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Citations for TianGen Biotech :
1 - 29 of 29 citations for Sitagliptin Impurity 02 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... gDNA buffer was added into total RNA to remove DNA impurity and then the purified RNA was used as a template to synthesized cDNA using a FastKing RT Kit (TIANGEN). The RT-qPCR assays were performed on qTOWER3 Real-Time PCR thermal cyclers (Analytik Jena ...
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bioRxiv - Microbiology 2024Quote: ... 100 μL of TMB peroxidase substrate (TIANGEN, PA107-02) was added to each well and incubated for 15 min before being stopped by adding 2 M H2SO4 ...
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bioRxiv - Developmental Biology 2023Quote: ... NF-H2O) and 10 μL proteinase K (20 mg/mL) (RT403-02, TIANGEN) was added to the slide and incubated for 10 min at room temperature ...
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bioRxiv - Synthetic Biology 2021Quote: ... and the DNA is extracted using the TIANgeo Midi Purification Kit (TIANGEN, DP209-02) or AxyPrep DNA gel Extraction Kit (AXYGEN ...
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bioRxiv - Genetics 2024Quote: ... Quantitative PCR was carried out using SuperReal PreMix Plus (SYBR Green) (Tiangen #DP4-02). RP49 were used to normalize results between groups ...
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bioRxiv - Microbiology 2023Quote: ... Each reaction contained 25 ul of SuperRealPreMix Plus SYBR Green (Tiangen Biotechnology, Beijing, FP205-02), one μl of cDNA ...
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bioRxiv - Genetics 2024Quote: Total RNA was extracted from cells and tissues using TRNzol Universal Reagent (Tiangen #DP4-02). 18S rRNA was obtained through polyacrylamide gel electrophoresis and gel recovery of total RNA ...
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bioRxiv - Synthetic Biology 2021Quote: ... plasmid extraction was performed following the manuals of the TIANprep Mini Plasmid Kit (TIANGEN, DP103-02) or AxyPrep Plasmid Miniprep Kit (AXYGEN ...
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bioRxiv - Microbiology 2020Quote: ... All the DNA samples were purified through a DNA kit column (DP214-02, Tiangen, Beijing, China) and kept at −20°C until further analysis (6) ...
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bioRxiv - Immunology 2020Quote: ... and total bacterial DNA was extracted using a bacterial DNA extraction kit (DP302-02, Tiangen, China) as per the manufacturer’s protocols ...
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bioRxiv - Genetics 2024Quote: Total RNA was extracted from cells and tissues using the TRNzol Universal Reagent (Tiangen #DP4-02). RNA reverse transcription was performed by using PrimeScript RT reagent Kit with gDNA Eraser (TAKARA #RR047A) ...
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bioRxiv - Cell Biology 2024Quote: ... the chromatin sample concentration was assessed by the BCA Protein Assay Kit (Tiangen, cat no. PA115-02). 1 μg H3K27ac antibody (Abcam ab4729 ...
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bioRxiv - Biochemistry 2021Quote: ... An aliquot of 0.5 μg RNA were reverse transcribed to cDNA by FastKing RT Kit (KR116-02, Tiangen). cDNA samples were used as templates to perform real- time quantitative PCR with the power SYBR green mix (Abm ...
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bioRxiv - Immunology 2022Quote: ... cell pellets were re-suspended in 5 mL of red blood cell lysis buffer (RT122-02, TIANGEN, China) for 5 min at RT for remove the red blood cell ...
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bioRxiv - Molecular Biology 2020Quote: ... and cDNA transcription was accomplished using a first strand cDNA synthesis kit (Cat# KR116-02, Tiangen Biotech, China). Subsequently ...
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bioRxiv - Bioengineering 2021Quote: PC12 cell or rat liver gDNAs was isolated using the TIANamp blood DNA kit (TIANGEN Biotech, DP304-02) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cell pellets were re-suspended in 3 mL of red blood cell lysis buffer (RT122-02, TIANGEN, China) for 5 min at RT to remove the red blood cells ...
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bioRxiv - Synthetic Biology 2021Quote: ... The DNA fragments were separated by agarose gel electrophoresis and purified using a TIANgeo Midi Purification Kit (TIANGEN, DP209-02) or AxyPrep DNA gel Extraction Kit (AXYGEN ...
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bioRxiv - Microbiology 2020Quote: ... Total genomic DNA from each sample was extracted using the TIANamp Bacteria DNA isolation kit (DP302-02, Tiangen, Beijing, China). The quality of the extracted DNA was monitored on 1% agarose gels using electrophoresis and spectrophotometry (optical density at 260/280 nm ratio) ...
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bioRxiv - Molecular Biology 2020Quote: ... quantitative real-time PCR (qRT-PCR) was performed with SYBR green as fluorescence dye (Cat# FP205-02, Tiangen Biotech, China) in a total reaction volume of 20 μL according to the standard manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Protein concentrations were determined using the BCA Protein Assay Kit according to the manufacturer’s instructions (TIANGEN, catalog number: PA115-02).
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bioRxiv - Evolutionary Biology 2024Quote: ... was extracted from transfected HEK-293T from each subline (1×105) with the TIANamp genomic DNA Kit (DP304-02; Tiangen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Genome DNA from the collected cells of P2 was extracted under the instructions of the user’s manual for TIANamp Genomic DNA Kit (TIANGEN, DP304-02). PCR was later conducted to amplify mitochondrial COI gene fragment using the primer pair as follows ...
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bioRxiv - Plant Biology 2021Quote: ... and the leaf samples were pooled for each group for DNA extraction using the TIANGEN DNA purification kit (TIANGEN, DP305-02). High-throughput sequencing and Bulked Sequence Analysis (BSA ...
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bioRxiv - Genomics 2022Quote: ... on an agarose gel and were subsequently extracted using a Universal DNA purification Kit (Catalog No. DP214-02, TIANGEN, Beijing, China) and then used as a template to synthesize dsRNA with a MEGAscript RNAi Kit (Catalog No ...
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bioRxiv - Synthetic Biology 2021Quote: ... The incubated solution is then subjected to agarose gel electrophoresis and the correct band is extracted using a TIANgeo Midi Purification Kit (TIANGEN, DP209-02) or AxyPrep DNA gel Extraction Kit (AXYGEN ...
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bioRxiv - Synthetic Biology 2021Quote: ... The incubated solution is then subjected to agarose gel electrophoresis and the correct band is extracted using a TIANgeo Midi Purification Kit (TIANGEN, DP209-02) or AxyPrep DNA gel Extraction Kit (AXYGEN ...
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bioRxiv - Microbiology 2022Quote: ... The target N gene of GX_P2V isolate was amplified by qPCR using FastFire qPCR PreMix (Probe) (TIANGEN, Cat No. FP208-02, Beijing, China) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... 1 μg of purified RNA was reverse-transcribed in a 20 µL reaction using a FastQuant RT Super Mix Kit (TIANGEN, catalog no. KR106-02). Real-Time PCR was then performed by using Maxima SYBR Green/ROX kit (ThermoFisher Scientific ...