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Citations for Qiagen :
251 - 300 of 2037 citations for L β Homo β 2 hydroxyphenyl glycine; R 3 Amino 3 2 hydroxyphenyl propanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... brain hemispheres were homogenized in freshly prepared lysis buffer (0.6 ml per 30 mg of brain tissue) containing 1% β-mercaptoethanol using a TissueRuptor homogenizer (Qiagen).
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bioRxiv - Cell Biology 2023Quote: Cells were harvested directly in Buffer RLT with β-mercaptoethanol and RNA processed directly using the RNeasy Mini Kit (Qiagen), with on-column DNase digestion according to manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: ... for 5 minutes and immediately lysed in the β-Mercaptoethanol-containing lysis solution from the RNeasy minikit (Qiagen Cat # 74104). RNA was extracted following the Qiagen kit instruction manual and eluted in 10mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: ... whereas 30 mg of lung tissue was homogenized in 600 μl RLT-buffer containing β–mercaptoethanol for 30 sec at 15 Hz using the TissueLyser II (Qiagen).
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bioRxiv - Molecular Biology 2020Quote: ... level relative to nuclear gene (β actin) by real-time PCR in total DNA isolated from INS1 cells by QIAamp DNA minikit (Qiagen). Rat primers were mt-ND6 ...
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bioRxiv - Immunology 2021Quote: ... 30mg of whole spleen RNA was extract via 30 second homogenization in RLT lysis buffer containing 1% β-mercaptoethanol per RNeasy kit (Qiagen). RNA was reverse transcribed into cDNA using iScript cDNA synthesis kit (BioRad) ...
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bioRxiv - Genomics 2021Quote: HUVEC or FACS-sorted zebrafish ECs were lysed with RLT buffer containing β-mercaptoethanol and RNA was isolated with the RNeasy Plus Mini or Micro Kit (Qiagen), respectively ...
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bioRxiv - Cancer Biology 2022Quote: ... Tissue scrolls were sectioned from OCT-embedded fresh-frozen samples into Buffer RLT with β-mercaptoethanol (Qiagen Sciences LLC, Germantown, MD). RNA was purified following the protocol from RNeasy Micro Kit (Qiagen) ...
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bioRxiv - Immunology 2020Quote: ... harvested and stored at −80°C in RLT buffer with β-mercaptoethanol from the RNeasy Mini prep Kit (Qiagen, Hilden, Germany) according to manufacturer’s instructions until cDNA synthesis for RT2 Profiler PCR array ...
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bioRxiv - Immunology 2022Quote: ... macrophages were lysed with 300μL lysis buffer with 1% v/v β-mercaptoethanol (Themo #12183025) and passaged through a QIAshredder homogenizer column (Qiagen #79656). Total RNA was isolated (Themo #12183025 ...
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bioRxiv - Cell Biology 2020Quote: ... 350μL RLT buffer supplemented with 1% pure β-mercaptoethanol were added and the cell slurry was transferred onto a QIAshredder spin column (Qiagen). Subsequently ...
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bioRxiv - Immunology 2020Quote: ... Pellets were lysed by vortexing for 1 minute in 350uL cold supplemented RLT buffer (RLT + β-MeOH) at 4°C and lysates homogenized using QIAshredder columns (Qiagen). RNA was then extracted from these samples using the RNeasy Plus Micro kit (Qiagen ...
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bioRxiv - Immunology 2020Quote: ... B220+Fas+CD38- CD45.1+ or CD45.1-) were sorted from immunized recipients directly into RLT buffer supplemented with 1% β-ME (Qiagen, Hilden, Germany). mRNA was extracted with an RNeasy Micro Kit (Qiagen ...
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bioRxiv - Pathology 2021Quote: ... Samples were lysed in 175 μL of RNA lysis buffer added with β-mercaptoethanol and 350 μL of RNA dilution buffer for tissue disruption using a TissueLyser II LTTM (Qiagen). Samples were incubated at 70°C for 3 min ...
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bioRxiv - Immunology 2020Quote: ... cells were lysed in RLT lysis buffer containing β-mercaptoethanol and total RNA extracted using the RNeasy micro kit (Qiagen, USA). Total RNA was assessed for quality and quantified using a total RNA 6000 Nano LabChip on a 2100 Bioanalyzer (Agilent Inc. ...
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bioRxiv - Physiology 2020Quote: ... A cross-sectional piece of kidney containing cortex and medulla was homogenized in Buffer RLT with 0.01% β-ME and further homogenized with Qiashredder columns (Qiagen 79654). mRNA concentration was measured by Nanodrop (ThermoFisher) ...
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bioRxiv - Cancer Biology 2022Quote: ... Tissue lysis was performed by re-suspending ∼30mg of sliced frozen tissue in a solution containing 1% β-mercaptoethanol in RLT buffer (supplemented with antifoam agent; ID 19088, Qiagen). Next ...
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bioRxiv - Neuroscience 2024Quote: ... stimulated microglial- and astroglial cells were lysed using β-mercaptoethanol supplied RLT buffer according to the manufacturer’s protocol (Qiagen, Hilden, Germany). Afterwards ...
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bioRxiv - Cancer Biology 2023Quote: ... washed in ice-cold PBS and resuspended in 350μl of Buffer RLT Plus supplemented with 1% (v/v) β-mercaptoethanol from the RNeasy Plus Mini Kit (Qiagen, 74134). RNA was extracted using the same kit according to manufacturer’s instructions and quality was assessed using the Agilent 2200 TapeStation System (Agilent ...
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bioRxiv - Molecular Biology 2023Quote: ... samples were lysed in RLT buffer supplemented with β-mercaptoethanol and RNA was purified using the RNeasy Mini Kit (#7410, Qiagen) with on-column DNase treatment (#79254 ...
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bioRxiv - Cell Biology 2023Quote: Total RNA from 1,000 H3K27me3 HI/LO β-cells or from 50,000 sorted βHI and βLO cells was extracted using the miRNeasy FFPE Kit (QIAGEN, 217504), followed by the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® (E6420L) ...
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bioRxiv - Bioengineering 2023Quote: ... samples were suspended in RNeasy kit Lysis Buffer and β-mercaptoethanol (1:100 v/v) and homogenized using QIAshredder columns (Qiagen). Samples loaded on spin columns were treated with DNase (Qiagen ...
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bioRxiv - Evolutionary Biology 2023Quote: ... RNA was extracted after disrupting the frozen leaves with metal beads in RLT and β mercaptoethanol solution using a Qiagen RNeasy plant mini kit (Qiagen) according to manufacturer’s instructions (without the optional DNase step) ...
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bioRxiv - Cell Biology 2024Quote: Cell pellets or lung lobes from individual mice were snap frozen in 350µl of 1:100 β-mercaptoethanol in the RLT Lysis Buffer from the RNAeasy mini plus Kit (Qiagen, 74134). Cell samples were thawed and vortexed while lung samples were homogenized for total RNA extraction intended for qPCR or bulk RNA-seq ...
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bioRxiv - Immunology 2024Quote: ... Cells were harvested and total RNA was extracted by lysing the cells with β-mercaptoethanol and a Rneasy Plus Mini Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Equalized lysates were then supplemented with β-mercaptoethanol (5mM) and imidazole (50mM) and incubated overnight with 20 µl (40 µl slurry) washed Ni-NTA beads (Qiagen). After washing with a wash buffer series (1x wash buffer 1 ...
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bioRxiv - Molecular Biology 2024Quote: Cells were lysed by directly adding 35 μl of β-mercaptoethanol-supplemented TCL buffer for mRNA extraction using TurboCapture 96 mRNA Kit (#72251, QIAGEN) following manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Cultured primary cells were rinsed once with ice-cold PBS and collected in freshly prepared extraction buffer consisting of 1% β- mercaptothanol in RLT (Qiagen). Saline-perfused ...
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bioRxiv - Immunology 2024Quote: SARS-CoV-2 specific T cell immune responses were evaluated by QuantiFERON SARS-CoV-2 (Qiagen) [36] ...
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bioRxiv - Genomics 2020Quote: ... 2 μL of Buffer EB (Qiagen), and 30 μL of Hybridization Enhancer ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mL Ni-NTA resin (Qiagen) was used for clarified cell lysate from 1 L bacterial culture ...
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bioRxiv - Microbiology 2022Quote: ... 2) the DNeasy PowerWater Kit (Qiagen), which is optimized for the isolation of genomic DNA from filtered water samples ...
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bioRxiv - Molecular Biology 2023Quote: ... and DNase I (2 mg (Qiagen) were dissolved in 30 mL of
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bioRxiv - Microbiology 2024Quote: ... 2 µg of RNase A (Qiagen) were added to each sample ...
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bioRxiv - Zoology 2024Quote: ... 2 µl 5x Q-Solution (QIAGEN), 1 µl primer mix (2 µM each primer) ...
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bioRxiv - Cancer Biology 2021Quote: ... and 3 μg target vector using PolyFect transfection reagent (Qiagen, 301107). Media containing virus was collected both 48 and 72 hours post-transfection and viral particles were precipitated using PEG-it™ Virus Precipitation Solution (System Biosciences ...
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bioRxiv - Molecular Biology 2021Quote: ... for 3 min at 50 Hz using a TissueLyser LT (Qiagen) with 1 min incubation on ice in between homogenisations ...
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bioRxiv - Microbiology 2020Quote: ... CjNC110-LNA DIG-labeled probe (/5’DigN/ GCACATCAGTTTCAT/3’Dig_N/) (QIAGEN) was added to 15 mL DIG EasyHyb™ Buffer (Roche ...
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bioRxiv - Systems Biology 2022Quote: ... 5 μl of proteinase K (approximately 3 U, Qiagen, cat. # 19131) were added and incubated for 1 h at 56°C ...
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bioRxiv - Neuroscience 2020Quote: ... siRNAs targeting the 3’UTR of Arhgap11a were purchased (siRNAflex, Qiagen).
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was incubated with 3 ml Ni-NTA resins (QIAGEN) with gentle agitation at 4 °C for an hour ...
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bioRxiv - Cell Biology 2021Quote: ... 5’-TAGCATTGTCAAGCTCCTAAA-3’ siRNAs or AllStars Negative Control siRNA (Qiagen, #1027281). After 72 h ...
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bioRxiv - Molecular Biology 2022Quote: ... Antisense LNA GapmeRs (custom designed, 3’-FAM-labeled, Qiagen, Table S5) were bilaterally microinfused using a 2 μl calibrated micropipette (Hamilton syringes ga 25/70mm/pst3) ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was incubated with 3 mL Ni-NTA agarose (Qiagen) pre-equilibrated with binding buffer (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Bioengineering 2023Quote: ... containing 3 μL 4X Probe PCR Master Mix (250102, Qiagen, USA) (final concentration 1X) ...
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bioRxiv - Cell Biology 2023Quote: ... using 30 nM Hs_LUZP1 siRNA (target sequence, 5′-CAGCGGGTGCTGAGAATTGAA-3′; QIAGEN) or 30 nM AllStars negative-control siRNA (QIAGEN) ...
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bioRxiv - Microbiology 2024Quote: ... and 3) DNA extraction (using here DNeasy Blood & Tissue kit, Qiagen) from the pellet for molecular analysis to detect E ...
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bioRxiv - Microbiology 2024Quote: ... The pellets were resuspended in 3 ml RNAprotect Bacteria Reagent (Qiagen), aliquoted ...
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bioRxiv - Cell Biology 2024Quote: ... HOXD11 (Qiagen, Sequence: 5′-TGC TAG CGA AGT CAG A-3′), HOXD13 (Qiagen ...
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bioRxiv - Cell Biology 2024Quote: ... HOXD13 (Qiagen, Sequence: 5′-CAT CAG GAG ACA GTA T-3′), for 24hr and 48hr for RNA and protein extraction respectively ...