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Citations for TianGen Biotech :
1 - 22 of 22 citations for 6 Fluoro 1 3 benzodioxene 8 carbonyl chloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... total RNAs isolated from the leaves of SC8 and mutant lines (#1, #2, #3, #4) using RNAplant Plus reagent (TianGen, Beijing, China) following the manufacturer’s instructions were reversed by using the reverse transcriptase kit (TaKaRa ...
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bioRxiv - Plant Biology 2020Quote: ... Genomic DNA was extracted from the leaves of T3 transgenic plants (Table S8, lines 1954-1 and 1955-3) using a plant genomic DNA Extraction Kit (Tiangen Biotech, Beijing, China). About 20 μg of DNA was successfully digested with 5 U of EcoRV and incubated at 37 °C for 24 h ...
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bioRxiv - Genetics 2024Quote: ... was isolated from fresh spermatozoa from the WT mice (n=3) and the Iqch KO mice (n=3) using the RNAsimple Total RNA Kit (Tiangen Biotech, China, 4992858). A Ribo-Zero™ rRNA Removal Kit (MRZPL1224 ...
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bioRxiv - Plant Biology 2021Quote: Sequences for H2B.8 and H2B.2 were cloned into the pET28a+ vector with a non-cleavable C-terminal 8×His-tag and then transformed to Escherichia coli strain BL21 (Tiangen). Cells were grown to OD 0.8 at 37 °C in LB media with 30 μg/ml kanamycin ...
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bioRxiv - Molecular Biology 2020Quote: ... Isolates were cultured on Middlebrook medium for 4-6 weeks at 37°C and DNA was extracted using magnetic beads (Tiangen Biotech Co., Ltd.). Rifampicin and isoniazid drug-susceptibility testing was performed using the proportion method in Löwenstein-Jensen medium (Aziz et al ...
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bioRxiv - Molecular Biology 2022Quote: ... Total RNA of 3-week-old SsSINA1a-overexpressing Arabidopsis was extracted using an RNA Extraction Kit (DP411, Tiangen Biotech). The housekeeping genes AtACTIN was used as the internal control ...
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bioRxiv - Neuroscience 2021Quote: RNA was extracted from mouse brain lysates (mixture of 3∼4 mouse brains for each sample) using RNAprep pure Tissue Kit (Tiangen, DP431). cDNA was synthesized using HiScript II 1st Strand cDNA Synthesis Kit (Vazyme ...
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bioRxiv - Synthetic Biology 2022Quote: ... All cells in each well were harvested 3 days post-transfection and the genomic DNA was extracted by TIANamp Genomic DNA Kit (TIANGEN Biotech Co., Ltd., Beijing, China) following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: The RM-1 cells of 1×107 WT and Mettl9 KO were extracted with TRNzol (TIANGEN) for total RNA library preparation ...
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bioRxiv - Biochemistry 2020Quote: ... mixed with chloroform (1:1 v/v) using tubes with phase lock gel (Tiangen WM5-2302831). The aqueous phase was then mixed with 0.2 volume of 10 M ammonium acetate and 1 volume of isopropanol ...
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bioRxiv - Cell Biology 2022Quote: ... 1 unit of Taq DNA polymerase (TIANGEN, China), and 1 × PCR buffer ...
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bioRxiv - Immunology 2022Quote: ... Catch buffer: to 1 ml of RNAase-free water (Tiangen Biotech), add 40 µl Rnasin (NEB ...
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bioRxiv - Developmental Biology 2023Quote: Tissue and cells were homogenized with 1 mL TRIZOL (TIANGEN, DP424), after which 200 μL of chloroform was added to the lysates for phase separation ...
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bioRxiv - Cancer Biology 2020Quote: ... isopropyl β-D-1-thiogalactopyranoside (IPTG) and phenylmethane sulfonyl fluoride were purchased from Tiangen Biotech Co. ...
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bioRxiv - Immunology 2022Quote: ... cDNA synthesis was executed with 1 µg of total RNA with BcaBEST RNA PCR Kit (TIANGEN, China) as the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Total RNAs (1 μg) of experimental cells were extracted with the RNA simple kit (Tiangen, Beijing, China) and added to the reverse transcriptase reaction to obtain the first strand of cDNAs by using RevertAid First Strand cDNA Synthesis kit (K1622 ...
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bioRxiv - Plant Biology 2020Quote: ... AAA-CAA) within the PGEX-6P-1 plasmid was performed using the fast Site-Directed Mutagenesis Kit (Tiangen, China, Catalog No. KM101). PCR was conducted using site-specific primers (Supplemental Table S17) ...
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bioRxiv - Cancer Biology 2022Quote: ... Total RNA (1 µg) was used as a template for cDNA synthesis with a QuantScript RT Kit (KR103; Tiangen Biotech, Beijing, China). To determine mRNA expression levels ...
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bioRxiv - Bioengineering 2019Quote: ... The cell suspension was centrifuged for 5 minutes at 12,000 rpm and cell precipitate was resuspend in 1 ml sterilizing saline used for DNA extraction with TIANamp Genomic DNA kit (TianGen Biotechnologies, Beijing). Subsequently ...
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bioRxiv - Cancer Biology 2023Quote: RNA was isolated from 1×106 THP1 cells after various treatments (see above) by using an RNAprep pure cell/bacteria kit (Tiangen Biotech 4992235) according to the manufacturer’s instructions and was quantified using NanoDrop OneC Spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... the DNA fragments were separated by 1.0 % agarose gel electrophoresis (1 × TAE buffer) and purified from the gel with the DNA Gel Extraction Kit (TianGen Biotech., Beijing, China), then they were sub-cloned into pCE2 TA/Blunt-Zero vector (Vazyme ...
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bioRxiv - Microbiology 2022Quote: ... at which point 1 mL of cells were harvested and RNA samples were extracted using the RNAprep Pure Bacteria Kit (Tiangen Co., Beijing, China). Total cDNA was generated from 500 ng of total template RNA using the HiScript III All-in-one RT SuperMix Perfect for qPCR (Vazyme Co. ...