioRxiv - Cell Biology 2024Quote: ... The autophagy screening was performed using the RT2 Profiler™ PCR Array Human Autophagy (Qiagen) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: Genomic DNA from CRISPR-edited U937 clones was isolated with a DNeasy kit (Qiagen 69506) per manufacturer instructions ...
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bioRxiv - Microbiology 2019Quote: ... each CRISPR-Cas island was inspected and confirmed using CLC Genomics Workbench version 9.5.3 (QIAGEN) for the purpose of verifying the conservation of the Cas operon architecture.
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bioRxiv - Plant Biology 2020Quote: ... Total RNA from CRISPR/Cas9 mutants was extracted with RNeasy® Plant Mini Kit (Qiagen, US) following manufacturer’s instruction ...
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bioRxiv - Biochemistry 2019Quote: Screening of crystallization conditions was performed using a Mosquito drop dispensing automate (TTPLabtech) and crystallization screens (Qiagen). Drops were prepared by mixing 150 nL of protein solution with an equivalent volume of screening solution ...
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bioRxiv - Cell Biology 2021Quote: ... CRISPR/Cas9 and donor plasmids were co-transfected into the cell lines using Effectene (Qiagen, Venlo, Netherlands). For drug selection ...
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bioRxiv - Cancer Biology 2022Quote: ... genomic DNA was extracted from cell pellets from CRISPR or base editing screens (DNeasy Blood & Tissue; Qiagen), gRNA DNA sequences were PCR amplified (empirically determined number of cycles ...
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bioRxiv - Microbiology 2022Quote: Twenty-four multi-amplicon libraries were prepared using the QIAseq 16S/ITS Screening Panel kit (Qiagen, Hilden, Germany) as detailed below ...
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bioRxiv - Biochemistry 2023Quote: ... Screening plates were set up by a sitting-drop vapor diffusion method using JCSG Core I-IV (Qiagen), PEG Suite I-II (Qiagen) ...
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bioRxiv - Molecular Biology 2019Quote: Preliminary screening for the presence of serum exosomal miRNAs was determined using a miScript human miFinder PCR array (MIHS-001Z, Qiagen). RNA was converted to cDNA using miScript RT kit (218060) ...
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bioRxiv - Systems Biology 2021Quote: ... Crystal screening was performed with a Gryphon robot (Art Robbins instruments, Sunnyvale, USA) using sitting-drop 96-well plates and Screening Suites (Qiagen). Optimization experiments were set up in 24-well hanging drop crystallization plates ...
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bioRxiv - Microbiology 2020Quote: ... The Veriti 96-Well Thermal Cycler was used and amplicons were separated in the QIAxcell Advanced using QX DNA Screening Kit (Qiagen).
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bioRxiv - Genomics 2023Quote: Total DNA from enhancer screening library transduced HUDEP-2 cells was isolated using the DNeasy Blood & Tissue kit (Qiagen, 69504) and the enhancer inserts were PCR amplified using the following primers:
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bioRxiv - Biochemistry 2024Quote: ... Initial screening was conducted using the sitting-drop vapor diffusion method with the ARI Crystal Gryphon Robot (ARI) and crystallization screening kits (QIAGEN) at 18°C ...
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bioRxiv - Genomics 2020Quote: ... and a library representation of >200 colonies per guide was determined prior to CRISPR library pool plasmid purification by Endofree Maxi (Qiagen). Coverage of the 818 guide library for Perturb-CITE-Seq as well as the extended control library was confirmed by PCR amplification of the guide sequence from the final library pool and deep sequencing on an Illumina MiSeq.
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bioRxiv - Molecular Biology 2020Quote: Genomic DNA from CRISPR-on HEK293T cells and control cells were purified using a AllPrep DNA/RNA/Protein Mini Kit (Qiagen). Then ...
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bioRxiv - Genetics 2022Quote: ... and larvae (12 CRISPR-treated, 20 water-injected and 13 uninjected) selected for sequencing by using DNeasy Blood & Tissue Kit (QIAGEN) Purity and concentration of DNA samples were measured with a NanoDrop spectrophotometer (Thermo Fisher Scientific).
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A CRISPR activation screen identifies FBXO22 as an E3 ligase supporting targeted protein degradationbioRxiv - Biochemistry 2023Quote: HEK293T CRISPR transcriptional activation cells after sgRNA transduction were collected and total RNAs were extracted using the RNeasy mini kit (Qiagen). cDNAs were obtained by reverse transcription using the iScript cDNA kits (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted from cell lines in 3 biological replicates after each CRISPR/Cas9 oncogene downregulation using QIAzol (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: Purified CrFBA3 was tested for crystallization at two concentration of 9 mg/ml and 4.5 mg/ml on commercial sparse-screening conditions (Qiagen, Hilden Germany) based on the work of Jancarik and Kim (Jancarik ...
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bioRxiv - Immunology 2020Quote: Initial crystallization hits were obtained via sparse matrix screening in 96-well plates using commercially available crystallographic solutions (Qiagen, Toronto, ON). Optimization of crystallization conditions was performed in 24-well plate format using the hanging drop vapor diffusion method ...
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bioRxiv - Cell Biology 2021Quote: ... The plasmid DNA was extracted from 3-9 positive colonies where possible after blue-white screening using a QIAprep® Spin Miniprep Kit (Qiagen) and sequenced using the standard T7 primer at Eurofins Genomics ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmid sgRNA CRISPR porthos was co-transfected (1 µg of total DNA per well) with Effectene Tranfection Reagent (Qiagen, Hilden, Germany) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... The cell pool was cultured to produce a CRISPR-pool stock and allow genomic DNA extraction (DNeasy kit, Qiagen, Cat#69504). Successful CRISPR editing was visualized by PCR amplification of the target region (primers listed in Table S1) ...
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bioRxiv - Synthetic Biology 2023Quote: The genomic DNA of the CRISPR-Cas3 engineered strains after vector curing was isolated using the DNeasy UltraClean Microbial Kit (Qiagen, Germany) according to the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2023Quote: ... The DDR-CRISPR library plasmid DNA was purified from transformed bacterial culture using a Qiagen EndoFree plasmid DNA Maxi kit (Qiagen, #12362).
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bioRxiv - Genomics 2021Quote: ... Genomic DNA was extracted either from newborn screening bloodspots or infant/child venipuncture samples using the Puregene DNA Extraction Kit (Qiagen, Valencia, California). Input amounts of DNA from infant blood spots were 200-500 ng and inputs from venipuncture samples were 2-3 ug ...
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bioRxiv - Biochemistry 2023Quote: ... the initial crystallization screening was performed using the sitting-drop vapor-diffusion method with Screen classics and Classics II crystallization kits (Qiagen, Hilden, Germany) and PACT kits (Molecular Dimensions ...
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bioRxiv - Biophysics 2023Quote: ... were first obtained using the sitting drop vapor diffusion method using high-throughput crystallization screening kits (Hampton Research, Molecular Dimensions and QIAGEN). Crystals were then grown in a mixed solution containing 1 μl complex solution and 1 μl of reservoir solution using the hanging drop vapor diffusion method at 16°C ...