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Citations for Qiagen :
751 - 800 of 3123 citations for 4 2 Hydroxyimino 2 4 methoxy phenyl ethyl piperazine 1 carboxylic acid tert butyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... with 200 µl of the resulting supernatant transferred into 2 ml EZ1 sample tubes (Qiagen). 12 µl of Proteinase K was added to the supernatant ...
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bioRxiv - Immunology 2023Quote: ... DNA was isolated for each fraction using EZ1&2 DNA Blood 350µL kits (Qiagen, Hilden) and the EZ1 Advanced XL automated system (Qiagen ...
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bioRxiv - Immunology 2022Quote: ... The initial homogenization was performed mechanically (2 metal beads/sample) using a TissueLyserLT (#85600, Qiagen) homogenizer (50 oscillations/s for 10 min) ...
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bioRxiv - Microbiology 2023Quote: ... MAYV samples were homogenized at 30 Hz for 2 min in a TissueLyser II (Qiagen) and centrifuged for 30 sec at 11,000 rpm ...
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bioRxiv - Genomics 2023Quote: ... RNAse A treatment was conducted by adding 2 uL of RNAse A 100ug/mL (Qiagen) and incubating the samples at 37°C for 15 min ...
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bioRxiv - Plant Biology 2024Quote: ... all samples were ground for 2 minutes at 25 Hz (TissueLyser II, QIAGEN, Hilden, Germany). A dilution series was performed on this mixture with MgCl2 (10 mM ...
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bioRxiv - Immunology 2024Quote: ... PCR products were purified from 2 % agarose-gels using QIAquick Gel Extraction Kit (QIAGEN, 28706) for Sanger sequencing (Microsynth).
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bioRxiv - Microbiology 2024Quote: ... The paper circles were transferred to PowerBead Pro Tubes (2 ml) (cat. no. 19301, Qiagen) containing pre-loaded homogenization beads.
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bioRxiv - Immunology 2024Quote: ... 2×106 MDSCs were used for RNA extraction using the Rnaeasy Plus Kit (Qiagen # 4134), according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... 2 µL of sonicated RNA per sample was purified with miRNeasy Micro Kit (Qiagen, 217084) and treated with DNase I (Qiagen ...
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bioRxiv - Cell Biology 2021Quote: ... and 1 mM PMSF and the resulting supernatant was incubated with nickel-nitrilotriacetic acid agarose (QIAGEN) overnight at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... The lysates were then incubated for 1 h with nickel-nitriloacetic acid (Ni-NTA) beads (Qiagen) at the indicated temperatures (4°C or room temperature) ...
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bioRxiv - Genomics 2023Quote: ... cfDNA was isolated from 1 mL of plasma using the QIAGEN Circulating Nucleic Acids Kit (QIAGEN), eluted in AE buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were harvested by centrifugation at 4°C followed by cell lysis/RNA extraction using RNEasy plus mini kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Total RNA of CDC-EVs (n=12) and MSC-EVs (n=4) was extracted using the miRNeasy Serum/Plasma kit (QIAGEN). Library construction was performed according to the manufacturer’s protocol using the TruSeq small RNA Library Kit (Illumina) ...
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bioRxiv - Cell Biology 2020Quote: ... lysed by sonication and the lysates were centrifuged at 52000 g for 45 min at 4°C.The supernatants were incubated with Ni-NTA agarose resin (Qiagen, Germany) for 2 hours before washing with 100 bed volume buffer A supplemented with 50mM imidazole ...
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bioRxiv - Genetics 2021Quote: ... 12 WT littermates) and two-month-old mice (adult, n = 4 per group) were lysed and homogenized in QIAzol Lysis Reagent (Qiagen). Total RNA purification was performed with the miRNeasy Micro Kit (Qiagen ...
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bioRxiv - Developmental Biology 2021Quote: ... E18.5 RNA was extracted from cortices of 3 genotypic conditional knockouts and 4 controls using the RNeasy Micro Kit (Qiagen). RNA was reverse transcribed to cDNA using the SuperScript™ II Reverse Transcriptase Kit (Invitrogen) ...
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bioRxiv - Genomics 2020Quote: ... We stored tissue at 4 °C for 24-72 hours prior to extracting DNA with a DNAeasy Blood and Tissue Kit (Qiagen) with on-column RNase A treatment following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... The insoluble fraction was precipitated by ultracentrifugation (20,000 g) for 30 minutes at 4°C and the supernatant was loaded onto a Ni-NTA superflow affinity column (QIAGEN). The Ni-column was then wash three times and eluted with buffer containing 30 mM HEPES (pH 7.8) ...
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bioRxiv - Biochemistry 2021Quote: ... the cell lysate was cleared by centrifugation (20000 rpm, 30 minutes, 4 °C) and purified using Ni2+-affinity chromatography (Ni-NTA superflow cartridges, Qiagen). Typically two 5 mL columns were used for a 2 L culture in which the lysate was loaded on the column washed with 10 CV wash buffer (50 mM Hepes ...
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bioRxiv - Biochemistry 2021Quote: ... the cell lysate was cleared by centrifugation (20000 rpm, 30 minutes, 4 °C) and purified using Ni2+-affinity chromatography (Ni-NTA superflow cartridges, Qiagen). Typically two 5 mL columns (flow 5 mL/min ...
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bioRxiv - Cancer Biology 2021Quote: ... a cancer associated fibroblast (CAF) cell line (pCAF2) expressing TGF-β responsive SMAD2/3/4 RE-Luciferase (Qiagen, #CLS-017L) was created ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells attached to the collagen matrix were fixed with 4% PFA for 10 minutes at room temperature for IF analysis or lysed with RLT buffer (Qiagen) for RNA isolation and qRT-PCR analysis as described above.
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bioRxiv - Microbiology 2020Quote: ... Media was refreshed 4 and 8dpi and surviving cells were collected 12dpi for genomic DNA extraction using QIAamp DNA Blood Maxi Kit (Qiagen). gRNA encoding DNA sequences were amplified in a two-step nested PCR using KAPA HiFi HotStart ReadyMixPCR Kit (Kapa Biosystems ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA (2000 ng) was transcribed into cDNA in a volume of 20 μl using 4 U Omniscript reverse transcriptase (Qiagen). To determine the relative abundance of the mRNAs of interest ...
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bioRxiv - Microbiology 2021Quote: ... The incubation period at 56°C was set to one hour for all samples and all samples were treated with 4 µl RNase A (100 mg/ml, Qiagen). DNA was eluted in 100 µl TE buffer with 0.1 mM EDTA ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was isolated from pools of 6-10 dissected hearts of 4-OHT-treated cmcl2/RFP and cmlc2/GFP-HRAS juvenile fish that were homogenized in Qiazol (Qiagen) with a TissueLyser (Qiagen) ...
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Pou3f1 orchestrates a gene regulatory network controlling contralateral retinogeniculate projectionsbioRxiv - Developmental Biology 2022Quote: ... Sorted ßGal+ or GFP+ cells were collected in 1.5ml DNA LoBind® tubes placed at 4°C containing 400µl RLT buffer (Qiagen, 79216) with 1% β-Mercaptoethanol (Millipore Sigma ...
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bioRxiv - Microbiology 2022Quote: ... Nasal turbinates and lungs were collected from a subset of hamsters at 4 dpi and homogenized in 1ml or 5 ml of PBS with TissueRuptor (Qiagen), respectively ...
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bioRxiv - Molecular Biology 2022Quote: His-tagged G5845–1508 (His-G5845–1508) (4 pmol) were coupled to Ni-agarose resin (25 μl of beads suspension) (Qiagen) during 1 h ...
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bioRxiv - Plant Biology 2021Quote: Total RNA was extracted from the leaves of 4-to-6-week old plants using the RNeasy Plant Mini Kit (Qiagen). RNA concentrations were quantified using a Qubit RNA BR Assay Kit (ThermoFisher ...
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bioRxiv - Plant Biology 2021Quote: ... We extracted total RNA from 10 leaves that were shorter than 500 µm and from 4–6 mature leaves using the RNeasy Micro Kit (Qiagen) and the RNeasy Plant Mini Kit (Qiagen) ...
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bioRxiv - Neuroscience 2021Quote: ... total RNA was isolated from laser-microdissected control and mutant samples (n = 4) using the RNeasy Micro Plus Kit (Qiagen). The isolated RNA was checked for purity and integrity using Nanodrop spectrophotometer and TapeStation (Agilent) ...
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bioRxiv - Microbiology 2021Quote: ... and processed for sampling within 4 hours of collection using the MO BIO PowerSoil DNA Isolation Kit (QIAGEN; Germantown, MD). DNA in the samples was amplified via PCR with primers targeted to the V1-V3 region of the 16S rRNA ...
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bioRxiv - Cancer Biology 2021Quote: ... Genomic DNA was isolated from liver and lung tissues (n=4 for MEL077 and n=6-7 for MP46 per treatment group) using the QIAamp DNA Mini Kit (Qiagen) and for blood (n=3-4 for MEL077 and n=6-7 for MP46 per treatment group ...
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bioRxiv - Cancer Biology 2021Quote: ... and for blood (n=3-4 for MEL077 and n=6-7 for MP46 per treatment group) using the QIAamp DNA Blood Mini kit (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: Total RNA was extracted from the stomach and pyloric caeca tissue samples stored at -80 °C (n = 4 for control and fly larvae, n = 5 for shrimp shell) using the RNeasy Plus Universal Kit (QIAGEN). RNA quality was assessed using a 2100 Bioanalyzer with the RNA 6000 nano kit (Agilent ...
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bioRxiv - Developmental Biology 2022Quote: Total RNA from sorted CD45-EpCam+UEA1+ and CD45-EpCam+UEA1-thymic stromal cells from 4-week-old mice were isolated using the RNEasy kit (Qiagen). cDNA was synthesized from the total RNA and used as a template for real-time relative quantitation for Foxn1 using commercially available probes and reagents (Invitrogen ...
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bioRxiv - Immunology 2020Quote: RNA from isolated fresh B cells and B cells activated with LPS/IL-4 was extracted using a RNeasy kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... and adult females (approximately 4-5 of each) were prepared for RNAseq using the RNeasy Blood and Tissue Kit (Qiagen). Individuals were placed in a 1.5 mL safelock tube along with 5-8 one mm glass beads placed in liquid nitrogen and then shaken for 30 s in a Silamat S6 shaker (Ivoclar Vivadent) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were pelleted at 350 x g for 5 min at 4 °C and lysed in 350 μl RLT buffer of the RNeasy Mini Kit (Qiagen). RNA was isolated according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared by centrifugation at 20,000 rpm for 1 hour at 4°C and the soluble fraction was affinity purified by gravity column with Ni-NTA affinity resin (QIAGEN). The protein was eluted by 50 mM Tris pH 8 ...
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bioRxiv - Microbiology 2021Quote: ... The lysate was clarified by centrifugation at 10,000 × g for 30 mins at 4 °C and the supernatant was applied to an Ni-NTA affinity column according to the manufacturer’s instructions (Qiagen, Germany). Bound proteins were washed twice with wash buffer (50 mM NaH2PO4 ...
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bioRxiv - Genetics 2020Quote: ... coli strains expressing (His)6-Clr4 and (His)6-Swi6 and allowed to bind at 4°C overnight to Ni-NTA resin (Qiagen; binding capacity 5-10mg protein/ml resin) ...
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bioRxiv - Physiology 2021Quote: ... was homogenized using the Fisherbrand Bead Mill 4 and total RNA was extracted from homogenates using the RNeasy Tissue Mini Kit (Qiagen), according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... 80 µL of the mixture was then incubated for 45-60 minutes at room temperature or 4°C with 10 µL NiNTA resin (Qiagen) prewashed with His-SUMO Lysis buffer (20 mM Tris-HCl ...
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bioRxiv - Neuroscience 2021Quote: Total RNA from 700,000 to 1.25 million RGCs from 4 independent rat litters per developmental stage was isolated using the Qiagen RNAeasy kit from Qiagen, which included a 15 minutes On-column DNAse step ...
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bioRxiv - Genomics 2021Quote: Strains were grown overnight in 50 ml YPD broth and genomic DNA was extracted from approximately 4×109 cells using a QIAGEN Genomic-tip 100/G kit (10223, QIAGEN) with minor modifications ...
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bioRxiv - Cancer Biology 2021Quote: ... 250 to 1000 μm lengths of epithelium sections were microdissected from 4 to 6 successive serial sections and DNA extracted using a QIAMP DNA microkit (Qiagen) by digesting overnight and following manufacturer’s instructions ...