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Citations for System Biosciences :
1 - 38 of 38 citations for Calcium calmodulin Dependent 3' 5' Cyclic Nucleotide Phosphodiesterase 1C PDE1C Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... An in vitro transcribed sgRNA targeting Slc16a11 (5’-AGTCCTAACCTCGCTTGGCT-3’) and hspCas9 mRNA (CAS500A-1, System Biosciences) were microinjected into C57BL/6J mouse zygotes by the Genome Modification Facility at Harvard University ...
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bioRxiv - Molecular Biology 2023Quote: ... a minimal piggyBac cargo construct was created by deleting all intervening sequences between the 5’ and 3’ terminal repeats (including core insulators) of the PB-CMV-MCS-EF1α-Puro vector (System Biosciences, PB510B-1). A gBlock (Integrated DNA Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... The membrane was blocked in freshly prepared 5% BSA diluted in TBS before being immunoblotted with specific primary antibodies (CD81; #EXOAB-CD81A-1 System Biosciences, GM130 ...
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bioRxiv - Synthetic Biology 2020Quote: ... The human proteins were assigned to 3 Piggybac vectors (System Biosciences, USA) by Golden Gate Assembly method (11 ...
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bioRxiv - Molecular Biology 2021Quote: ... with 1/5 final dilution (System Biosciences, LV810A-1), and the mixture was incubated at 4 °C for 6 hrs ...
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bioRxiv - Developmental Biology 2020Quote: ... 4 mL 5× PEG reagent (System Biosciences, LV825A-1) was added and mixed by pipetting ...
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bioRxiv - Molecular Biology 2020Quote: ... The WPRE 3’-UTR sequence was amplified from the CD813A-1 (System Biosciences) vector.
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... Virus was concentrated using 5× PEG- it™ (System Biosciences) as per manufacturer’s protocol and virus pellet obtained was aliquoted in opti-MEM and stored at -80°C ...
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bioRxiv - Cell Biology 2023Quote: ... and 5 ng μL-1 Cas9 mRNA (System Biosciences, Palo Alto, CA) in 10 mM Tris-HCl pH 8.0 and 0.1 mM EDTA were used for injection into the pronucleus of fertilized mouse zygotes ...
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bioRxiv - Cancer Biology 2019Quote: ... and anti-CD81 antibodies (System Biosciences, CA).
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bioRxiv - Molecular Biology 2021Quote: ... The WPRE (Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element) 3’-UTR sequence was amplified from the CD813A-1 (System Biosciences) vector.
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bioRxiv - Genetics 2019Quote: ... matched with Zfr knockdown: 5’-AACCGATGTACTTCCCGTTAAT) were cloned into the pGreenPuro backbone from System Biosciences. This construct was used to produce a 6-well of lentivirus according to standard protocols in 293T cells using the third-generation system and concentrated 1:100 with lenti-X (Clontec) ...
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bioRxiv - Physiology 2023Quote: ... and 5 µL of de-fibrination reagent (Cat #TMEXO-1, System Biosciences, Palo Alto, CA) was added to the plasma to remove clotting factors prior to the isolation of EVs ...
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bioRxiv - Developmental Biology 2022Quote: ... The gRNA (5 pg/embryo) and mRNA encoding Cas9 protein (150 pg/embryo, System Biosciences) were injected into one-cell stage embryos ...
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bioRxiv - Immunology 2021Quote: ... 5’ ATACGCGGATCCtcagtagggccgccacac 3’ and cloned into a commercial lentivector containing a GFP reporter gene (pCDH-GFP-Em-CD513B-1 from System Biosciences). To overexpress RUNX2 in human T cells ...
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bioRxiv - Immunology 2021Quote: ... Construct expressing lipin-1-mCherry was amplified by PCR adding 5’Xba-I and Sal-I 3’specific restriction sites and inserted into lentiviral vector pCDH-CMV-MCS-EF1α-copGFP (System Biosciences) after removing an internal Sal-I restriction site by mutagenesis ...
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bioRxiv - Microbiology 2021Quote: ... media were changed to DMEM supplemented with 5% exosome-depleted FBS (System Biosciences, Palo Alto, CA, USA). After 48 h ...
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bioRxiv - Cancer Biology 2019Quote: ... CD63 antibody was purchased from System Biosciences (Palo Alto, CA, USA). β-actin antibody was purchased from Sigma-Aldrich (St ...
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bioRxiv - Biochemistry 2020Quote: ... anti-TSG101 antibody (EXOAB-TSG101-1; System Biosciences, CA; 1:500), anti-CD63 antibody (EXOAB-CD63A-1 ...
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bioRxiv - Biochemistry 2020Quote: ... anti-CD63 antibody (EXOAB-CD63A-1; System Biosciences, CA; 1:500), anti-CD5L antibody (AF2834 ...
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bioRxiv - Developmental Biology 2020Quote: ... around the sgRNA1 target site at +9bp from the start of TBX5 exon 3 was cloned by Cold Fusion (System Biosciences #MC010B) using amplicons from genomic DNA of WTC11 into a construct that was a modification of plasmid pEN114 (Nora et al. ...
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bioRxiv - Cell Biology 2022Quote: ... The NTA measurements rely on light scattering to extract particle size and the number of particles in a sample and the NTA software (Version 2.3) collects data on multiple particles to calculate the hydrodynamic diameter of each particle using the Stokes-Einstein equation (System Biosciences, LLC).
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bioRxiv - Neuroscience 2022Quote: ... 500 μL of human plasma were incubated with 5 μL purified thrombin (System Biosciences, Inc.; Catalog # TMEXO-1) at room temperature for 5 min ...
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bioRxiv - Cancer Biology 2022Quote: ... we performed the Exo-check Antibody Array (System Biosciences, Palo Alto CA, #EXORAY210A). 30 μg of EV protein was aliquoted and immunoblots were processed and developed according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2021Quote: ... transferred onto nitrocellulose membranes and blotted with the following antibodies: CD9 (System Bioscience) and GM130 (Abcam ...
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bioRxiv - Cancer Biology 2020Quote: Tissues were stained with antibody for anti-CD63 (System Bioscience, Mountain View, CA, USA). The secondary antibody ...
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bioRxiv - Cancer Biology 2021Quote: ... GM130 and ICAM by immunoblot using the Exo-Check Exosome Antibody Array (System Biosciences, #EXORAY210A). Protein concentration for EV samples was measured using BCA (Thermo Fisher ...
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bioRxiv - Bioengineering 2022Quote: ... and Exo-Check™ exosome antibody array (EXORAY200B-4) were purchased from SBI (System Biosciences). miRCURY LNA™ RT kit ...
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bioRxiv - Microbiology 2021Quote: ... EBV-negative Akata(-) cells were treated with purified exosomes in RPMI1640 supplemented with 5% exosome-depleted FBS (System Biosciences, Palo Alto, CA, USA). After 6 h ...
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bioRxiv - Neuroscience 2020Quote: ... fibrinogen and fibrin proteins known to impede efficient EV recovery were removed using 5 U/mL of thrombin (System Biosciences, Inc.; Mountainview, CA) for 30 minutes at RT followed by addition of 495 μL of Dulbecco’s PBS-1X (DPBS ...
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bioRxiv - Genetics 2023Quote: ... 6 X 105 cells were co-transfected with 5 µg of gRNA-containing piggyBac vector and 1 µg of piggyBac transposase plasmid (System Biosciences PB210PA-1) using Lipofectamine 3000 Transfection Reagent ...
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bioRxiv - Bioengineering 2023Quote: Western blotting was performed using ExoA Antibody Kit according to the manufacturer’s protocol (System Biosciences, USA). Immunoblots were cut prior to primary antibody hybridization ...
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bioRxiv - Bioengineering 2023Quote: ... and anti-rabbit HRP-linked secondary antibody (EXOAB-KIT-1) were purchased from System Biosciences (CA, USA). Anti-beta-actin antibody (sc-47778) ...
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bioRxiv - Cell Biology 2021Quote: ... Virus-containing supernatants were either collected or concentrated by mixing with quarter volumes of 5 × PEG-it Virus Precipitation Solution (System Biosciences, Palo Alto, CA, United States), followed by centrifugation according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... for 1 h at room temperature and incubated with primary antibodies overnight at 4 °C with anti-Cd9 ([1:3000; System Biosciences, Palo Alto ...
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bioRxiv - Synthetic Biology 2020Quote: ... Exo-Check Array: A549 protein markers were characterised using a commercially sourced Exo-Check Exosome Antibody Array kit (#EXORAY200A-4, System Biosciences, CA, USA). A549 EVs (100 μg ...
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bioRxiv - Cell Biology 2019Quote: ... the collected exosomes were further purified with anti-CD9 antibody-coated magnetic beads (CD9 Exo-Flow Exosome Purification Kit, System Biosciences, Cat. EXOTC10A-1) as in (Rim and Kim ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated overnight at 4°C with 1:1000 primary antibody (CD63 and CD81, rabbit anti-human, EXOAB-CD63A-1/ EXOAB-CD81A-1, System Biosciences, Palo Alto, CA, USA) in TBST ...