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Citations for Qiagen :
601 - 650 of 3140 citations for Carbamic acid 2 4 hexadienyl 1 1 dimethylethyl ester E E 9ci since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... S1 RBD was purified from the culture supernatant by nickel–nitrilotriacetic acid agarose (Qiagen), and purity was confirmed to by >95% as judged by coomassie stained SDS-PAGE ...
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bioRxiv - Plant Biology 2020Quote: ... His-tagged proteins were incubated with Nickel-nitrilotriacetic acid (NTA) sepharose (Qiagen, Hilden, Germany) at 4°C with slight shaking for 1 h ...
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bioRxiv - Plant Biology 2020Quote: ... coli Rosetta cells was carried out using nickel-nitrilotriacetic acid (Ni-NTA) agarose (Qiagen) as described in Barja and Rodríguez-Concepción (2020) ...
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bioRxiv - Molecular Biology 2021Quote: ... we added 70 µl of Ni-NTA (Nickel Nitrilo-triacetic Acid) agarose beads (Qiagen), and incubated the mixture overnight at the orbital rotator at RT ...
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bioRxiv - Microbiology 2020Quote: ... 0.5 ml of culture supernatant was incubated with nickel-nitrilotriacetic acid-agarose beads (QIAGEN). The beads were washed ...
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bioRxiv - Molecular Biology 2023Quote: ... After purification of AtH2B.9 using nickel-nitrilotriacetic acid agarose (Ni-NTA) resin (QIAGEN), the His6-tag portion was removed by TEV protease (30 µg/mg of His6-H2B.9) ...
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bioRxiv - Microbiology 2023Quote: Nucleic acid extraction was carried out using DNeasy PowerSoil Pro Kit (QIAGEN, Hilden, Germnay) following manufacturer instructions ...
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bioRxiv - Immunology 2024Quote: ... Proteins were purified from filtered supernatants using nickel nitriloacetic acid (Ni-NTA) agarose (QIAGEN) via gravity flow ...
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bioRxiv - Developmental Biology 2023Quote: ... specific locked nucleic acid (LNA) probes doubly-labelled with digoxigenin were purchased from Qiagen and ISH was conducted following the published protocol (Chen et al. ...
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Safe plant Hsp90 adjuvants elicit an effective immune response against SARS-CoV2 derived RBD antigenbioRxiv - Molecular Biology 2023Quote: ... Soluble RBD was purified under denaturing conditions using a nitrilotriacetic acid-Ni2+ column (Qiagen)[19] ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatant was loaded onto a nickel-nitriloacetic acid (Ni2+-NTA) column (Qiagen, Hilden, Germany), washed with 500ml buffer A ...
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bioRxiv - Microbiology 2022Quote: ... Supernatant was applied to a 5 ml nickel-nitrilotriacetic acid column (Qiagen, Valencia, CA) that had been equilibrated with buffer A at a rate of 1 ml/min ...
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bioRxiv - Biophysics 2022Quote: The obtained supernatant was incubated with Ni-nitrilotriacetic acid (Ni-NTA) resin (Qiagen, Germany) in a rotating shaker for 1 h ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The clarified lysate was incubated with nickel-nitrilotriacetic acid (Ni-NTA) agarose resin (Qiagen) for 1 hour with agitation ...
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bioRxiv - Synthetic Biology 2023Quote: ... the clarified lysate was loaded on a Ni-nitrilotriacetic acid (NTA) agarose column (Qiagen) using the ÄKTA Explorer fast protein liquid chromatography system (FPLC ...
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bioRxiv - Immunology 2024Quote: ... Total RNA is isolated using the column based nucleic acid purification kit (RNeasy, Qiagen), strictly according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... the supernatant was incubated with 3 ml nickel- nitrilotriacetic acid Ni-NTA beads (Qiagen) preequilibrated with the same buffer overnight ...
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bioRxiv - Microbiology 2024Quote: ... Lysates were subjected to pull down using nickel-nitrilotriacetic acid (Ni-NTA) beads (Qiagen) overnight at 4 °C on a rotation platform ...
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bioRxiv - Bioengineering 2024Quote: ... Clarified supernatants were incubated overnight with Ni-nitrilotriacetic acid (Ni-NTA) resin (Qiagen, Germany) with constant stirring at 4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... 5.6 x 105 cells were seeded in 4 ml DMEM in a 6-cm culture plate and transfected with targeting or control siRNA (Table 4) (from Qiagen) to a final concentration of 20 nM for each siRNA used ...
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bioRxiv - Cell Biology 2021Quote: ... of healthy (4 weeks of age) and hTNFtg mice (4 & 8 weeks of age) using the RNeasy mini or micro kit (QIAGEN), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: SUZ12 isogenic M3 MPNST cells were treated with or without DNMT inhibitors (50 nM daily Decitabine or 4 μM single dose of GSK862) for 4 days followed by genomic DNA isolation with Gentra Puregene cell kit (Qiagen). Bisulfite sequencing was conducted by the IGO core facility at MSKCC ...
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bioRxiv - Cell Biology 2023Quote: ... ULK2 (mixture of 4 siRNAs, GS9706), TBC1D14 (mixture of 4 siRNAs, GS57533) and non-targeting (NT, # 5091027310) were purchased from Qiagen.
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bioRxiv - Biochemistry 2020Quote: ... 3-4 mL of Ni-NTA resins (Qiagen) were applied on the gravity column ...
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bioRxiv - Molecular Biology 2020Quote: ... RNase Inhibitor (4 unit/µl) (cat # 1055213, Qiagen), rRNasin Rnase inhibitor (40 U/µl ...
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bioRxiv - Molecular Biology 2021Quote: ... and 4) DNeasy Plant Mini Kit (QIAGEN®) combined with the InhibitEx Buffer step from the QIAamp DNA Stool Mini Kit to improve inhibitor removal ...
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bioRxiv - Immunology 2021Quote: ... 3-4 pieces were placed into RNAlater (Qiagen) for gene expression analysis ...
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bioRxiv - Synthetic Biology 2022Quote: ... 4 μL of 100 mg/mL RNase (Qiagen) was added ...
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bioRxiv - Microbiology 2024Quote: ... RNase A (4 µL, 100 mg/mL, Qiagen) was added to remove RNA and incubated at 25°C for 2 min ...
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bioRxiv - Microbiology 2024Quote: ... after 4 h of coculture (Qiagen, Montreal, Canada). Reverse transcription of isolated RNA was performed using the Maxima First Strand Kit (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... respectively34 (Table 1) were analyzed on the Rotor-GeneQ machine (Qiagen) using the following PCR conditions ...
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bioRxiv - Cell Biology 2022Quote: To synthesize cDNA from 1 μg RNA the Quantitect kit (Qiagen) was used according to manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... 1% SDS) supplemented with 2µl of 0.5mg/ml RNase A (QIAGEN) at 55°C for 2 hours ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... or Junctional Adhesion Molecule 1 (JAM1) using Polyfect (Qiagen, CA, USA) and selected in 1000 µg/ml G418 for stable transfectants ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was applied to 1 ml Ni-NTA Agarose (Qiagen) equilibrated with lysis buffer ...
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bioRxiv - Microbiology 2020Quote: ... the supernatant was decanted and 1 mL QIAzol Lysis Reagent (QIAGEN) was added to resuspend the cell pellet and protect the RNA ...
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bioRxiv - Microbiology 2020Quote: ... PCR#1 product was purified using Minielute PCR purification kit (Qiagen) and a second round of PCR amplification was performed with gene-specific primers R2 and forward F2 ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteinase digestion was performed with 1 mg/mL proteinase K (Qiagen) in the presence or absence of 1% Triton X-100 for 30 min at 37 °C as reported previously64 ...
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bioRxiv - Cell Biology 2022Quote: ... added to 1 ml of washed Ni2+-NTA agarose (Qiagen, #1018244) and rotated on a low speed wheel for at least 1h at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... Homogenization was performed after adding 1 ml of inhibitex buffer (Qiagen) using the Bead Ruptor Elite bead mill homogenizer (Omni ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 ml binding buffer (provided in Qiagen PCR purification kit, #28106) and 20 µl of 3 M sodium acetate pH 5.5 (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was added to 1 mL Ni2+-NTA resin (Qiagen) and incubated for 1 hr at 4 °C ...
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bioRxiv - Biophysics 2022Quote: ... Lysates were incubated with 1 ml of Ni-NTA resin (QIAGEN) in 4°C for 1 hr ...
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bioRxiv - Genomics 2020Quote: ... Supernatant was loaded on two 1-ml Ni-NTA (Qiagen 30210) columns ...
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bioRxiv - Physiology 2021Quote: ... and 1% β-mercaptoethanol with a TissueLyser II (Qiagen, Manchester, UK) at maximum speed for 2 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... then diluted to 1 × 109 with 180 μL Buffer AE (Qiagen). Standard curves were prepared from 1 × 107 copies/rxn to 1 × 101 copies/rxn ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 1 colonies using a DNeasy Plant Mini Kit (QIAGEN, Hilden, Germany). We used DNeasy Blood & Tissue Kits (QIAGEN ...
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bioRxiv - Microbiology 2023Quote: ... plus 1% ꞵ-mercaptoethanol and homogenized using a QIAshredder column (QIAGEN). RNA was extracted using the RNeasy Mini kit (QIAGEN) ...
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bioRxiv - Physiology 2023Quote: ... The tissue lysates were treated with 1% DNase (Qiagen, Hilden, Germany) and diluted to a protein concentration of 10μg/ml ...
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bioRxiv - Microbiology 2023Quote: ... then transferred to 1 ml RNAprotect Tissue Reagent (Qiagen, Hilden, Germany) for colonic transcript analyses ...