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Citations for TianGen Biotech :
1 - 41 of 41 citations for 7 CHLORO N N DIETHYL 4 NITRO 2 1 3 BENZOXADIAZOL 5 AMINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... was isolated from fresh spermatozoa from the WT mice (n=3) and the Iqch KO mice (n=3) using the RNAsimple Total RNA Kit (Tiangen Biotech, China, 4992858). A Ribo-Zero™ rRNA Removal Kit (MRZPL1224 ...
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bioRxiv - Plant Biology 2022Quote: ... total RNAs isolated from the leaves of SC8 and mutant lines (#1, #2, #3, #4) using RNAplant Plus reagent (TianGen, Beijing, China) following the manufacturer’s instructions were reversed by using the reverse transcriptase kit (TaKaRa ...
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bioRxiv - Plant Biology 2021Quote: ... and pUBQ10:MSD2ΔSP-mCherry. Mutagenesis of MSD2/Y68F was conducted with the Fast Site-Directed Mutagenesis Kit (cat. n. KM101) from TianGen. The constructs were introduced into plants by the floral dip method [21] ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 5 ul 2×Taq PCR mix (TIANGEN, China), 0.5 ul each primer (trnL and trnF (Taberlet et al. ...
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bioRxiv - Microbiology 2022Quote: ... The target N gene of GX_P2V isolate was amplified by qPCR using FastFire qPCR PreMix (Probe) (TIANGEN, Cat No. FP208-02, Beijing, China) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: RNA was extracted from mouse brain lysates (mixture of 3∼4 mouse brains for each sample) using RNAprep pure Tissue Kit (Tiangen, DP431). cDNA was synthesized using HiScript II 1st Strand cDNA Synthesis Kit (Vazyme ...
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bioRxiv - Cell Biology 2021Quote: ... the first batch of cells (5×106) were collected 2 days after infection using the TIANamp genomic DNA kit (Tiangen, DP304), and the second batch of cells treated with puromycin were collected at 7d ...
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bioRxiv - Plant Biology 2020Quote: ... Genomic DNA was extracted from the leaves of T3 transgenic plants (Table S8, lines 1954-1 and 1955-3) using a plant genomic DNA Extraction Kit (Tiangen Biotech, Beijing, China). About 20 μg of DNA was successfully digested with 5 U of EcoRV and incubated at 37 °C for 24 h ...
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bioRxiv - Plant Biology 2021Quote: The roots of 7 days old seedlings were collected to extract total RNA using RNA prep pure plant kit (TIANGEN). The first strand cDNA was synthesized from 1 μg of total RNA using the Hifair® III 1st Strand cDNA Synthesis SuperMix kit (YEASEN ...
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bioRxiv - Microbiology 2021Quote: ... 2×Taq PCR Mix was purchased from TIANGEN Biotech (Beijing ...
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bioRxiv - Genomics 2019Quote: ... The DNA was amplified by 2×Taq Plus MasterMix (TIANGEN), and the PCR primers used to detect mutations were as follows ...
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bioRxiv - Molecular Biology 2022Quote: ... Total RNA of 3-week-old SsSINA1a-overexpressing Arabidopsis was extracted using an RNA Extraction Kit (DP411, Tiangen Biotech). The housekeeping genes AtACTIN was used as the internal control ...
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bioRxiv - Genetics 2022Quote: ... PCR reactions were performed by using 2×Taq PCR Master Mix (TIANGEN, China). KASP marker analysis was performed on the StepOnePlus Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Immunology 2022Quote: ... cell pellets were re-suspended in 5 mL of red blood cell lysis buffer (RT122-02, TIANGEN, China) for 5 min at RT for remove the red blood cell ...
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bioRxiv - Microbiology 2019Quote: ... PCR mixture contained 12 μL of 2× Taq PCR Mix (TIANGEN Co. Ltd., Beijing, PR China), 1 μL DNA template ...
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bioRxiv - Microbiology 2024Quote: ... The real-time quantitative PCR (q-PCR) (ABI7500, USA) using SYBR 2×RealUniversal PreMix (Tiangen Biotech). qPCR was performed to assess the abundance of the macrolide ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was extracted from less than 100 mg of 10-d-old seedlings that were grown vertically on half-strength MS plates or treated with 50 μM AA or an equal volume of DMSO for 4 h in liquid MS medium using RNAprep Pure Plant Plus Kit (TIANGEN, DP441). First-strand cDNA was synthesized using a HiScript® II cDNA Synthesis Kit (Vazyme R212-02 ...
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bioRxiv - Microbiology 2021Quote: ... of strain TC967 was extracted from 4-day-old mycelia following the protocol described in the handbook of the Plant Genomic DNA Kit (Tiangen, Beijing, China). The gDNA was sequenced by Illumina HisSeq 2500 sequencing (Illumina ...
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bioRxiv - Genomics 2023Quote: Total RNA was extracted from the developing leaves of NTS57 under normal conditions (CK) or 12 h after -4℃ treatment using the TRIzol method (Tiangen, Beijing, China) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... All cells in each well were harvested 3 days post-transfection and the genomic DNA was extracted by TIANamp Genomic DNA Kit (TIANGEN Biotech Co., Ltd., Beijing, China) following the manufacturer’s instructions.
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bioRxiv - Biochemistry 2020Quote: ... 2 μg of total RNA were reverse transcribed into cDNA using the FastGeneScriptase Basic cDNA Kit (Tiangen Biotech) with random Oligo(dT ...
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bioRxiv - Molecular Biology 2020Quote: ... Isolates were cultured on Middlebrook medium for 4-6 weeks at 37°C and DNA was extracted using magnetic beads (Tiangen Biotech Co., Ltd.). Rifampicin and isoniazid drug-susceptibility testing was performed using the proportion method in Löwenstein-Jensen medium (Aziz et al ...
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bioRxiv - Neuroscience 2022Quote: ... 2 ug RNA per sample were used to reverse transcript the cDNA by QuantScript RT Kit (TIANGEN Biotech, KR103). 2 x iTaq Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: The RM-1 cells of 1×107 WT and Mettl9 KO were extracted with TRNzol (TIANGEN) for total RNA library preparation ...
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bioRxiv - Biochemistry 2020Quote: ... mixed with chloroform (1:1 v/v) using tubes with phase lock gel (Tiangen WM5-2302831). The aqueous phase was then mixed with 0.2 volume of 10 M ammonium acetate and 1 volume of isopropanol ...
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bioRxiv - Plant Biology 2019Quote: SSR-PCR amplification reactions were conducted using a total volume of 10 µl consisting of 5µl of 2×Taq PCR MasterMix (Tiangen, Beijing, China) (0.1 U/µl Taq DNA Polymerase ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA was extracted from cultured cells by using TRIZOL reagent and 500 ng to 2 μg RNA was reversely transcribed into first-strand cDNA by FastKing RT Kit (Tiangen, KR116). qPCR analysis was performed with Mastercycler Realplex2 (Eppendorf ...
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bioRxiv - Genetics 2024Quote: ... First-strand cDNA was synthesized from 2 μg of DNase I-treated total RNA using the FastKing RT kit (TIANGEN, KR116). qRT-PCR was performed using the ChamQ Universal SYBR qPCR Master Mix (Vazyme ...
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bioRxiv - Microbiology 2021Quote: The genomic DNA of the B3 strain was extracted using a Bacterial Genomic DNA Purification Kit (DP302-2, Tiangen Biotech Co., Beijing, China) according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2022Quote: ... 1 unit of Taq DNA polymerase (TIANGEN, China), and 1 × PCR buffer ...
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bioRxiv - Immunology 2022Quote: ... Catch buffer: to 1 ml of RNAase-free water (Tiangen Biotech), add 40 µl Rnasin (NEB ...
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bioRxiv - Developmental Biology 2023Quote: Tissue and cells were homogenized with 1 mL TRIZOL (TIANGEN, DP424), after which 200 μL of chloroform was added to the lysates for phase separation ...
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bioRxiv - Cancer Biology 2020Quote: ... isopropyl β-D-1-thiogalactopyranoside (IPTG) and phenylmethane sulfonyl fluoride were purchased from Tiangen Biotech Co. ...
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bioRxiv - Immunology 2022Quote: ... cDNA synthesis was executed with 1 µg of total RNA with BcaBEST RNA PCR Kit (TIANGEN, China) as the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Total RNAs (1 μg) of experimental cells were extracted with the RNA simple kit (Tiangen, Beijing, China) and added to the reverse transcriptase reaction to obtain the first strand of cDNAs by using RevertAid First Strand cDNA Synthesis kit (K1622 ...
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bioRxiv - Plant Biology 2020Quote: ... AAA-CAA) within the PGEX-6P-1 plasmid was performed using the fast Site-Directed Mutagenesis Kit (Tiangen, China, Catalog No. KM101). PCR was conducted using site-specific primers (Supplemental Table S17) ...
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bioRxiv - Cancer Biology 2022Quote: ... Total RNA (1 µg) was used as a template for cDNA synthesis with a QuantScript RT Kit (KR103; Tiangen Biotech, Beijing, China). To determine mRNA expression levels ...
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bioRxiv - Bioengineering 2019Quote: ... The cell suspension was centrifuged for 5 minutes at 12,000 rpm and cell precipitate was resuspend in 1 ml sterilizing saline used for DNA extraction with TIANamp Genomic DNA kit (TianGen Biotechnologies, Beijing). Subsequently ...
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bioRxiv - Cancer Biology 2023Quote: RNA was isolated from 1×106 THP1 cells after various treatments (see above) by using an RNAprep pure cell/bacteria kit (Tiangen Biotech 4992235) according to the manufacturer’s instructions and was quantified using NanoDrop OneC Spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... the DNA fragments were separated by 1.0 % agarose gel electrophoresis (1 × TAE buffer) and purified from the gel with the DNA Gel Extraction Kit (TianGen Biotech., Beijing, China), then they were sub-cloned into pCE2 TA/Blunt-Zero vector (Vazyme ...
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bioRxiv - Microbiology 2022Quote: ... at which point 1 mL of cells were harvested and RNA samples were extracted using the RNAprep Pure Bacteria Kit (Tiangen Co., Beijing, China). Total cDNA was generated from 500 ng of total template RNA using the HiScript III All-in-one RT SuperMix Perfect for qPCR (Vazyme Co. ...