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Citations for TianGen Biotech :
1 - 39 of 39 citations for Ammonium Polyphosphate phase II 02 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... 100 μL of TMB peroxidase substrate (TIANGEN, PA107-02) was added to each well and incubated for 15 min before being stopped by adding 2 M H2SO4 ...
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bioRxiv - Developmental Biology 2023Quote: ... NF-H2O) and 10 μL proteinase K (20 mg/mL) (RT403-02, TIANGEN) was added to the slide and incubated for 10 min at room temperature ...
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bioRxiv - Synthetic Biology 2021Quote: ... and the DNA is extracted using the TIANgeo Midi Purification Kit (TIANGEN, DP209-02) or AxyPrep DNA gel Extraction Kit (AXYGEN ...
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bioRxiv - Genetics 2024Quote: ... Quantitative PCR was carried out using SuperReal PreMix Plus (SYBR Green) (Tiangen #DP4-02). RP49 were used to normalize results between groups ...
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bioRxiv - Microbiology 2023Quote: ... Each reaction contained 25 ul of SuperRealPreMix Plus SYBR Green (Tiangen Biotechnology, Beijing, FP205-02), one μl of cDNA ...
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bioRxiv - Genetics 2024Quote: Total RNA was extracted from cells and tissues using TRNzol Universal Reagent (Tiangen #DP4-02). 18S rRNA was obtained through polyacrylamide gel electrophoresis and gel recovery of total RNA ...
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bioRxiv - Synthetic Biology 2021Quote: ... plasmid extraction was performed following the manuals of the TIANprep Mini Plasmid Kit (TIANGEN, DP103-02) or AxyPrep Plasmid Miniprep Kit (AXYGEN ...
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bioRxiv - Microbiology 2020Quote: ... All the DNA samples were purified through a DNA kit column (DP214-02, Tiangen, Beijing, China) and kept at −20°C until further analysis (6) ...
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bioRxiv - Immunology 2020Quote: ... and total bacterial DNA was extracted using a bacterial DNA extraction kit (DP302-02, Tiangen, China) as per the manufacturer’s protocols ...
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bioRxiv - Genetics 2024Quote: Total RNA was extracted from cells and tissues using the TRNzol Universal Reagent (Tiangen #DP4-02). RNA reverse transcription was performed by using PrimeScript RT reagent Kit with gDNA Eraser (TAKARA #RR047A) ...
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bioRxiv - Microbiology 2022Quote: ... the supernatant was transferred to a phase lock gel tube (TIANGEN) containing 400 μl chloroform ...
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bioRxiv - Cell Biology 2024Quote: ... the chromatin sample concentration was assessed by the BCA Protein Assay Kit (Tiangen, cat no. PA115-02). 1 μg H3K27ac antibody (Abcam ab4729 ...
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bioRxiv - Plant Biology 2024Quote: ... The DNA was purified via a phase lock gel (Tiangen, Beijing, China). The precipitated DNA samples were quantified by qPCR using the respective pair of primers listed in Supplementary Table 4.
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bioRxiv - Biochemistry 2021Quote: ... An aliquot of 0.5 μg RNA were reverse transcribed to cDNA by FastKing RT Kit (KR116-02, Tiangen). cDNA samples were used as templates to perform real- time quantitative PCR with the power SYBR green mix (Abm ...
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bioRxiv - Immunology 2022Quote: ... cell pellets were re-suspended in 5 mL of red blood cell lysis buffer (RT122-02, TIANGEN, China) for 5 min at RT for remove the red blood cell ...
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bioRxiv - Molecular Biology 2020Quote: ... and cDNA transcription was accomplished using a first strand cDNA synthesis kit (Cat# KR116-02, Tiangen Biotech, China). Subsequently ...
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bioRxiv - Bioengineering 2021Quote: PC12 cell or rat liver gDNAs was isolated using the TIANamp blood DNA kit (TIANGEN Biotech, DP304-02) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cell pellets were re-suspended in 3 mL of red blood cell lysis buffer (RT122-02, TIANGEN, China) for 5 min at RT to remove the red blood cells ...
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bioRxiv - Synthetic Biology 2021Quote: ... The DNA fragments were separated by agarose gel electrophoresis and purified using a TIANgeo Midi Purification Kit (TIANGEN, DP209-02) or AxyPrep DNA gel Extraction Kit (AXYGEN ...
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bioRxiv - Microbiology 2020Quote: ... Total genomic DNA from each sample was extracted using the TIANamp Bacteria DNA isolation kit (DP302-02, Tiangen, Beijing, China). The quality of the extracted DNA was monitored on 1% agarose gels using electrophoresis and spectrophotometry (optical density at 260/280 nm ratio) ...
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bioRxiv - Molecular Biology 2020Quote: ... quantitative real-time PCR (qRT-PCR) was performed with SYBR green as fluorescence dye (Cat# FP205-02, Tiangen Biotech, China) in a total reaction volume of 20 μL according to the standard manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Protein concentrations were determined using the BCA Protein Assay Kit according to the manufacturer’s instructions (TIANGEN, catalog number: PA115-02).
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bioRxiv - Evolutionary Biology 2024Quote: ... was extracted from transfected HEK-293T from each subline (1×105) with the TIANamp genomic DNA Kit (DP304-02; Tiangen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Genome DNA from the collected cells of P2 was extracted under the instructions of the user’s manual for TIANamp Genomic DNA Kit (TIANGEN, DP304-02). PCR was later conducted to amplify mitochondrial COI gene fragment using the primer pair as follows ...
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bioRxiv - Plant Biology 2021Quote: ... and the leaf samples were pooled for each group for DNA extraction using the TIANGEN DNA purification kit (TIANGEN, DP305-02). High-throughput sequencing and Bulked Sequence Analysis (BSA ...
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bioRxiv - Genomics 2022Quote: ... on an agarose gel and were subsequently extracted using a Universal DNA purification Kit (Catalog No. DP214-02, TIANGEN, Beijing, China) and then used as a template to synthesize dsRNA with a MEGAscript RNAi Kit (Catalog No ...
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bioRxiv - Synthetic Biology 2021Quote: ... The incubated solution is then subjected to agarose gel electrophoresis and the correct band is extracted using a TIANgeo Midi Purification Kit (TIANGEN, DP209-02) or AxyPrep DNA gel Extraction Kit (AXYGEN ...
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bioRxiv - Synthetic Biology 2021Quote: ... The incubated solution is then subjected to agarose gel electrophoresis and the correct band is extracted using a TIANgeo Midi Purification Kit (TIANGEN, DP209-02) or AxyPrep DNA gel Extraction Kit (AXYGEN ...
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bioRxiv - Biochemistry 2020Quote: ... mixed with chloroform (1:1 v/v) using tubes with phase lock gel (Tiangen WM5-2302831). The aqueous phase was then mixed with 0.2 volume of 10 M ammonium acetate and 1 volume of isopropanol ...
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bioRxiv - Microbiology 2022Quote: ... The target N gene of GX_P2V isolate was amplified by qPCR using FastFire qPCR PreMix (Probe) (TIANGEN, Cat No. FP208-02, Beijing, China) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... DNAs were extracted by hydroxybenzene-chloroform-isoamyl alcohol and purified by a Phase Lock Gel (Tiangen, China). For ChIP-seq ...
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bioRxiv - Biochemistry 2022Quote: ... 1 μg of purified RNA was reverse-transcribed in a 20 µL reaction using a FastQuant RT Super Mix Kit (TIANGEN, catalog no. KR106-02). Real-Time PCR was then performed by using Maxima SYBR Green/ROX kit (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... After mixing with 400 μl chloroform and centrifugation in a Phase Lock Gel tube (#WM5-2302820, TIANGEN Biotech) the samples were centrifuged at 13,000 rpm for 15 min ...
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bioRxiv - Biochemistry 2024Quote: ... PCR was then performed using 2×Taq PCR MasterMix II (TIANGEN Biotech) and primers flanking m5C target sites ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Plasmids were extracted and purified using a TIANprep Mini Plasmid Kit II (Tiangen). The insertion and direction of probes were confirmed with Sanger sequencing using M13 PCR products (Tsingke) ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA was obtained according to the instructions of a TIANScript II RT kit (KR107, TIANGEN). The expression of transcripts of the target gene was measured by using a LightCycle® 96 instrument (Roche ...
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bioRxiv - Microbiology 2023Quote: ... Positive plasmid extracted from clones with correct sequence using TIANprep Mini Plasmid Kit II (Tiangen).
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bioRxiv - Cell Biology 2023Quote: ... Plasmid purification and extraction steps were carried out using the Endo Free Mini Plasmid Kit II (DP118, TIANGEN). ShRNA sequences are detailed in supplementary Figure 1 ...
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bioRxiv - Plant Biology 2024Quote: ... the sequence encoding full-length ICE1 was cloned into pET28a using ClonExpress II One Step Cloning Kit (Vazyme) and transformed into Escherichia coli strain BL21 (DE3) (TIANGEN). The protein was induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (IPTG ...