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Citations for TianGen Biotech :
1 - 25 of 25 citations for Recombinant Human SPARC like 1 Hevin His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... His-tag antibody (Tiangen, Beijing, China) and GST antibody (Tiangen).
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bioRxiv - Genomics 2023Quote: ... Total genomic DNA was extracted from fresh leaves using the Hi-DNAsecure Plant Kit (TIANGEN, China). Then ...
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bioRxiv - Bioengineering 2023Quote: The recombinant plasmids were extracted using the TIANprep Rapid Mini Plasmid Kit (TIANGEN, Beijing, China). Genomic DNAs of bacteria were extracted using the Wizard Genomic DNA Purification Kit (Promega ...
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bioRxiv - Genomics 2023Quote: ... the genomic DNA was extracted from young leaves using the Hi-DNAsecure Plant Kit (cat. no. DP350; TIANGEN, China). Then ...
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bioRxiv - Microbiology 2020Quote: ... The PCR product was purified and directly recombined by the EasyGeno recombinant clone kits (Cat:VI201, Tiangen). After conventional transformation and monoclonal screening of positive bacteria ...
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bioRxiv - Plant Biology 2021Quote: Sequences for H2B.8 and H2B.2 were cloned into the pET28a+ vector with a non-cleavable C-terminal 8×His-tag and then transformed to Escherichia coli strain BL21 (Tiangen). Cells were grown to OD 0.8 at 37 °C in LB media with 30 μg/ml kanamycin ...
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bioRxiv - Microbiology 2023Quote: PTrc His-A vector harboring various JEV NS2B-NS3 proteases were propagated in Escherichia coli DH5 (TIANGEN Biotech, Beijing, China) and extracted using Plasmid Miniprep Kit (Corning ...
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bioRxiv - Immunology 2024Quote: ... The correct construction of the recombinant plasmids was verified through Sanger sequencing and extracted using an Endotoxin-Free Plasmid DNA Miniprep Kit (Tiangen, China) before using plasmids ...
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bioRxiv - Cancer Biology 2023Quote: ... Human genome DNA extracted from HCT116 cells using the TIANamp Genomic DNA Kit (Tiangen Biotech, Beijing, China) was used as template for amplifying the promoter regions ...
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bioRxiv - Cancer Biology 2021Quote: The RM-1 cells of 1×107 WT and Mettl9 KO were extracted with TRNzol (TIANGEN) for total RNA library preparation ...
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bioRxiv - Biochemistry 2020Quote: ... mixed with chloroform (1:1 v/v) using tubes with phase lock gel (Tiangen WM5-2302831). The aqueous phase was then mixed with 0.2 volume of 10 M ammonium acetate and 1 volume of isopropanol ...
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bioRxiv - Cell Biology 2022Quote: ... 1 unit of Taq DNA polymerase (TIANGEN, China), and 1 × PCR buffer ...
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bioRxiv - Immunology 2022Quote: ... Catch buffer: to 1 ml of RNAase-free water (Tiangen Biotech), add 40 µl Rnasin (NEB ...
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bioRxiv - Developmental Biology 2023Quote: Tissue and cells were homogenized with 1 mL TRIZOL (TIANGEN, DP424), after which 200 μL of chloroform was added to the lysates for phase separation ...
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bioRxiv - Cancer Biology 2020Quote: ... isopropyl β-D-1-thiogalactopyranoside (IPTG) and phenylmethane sulfonyl fluoride were purchased from Tiangen Biotech Co. ...
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bioRxiv - Immunology 2022Quote: ... cDNA synthesis was executed with 1 µg of total RNA with BcaBEST RNA PCR Kit (TIANGEN, China) as the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Total RNAs (1 μg) of experimental cells were extracted with the RNA simple kit (Tiangen, Beijing, China) and added to the reverse transcriptase reaction to obtain the first strand of cDNAs by using RevertAid First Strand cDNA Synthesis kit (K1622 ...
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bioRxiv - Plant Biology 2020Quote: ... AAA-CAA) within the PGEX-6P-1 plasmid was performed using the fast Site-Directed Mutagenesis Kit (Tiangen, China, Catalog No. KM101). PCR was conducted using site-specific primers (Supplemental Table S17) ...
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bioRxiv - Cancer Biology 2022Quote: ... Total RNA (1 µg) was used as a template for cDNA synthesis with a QuantScript RT Kit (KR103; Tiangen Biotech, Beijing, China). To determine mRNA expression levels ...
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bioRxiv - Bioengineering 2019Quote: ... The cell suspension was centrifuged for 5 minutes at 12,000 rpm and cell precipitate was resuspend in 1 ml sterilizing saline used for DNA extraction with TIANamp Genomic DNA kit (TianGen Biotechnologies, Beijing). Subsequently ...
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bioRxiv - Plant Biology 2022Quote: ... total RNAs isolated from the leaves of SC8 and mutant lines (#1, #2, #3, #4) using RNAplant Plus reagent (TianGen, Beijing, China) following the manufacturer’s instructions were reversed by using the reverse transcriptase kit (TaKaRa ...
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bioRxiv - Cancer Biology 2023Quote: RNA was isolated from 1×106 THP1 cells after various treatments (see above) by using an RNAprep pure cell/bacteria kit (Tiangen Biotech 4992235) according to the manufacturer’s instructions and was quantified using NanoDrop OneC Spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... the DNA fragments were separated by 1.0 % agarose gel electrophoresis (1 × TAE buffer) and purified from the gel with the DNA Gel Extraction Kit (TianGen Biotech., Beijing, China), then they were sub-cloned into pCE2 TA/Blunt-Zero vector (Vazyme ...
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bioRxiv - Plant Biology 2020Quote: ... Genomic DNA was extracted from the leaves of T3 transgenic plants (Table S8, lines 1954-1 and 1955-3) using a plant genomic DNA Extraction Kit (Tiangen Biotech, Beijing, China). About 20 μg of DNA was successfully digested with 5 U of EcoRV and incubated at 37 °C for 24 h ...
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bioRxiv - Microbiology 2022Quote: ... at which point 1 mL of cells were harvested and RNA samples were extracted using the RNAprep Pure Bacteria Kit (Tiangen Co., Beijing, China). Total cDNA was generated from 500 ng of total template RNA using the HiScript III All-in-one RT SuperMix Perfect for qPCR (Vazyme Co. ...