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Citations for TianGen Biotech :
1 - 34 of 34 citations for HLA A*0201 WT 1 complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: The RM-1 cells of 1×107 WT and Mettl9 KO were extracted with TRNzol (TIANGEN) for total RNA library preparation ...
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bioRxiv - Plant Biology 2023Quote: ... His-tag antibody (Tiangen, Beijing, China) and GST antibody (Tiangen).
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bioRxiv - Genomics 2023Quote: ... Total genomic DNA was extracted from fresh leaves using the Hi-DNAsecure Plant Kit (TIANGEN, China). Then ...
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bioRxiv - Genomics 2019Quote: Genomic DNA from WT and Prss53 mutant rabbits was isolated using a TIANamp Genomic DNA Kit (TIANGEN, Beijing, China). The DNA was amplified by 2×Taq Plus MasterMix (TIANGEN) ...
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bioRxiv - Physiology 2022Quote: ... Protein concentration was determined using BCA Protein Assay Kit (TIANGEN) and diluted in loading dye (β-mercaptoethanol 5% ...
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bioRxiv - Genomics 2023Quote: ... the genomic DNA was extracted from young leaves using the Hi-DNAsecure Plant Kit (cat. no. DP350; TIANGEN, China). Then ...
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bioRxiv - Immunology 2023Quote: ... hCD36 mice below refer to homozygous hCD36 mice) and wild-type (WT) C57BL/6J were harvested for extracting total RNA using RNAprep Pure Cell / Bacteria Kit (TIANGEN, DP430). The mRNA expression of hCD36 and mCD36 was determined using GAPDH as the internal control ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were quantified using a BCA Protein Assay Kit (TIANGEN, China). Western blotting analysis was performed according to previous studies [28 ...
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bioRxiv - Plant Biology 2021Quote: Sequences for H2B.8 and H2B.2 were cloned into the pET28a+ vector with a non-cleavable C-terminal 8×His-tag and then transformed to Escherichia coli strain BL21 (Tiangen). Cells were grown to OD 0.8 at 37 °C in LB media with 30 μg/ml kanamycin ...
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bioRxiv - Microbiology 2023Quote: PTrc His-A vector harboring various JEV NS2B-NS3 proteases were propagated in Escherichia coli DH5 (TIANGEN Biotech, Beijing, China) and extracted using Plasmid Miniprep Kit (Corning ...
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bioRxiv - Genetics 2024Quote: ... was isolated from fresh spermatozoa from the WT mice (n=3) and the Iqch KO mice (n=3) using the RNAsimple Total RNA Kit (Tiangen Biotech, China, 4992858). A Ribo-Zero™ rRNA Removal Kit (MRZPL1224 ...
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bioRxiv - Biochemistry 2020Quote: ... Protein concentration was measured using Bradford protein assay kit (Tiangen Biotech, Beijing Co., LTD, CN). The purity of the PM was determined by standard marker assays[32] ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... a BCA protein assay kit (TIANGEN, Beijing, China) was used to measure the membrane protein concentration ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were detected using an ECL fluorescence colorimetric kit (Tiangen) and visualized using a Bio-Rad Gel Doc XR ...
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bioRxiv - Cancer Biology 2023Quote: ... Human genome DNA extracted from HCT116 cells using the TIANamp Genomic DNA Kit (Tiangen Biotech, Beijing, China) was used as template for amplifying the promoter regions ...
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bioRxiv - Neuroscience 2023Quote: ... Total protein concentration was measured using a BCA assay kit (TIANGEN). Then 10 mg of protein per sample was separated on 12% SDS-PAGE gels (Sangon Biotech ...
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bioRxiv - Cell Biology 2023Quote: ... the chromatin sample concentration was assessed by the BCA Protein Assay Kit (Tiangen, cat no. PA115-02). 1 μg H3K27ac antibody (Abcam ab4729 ...
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bioRxiv - Biochemistry 2024Quote: ... The total protein in the supernatant was quantified using the bicinchoninic acid assay (BCA) kit (Beijing TianGen Biotechnology Co., Ltd.). For each sample ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was isolated from single third-instar larva of three strains (total 30 larvae of each strain) using the DNA/RNA/Protein Isolation Kit (TianGen, Beijing, China). The first-strand cDNA was synthesized with 1 μg of total RNA using a PrimeScript™ 1st Strand cDNA Synthesis Kit with gDNA Eraser (TaKaRa Biotechnology ...
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bioRxiv - Biochemistry 2020Quote: ... mixed with chloroform (1:1 v/v) using tubes with phase lock gel (Tiangen WM5-2302831). The aqueous phase was then mixed with 0.2 volume of 10 M ammonium acetate and 1 volume of isopropanol ...
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bioRxiv - Cell Biology 2022Quote: ... 1 unit of Taq DNA polymerase (TIANGEN, China), and 1 × PCR buffer ...
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bioRxiv - Immunology 2022Quote: ... Catch buffer: to 1 ml of RNAase-free water (Tiangen Biotech), add 40 µl Rnasin (NEB ...
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bioRxiv - Developmental Biology 2023Quote: Tissue and cells were homogenized with 1 mL TRIZOL (TIANGEN, DP424), after which 200 μL of chloroform was added to the lysates for phase separation ...
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bioRxiv - Cancer Biology 2020Quote: ... isopropyl β-D-1-thiogalactopyranoside (IPTG) and phenylmethane sulfonyl fluoride were purchased from Tiangen Biotech Co. ...
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bioRxiv - Immunology 2022Quote: ... cDNA synthesis was executed with 1 µg of total RNA with BcaBEST RNA PCR Kit (TIANGEN, China) as the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Total RNAs (1 μg) of experimental cells were extracted with the RNA simple kit (Tiangen, Beijing, China) and added to the reverse transcriptase reaction to obtain the first strand of cDNAs by using RevertAid First Strand cDNA Synthesis kit (K1622 ...
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bioRxiv - Plant Biology 2020Quote: ... AAA-CAA) within the PGEX-6P-1 plasmid was performed using the fast Site-Directed Mutagenesis Kit (Tiangen, China, Catalog No. KM101). PCR was conducted using site-specific primers (Supplemental Table S17) ...
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bioRxiv - Cancer Biology 2022Quote: ... Total RNA (1 µg) was used as a template for cDNA synthesis with a QuantScript RT Kit (KR103; Tiangen Biotech, Beijing, China). To determine mRNA expression levels ...
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bioRxiv - Bioengineering 2019Quote: ... The cell suspension was centrifuged for 5 minutes at 12,000 rpm and cell precipitate was resuspend in 1 ml sterilizing saline used for DNA extraction with TIANamp Genomic DNA kit (TianGen Biotechnologies, Beijing). Subsequently ...
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bioRxiv - Plant Biology 2022Quote: ... total RNAs isolated from the leaves of SC8 and mutant lines (#1, #2, #3, #4) using RNAplant Plus reagent (TianGen, Beijing, China) following the manufacturer’s instructions were reversed by using the reverse transcriptase kit (TaKaRa ...
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bioRxiv - Cancer Biology 2023Quote: RNA was isolated from 1×106 THP1 cells after various treatments (see above) by using an RNAprep pure cell/bacteria kit (Tiangen Biotech 4992235) according to the manufacturer’s instructions and was quantified using NanoDrop OneC Spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... the DNA fragments were separated by 1.0 % agarose gel electrophoresis (1 × TAE buffer) and purified from the gel with the DNA Gel Extraction Kit (TianGen Biotech., Beijing, China), then they were sub-cloned into pCE2 TA/Blunt-Zero vector (Vazyme ...
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bioRxiv - Plant Biology 2020Quote: ... Genomic DNA was extracted from the leaves of T3 transgenic plants (Table S8, lines 1954-1 and 1955-3) using a plant genomic DNA Extraction Kit (Tiangen Biotech, Beijing, China). About 20 μg of DNA was successfully digested with 5 U of EcoRV and incubated at 37 °C for 24 h ...
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bioRxiv - Microbiology 2022Quote: ... at which point 1 mL of cells were harvested and RNA samples were extracted using the RNAprep Pure Bacteria Kit (Tiangen Co., Beijing, China). Total cDNA was generated from 500 ng of total template RNA using the HiScript III All-in-one RT SuperMix Perfect for qPCR (Vazyme Co. ...