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Citations for TianGen Biotech :
1 - 30 of 30 citations for Leptospira Biflexa Antigen Strain Patoc 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... coli strain Top10 (TIANGEN biotech., Beijing, China). Single colonies were inoculated 2XYT medium with antibiotics at 37 °C ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli BL21(DE3) strains (TIANGEN and TSINGKE) and regulate protein expression level by lacO ...
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bioRxiv - Synthetic Biology 2024Quote: ... the strain Top10 (TIANGEN biotech., Beijing, China) was transformed with the driver and reporter plasmids ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was isolated from single third-instar larva of three strains (total 30 larvae of each strain) using the DNA/RNA/Protein Isolation Kit (TianGen, Beijing, China). The first-strand cDNA was synthesized with 1 μg of total RNA using a PrimeScript™ 1st Strand cDNA Synthesis Kit with gDNA Eraser (TaKaRa Biotechnology ...
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bioRxiv - Microbiology 2022Quote: ... genomic DNA of strains and related Pf prophage mutation strains were isolated using the TIANamp Bacteria DNA kit (Tiangen Biotech Co. Ltd, Beijing, China) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: The genomic DNA of the isolated strain was extracted using a Bacterial DNA Kit (TIANGEN) and then submitted to Sangon Biotech (Shanghai ...
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bioRxiv - Neuroscience 2022Quote: ... The strains were expanded and cultured with a TIANprep Mini Plasmid Kit (DP103, Tiangen, China). The known standard curves were used to quantify the initial amount of the target template of the unknown sample ...
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bioRxiv - Microbiology 2019Quote: The genomic DNA of the sixteen strains were extracted using TIANamp Bacteria DNA Kit (TIANGEN, Beijing) following to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The genomic DNA of the strains was extracted using a TIANamp Bacteria DNA kit (Tiangen Biotech), using the manufacturer’s standard protocols ...
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bioRxiv - Plant Biology 2021Quote: Sequences for H2B.8 and H2B.2 were cloned into the pET28a+ vector with a non-cleavable C-terminal 8×His-tag and then transformed to Escherichia coli strain BL21 (Tiangen). Cells were grown to OD 0.8 at 37 °C in LB media with 30 μg/ml kanamycin ...
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bioRxiv - Neuroscience 2022Quote: ... The strains were expanded and cultured for endotoxin-free plasmid extraction with the EndoFree Maxi Plasmid Kit V2 (DP120, Tiangen, China). The constructed plasmids were stored at −20 °C for the transfection of HEK293-T cells (49).
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bioRxiv - Microbiology 2021Quote: The genomic DNA of the B3 strain was extracted using a Bacterial Genomic DNA Purification Kit (DP302-2, Tiangen Biotech Co., Beijing, China) according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2020Quote: ... Total RNA was extracted from mid-exponential cultures of strain HP1 (both the control and experimental groups) using the RNAprep Pure Bacteria Kit (Tiangen Biotech, Beijing, China) according to the manufacturer’s protocol ...
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bioRxiv - Zoology 2023Quote: Genomic DNA of the strain E2 was extracted using a TIANamp Bacterial DNA Kit according to the manufacturer’s protocol (Tiangen Biotech Co., LTD, Beijing, China). The PacBio and Illumina Miseq sequencing of the strain E2 genome and bioinformatic analysis were performed by Guangdong Magigene Biotechnology Co. ...
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bioRxiv - Cancer Biology 2021Quote: The RM-1 cells of 1×107 WT and Mettl9 KO were extracted with TRNzol (TIANGEN) for total RNA library preparation ...
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bioRxiv - Biochemistry 2020Quote: ... mixed with chloroform (1:1 v/v) using tubes with phase lock gel (Tiangen WM5-2302831). The aqueous phase was then mixed with 0.2 volume of 10 M ammonium acetate and 1 volume of isopropanol ...
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bioRxiv - Cell Biology 2022Quote: ... 1 unit of Taq DNA polymerase (TIANGEN, China), and 1 × PCR buffer ...
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bioRxiv - Immunology 2022Quote: ... Catch buffer: to 1 ml of RNAase-free water (Tiangen Biotech), add 40 µl Rnasin (NEB ...
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bioRxiv - Developmental Biology 2023Quote: Tissue and cells were homogenized with 1 mL TRIZOL (TIANGEN, DP424), after which 200 μL of chloroform was added to the lysates for phase separation ...
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bioRxiv - Cancer Biology 2020Quote: ... isopropyl β-D-1-thiogalactopyranoside (IPTG) and phenylmethane sulfonyl fluoride were purchased from Tiangen Biotech Co. ...
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bioRxiv - Immunology 2022Quote: ... cDNA synthesis was executed with 1 µg of total RNA with BcaBEST RNA PCR Kit (TIANGEN, China) as the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Total RNAs (1 μg) of experimental cells were extracted with the RNA simple kit (Tiangen, Beijing, China) and added to the reverse transcriptase reaction to obtain the first strand of cDNAs by using RevertAid First Strand cDNA Synthesis kit (K1622 ...
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bioRxiv - Plant Biology 2020Quote: ... AAA-CAA) within the PGEX-6P-1 plasmid was performed using the fast Site-Directed Mutagenesis Kit (Tiangen, China, Catalog No. KM101). PCR was conducted using site-specific primers (Supplemental Table S17) ...
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bioRxiv - Cancer Biology 2022Quote: ... Total RNA (1 µg) was used as a template for cDNA synthesis with a QuantScript RT Kit (KR103; Tiangen Biotech, Beijing, China). To determine mRNA expression levels ...
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bioRxiv - Bioengineering 2019Quote: ... The cell suspension was centrifuged for 5 minutes at 12,000 rpm and cell precipitate was resuspend in 1 ml sterilizing saline used for DNA extraction with TIANamp Genomic DNA kit (TianGen Biotechnologies, Beijing). Subsequently ...
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bioRxiv - Plant Biology 2022Quote: ... total RNAs isolated from the leaves of SC8 and mutant lines (#1, #2, #3, #4) using RNAplant Plus reagent (TianGen, Beijing, China) following the manufacturer’s instructions were reversed by using the reverse transcriptase kit (TaKaRa ...
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bioRxiv - Cancer Biology 2023Quote: RNA was isolated from 1×106 THP1 cells after various treatments (see above) by using an RNAprep pure cell/bacteria kit (Tiangen Biotech 4992235) according to the manufacturer’s instructions and was quantified using NanoDrop OneC Spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... the DNA fragments were separated by 1.0 % agarose gel electrophoresis (1 × TAE buffer) and purified from the gel with the DNA Gel Extraction Kit (TianGen Biotech., Beijing, China), then they were sub-cloned into pCE2 TA/Blunt-Zero vector (Vazyme ...
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bioRxiv - Plant Biology 2020Quote: ... Genomic DNA was extracted from the leaves of T3 transgenic plants (Table S8, lines 1954-1 and 1955-3) using a plant genomic DNA Extraction Kit (Tiangen Biotech, Beijing, China). About 20 μg of DNA was successfully digested with 5 U of EcoRV and incubated at 37 °C for 24 h ...
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bioRxiv - Microbiology 2022Quote: ... at which point 1 mL of cells were harvested and RNA samples were extracted using the RNAprep Pure Bacteria Kit (Tiangen Co., Beijing, China). Total cDNA was generated from 500 ng of total template RNA using the HiScript III All-in-one RT SuperMix Perfect for qPCR (Vazyme Co. ...