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Citations for TianGen Biotech :
1 - 25 of 25 citations for Recombinant E. coli NAGK Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Zoology 2021Quote: ... Bacterial genomic DNA (R. a. and E. coli) were extracted using TIANamp Bacteria DNA Kit (Tiangen, Beijing, China). All DNA and cDNA were stored at −80°C until for use.
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bioRxiv - Biochemistry 2022Quote: ... coli (TIANGEN®). Site-directed mutagenesis was confirmed via plasmid sequencing (Synbio Technologies) ...
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bioRxiv - Biochemistry 2022Quote: ... coli (TIANGEN®), followed by purification and analyses as previously described.
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bioRxiv - Microbiology 2019Quote: ... coli DH5α competent cells (Tiangen). Four clones from each VP1 amplicon were picked for Sanger sequencing ...
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bioRxiv - Bioengineering 2023Quote: ... coli DH5α competent cells (Tiangen). Recombinant plasmids were prepared by using the QIAprep Spin Miniprep Kit (QIAGEN ...
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bioRxiv - Biochemistry 2022Quote: ... BL21 (DE3) Escherichia coli (TIANGEN®) transformed with the construct was grown in 450 mL LB supplemented with 50 μg/mL kanamycin at 37 °C ...
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bioRxiv - Bioengineering 2023Quote: ... coli BL21(DE3) competent cell (Tiangen) for protein expression.
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bioRxiv - Synthetic Biology 2022Quote: ... coli strain Top10 (TIANGEN biotech., Beijing, China). Single colonies were inoculated 2XYT medium with antibiotics at 37 °C ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli BL21(DE3) strains (TIANGEN and TSINGKE) and regulate protein expression level by lacO ...
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bioRxiv - Physiology 2020Quote: ... coli were extracted by the kit by HighPure Maxi Plasmid Kit (TIANGEN Biotech (Beijing) Co.,Ltd. ...
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bioRxiv - Bioengineering 2023Quote: The recombinant plasmids were extracted using the TIANprep Rapid Mini Plasmid Kit (TIANGEN, Beijing, China). Genomic DNAs of bacteria were extracted using the Wizard Genomic DNA Purification Kit (Promega ...
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bioRxiv - Physiology 2022Quote: ... Protein concentration was determined using BCA Protein Assay Kit (TIANGEN) and diluted in loading dye (β-mercaptoethanol 5% ...
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bioRxiv - Microbiology 2020Quote: ... The PCR product was purified and directly recombined by the EasyGeno recombinant clone kits (Cat:VI201, Tiangen). After conventional transformation and monoclonal screening of positive bacteria ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were quantified using a BCA Protein Assay Kit (TIANGEN, China). Western blotting analysis was performed according to previous studies [28 ...
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bioRxiv - Biochemistry 2020Quote: ... Protein concentration was measured using Bradford protein assay kit (Tiangen Biotech, Beijing Co., LTD, CN). The purity of the PM was determined by standard marker assays[32] ...
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bioRxiv - Plant Biology 2021Quote: Sequences for H2B.8 and H2B.2 were cloned into the pET28a+ vector with a non-cleavable C-terminal 8×His-tag and then transformed to Escherichia coli strain BL21 (Tiangen). Cells were grown to OD 0.8 at 37 °C in LB media with 30 μg/ml kanamycin ...
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bioRxiv - Microbiology 2023Quote: PTrc His-A vector harboring various JEV NS2B-NS3 proteases were propagated in Escherichia coli DH5 (TIANGEN Biotech, Beijing, China) and extracted using Plasmid Miniprep Kit (Corning ...
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bioRxiv - Immunology 2024Quote: ... The correct construction of the recombinant plasmids was verified through Sanger sequencing and extracted using an Endotoxin-Free Plasmid DNA Miniprep Kit (Tiangen, China) before using plasmids ...
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bioRxiv - Zoology 2020Quote: ... The recombinant plasmids pET-30a-RhATG5 and pET-30a-RhATG5191-199Δ were constructed and transformed into BL21 (DE3) competent Escherichia coli cells (Tiangen, Beijing, China). To induce the expression of the recombinant proteins RhATG5 and RhATG5191-199Δ ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... a BCA protein assay kit (TIANGEN, Beijing, China) was used to measure the membrane protein concentration ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were detected using an ECL fluorescence colorimetric kit (Tiangen) and visualized using a Bio-Rad Gel Doc XR ...
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bioRxiv - Neuroscience 2023Quote: ... Total protein concentration was measured using a BCA assay kit (TIANGEN). Then 10 mg of protein per sample was separated on 12% SDS-PAGE gels (Sangon Biotech ...
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bioRxiv - Cell Biology 2023Quote: ... the chromatin sample concentration was assessed by the BCA Protein Assay Kit (Tiangen, cat no. PA115-02). 1 μg H3K27ac antibody (Abcam ab4729 ...
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bioRxiv - Biochemistry 2024Quote: ... The total protein in the supernatant was quantified using the bicinchoninic acid assay (BCA) kit (Beijing TianGen Biotechnology Co., Ltd.). For each sample ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was isolated from single third-instar larva of three strains (total 30 larvae of each strain) using the DNA/RNA/Protein Isolation Kit (TianGen, Beijing, China). The first-strand cDNA was synthesized with 1 μg of total RNA using a PrimeScript™ 1st Strand cDNA Synthesis Kit with gDNA Eraser (TaKaRa Biotechnology ...