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Citations for System Biosciences :
1 - 19 of 19 citations for C Reactive Protein Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... using a 5’ primer carrying NsiI and a 3’ primer carrying P2A-NsiI and ligated into the NsiI digested pCDH-SFFV-GLuc-T2A-mCherry vector downstream of the T2A peptide (Figure S2a) (pCDH-vector, System Bioscience). For inducible knockdown of target genes ...
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bioRxiv - Genomics 2019Quote: ... Cas9 protein was bought from System Biosciences (2438 Embarcadero Way ...
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bioRxiv - Molecular Biology 2022Quote: ... pT-mAppleT2Apuro was generated by PCR amplifying the T2A peptide and puromycin resistance gene from PB-CMV-MCS-GreenPuro (System Biosciences, Cat# PB513B-1) and cloning into pT-mApple (Vectorbuilder) ...
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bioRxiv - Microbiology 2020Quote: ... for 2 h at 37°C and plasmid-safe DNase (System Bioscience) overnight at 37°C ...
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bioRxiv - Synthetic Biology 2020Quote: ... The human proteins were assigned to 3 Piggybac vectors (System Biosciences, USA) by Golden Gate Assembly method (11 ...
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bioRxiv - Developmental Biology 2022Quote: ... The gRNA (5 pg/embryo) and mRNA encoding Cas9 protein (150 pg/embryo, System Biosciences) were injected into one-cell stage embryos ...
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bioRxiv - Molecular Biology 2021Quote: ... exosomes from ASCs were labeled fluorescent red using an ExoGlow-Protein EV Labeling Kit (System Biosciences) and then incubated with precipitation solution at 4°C overnight ...
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bioRxiv - Genomics 2020Quote: ... overnight at 37°C and cloned into a linearized pCDH-EF1alpha-MCS-BGH-PGK-GFP-T2A-Puro lentivector (CD550A-1, System Biosciences) using T4 DNA ligase (New England BioLabs ...
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bioRxiv - Neuroscience 2021Quote: ... Lentiviral particles were harvested either by ultracentrifugation at 106,559 g for 2 h at 4°C using a Beckman SW 32 Ti rotor or using the PEG-it Virus Precipitation Solution (System Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... for 1 h at room temperature and incubated with primary antibodies overnight at 4 °C with anti-Cd9 ([1:3000; System Biosciences, Palo Alto ...
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bioRxiv - Biochemistry 2023Quote: ... the ERIGIC-53 gene with a C-terminal PA tag was inserted into a PiggyBac Cumate Switch Inducible vector (System Bioscience). The pcDNA-SP-PA-ERGIC-53 plasmid was generated using the In-Fusion cloning kit (Taraka ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated overnight at 4°C with 1:1000 primary antibody (CD63 and CD81, rabbit anti-human, EXOAB-CD63A-1/ EXOAB-CD81A-1, System Biosciences, Palo Alto, CA, USA) in TBST ...
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bioRxiv - Cancer Biology 2020Quote: ... Double-stranded oligonucleotides were cloned into the linearized SmartNuclease vector (EF1-T7-hspCas9-T2A-RFP-H1-gRNA with red fluorescent protein (RFP, System Biosciences) and IFNLR1-guide RNA (IFNLR1-gRNA ...
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bioRxiv - Cell Biology 2022Quote: For stable expression of mScarlet (mSc) tagged IFT-A proteins (IFT144, IFT140, IFT122, IFT121, and IFT139) and associated mutants using the Super PiggyBac system (System Biosciences), the relevant open reading frames were inserted into the PB-EF1α-MCS-IRES-Neo vector with C-terminal TEV ...
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bioRxiv - Synthetic Biology 2020Quote: ... and then transfected with three piggyBac vectors expressing multiple human proteins with the Neon Transfection System with the presence of piggyBac Transposase (System Biosciences, USA). Two days after ...
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bioRxiv - Synthetic Biology 2020Quote: ... Exo-Check Array: A549 protein markers were characterised using a commercially sourced Exo-Check Exosome Antibody Array kit (#EXORAY200A-4, System Biosciences, CA, USA). A549 EVs (100 μg ...
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bioRxiv - Neuroscience 2020Quote: ... fibrinogen and fibrin proteins known to impede efficient EV recovery were removed using 5 U/mL of thrombin (System Biosciences, Inc.; Mountainview, CA) for 30 minutes at RT followed by addition of 495 μL of Dulbecco’s PBS-1X (DPBS ...
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bioRxiv - Molecular Biology 2019Quote: The identification of protein markers on the isolated exosomes was undertaken using the commercially available Exo-Check Exosome Ab Array kit (System Biosciences, Palo Alto, CA), as described by the manufacturer ...
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bioRxiv - Developmental Biology 2022Quote: ... with 250 μg protein equivalent of AFSC-EVs stained using 2 μL of ExoGlow™-Vivo (Near IR) EV Labeling Kit (System Biosciences, Palo Alto, CA).